scholarly journals Microbial Diversity in Water and Biofilm Samples from Well Sources in Ilorin Metropolis, Nigeria

2019 ◽  
Vol 11 (1) ◽  
pp. 56-62
Author(s):  
Toyin Olayemi AGBABIAKA ◽  
Blessing Olubunmi OLOFINTOYE
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Salmonella Enteritidis ◽  
Micrococcus Luteus ◽  
Bacillus Sphaericus ◽  
Shigella Dysenteriae ◽  
Total Bacterial Count ◽  
Bacillus Pasteurii ◽  
Potential Health ◽  
Temperature Conductivity

The aim of the study was to investigate the microbial populations of water and biofilm samples from seven hand dug wells in Ilorin, Nigeria, which are for private and public use. Physicochemical parameters such as pH, turbidity, total dissolved solids, temperature, conductivity, dissolved oxygen and biological oxygen demand were determined. Biofilm growths were induced from water samples obtained from selected wells. Twenty bacteria isolated were identified using morphological and biochemical characteristics as well as molecular methods and includes Shigella sonnei, Shigella dysenteriae, Micrococcus luteus, Bacillus sphaericus, Salmonella enteritidis, Proteus mirabilis strain IK-MB4-518F, Bacillus licheniformis strain RH104, Bacillus subtilis, Erwinia sp., Proteus vulgaris, Yersina sp., Bacillus cereus, Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa strain GS1, Pseudomonas aeruginosa strain 218B, Staphylococcus epidermidis, Vibrio sp., Escherichia coli and Bacillus pasteurii. Total bacterial count ranged from 1.8×104-1.53×106 cfu/ml. Water samples from these wells are therefore not potable and some of the isolates are potential health hazards to human population.

Antibiotic sensitivity of bacteria associated with selected waste dumpsites in Akure, Nigeria

2020 ◽  
Vol 15 (1) ◽  
pp. 28-31
Author(s):  
Olalemi Adewale ◽  
Arotupin Daniel ◽  
Babalola Funmilayo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Waste Management ◽  
Environmental Health ◽  
Management Practices ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Shigella Dysenteriae ◽  
Plate Count ◽  
Sensitivity Pattern

A study was carried out to determine the antibiotic sensitivity pattern of bacteria isolates associated with selected waste dumpsites in Akure metropolis, Nigeria. Results revealed that the total plate count of bacteria ranged from 1.3×105 cfu/g to 3.0×106 cfu/g. Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi, Streptococcus faecalis, Shigella dysenteriae, Escherichia coli, Micrococcus luteus, Serratia marcescens, Clostridium botulinum were the isolated bacterial species. The temperature and pH values ranged from 26˚C to 28˚C and 5.68 to 6.48 respectively. Pseudomonas aeruginosa and Serratia showed susceptibility to antibiotics while Micrococcus luteus exhibited resistance to the antibiotics. Some of these isolated bacteria are pathogenic and thus, poses potential threat to the local inhabitant of the area. Therefore, proper waste management practices must be put in place to avoid environmental health hazards. Keywords: bacteria, dumpsites, antibiotic, waste management, environmental health

Occurrences of Giardia cysts in beach water

1998 ◽  
Vol 38 (12) ◽  
pp. 73-76 ◽  
Author(s):  
B. S. W. Ho ◽  
T.-Y Tam
Keyword(s):  
Health Risk ◽  
Water Sample ◽  
Water Samples ◽  
High Concentration ◽  
Potential Health ◽  
Infectious Dose ◽  
Potential Health Risk ◽  
E Coli ◽  
Beach Water ◽  
Giardia Cysts

A total of 64 beach water samples with various bacteriological quality (Grades 1 to 4) were analysed for their bacteriological and parasitological contents (E coli and Giardia cysts respectively). Results indicated that Giardia cysts were detected in less than 10% of the Grade 1 beach water samples with E coli concentrations of <24/100mL. For Grades 2, 3 & 4 beach water samples, Giardia cysts were found, respectively, in 85, 50 and 64% of the samples. Except for one beach water sample which had an unusually high concentration of Giardia cysts (23 cysts/L), they were generally present at moderate concentrations (<10 cysts/L) in all other beach water samples. Despite moderate levels of Giardia cysts present in beach water of different grades, the potential health risk faced by swimmers bathing in local beach water needs to be carefully assessed as Giardia is known to have a low infectious dose.

scholarly journals Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology

2007 ◽  
Vol 45 (1) ◽  
pp. 42-46 ◽  
Author(s):  
G. Szita ◽  
M. Gyenes ◽  
L. Soós ◽  
T. Rétfalvi ◽  
L. Békési ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Synthetic Medium

Growth of Salmonella Enteritidis in the presence of quorum sensing signaling compounds produced by Pseudomonas aeruginosa

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Weijia He ◽  
Huamei Yang ◽  
Xiang Wang ◽  
Hongmei Li ◽  
Qingli Dong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Salmonella Enteritidis ◽  
Culture Supernatant ◽  
Lag Time ◽  
Bacterial Communication ◽  
Homoserine Lactones ◽  
Positive Effects ◽  
Kinetics Of

Abstract Quorum sensing (QS) can exist in food-related bacteria and potentially affect bacterial growth through acyl-homoserine lactones (AHLs). To verify the role of QS compounds in the cell-free supernatant, this study examined the effect of supernatant extracted from Pseudomonas aeruginosa culture on the growth kinetics of Salmonella Enteritidis. The results showed that the lag time (λ) of S. Enteritidis was apparently reduced (p < 0.05) under the influence of P. aeruginosa culture supernatant compared with the S. Enteritidis culture supernatant. HPLC-MS/MS test demonstrated that AHLs secreted by P. aeruginosa were mainly C14-HSL with a content of 85.71 μg/mL and a small amount of 3-oxo-C12-HSL. In addition, the commercially synthetic C14-HSL had positive effects on the growth of S. Enteritidis, confirming once again that the growth of S. Enteritidis was affected by AHL metabolized by other bacteria and the complexity of bacterial communication.

scholarly journals Yeasts and filamentous fungi in bottled mineral water and tap water from municipal supplies

2007 ◽  
Vol 50 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mirian Ueda Yamaguchi ◽  
Rita de Cássia Pontello Rampazzo ◽  
Sueli Fumie Yamada-Ogatta ◽  
Celso Vataru Nakamura ◽  
Tânia Ueda-Nakamura ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Water Samples ◽  
Mineral Water ◽  
Tap Water ◽  
Health Hazard ◽  
Total Coliform ◽  
Species Level ◽  
Potential Health ◽  
Potential Health Hazard ◽  
Bottled Mineral Water

The main objective of this study was to analyse the occurrence of yeasts and filamentous fungi in drinking water as well as to investigate their correlation with the indicator bacteria of faecal pollution. Yeasts were detected in 36.6% and 11.6% of the bottled mineral on water dispensers and tap water samples from municipal system, respectively. Twenty-one (35.0%) of bottled mineral water and two (3.3%) of tap water samples were positive for filamentous fungi. For bottled mineral water 12 (20.0%) of 60 samples were positive for total coliform, compared with 3(5.0%)out of 60 samples from tap water. The mineral water from dispensers was more contaminated than tap water. Strains belonging to the genera Candida identified to the species level were C. parapsilosis, C. glabrata and C. albicans. Thus, bottled mineral water from water dispensers and tap water could be considered a possible transmission route for filamentous fungi and yeasts, and could constitute a potential health hazard, mainly to immunocompromised indivuals.

BACTERICIDAL ACTIVITY OF THE BLOOD OF ACTIVELY IMMUNIZED WAX MOTH LARVAE

1962 ◽  
Vol 8 (4) ◽  
pp. 491-499 ◽  
Author(s):  
June M. Stephens
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibody Response ◽  
Bactericidal Activity ◽  
Whole Blood ◽  
Immune Serum ◽  
Shigella Dysenteriae ◽  
Immune State ◽  
The Third ◽  
Wax Moth

The blood of normal wax moth larvae is not bactericidal for Pseudomonas aeruginosa. The blood becomes moderately bactericidal when larvae are actively immunized against P. aeruginosa. This activity was measured by a modification of Nagington's technique for the estimation of typhoid antibody. Bactericidal activity appears to be the only measurable type of antibody response against P. aeruginosa. Cell-free blood was as active as whole blood; 0.02 ml of immune serum kills about 1000 organisms. The blood of normal wax moth larvae is bactericidal for Shigella dysenteriae but the blood of insects immunized against either this organism or P. aeruginosa shows no increase in activity against S. dysenteriae. A number of non-specific agents, both protein and non-protein, did not stimulate bactericidal activity in serum after their injection into normal larvae. Immune sera prepared against some strains of P. aeruginosa were not active against other strains. Storage at 37 °C or absorption with zymosan both result in blackening of immune blood and loss of bactericidal activity. Bactericidal activity is evident only during the immune state of the insect, i.e. from about 18 hours until the third day after vaccination; it develops at the same time that inhibition of melanization was observed in the blood from vaccinated larvae.

scholarly journals Presence of microcystins in the littoral zone of the Curonian Lagoon by the data of immunochromatographic analysis in 2017

2019 ◽  
Vol 4 (1) ◽  
pp. 109-111
Author(s):  
M. M. Smirnova
Keyword(s):  
Water Samples ◽  
Littoral Zone ◽  
Health Hazard ◽  
Curonian Lagoon ◽  
Curonian Spit ◽  
Potential Health ◽  
Potential Health Hazard ◽  
Inherent Problem ◽  
Strip Test ◽  
First Time

The presence of hepatotoxic cyanotoxins of microcystin group has been recorded in the southern part of the Curonian Lagoon annually since 2010. Because of unique natural features and cultural heritage, the Curonian Spit and the Curonian Lagoon are involved into diverse recreational usage. The potential health hazard due to microcystins is, thus, an inherent problem of the area. Rapid immunochromatographic semi-quantitative test (Microcystin Strip Test, Abraxis Ltd) was applied for the detection of microcystins in water samples from littoral zone. Samples were collected twice per month in May – November 2017 at six monitoring sites around the Lagoon. For the first time, the presence of toxic metabolites of cyanobacteria (microcystins) in the whole littoral of the southern part of the Curonian Lagoon during vegetation season was established. As previously noted, microcystins were present in water samples in summer and autumn. During May it were not found. The highest values (5–10 µg per litre) were recorded in the late June and during September at the Curonian Spit. These values were rather low in comparison with those of 2011–2016. From mid-to-late October toxins were recorded twice only, at the Curonian Spit. At the eastern and southern coasts maximal value was 5 µg per litre (once at the end of September). In other samples toxins were not detected or their values did not exceed 2.5 µg per litre.

scholarly journals BIOFILM FORMATION OF STAPHYLOCOCCUS AUREUS, SALMONELLA ENTERITIDIS AND PSEUDOMONAS AERUGINOSA IN PRESENCE OF N-BENZOTIAZOL-2-YL-BENZENSULFONAMIDE DERIVATIVES

2010 ◽  
Vol 0 (3(11)) ◽  
pp. 32-40
Author(s):  
I. О. Малярчик ◽  
Т. О. Філіпова ◽  
Т. О. Філіпова ◽  
Б. М. Галкін ◽  
Б. М. Галкін
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Salmonella Enteritidis

XÁC ĐỊNH THÀNH PHẦN HÓA HỌC VÀ KHẢ NĂNG KHÁNG VI SINH VẬT CỦA CAO CHIẾT ETHANOL LY TRÍCH TỪ THÂN RỄ LOÀI THIÊN NIÊN KIỆN PI-E (HOMALOMENA PIERREANA)

2020 ◽  
Vol 39 (03) ◽  
Author(s):  
VĂN HỒNG THIỆN ◽  
LÊ BÍCH TRÂM ◽  
NGUYỄN THANH LAN ◽  
HỒ NGUYỄN HOÀNG YẾN ◽  
LƯU HỒNG TRƯỜNG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis

Nghiên cứu này đã xác định được mẫu nghiên cứu thu tại Vườn quốc gia Phú Quốc là loài Homalomena pierreana. Thông qua kỹ thuật sắc ký ghép khối phổ, 10 hợp chất thuộc nhóm sesquiterpene có trong cao chiết ethanol của thân rễ loài H. pierreana đã được xác định. Ngoài ra, cao chiết ethanol từ mẫu nghiên cứu cũng cho thấy khả năng kháng lại 6 chủng vi khuẩn kiểm định là Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis và Salmonella typhimurium.

Detection of Coliform, faecal Coliform And Total Bacterial Count From Drinking Water Of Varanasi (U.P.) India

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Alok Kumar Singh ◽  
Anand Prakash Singh ◽  
Sanjay Srivastava
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Bacterial Count ◽  
Most Probable Number ◽  
Total Bacterial Count ◽  
Indicator Organisms ◽  
Cross Sectional ◽  
Probable Number ◽  
Hand Pump

In India source of drinking water at Varanasi city for common people are tap water, well, hand pump, Ganga river and stored tank water collected from bore well. All water samples were studied to assess their bacteriological characteristics and suitability for potable purposes. A cross-sectional epidemiological method was adopted to investigate the drinking water of six different sites of Varanasi city. The bacteriological examination of water samples included the most probable number of presumptive coliforms, faecal coliforms, and total bacterial count. The results showed that the total coliform count was detected in all the site. In all the methods coliforms presence was indicated. Maximum number of coliform observed in all the seasons, were from river and well water followed by hand pump, tap water and stored tank. The most common group of indicator organisms used in water quality monitoring are coliforms. These organisms are representative of bacteria normally present in the intestinal tract of mammals including human. Contamination of water may occur through different way like sewage disposal in the river, seepage of bathing near sites, fecal excreta of human, bird and other animals. Improving and expanding the existing water treatment and sanitation systems are more likely to provide good, safe and sustainable sources of water in the long term.

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