ANALYSIS OF CI SUBCOMPONENTS IN CELL CULTURE MEDIA OF HeLa CELLS AND DIPLOID HUMAN FETAL LUNG FIBROBLASTS

Gold nanorods (GNRs) are considered one of the most promising forms of nanoparticles for nanobiotechnology; however, the problem of their toxicity is currently not resolved. We synthesised GNRs, modified with linear polyethyleneimine (PEI-GNRs), and examined their physicochemical and some biological properties in comparison with GNRs modified with BSA and spherical gold nanoparticles (sGNPs) modified with the same agents. The influence of the buffer, cell culture media, and serum on hydrodynamic diameter and zeta potential of all GNPs was studied. Simultaneously, the size, shape, and formation of a corona were examined by transmission electron microscopy (TEM). PEI-GNRs and GNPs were nontoxic for BHK-21 and HeLa cells (MTT test). Penetration of all GNPs into BHK-21, melanoma B16, and HeLa cells was examined after 30 min, 3 h, and 24 h of incubation using TEM ultrathin sections. PEI-GNRs and PEI-sGNPs demonstrated fast and active penetration into cells by caveolin-dependent and lipid raft-mediated endocytosis and accumulated in endosomes and lysosomes. BSA-modified GNPs showed prolonged flotation and a significant delay in cell penetration. The results show that the charge of initial NPs determines penetration into cells. Thus, the designed PEI-GNRs were nontoxic and stable in cell culture media and could efficiently penetrate cells.

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Collagen secretion mediated by NMDR activation in human fetal lung fibroblasts

Cell Biology International ◽

10.1042/cbi034s070a ◽

2010 ◽

Vol 34 (8) ◽

pp. S70-S70

Author(s):

MingJie WANG ◽

ZiQiang LUO ◽

Mei LU ◽

LiHong SHANG ◽

ShaoJie YUE

Keyword(s):

Fetal Lung ◽

Lung Fibroblasts ◽

Collagen Secretion ◽

Human Fetal Lung

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Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽

10.1055/s-0036-1596271 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

KB Killday ◽

AS Freund ◽

C Fischer ◽

KL Colson

Keyword(s):

Cell Culture ◽

Culture Media ◽

Cell Culture Media ◽

Nutrient Consumption

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Targeted metabolomics in the cell culture media reveals increased uptake of branched amino acids by breast cancer cells

Analytical Biochemistry ◽

10.1016/j.ab.2021.114192 ◽

2021 ◽

pp. 114192

Author(s):

Fang Kou ◽

Bangjie Zhu ◽

Wenbin Zhou ◽

Chunming Lv ◽

Yu Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Amino Acids ◽

Cell Culture ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Culture Media ◽

Targeted Metabolomics ◽

Cell Culture Media ◽

Branched Amino Acids

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Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media

Microchemical Journal ◽

10.1016/j.microc.2021.106811 ◽

2021 ◽

pp. 106811

Author(s):

Yuanbin Guo ◽

Ming Shi ◽

Xiujuan Liu ◽

Huagang Liang ◽

Liming Gao ◽

...

Keyword(s):

Cell Culture ◽

Culture Media ◽

Dna Aptamer ◽

Cell Culture Media ◽

Preliminary Application

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MORPHOGENETIC ASPECTS OF MULTILAYERING IN PETRI DISH CULTURES OF HUMAN FETAL LUNG FIBROBLASTS

The Journal of Cell Biology ◽

10.1083/jcb.41.1.298 ◽

1969 ◽

Vol 41 (1) ◽

pp. 298-311 ◽

Cited By ~ 42

Author(s):

Tom Elsdale ◽

Robert Foley

Keyword(s):

Extracellular Matrix ◽

Human Lung ◽

Petri Dish ◽

Fetal Lung ◽

Single Species ◽

Lung Fibroblasts ◽

Human Lung Fibroblasts ◽

Spontaneous Aggregation ◽

Control Hypothesis ◽

Human Fetal Lung

Randomly seeded Petri dish cultures of embryonic human lung fibroblasts generate, in the course of their growth, highly ordered cellular arrangements. Thick, bilaterally symmetrical ridges with an axial polarity and an orthogonal, multilayered internal organization are observed within stationary cultures. The generation of these structures has been investigated. Ridges result from the spontaneous aggregation of cells in postconfluent cultures brought about by directed cell movements. These movements are promoted by the localized production of extracellular matrix sheets containing collagen, which provide new substrates for cellular colonization. Cells that have colonized one matrix substrate may secrete another above themselves, which will in turn be colonized. By a continuation of this cycle, thick stacks consisting of alternate layers of cells and matrix are produced to yield the observed aggregations. The distribution and shape of ridges in a culture imply that matrix substrates are confined to specific locations. The suggested control hypothesis assumes that all the cells in fibroblast cultures are potential producers of a single species of matrix. The serviceability of this matrix as a substrate for cellular colonization, however, is destroyed if the producer cells are motile. Matrix substrates, therefore, are only made by nonmotile cells.

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Benchmarking of commercially available CHO cell culture media for antibody production

Applied Microbiology and Biotechnology ◽

10.1007/s00253-015-6514-4 ◽

2015 ◽

Vol 99 (11) ◽

pp. 4645-4657 ◽

Cited By ~ 59

Author(s):

David Reinhart ◽

Lukas Damjanovic ◽

Christian Kaisermayer ◽

Renate Kunert

Keyword(s):

Cell Culture ◽

Antibody Production ◽

Culture Media ◽

Cho Cell ◽

Cell Culture Media ◽

Cho Cell Culture

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Intestinal and Hepatic Uptake of Dietary Peroxidized Lipids and Their Decomposition Products, and Their Subsequent Effects on Apolipoprotein A1 and Paraoxonase1

Antioxidants ◽

10.3390/antiox10081258 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1258

Author(s):

Xueting Jiang ◽

Pragney Deme ◽

Rajat Gupta ◽

Dmitry Litvinov ◽

Kathryn Burge ◽

...

Keyword(s):

Cell Culture ◽

Culture Media ◽

Degradation Products ◽

Apolipoprotein A1 ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Atherosclerotic Cardiovascular Disease ◽

Metabolic Fate ◽

Cell Culture Media ◽

Decomposition Products

Both pro- and antiatherosclerotic effects have been ascribed to dietary peroxidized lipids. Confusion on the role of peroxidized lipids in atherosclerotic cardiovascular disease is punctuated by a lack of understanding regarding the metabolic fate and potential physiological effects of dietary peroxidized lipids and their decomposition products. This study sought to determine the metabolic fate and physiological ramifications of 13-hydroperoxyoctadecadienoic acid (13-HPODE) and 13-HODE (13-hydroxyoctadecadienoic acid) supplementation in intestinal and hepatic cell lines, as well as any effects resulting from 13-HPODE or 13-HODE degradation products. In the presence of Caco-2 cells, 13-HPODE was rapidly reduced to 13-HODE. Upon entering the cell, 13-HODE appears to undergo decomposition, followed by esterification. Moreover, 13-HPODE undergoes autodecomposition to produce aldehydes such as 9-oxononanoic acid (9-ONA). Results indicate that 9-ONA was oxidized to azelaic acid (AzA) rapidly in cell culture media, but AzA was poorly absorbed by intestinal cells and remained detectable in cell culture media for up to 18 h. An increased apolipoprotein A1 (ApoA1) secretion was observed in Caco-2 cells in the presence of 13-HPODE, 9-ONA, and AzA, whereas such induction was not observed in HepG2 cells. However, 13-HPODE treatments suppressed paraoxonase 1 (PON1) activity, suggesting the induction of ApoA1 secretion by 13-HPODE may not represent functional high-density lipoprotein (HDL) capable of reducing oxidative stress. Alternatively, AzA induced both ApoA1 secretion and PON1 activity while suppressing ApoB secretion in differentiated Caco-2 cells but not in HepG2. These results suggest oxidation of 9-ONA to AzA might be an important phenomenon, resulting in the accumulation of potentially beneficial dietary peroxidized lipid-derived aldehydes.

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