Development of microsatellite markers for bambara groundnut (Vigna subterranea L. Verdc.) — an underutilized African legume crop species

Bambara groundnut ( Vigna subterranea (L.) Verdc.) is an important African legume crop. In this study, a collection consisting of 240 accessions was analyzed using 22 simple sequence repeat (SSR) markers. In total, 166 alleles were detected, with a mean of 7.59 alleles per locus. Allelic and gene diversities were higher in the west African and Cameroon/Nigeria regions with 6.68 and 6.18 alleles per locus, and 0.601 and 0.571, respectively. The genetic distance showed high similarity between west African and Cameroon/Nigeria accessions. Principal coordinate analyses and neighbor-joining analysis consistently revealed that the majority of west African accessions were grouped with Cameroon/Nigeria accessions, but they were differentiated from east African, central African, and southeast Asian accessions. Population structure analysis showed that two subpopulations existed, and most of the east African accessions were restricted to one subpopulation with some Cameroon/Nigeria accessions, whereas most of the west African accessions were associated with most of the Cameroon/Nigeria accessions in the other subpopulation. Comparison with SSR analysis of other Vigna cultigens, i.e., cultivated azuki bean ( Vigna angularis ) and mungbean ( Vigna radiata ), reveals that the mean gene diversity of Bambara groundnut was lower than azuki bean but higher than mungbean.

Download Full-text

Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

Cytogenetic and Genome Research ◽

10.1159/000521398 ◽

2022 ◽

pp. 1-9

Author(s):

Ahmet L. Tek ◽

Sevim D. Kara Öztürk ◽

Hümeyra Yıldız ◽

Didem Karalar

Keyword(s):

Dna Sequences ◽

Bambara Groundnut ◽

Single Pair ◽

Vigna Subterranea ◽

Telomeric Dna ◽

Legume Crop ◽

Climate Resilience ◽

Subtelomeric Repeat ◽

Rna Genes ◽

Potential Use

Bambara groundnut (Vigna subterranea L. Verdc.) is an underutilized minor legume crop with climate resilience and great potential use in world agriculture. This study aimed to cytogenetically characterize the genome and chromosome properties of Bambara groundnut. We cloned, sequenced, and mapped a 50-bp centromere-specific tandem repeat on all chromosomes. In addition, a 400-bp subtelomeric repeat was discovered and mapped on a single pair of chromosomes. A Bambara groundnut karyotype was constructed using these novel repeats along with ribosomal RNA genes (45S and 5S) and telomeric DNA sequences. This study provides the first analysis of the genome and chromosome properties of Bambara groundnut. We discuss our findings in relation to genetic improvement of Bambara groundnut and centromere evolution in legume species.

Download Full-text

INFLUENCE OF SOWING DATE AND ENVIRONMENTAL FACTORS ON THE DEVELOPMENT AND YIELD OF BAMBARA GROUNDNUT (VIGNA SUBTERRANEA) LANDRACES IN A SUB-TROPICAL REGION

Experimental Agriculture ◽

10.1017/s0014479708006145 ◽

2008 ◽

Vol 44 (2) ◽

pp. 167-183 ◽

Cited By ~ 3

Author(s):

A. SESAY ◽

C. N. MAGAGULA ◽

A. B. MANSUETUS

Keyword(s):

Environmental Factors ◽

Rural Areas ◽

Field Experiments ◽

Sowing Date ◽

Bambara Groundnut ◽

Effect Of Temperature ◽

Grain Legume ◽

Tropical Region ◽

Vigna Subterranea ◽

Legume Crop

SUMMARYBambara groundnut (Vigna subterranea) is a grain legume crop, which is increasingly popular as food in rural areas across the African continent. However, reliable information pertaining to management of the crop is limited. Field experiments were conducted in Swaziland in the 1998/99 and 1999/2000 cropping seasons to determine the influence of sowing date and environmental factors on the growth, development and yield of bambara groundnut. In the 1998/99 season, seeds of one local landrace were sown on six dates between mid-September 1998 and mid-February 1999. In 1999/2000, seeds of two local landraces were sown on six dates between mid-October 1999 and late January 2000. The highest pod yield and total dry matter production were achieved in November sowings, with maximum pod yields of 1.3 and 0.64 t ha−1 for the 1998/99 and 1999/2000 seasons, respectively. Earlier sowing and successive delays in sowing from November caused substantial yield declines of as much as 72–75%. Sowing date influenced yields of bambara groundnut through the effect of temperature and daylength on plant development. For the landraces used in the study, while the rate of progress from sowing to flowering was influenced by temperature, the rate of progress from flowering to podding was influenced largely by daylength. The practical implications of the results for increased bambara groundnut production are discussed.

Download Full-text

Nitrogen and phosphorus management for Bambara groundnut (Vigna subterranea) production-A review

Legume Research - An International Journal ◽

10.18805/lr-379 ◽

2018 ◽

Author(s):

Mahmudul Hasan ◽

Md Kamal Uddin ◽

Mahmud Tengku Muda Mohamed ◽

Ali Tan Kee Zuan

Keyword(s):

Nitrogen Fixation ◽

Bambara Groundnut ◽

N Fixation ◽

Vigna Subterranea ◽

Nitrogen And Phosphorus ◽

Biotic Stresses ◽

Legume Crop ◽

Biological N Fixation ◽

Calcium Iron ◽

Phosphorus Application

Bambara groundnut (Vigna subterranea) has characteristics to grow in the marginal soil and also tolerant into the drought condition and also have potential of nitrogen fixation. Nitrogen is the key plant nutrient that stimulates root and shoot growth. Phosphorus application significantly improves many aspects of plant physiology including photosynthesis, flowering, fruiting and maturation which ultimately result in better yield. Symbiotic nitrogen fixation is a complex process, in which Rhizobium bacteria form a beneficial interaction with a legume crop to fix atmospheric nitrogen and convert it to ammonium for plant uptake. Biological N fixation (BNF) is the natural means to enhance soil fertility status and productivity. Application of nitrogen and phosphorus fertilizer exerted significant effect on root development, photosynthesis, yield contributing character and pod yield of the crop. Bambara Groundnut yields are low due to abiotic and biotic stresses. But with application of nitrogen and phosphorus increase the yield of this crop. It’s seeds contain 63 percent carbohydrate, 19 percent protein and 6.5 percent oil and good source of fibre, calcium, iron and potassium.

Download Full-text

Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc.) Berdasarkan Marka SSR (Simple Sequence Repeat)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i3.12787 ◽

2017 ◽

Vol 44 (3) ◽

pp. 279

Author(s):

Zikril Illahi ◽

Ni Made Armini Wiendi ◽

Dan Sudarsono

Keyword(s):

Simple Sequence Repeat ◽

Genetic Distances ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Sequence Repeat ◽

Phylogenic Tree ◽

Legume Crop ◽

Population Structure Analysis ◽

Simple Sequence ◽

Underutilized Legume

ABSTRACT Bambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis. Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume

Download Full-text

The complete plastid genomes of two Fabaceae orphan crops from Africa

10.1101/459248 ◽

2018 ◽

Author(s):

Xuezhu Liao ◽

Yang Liu ◽

Huan Liu

Keyword(s):

Plastid Genome ◽

Phylogenetic Analyses ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Lablab Purpureus ◽

Crop Species ◽

Orphan Crops ◽

Plastid Genomes ◽

Orphan Crop ◽

Traditional Crops

AbstractDolichos bean (Lablab purpureus) and Bambara groundnut (Vigna subterranea) are two traditional crops from Africa with important economic values. The study of such neglected or underused crops (orphan crops) should contribute to the resolution of food shortage in Africa. Here we assembled and described the complete plastid genome of the two Fabaceae orphan crop species. The length of the complete plastomes of L. purpureus and V. subterranea are 151,753 bp and 152,015 bp, respectively. Maximum-likelihood (ML) phylogenetic analyses indicated that L. purpureus and Phaseolus vulgaris are closely related, and V. subterranea and other Vigna species are clustered in one clade, which is congruent with former studies.

Download Full-text

Genetic diversity of Bambara groundnut (Vigna subterranea (L.) verdc.) landraces in Kenya using microsatellite markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2014.14082 ◽

2015 ◽

Vol 14 (4) ◽

pp. 283-291 ◽

Cited By ~ 3

Author(s):

O. Odongo Forrester ◽

E.Oyoo Maurice ◽

Wasike Victor ◽

O. Owuoche James ◽

Karanja Laura ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Bambara Groundnut ◽

Vigna Subterranea

Download Full-text

Assessment of genetic diversity and structure of Bambara groundnut [Vigna subterranea (L.) verdc.] landraces in South Africa

Scientific Reports ◽

10.1038/s41598-021-86977-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Adré Minnaar-Ontong ◽

Abe S. Gerrano ◽

Maryke T. Labuschagne

Keyword(s):

South Africa ◽

Genetic Diversity ◽

Ssr Markers ◽

Southern Africa ◽

Crop Improvement ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Legume Crop ◽

Population Structure Analysis ◽

Genetic Diversity And Structure

AbstractWith its drought tolerant and protein-rich properties, Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous African legume crop can contribute immensely to food security. This miracle crop is used as food and for the enhancement of soil fertility in South Africa. Knowledge on the genetic diversity and structure among the Bambara groundnut landraces can pave the way for the effective use and cultivation of this crop in southern Africa, especially South Africa. The aim of this study was to assess the genetic diversity and structure among Bambara groundnut landraces collected across South Africa and compared to a limited number of accessions from southern Africa using SSR markers. Seventy-eight Bambara groundnut accessions were genotyped using 19 Bambara specific SSR markers. SSR loci explored in this study, were all polymorphic. A total of 127 alleles were detected with a mean of 6.7 alleles per locus. Allele diversity and frequency among genotypes varied from 0.21 to 0.85 with an average of 0.62 per locus. Genetic variation as described by the analysis of molecular variance indicated higher genetic diversity (92%) within landraces than between (8%) different landraces. Population structure analysis showed that three subpopulations existed, and most of the South African accessions were restricted to one subpopulation, indicating that Bambara landraces has the ability to form unique haplotypes in different environments. Information harnessed in this study is helpful for further use in breeding programs for crop improvement.

Download Full-text

GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v30i2.35528 ◽

2018 ◽

Vol 30 (2) ◽

pp. 19-28

Author(s):

A. J. Oludare ◽

J. I. Kioko ◽

A. A. Akeem ◽

A. T. Olumide ◽

K. R. Justina ◽

...

Keyword(s):

Genetic Diversity ◽

Seed Proteins ◽

Electrophoretic Analysis ◽

Protein Characterization ◽

Bambara Groundnut ◽

Vigna Subterranea ◽

Phylogenetic Relatedness ◽

Molecular Weights ◽

National Centre ◽

Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

Download Full-text

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽

10.1139/g01-096 ◽

2001 ◽

Vol 44 (6) ◽

pp. 995-999 ◽

Cited By ~ 25

Author(s):

H I Amadou ◽

P J Bebeli ◽

P J Kaltsikes

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Geographic Origin ◽

Bambara Groundnut ◽

International Institute ◽

Tropical Agriculture ◽

Vigna Subterranea ◽

Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

Download Full-text