scholarly journals Isolation of Multi Drug Resistant Escherichia coli from drinking water of Lahore City, Pakistan

2021 ◽  
Vol 15 (5) ◽  
pp. 1080-1083
Author(s):  
Nazia Mir ◽  
Sidrah Saleem ◽  
Muhammad Imran ◽  
Ayesha Ghazal ◽  
Muhammad Usman
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Acinetobacter Species ◽  
E Coli ◽  
Lahore City

Background: The major faecal coliform is Escherichia coli which contaminates the drinking water from human and animal faecal waste. In developing regions drinking water is a vital source of microbiological pathogens. Multi-drug resistant (MDR) is defined as resistance to one or more antibiotic groups. An E. coli count greater than 4/dl in municipal drinking water is indicative of unacceptable fecal contamination. Aim: To find out the frequency of MDR E. coli in water system of Lahore, Pakistan. Methodology: Drinking water was collected from different towns of Lahore. The samples were processed using Membrane Filtration Technique. In the present study, Multidrug Resistant E.coli was isolated and antibiotic resistant pattern was seen against 16 most commonly antimicrobials, using Kirby Bauer disc diffusion method. Results: Total 100 water samples were collected, frequency of Escherichia coliisolated was 27% and frequency of MDR E.coli was 19%. The highest resistance showed by the organism towards Ampicillin (AMP) 21(81.48%), Augmentin (AMC), and Ceftazidime (CAZ) 14(51.85%) respectively. In this study also frequency of Extended Spectrum β-Lactamases were seen. Most common organisms isolated were E. coli 7% and Klebsiella 5%. Frequency of other coliforms isolated from drinking water other than E. coli was Klebsiella species 26%, Pseudomonas species 27%, Enterobacter 7%, Citrobacter species 8% and Acinetobacter species 5%. Conclusion: This study revealed that drinking water of Lahore city is heavilycontaminated with pathogenic microorganisms. A high proportion of antibiotic resistant is due to overuse of antibiotics, in patients with mild infections and secretion of these resistant bacteria from patients to environment. One of the reasons could be the mixing of sewage lines with drinking water supply. So, there is solely requirement for further studies for the identification of the sources for these contaminants. Keywords: Isolates, E. coli, Klebsiella, Multi-drug resistant (MDR)

Isolation of Multi Drug Resistant Escherichia coli from drinking water of Lahore City, Pakistan

2021 ◽  
Vol 15 (6) ◽  
pp. 1188-1191
Author(s):  
N. Mir ◽  
S. Saleem ◽  
M. Imran ◽  
A. Ghazal ◽  
M. Usman
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Acinetobacter Species ◽  
E Coli ◽  
Lahore City

Background: The major faecal coliform is Escherichia coli which contaminates the drinking water from human and animal faecal waste. In developing regions drinking water is a vital source of microbiological pathogens. Multi-drug resistant (MDR) is defined as resistance to one or more antibiotic groups. An E. coli count greater than 4/dl in municipal drinking water is indicative of unacceptable fecal contamination. Aim: To find out the frequency of MDR E. coli in water system of Lahore, Pakistan. Methodology: Drinking water was collected from different towns of Lahore. The samples were processed using Membrane Filtration Technique. In the present study, Multidrug Resistant E.coli was isolated and antibiotic resistant pattern was seen against 16 most commonly antimicrobials, using Kirby Bauer disc diffusion method. Results: Total 100 water samples were collected, frequency of Escherichia coliisolated was 27% and frequency of MDR E.coli was 19%. The highest resistance showed by the organism towards Ampicillin (AMP) 21(81.48%), Augmentin (AMC), and Ceftazidime (CAZ) 14(51.85%) respectively. In this study also frequency of Extended Spectrum β-Lactamases were seen. Most common organisms isolated were E. coli 7% and Klebsiella 5%. Frequency of other coliforms isolated from drinking water other than E. coli was Klebsiella species 26%, Pseudomonas species 27%, Enterobacter 7%, Citrobacter species 8% and Acinetobacter species 5%. Conclusion: This study revealed that drinking water of Lahore city is heavilycontaminated with pathogenic microorganisms. A high proportion of antibiotic resistant is due to overuse of antibiotics, in patients with mild infections and secretion of these resistant bacteria from patients to environment. One of the reasons could be the mixing of sewage lines with drinking water supply. So, there is solely requirement for further studies for the identification of the sources for these contaminants. Keywords: Isolates, E. coli, Klebsiella, Multi-drug resistant (MDR)

scholarly journals Untreated Liquid Hospital Waste: Potential Source of Multidrug Resistant Bacteria

2016 ◽  
pp. 21-24
Author(s):  
Md Kamruzzaman Siddiqui ◽  
Nazma Khatoon ◽  
Pravas Chandra Roy
Keyword(s):  
Antimicrobial Resistance ◽  
Waste Water Treatment Plant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Antimicrobial Resistance Genes

Antimicrobial resistance in both pathogenic and commensal bacteria is increasing steadily. Failure of antibiotic resistant bacteria containment is responsible for this expansion. Healthcare effluent acts as the store house of harmful infectious pathogens. Potential health risk includes spreading of diseases by these pathogens and wide dissemination of antimicrobial resistance genes. The present study was carried out to investigate the multiple-drug resistance among the bacterial strains that were isolated and identified from the effluents of Jessore Medical College Hospital & Jessore Queen’s hospital private limited. Identified bacteria were E. coli , Klebsiella spp., Enterobacter spp., Proteus vulgaris and Salmonella spp.. Occurrence of E. coli and Enterobacter spp. were found to have the highest percentages and present in majority of the samples. The identified organisms antibiotic resistant pattern were analyzed by agar disc diffusion method against 6 antibiotics. Results of antibiotic susceptibility test showed that all of the isolates were multi-drug resistant (e”4). From the study, we observed that 75% of the isolates were resistant to amoxicillin, followed by Ampicillin (64%), Chloramphenicol (31%), Gentamycin (29%), Nitrofurantoin (27%) and least resistant being Ciprofloxacin 23%. Among the isolates Salmonella spp. were showed highest rate of resistance against all the used antibiotics. The result denotes that, the identified bacteria have been well exposed to the tested antimicrobials and they have established mechanisms to avoid them. Therefore, proper waste water treatment plant should be established to diminish the risk of disseminating multiple drug resistant microorganisms for the safeguard of public health.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 21-24

scholarly journals Antimicrobial Resistance, Virulence Factors, and Pathotypes of Escherichia coli Isolated from Drinking Water Sources in Jordan

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 86 ◽  
Author(s):  
Samer Swedan ◽  
Heba Abu Alrub
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Sources ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Disk Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Drinking Water Sources ◽  
Synergy Test

The study investigated the prevalence of potentially pathogenic and drug resistant Escherichia coli among drinking water sources in Jordan. A total of 109 confirmed E. coli isolates were analyzed. Antimicrobial susceptibility testing was done using the Kirby Bauer disk diffusion method. Phenotypic identification of extended spectrum beta-lactamase (ESBL) and carbapenemase production was done using the double disk synergy test and the modified Hodge test, respectively. Isolates’ plasmid profiles were determined by gel electrophoresis. PCR was used for detection of virulence and resistance genes. Overall, 22.0% of the isolates were potentially intestinal pathogenic E. coli (IPEC); namely enteroaggregative E. coli (16.5%), enteropathogenic E. coli (2.8%), enteroinvasive E. coli (1.8%), and enterohemorrhagic E. coli (0.9%). A third of the isolates were multi-drug resistant. The highest rates of antimicrobials resistance were observed against ampicillin (93.6%) and sulfamethoxazole/trimethoprim (41.3%). All isolates were susceptible to imipenem, meropenem, doripenem and tigecycline. The prevalence of ESBL and carbapenemase producers was 54.1% and 2.8%, respectively. BlaVIM was the most prevalent resistance gene (68.8%), followed by blaCTX (50.5%), blaTEM (45.9%), blaNDM (11%), blaKPC (4.6%), and blaSHV (0.9%). Fifty-eight (53.2%) isolates contained one or more plasmid ranging from 1.0 to 8.0 kbp. Overall, high prevalence of potentially pathogenic and resistant isolates was observed.

scholarly journals Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from hospital wastewater

2018 ◽  
Vol 37 (3) ◽  
pp. 209 ◽  
Author(s):  
Reza Ranjbar ◽  
Maryam Zeynali ◽  
Nooshin Sohrabi ◽  
Asghar Ali Kamboh
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Hospital Wastewater ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Class 1 ◽  
Hospital Wastewaters

BACKGROUND <br />Hospital wastewaters may contain antibiotic resistant bacteria such as Escherichia coli. These E. coli harbor integron genes that are responsible for antibiotic resistance. The purpose of the current study was to evaluate the frequency of class 1 and 2 integrons in environmental antibiotic resistant E. coli strains isolated from the hospital wastewaters in Tehran, Iran.<br /><br />METHODS<br />As a descriptive cross-sectional study, this research was performed from April to September 2015 on hospital wastewaters in Tehran. Bacterial isolation and identification was performed by standard biochemical and bacteriological procedures. Susceptibility testing was done by employing the disk diffusion method using different antibiotics. Total DNAs were extracted to evaluate the presence of class 1 and 2 integrons by using the polymerase chain reaction (PCR) method with specific primers. <br /><br />RESULTS<br />Fifty E. coli strains were isolated and identified from the wastewaters of 25 hospitals in Tehran. The phenotype results showed that 46 isolates (92%) were resistant to at least one antibiotic and 27 isolates (54%) were multidrug resistant. PCR showed that 35 (70%) and 20 (40%) of the isolates had class 1 and 2 integrons respectively and 14 isolates (28%) had both class 1 and class 2 integrons.<br /><br />CONCLUSION<br />This study has shown a considerable presence of class 1 and class 2 integrons in E. coli strains isolated from hospital wastewaters in Tehran. Proper antibiotics prescription and appropriate hospital wastewater treatment can prevent resistance genes in E. coli from circulating in the environment.

scholarly journals Fate and seasonal change of Escherichia coli resistant to different antibiotic classes at each stage of conventional activated sludge process

2020 ◽  
Vol 18 (6) ◽  
pp. 879-889 ◽  
Author(s):  
Ryo Honda ◽  
Chihiro Tachi ◽  
Mana Noguchi ◽  
Ryoko Yamamoto-Ikemoto ◽  
Toru Watanabe
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Activated Sludge ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Secondary Treatment ◽  
Activated Sludge Process ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Influent Wastewater

Abstract This study investigated the impact of each treatment stage of the activated sludge process on the fate of antibiotic resistant bacteria (ARB) in wastewater treatment plants (WWTPs). Wastewater and sludge samples were collected monthly at each stage of a commercial-scale WWTP. After 20–25 strains of indicator Escherichia coli were isolated from each sample on Chromocult Coliform Agar, antibiotic resistance of the isolates to amoxicillin (AMX), ciprofloxacin (CIP), norfloxacin (NFX), kanamycin (KM), sulfamethoxazole/trimethoprim (ST) and tetracycline (TC) were tested with the Kirby–Bauer disk diffusion method. As a result, activated sludge in the aeration tank and return sludge had higher abundance of antibiotic resistant E. coli than influent wastewater and secondary treatment effluent. AMX resistant E. coli was enriched in return sludge at the secondary clarifier. Higher temperature was also likely to cause an increase of AMX resistant E. coli in sludge. The antibiotic resistance profile of E. coli in secondary treatment effluent was more dependent on activated sludge than influent wastewater. These results suggested that activated sludge in WWTP possibly serves as a reservoir of ARB, and that behavior of ARB in WWTP differs by antibiotic classes.

scholarly journals Tetracycline resistance in enterococci and Escherichia coli isolated from fresh produce and why it matters

2021 ◽  
Vol 10 (2) ◽  
pp. 359-370
Author(s):  
Zahra S. Al-Kharousi ◽  
Nejib Guizani ◽  
Abdullah M. Al-Sadi ◽  
Ismail M. Al-Bulushi
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Fresh Produce ◽  
Tetracycline Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Market Place ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli

The contamination of fresh produce with antibiotic-resistant bacteria is of particular concern as they are often eaten raw and can be a source for foodborne diseases. Tetracyclines have been largely used in humans, animals and plants which might have accelerated microbial resistance to them. Enterococci and Escherichia coli can be used as indicators to monitor contamination of the fresh produce with tetracycline-resistant bacteria. The investigation related to this issue is very scarce in Oman. This study aimed at identifying tetracycline-resistant enterococci and E. coli in fresh produce at the market place. Thirty-one enterococci and ten E. coli were isolated from local (Oman) and imported fruits and vegetables (N= 105). Using the standard Kirby-Bauer disc diffusion method, resistance to tetracycline was found in 6 (19 %) enterococci, isolated from cucumber, lettuce and radish, and 5 (50 %) E. coli, obtained from cabbage, lettuce and radish. Genetic analysis revealed the presence of tetracycline resistance genes, tet(A) and tet(K), in E. coli and tet(K), tet(L) and tet(M) in enterococci, including Enterococcus sulfureus, Enterococcus mundtii, Enterococcus casseli avus and Enterococcus faecalis. The integron integrase IntI 1 gene, which is known to facilitate the dissemination of antibiotic resistance genes among bacteria, was detected in 2 isolates of E. coli. These results demonstrated the capability of fresh produce to act as a potential source for disseminating tetracycline or possibly other antibiotic-resistant bacteria through the food chain. Thus, control strategies are needed to reduce exposure of the public to such microorganisms.

scholarly journals Molecular detection of beta-lactamase blaCTX-M group 1 in Escherichia coli isolated from drinking water in Khartoum State

2020 ◽  
Vol 18 (6) ◽  
pp. 1091-1097
Author(s):  
Hisham N. Altayb ◽  
Eman Khalid Salih ◽  
Ehssan H. Moglad
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Culture Media ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Group 1

Abstract This study aimed to detect the blaCTX-M group 1 in Escherichia coli (E. coli) isolated from drinking water in Khartoum State. Two hundred and eighty water samples were collected randomly from different areas, places, and sources from the state and examined for the presence of E. coli as a fecal contamination indicator. Isolation and identification of E. coli were performed using culture characteristics on different culture media and biochemical reactions. An antimicrobial sensitivity test was performed for all isolated E. coli using agar disk diffusion method. DNA was extracted by boiling method, and bacterial genomic DNA used as a template to detect blaCTX-M group 1 by PCR. Results showed 86 (30.7%) E. coli were isolated out of 280 water samples. Antimicrobial susceptibility testing revealed the highest resistant percentage was 59% for tetracycline, followed by 35% for gentamycin, while for chloramphenicol and cefotaxime was 22 and 20%, respectively. blaCTX-M group 1 was detected in about 40% of all isolates. This study concludes that drinking water in Khartoum State may be contaminated with feces and might be a possible source for transferring resistant bacteria. Thus, it may be one of the critical causes of increasing reports of antimicrobial resistance in Khartoum State.

scholarly journals Molecular detection of Beta-lactamase blaCTX-M group 1 gene in Escherichia coli Isolated from Drinking Water in Khartoum State

2020 ◽  
Author(s):  
Hisham N Altayeb ◽  
Eman Khalid ◽  
Ehssan h Moglad
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Current Evidence ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Isolation And Identification ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Group 1

Abstract BackgroundOne of the most common challenges facing the world today is the rapid spread of antibiotic-resistant bacteria. According to current evidence, this is mainly as a result of presumed use of antibiotics, the interaction between humans or from animal sources. Still, there is limited focus on the role of environmental factors, especially water. This study aimed to detect the blaCTX-M group 1 gene in Escherichia coli (E. coli) isolated from drinking water in Khartoum State. Methods Two hundred and eighty water samples were collected from different localities in Khartoum State and resources. Isolation and identification of E. coli performed by using lauryl tryptose broth, Brilliant Green Bile Broth, biochemical reaction, and culture characteristics on EMB media. Antimicrobial sensitivity tests were performed for all isolated bacteria using cefotaxime (30μg), gentamycin (10μg), tetracycline (25μg) and chloramphenicol (30μg) by agar diffusion method. DNA extracted by boiling method, bacterial genomic DNA used as a template to detect the blaCTX-M group 1 gene by PCR. Results The result displayed that eighty-six (30.7%) E. coli isolated out of two hundred and eighty water samples. Out of eighty-sixing isolated bacteria, 49% (42/86) were from Khartoum locality, 28% (24/86) were from Omdurman locality, and 23% (20/86) were from Khartoum North locality. Antimicrobial susceptibility testing revealed that the highest resistant percentage was 59% for tetracycline, followed by 35% for gentamycin. While for chloramphenicol and cefotaxime were 22% and 20%, respectively. CTX-M group 1 gene was detected in 40% of all isolates. Conclusion This study concludes that drinking water in Khartoum State might be contaminated with faces and be a possible source for the transmission of resistant bacteria to humans.

scholarly journals Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing Antibiotic-Resistant Escherichia coli and Klebsiella pneumoniae in Ready-to-Eat Street Foods

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 850
Author(s):  
Shobha Giri ◽  
Vaishnavi Kudva ◽  
Kalidas Shetty ◽  
Veena Shetty
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Klebsiella Pneumoniae ◽  
Rural Areas ◽  
Significant Risk ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Extended Spectrum

As the global urban populations increase with rapid migration from rural areas, ready-to-eat (RTE) street foods are posing food safety challenges where street foods are prepared with less structured food safety guidelines in small and roadside outlets. The increased presence of extended-spectrum-β-lactamase (ESBL) producing bacteria in street foods is a significant risk for human health because of its epidemiological significance. Escherichia coli and Klebsiella pneumoniae have become important and dangerous foodborne pathogens globally for their relevance to antibiotic resistance. The present study was undertaken to evaluate the potential burden of antibiotic-resistant E. coli and K. pneumoniae contaminating RTE street foods and to assess the microbiological quality of foods in a typical emerging and growing urban suburb of India where RTE street foods are rapidly establishing with public health implications. A total of 100 RTE food samples were collected of which, 22.88% were E. coli and 27.12% K. pneumoniae. The prevalence of ESBL-producing E. coli and K. pneumoniae was 25.42%, isolated mostly from chutneys, salads, paani puri, and chicken. Antimicrobial resistance was observed towards cefepime (72.9%), imipenem (55.9%), cefotaxime (52.5%), and meropenem (16.9%) with 86.44% of the isolates with MAR index above 0.22. Among β-lactamase encoding genes, blaTEM (40.68%) was the most prevalent followed by blaCTX (32.20%) and blaSHV (10.17%). blaNDM gene was detected in 20.34% of the isolates. This study indicated that contaminated RTE street foods present health risks to consumers and there is a high potential of transferring multi-drug-resistant bacteria from foods to humans and from person to person as pathogens or as commensal residents of the human gut leading to challenges for subsequent therapeutic treatments.

scholarly journals Phenotypic Antimicrobial Susceptibility of Escherichia coli from Raw Meats, Ready-to-Eat Meats, and Their Related Samples in One Health Context

2021 ◽  
Vol 9 (2) ◽  
pp. 326
Author(s):  
Frederick Adzitey ◽  
Nurul Huda ◽  
Amir Husni Mohd Shariff
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Resistance Index ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Disk Diffusion Method ◽  
E Coli ◽  
The Usa ◽  
Raw Meats

Meat is an important food source that can provide a significant amount of protein for human development. The occurrence of bacteria that are resistant to antimicrobials in meat poses a public health risk. This study evaluated the occurrence and antimicrobial resistance of E. coli (Escherichia coli) isolated from raw meats, ready-to-eat (RTE) meats and their related samples in Ghana. E. coli was isolated using the USA-FDA Bacteriological Analytical Manual and phenotypic antimicrobial susceptibility test was performed by the disk diffusion method. Of the 200 examined meats and their related samples, 38% were positive for E. coli. Notably, E. coli was highest in raw beef (80%) and lowest in RTE pork (0%). The 45 E. coli isolates were resistant ≥ 50% to amoxicillin, trimethoprim and tetracycline. They were susceptible to azithromycin (87.1%), chloramphenicol (81.3%), imipenem (74.8%), gentamicin (72.0%) and ciprofloxacin (69.5%). A relatively high intermediate resistance of 33.0% was observed for ceftriaxone. E. coli from raw meats, RTE meats, hands of meat sellers and working tools showed some differences and similarities in their phenotypic antimicrobial resistance patterns. Half (51.1%) of the E. coli isolates exhibited multidrug resistance. The E. coli isolates showed twenty-two different resistant patterns, with a multiple antibiotic resistance index of 0.0 to 0.7. The resistant pattern amoxicillin (A, n = 6 isolates) and amoxicillin-trimethoprim (A-TM, n = 6 isolates) were the most common. This study documents that raw meats, RTE meats and their related samples in Ghana are potential sources of antimicrobial-resistant E. coli and pose a risk for the transfer of resistant bacteria to the food chain, environment and humans.

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