Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

The herb of importance like Argyreia nervosa has shown wide range of pharmacological activities. Its methanolic extract of A. nervosa has been explored against Ehrlich ascites carcinoma (EAC) induced liquid and solid tumor in mice. Liquid and solid tumors were induced by intraperitoneal and subcutaneous transplantation of EAC cells in Balb/C mice. Significant and dose dependant results are observed when the mice are sacrificed on 15th day for estimation of tumor proliferation, hematological, biochemical and hepatic antioxidant parameters. Mean survival time (days) was increased to 36.5 from 20.5 extract treated mice. The extract also showed a decrease (p<0.001) in body weight and percentage reduction in tumor volume respectively when it was evaluated in solid tumor induced mice for a period of 30 days. From the result it was concluded that the extract has as a potent antitumor activity and that is comparable to 5-fluorouracil.

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THE ENZYMIC FORMATION OF 6-(METHYLMERCAPTO)PURINE RIBONUCLEOSIDE 5′-PHOSPHATE

Canadian Journal of Biochemistry ◽

10.1139/o66-027 ◽

1966 ◽

Vol 44 (2) ◽

pp. 229-245 ◽

Cited By ~ 44

Author(s):

Ian C. Caldwell ◽

J. Frank Henderson ◽

A. R. P. Paterson

Keyword(s):

Tumor Cells ◽

Blood Cells ◽

Intraperitoneal Injection ◽

Ehrlich Ascites Carcinoma ◽

Carcinoma Cells ◽

Ehrlich Ascites ◽

Mouse Tissues ◽

Enzymatic Methods

6-(Methylmercapto)purine ribonucleoside (Me6MPR) is efficiently phosphorylated in mouse tissues and in Ehrlich ascites carcinoma cells in vivo; tumor cells in vitro and cell-free extracts of the tumor also phosphorylate this analogue ribonucleoside. The product of this reaction has been identified by chemical and enzymatic methods and by its chromatographic behaviour as Me6MPR 5′-phosphate. The evidence presented in this report indicates that no other major metabolites of Me6MPR are formed.The phosphorylation of Me6MPR by cell-free tumor extracts requires ATP and Mn2+ (or Mg2+), and evidence is presented that the reaction is probably mediated by adenosine kinase.Me-14C-6MPR is rapidly taken up by most mouse tissues following its intraperitoneal injection. Forty minutes after injection of the labeled drug, the highest levels of radioactivity were found in intestine, liver, blood cells, lung, and spleen, in descending order; virtually no radioactivity was found in brain tissue or in blood plasma.

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Betulin-3,28-diphosphate as a Component of Combination Cytostatic Drugs for the Treatment of Ehrlich Ascites Carcinoma In Vitro and In Vivo Experiments

Scientia Pharmaceutica ◽

10.3390/scipharm86020017 ◽

2018 ◽

Vol 86 (2) ◽

pp. 17 ◽

Cited By ~ 2

Author(s):

Olga Vorobyova ◽

Olga Deryabina ◽

Darina Malygina ◽

Nadezhda Plotnikova ◽

Anna Solovyeva ◽

...

Keyword(s):

Ehrlich Ascites Carcinoma ◽

Cytostatic Drugs ◽

In Vivo Experiments ◽

Ehrlich Ascites

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The Anti-Tumoral Effects of Wolfberry (Lycium barbarum) on Experimentally Induced Ehrlich Ascites Carcinoma in Mice

Proceedings ◽

10.3390/proceedings2019040038 ◽

2019 ◽

Vol 40 (1) ◽

pp. 38

Author(s):

Uçar ◽

Ülger ◽

AL ◽

Nisari ◽

Karatoprak

Keyword(s):

Histopathological Examination ◽

Apoptotic Cell ◽

Ehrlich Ascites Carcinoma ◽

Lycium Barbarum ◽

In Vitro Techniques ◽

Cell Percentage ◽

Ehrlich Ascites ◽

Eat Cells

Wolfberry (Lycium barbarum) shows strengthen immune system, antioxidant and anticancerogenic effect. In this research, the effects of Wolfberry's extract fractions, cultivated in Kayseri, investigated on Ehrlich ascites tumor (EAT) using in vivo and in vitro techniques. For in vivo study, 200 mg/kg fractions of Wolfberry extract (above and below 50 kDa) were injected ip to EAT injected Balb/C mice. EAT cells were also cultured in the presence of Wolfberry's extract fractions (1500, 2000 µg/ml) to examine cell vitality and apoptosis using the Muse Cell Analyzer. Histopathological examination of intra-abdominal organs showed that there were decrease in the EAT cells adherence in the Wolfberry applied tissue groups when compared to the control. According to in vitro results, the both extract fractions (above and below 50 kDa) increased apoptosis in cancer cells. However, total apoptotic cell percentage was higher and statistically significant in groups above 50 kDa (1500, 2000 mg/ml) (p < 0.05). Previous literature and the results of the present study reveal that the consumption of wolfberry—which is also produced in Turkey—might be effective in preventing the formation of cancer and the deceleration of its progress.

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