scholarly journals Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology

2007 ◽  
Vol 45 (1) ◽  
pp. 42-46 ◽  
Author(s):  
G. Szita ◽  
M. Gyenes ◽  
L. Soós ◽  
T. Rétfalvi ◽  
L. Békési ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Synthetic Medium

scholarly journals Microbial Diversity in Water and Biofilm Samples from Well Sources in Ilorin Metropolis, Nigeria

2019 ◽  
Vol 11 (1) ◽  
pp. 56-62
Author(s):  
Toyin Olayemi AGBABIAKA ◽  
Blessing Olubunmi OLOFINTOYE
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Salmonella Enteritidis ◽  
Micrococcus Luteus ◽  
Bacillus Sphaericus ◽  
Shigella Dysenteriae ◽  
Total Bacterial Count ◽  
Bacillus Pasteurii ◽  
Potential Health ◽  
Temperature Conductivity

The aim of the study was to investigate the microbial populations of water and biofilm samples from seven hand dug wells in Ilorin, Nigeria, which are for private and public use. Physicochemical parameters such as pH, turbidity, total dissolved solids, temperature, conductivity, dissolved oxygen and biological oxygen demand were determined. Biofilm growths were induced from water samples obtained from selected wells. Twenty bacteria isolated were identified using morphological and biochemical characteristics as well as molecular methods and includes Shigella sonnei, Shigella dysenteriae, Micrococcus luteus, Bacillus sphaericus, Salmonella enteritidis, Proteus mirabilis strain IK-MB4-518F, Bacillus licheniformis strain RH104, Bacillus subtilis, Erwinia sp., Proteus vulgaris, Yersina sp., Bacillus cereus, Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa strain GS1, Pseudomonas aeruginosa strain 218B, Staphylococcus epidermidis, Vibrio sp., Escherichia coli and Bacillus pasteurii. Total bacterial count ranged from 1.8×104-1.53×106 cfu/ml. Water samples from these wells are therefore not potable and some of the isolates are potential health hazards to human population.

Commercialization Conditions and Practices Influence the Microbiological Quality of Mineral Waters

2008 ◽  
Vol 71 (6) ◽  
pp. 1253-1257 ◽  
Author(s):  
SÉRGIO A. P. NUNES FILHO ◽  
ANDERSON S. SANT'ANA ◽  
ADRIANO G. CRUZ
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Mineral Water ◽  
Microbiological Quality ◽  
Mineral Waters ◽  
Fecal Coliforms ◽  
Street Vendors ◽  
Total Coliforms ◽  
Bottled Mineral Water

The objective of the present study was to determine the microbiological quality of bottled mineral water marketed in commercial establishments and by street vendors and to evaluate the influence of the storage and maintenance conditions on the microbiological quality of the product. Ten samples from the same batches of five different brands of water were analyzed, for a total of 50 samples. Of the five brands analyzed, only one (brand A), when collected in a commercial establishment, complied with the legal Brazilian standards for mineral water with respect to the presence of total coliforms, fecal coliforms, and Pseudomonas aeruginosa. The remaining samples failed to comply with these microbiological standards for at least one of the parameters evaluated. The water samples obtained from street vendors were inferior in microbiological quality to samples from the same batch that were obtained from commercial establishments.

scholarly journals ISOLAMENTO DE MICRORGANISMOS DEGRADADORES DE COMPOSTOS LIPÍDICOS DE ORIGEM VEGETAL EM AMOSTRAS DE ÁGUAS DA BARRAGEM DO RIO PASSAÚNA-ARAUCÁRIA, PR

2003 ◽  
Vol 21 (1) ◽  
Author(s):  
JUÇARA FEITOSA ◽  
IDA CHAPAVAL PIMENTEL ◽  
MÁRCIA REGINA BEUX ◽  
CARLOS ROBERTO DALKE ◽  
FABIANA ZARA PASTRO ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Culture Media ◽  
Soy Oil ◽  
Selection Of

Avaliou-se o potencial de degradação de compostos lipídicos, naturalmente presentes em amostras de água da Barragem do Rio Passaúna (Curitiba-Paraná/Brasil) ou artificialmente incorporados a meios de cultura, por cepas de Pseudomonas aeruginosa e por microrganismos nãoselecionados. Os resultados demonstraram degradação do substrato lipídico (óleo de soja virgem e utilizado) apenas em relação ao experimento em que não houve o isolamento e seleção prévia de cepas específicas. ISOLATION OF MICROORGANISMS THAT DEGRADE LIPIDIC COMPOUNDS OF VEGETAL ORIGIN IN WATER SAMPLES OF PASSAÚNA RIVER DAM – ARAUCÁRIA-PR (BRAZIL) Abstract It was evaluated the degradation potential of lipidic compounds naturally present in water samples of Passaúna River dam (Curitiba – Paraná/Brazil) or artificially incorporated to culture media by strains of Pseudomonas aeruginosa and by non-selected microorganisms. The results demonstrated degradation of the lipidic substrate (virgin soy oil and already used) only in relation to the experiment where no isolation and previous selection of specific strains was made.

scholarly journals A study on metallo--lactamases producing Pseudomonas aeruginosa in water samples from various parts of Malaysia

2017 ◽  
Vol 16 (12) ◽  
pp. 573-584
Author(s):  
Suryadevara Nagaraja ◽  
Yi Shuang Ooi ◽  
Ponniaih Paulraj
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples

scholarly journals Contamination of Hospital Water Supplies in Gilan, Iran, withLegionella pneumophila, Escherichia coli,andPseudomonas aeruginosa

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Masoumeh Ahmadi Jalali Moghadam ◽  
Hamidreza Honarmand ◽  
Sajad Asfaram Meshginshahr
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Bacterial Contamination ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Water Supplies ◽  
E Coli ◽  
Contamination Degree ◽  
Commercial Kit

This study is designed to determine the contamination degree of hospital water supplies withPseudomonas aeruginosa, Legionella pneumophila, andE. coliin Gilan, Iran. Samples were collected directly into sterile containers and concentrated by centrifuge. Half part of any sample transferred to yeast extract broth and the second part transferred to Trypticase Soy Broth and incubated for 3 days. DNA was extracted by using commercial kit. Four rounds of PCR were performed as follows: multiplex PCR for detectingPseudomonas aeruginosa, Integron 1, and Metallo-β-lactamases gene; PCR for detectingLegionella pneumophilaandmipgene separately; PCR for detectingE. coli; and another PCR for detecting whole bacterial presence. Contamination rates of cold, warm, and incubator water samples withP. aeruginosa, were 16.6%, 37.5%, and 6.8% consequently. Degrees of contamination withL. pneumophilawere 3.3%, 9.3%, and 10.9% and withE. coliwere zero, 6.2%, and zero. Total bacterial contamination of cold, warm, and incubator water samples was 93.3%, 84.4%, and 89.0% consequently. Metallo-β-lactamases gene was found in 20.0% of all samples. Contamination degree withP. aeruginosawas considerable and withL. pneumophilawas moderate. Metallo-β-lactamases gene was found frequently indicating widespread multiple drug resistance bacteria. We suggest using new decontamination method based on nanotechnology.

scholarly journals Macroporous epoxy-based monoliths for rapid quantification of Pseudomonas aeruginosa by adsorption elution method optimized for qPCR

2020 ◽  
Vol 412 (29) ◽  
pp. 8185-8195
Author(s):  
Lisa Göpfert ◽  
Julia Klüpfel ◽  
Charlotte Heinritz ◽  
Martin Elsner ◽  
Michael Seidel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Tap Water ◽  
Cell Number ◽  
Sample Volume ◽  
Detection Methods ◽  
Culture Independent ◽  
Elution Method ◽  
Optimized Conditions

Abstract Pseudomonas aeruginosa contaminations in tap water systems have caused severe health problems in both hospital and household settings. To ensure fast and reliable detection, culture-independent methods are recommendable. However, the typically low cell number in water samples requires sample enrichment prior to analysis. Therefore, we developed and optimized an adsorption elution method using monolithic adsorption filtration and subsequent centrifugal ultrafiltration that can be combined with culture-independent detection methods. The principle of adsorption of Pseudomonas aeruginosa by hydrophobic and ionic interactions was studied in modified epoxy-based monoliths. Optimized conditions (5-L initial sample volume at pH 3 filtered for 30 min through hydrolyzed monoliths (MAF-OH) and eluted with beef extract glycine buffer at pH 9.5) achieved a recovery of 67.1 ± 1.2% and a concentration factor of 103. For the first time, we therefore present a culture-independent approach for rapid enrichment and subsequent molecular biological quantification of P. aeruginosa by qPCR from tap water samples by monolithic adsorption filtration. The total enrichment and quantification process takes 4 h. This work further stresses the versatility of the monolithic adsorption filtration and its possibilities as a concentration tool for culture-independent analytics of pathogenic bacteria in the environment. Graphical abstract

scholarly journals Antibiotic susceptibility pattern of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from some drinking wells in Ondo town southwest Nigeria

2021 ◽  
Vol 25 (1) ◽  
pp. 59-63
Author(s):  
O. Aromolaran ◽  
O.A. Amodu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Well Water ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Southwest Nigeria

Antibiotic-resistant bacteria (ARB) strains have become a global health threat. This study aimed to determine the antibiotic susceptibility pattern of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from wells in Ondo town, Southwest Nigeria. Twenty-eight well water samples were analyzed for the presence of K. pneumoniae and P. aeruginosa by standard pour plate technique. The bacterial isolates were tested against eight commonly use antibiotics using Kirby Bauer disc diffusion method. The percentage occurrence of K. pneumoniae and P. aeruginosa in the well water samples were 17.86% and 21.43%, respectively. Two multi-drug resistant strains of K. pneumoniae were isolated, which were resistant to at least three classes of antibiotics. Fifty percent of the P. aeruginosa isolates were resistant to caftazidime, cefuroxime, nitrofurantoin, and ampicillin. None of the isolates was fully susceptible to cefuroxime, but have all showed resistance to β-lactam (ceftazidime, cefuroxime augmentin, and ampicillin) antibiotics. Cefuroxime may not be effective an effective drug in the treatment of K. pneumoniae and P. aeruginosa implicated infections in these communities in Ondo. Also, the over-use of antibiotics should be discouraged in order to curtail the menace of antibiotic resistance Keywords: Well water, Antibiotics, Klebsiella pneumoniae, Pseudomonas aeruginosa

scholarly journals Phenotypical Comparison of Pseudomonas Aeruginosa Isolated from Human and Veterinary Samples; Impact of Host Adaptation on Infection Pathogenesis

2021 ◽  
Author(s):  
Stefan J. Kaiser ◽  
Annalisa DeRosa ◽  
Christa Ewers ◽  
Frank Günther
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Biofilm Formation ◽  
Polymorphonuclear Leukocytes ◽  
Environmental Isolates ◽  
Antibiotic Resistant ◽  
Pet Owners ◽  
Zoonotic Risk ◽  
Genetically Determined

Abstract Purpose: Determinants of virulence in Pseudomonas aeruginosa vary strongly depending on its habitat. In this study, we analyzed these alterations depending on the host organism in isolates cultured from canine ears and compared it to clinical extended-spectrum antibiotic-resistant Pseudomonas aeruginosa isolates (XDR), clinical antibiotic-sensitive (non-XDR) from humans and environmental isolates (EI) analyzed during our first study in 2017. Methods: A total of 22 veterinary isolates cultured from canine ears (VET) were examined for spontaneous biofilm formation, stress response in biofilm formation induced by meropenem, in vitro fitness, susceptibility to human serum and polymorphonuclear leukocytes and the genetically determined virulence factors toxA, exoS, exoT, exoU, exoY, nan1, cif, lasA and lasB.Results: We observed significantly elevated spontaneous biofilm formation and serum susceptibility in VET isolates compared to EI and non-XDR isolates as well as significantly decreased in vitro fitness compared to XDR isolates. The VET isolates resembled most the XDR subgroup of isolates previously cultured from blood. Within the environmental isolates, we observed an increase of spontaneous biofilm formation and exoU presence in isolates cultured from community water samples over hospital water samples to pool samples.Conclusions: Considering the distinct differences in some features of the examined VET isolates, a higher degree of phenotypical adaption can be assumed. Increased biofilm formation seems to be a common and characteristic event in isolates adapted to a specific habitat. Therefore amplification of potentially more virulent Pseudomonas aeruginosa strains in domestic animals may lead to elevated zoonotic risk for example for pet owners.

scholarly journals PERSISTENCE OF TOTAL AND FECAL COLIFORMS AND Pseudomonas aeruginosa IN IN NATURA WATER SAMPLES FROM RIVERS THAT SUPPLY A WATER TREATMENT STATION IN CURITIBA, BRAZIL

2008 ◽  
Vol 30 (70/72) ◽  
Author(s):  
Mônica Paul Freitas ◽  
Marita Maciel Moreira Laskowski ◽  
Carlos Roberto Dalke ◽  
Patricia do Rocio Dalzoto ◽  
Ida Chapaval Pimentel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Membrane Filtration ◽  
Survival Rates ◽  
Enteric Bacteria ◽  
Fecal Coliforms ◽  
Filtration Method ◽  
Total Coliforms ◽  
Water Treatment Station ◽  
Iguaçu River

An unknown parcel of the planet water is contaminated by chemical and/or biological agents. Human and other animals excreta have been associated to many infectious diseases that can be disseminated through contaminated water. Enteric bacteria and other pathogens can cause gastroenteritis, cholera, systemic infections, among others. Once in the environment, these microorganisms can persist for large periods of time and under certain conditions can even replicate, increasing their number. This paper aimed the research and evaluation of survival rates for total coliforms, fecal coliforms (Escherichia coli) and Pseudomonas aeruginosa in natura water samples from Passaúna and Iraí barrages and Iguaçú River, in Curitiba, Paraná. Colimetric analysis of water samples were performed by the membrane filtration method 64 Freitas MP, Blaskowski MMM, Dalke CR, Dalzoto PR, Pimentel IC. Estud and P. aeruginosa research was assayed using the multiple tubes method in Asparagin and Acetamyde Broth. Confirmatory tests such as catalase presence, citocrome oxidase test and pigment P (pyocyanin) production were also performed. It was observed the occurrence of total coliforms in water samples from Iguaçú River for approximately 50 days, while in Iraí and Passaúna barrages, the periods were 35 and 14 days, respectively. Fecal coliforms were found for about 35 days in samples from Iguaçú River and Iraí barrage, while in Passaúna barrage only for 14 days. P. aeruginosa persisted for approximately 63 days in all the water samples assayed. The higher persistence rate observed in P. aeruginosa can be related to an antagonism towards total and fecal coliforms, which had their populations decreased.

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