Comparison of total carotenoids, lutein, zeaxanthin, and β‐carotene content in maize employing solvent extraction and in vitro physiological methods

2018 ◽  
Vol 42 (6) ◽  
pp. e12653
Author(s):  
Ashrafi Hossain ◽  
Padmanabhan Appukuttan Jayadeep
Keyword(s):  
Solvent Extraction ◽  
Total Carotenoids ◽  
Carotene Content ◽  
Β Carotene

Effect of phenol on Β-carotene content in total carotenoids production in cultivation ofRhodotorula glutinis

2004 ◽  
Vol 21 (3) ◽  
pp. 689-692 ◽  
Author(s):  
Bong Kyun Kim ◽  
Pyoung Kyu Park ◽  
Hee Jeong Chae ◽  
Eui Yong Kim
Keyword(s):  
Total Carotenoids ◽  
Carotene Content ◽  
Β Carotene

Influence of Pre- and Postharvest Factors on β-Carotene Content, Its in Vitro Bioaccessibility, and Antioxidant Capacity in Melons

2010 ◽  
Vol 58 (3) ◽  
pp. 1732-1740 ◽  
Author(s):  
Carla Maria Wolbang ◽  
Davinder Pal Singh ◽  
Stephen Richard Sykes ◽  
Jennifer Kay McInerney ◽  
Anthony Richard Bird ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Carotene Content ◽  
In Vitro Bioaccessibility ◽  
Β Carotene

Carotenoid Production by Lactoso-Negative Yeasts Co-Cultivated with Lactic Acid Bacteria in Whey Ultrafiltrate

2003 ◽  
Vol 58 (7-8) ◽  
pp. 562-567 ◽  
Author(s):  
Ginka I. Frengova ◽  
Simova D. Emilina ◽  
Dora M. Beshkova
Keyword(s):  
Lactobacillus Casei ◽  
Culture Medium ◽  
Carotenoid Content ◽  
Active Synthesis ◽  
Total Carotenoids ◽  
Carotene Content ◽  
Cell Accumulation ◽  
Initial Ph ◽  
Dry Cell ◽  
Β Carotene

Two strains were selected - the lactoso-negative yeast Rhodotorula rubra GED2 and the homofermentative Lactobacillus casei subsp. casei Ha1 for co-cultivation in cheese whey ultrafiltrate (WU) and active synthesis of carotenoids. Under conditions of intensive aeration (1.0 l/1 min, 220 rpm), a temperature of 30 °C, WU with 55.0 g lactose/l, initial pH = 5.5, the carotenoid content in the cells reached a maximum, when the growth of the cultures had come to an end, i.e. in the stationary phase of the yeast. The maxima for dry cell accumulation (27.0 g/l) and carotenoid formation (12.1 mg/l culture medium) did not coincide on the 5th and 6th day, respectively. A peculiarity of the carotenoid-synthesizing Rh. rubra GED2 strain, co-cultivated with L. casei Ha1, was the production of carotenoids with high β-carotene content (46.6% of total carotenoids) and 10.7% and 36.9% for torulene and torularhodin, respectively.

scholarly journals Insights of Sea Buckthorn Extract’s Encapsulation by Coacervation Technique

Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 59
Author(s):  
Diana Roman ◽  
Nina Nicoleta Condurache (Lazăr) ◽  
Iuliana Aprodu ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Total Carotenoids ◽  
Total Polyphenol ◽  
Color Structure ◽  
Bioactive Ingredients ◽  
The Stability ◽  
Β Carotene

Sea buckthorn (Hippophae rhamnoides L.) represents a valuable source of biologically active compounds such as carotenoids and polyphenols. High amounts of these substances are found in its fruits, bark, and leaves. However, their bioavailability is limited and must be increased in order to benefit from the properties they exert. Therefore, the purpose of this study was to increase the stability and bioavailability of sea buckthorn fruit’s bioactives. The sea buckthorn’s bioactive compounds were extracted with a solvent combination between glacial acetic acid, acetone, and water on one side and water only on the other side. Afterward, the phytochemicals from the extracts were encapsulated using the coacervation technique, followed by freeze-drying in order to obtain stable powders. The powders were characterized in terms of antioxidant activity, total carotenoids, β-carotene, lycopene, total polyphenol, and total flavonoid content, color, structure, and morphology. The phytochemical stability of the powders and their antioxidant activity was assessed during 270 days of storage at 4 °C. Moreover, the bioavailability of phytochemicals was measured during in vitro simulated digestibility. Our findings provide insights to promote carotenoids and polyphenols from sea buckthorn as bioactive ingredients with multiple purposes.

scholarly journals Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation

2020 ◽  
Vol 15 (1) ◽  
pp. 27-34
Author(s):  
Shilpa Pandurangaiah ◽  
Sadashiva A T ◽  
Shivashankar K S ◽  
SudhakarRao D V ◽  
Ravishankar K V
Keyword(s):  
High Performance ◽  
Color Space ◽  
Nondestructive Method ◽  
Carotenoid Content ◽  
Total Carotenoids ◽  
Time Saving ◽  
Carotene Content ◽  
Spectrophotometric Methods ◽  
Β Carotene ◽  
Cherry Tomatoes

Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin AA carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage.

scholarly journals Quantification of Chlorophyll and Carotene Pigments Content in Mountain Melick (Melica nutans L.) in Relation to Edaphic Variables

Forests ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1197
Author(s):  
Waldemar Zielewicz ◽  
Barbara Wróbel ◽  
Gniewko Niedbała
Keyword(s):  
Chlorophyll A ◽  
Middle Level ◽  
Chlorophyll B ◽  
Average Content ◽  
Site Location ◽  
Total Carotenoids ◽  
Carotene Content ◽  
Chlorophyll Pigments ◽  
Edaphic Variables ◽  
Β Carotene

The aim of the study was to identify and compare the content of chlorophyll and carotene pigments in mountain melick plants (Melica nutans L.) growing in two forest habitats in the Zielonka Forest Landscape Park in the Greater Poland region, which differed in soil properties and moisture. Leaf blades from the middle level of Melica nutans generative shoots were used as analytical material to measure the content of chlorophyll a, chlorophyll b, β-carotene and total carotenoids. The average (±SD) content of chlorophyll a in the Melica nutans plants growing in the less fertile site (Location I) amounted to 6.67 ± 0.99 mg·g−1 DM. The average content of chlorophyll b in the same plants was 2.44 ± 0.39 mg·g−1 DM. The Melica nutans plants growing in the more fertile site (Location II) had a higher content of chlorophyll a, i.e., 7.76 ± 0.96 mg·g−1 DM, and lower content of chlorophyll b, i.e., 2.36 ± 0.26 mg·g−1 DM. The plants collected from both sites had similar content of β-carotene, i.e., 0.61 ± 0.11 mg·g−1 DM Location I and 0.62 ± 0.07 mg·g−1 DM Location II. The plants growing in the less fertile site (Location I) had significantly higher total carotene content than the plants in the more fertile site (Location II). The content of pigments in the Melica nutans plants was significantly differentiated by the meteorological conditions in the subsequent years of the research. In the first year of the research, the average content of all chlorophyll pigments in the plants was significantly higher than in the second drier year, regardless of the site.

scholarly journals β-Carotene Micellarization during in Vitro Digestion and Uptake by Caco-2 Cells Is Directly Proportional to β-Carotene Content in Different Genotypes of Cassava

2007 ◽  
Vol 137 (10) ◽  
pp. 2229-2233 ◽  
Author(s):  
Sagar K. Thakkar ◽  
Bussie Maziya-Dixon ◽  
Alfred G. O. Dixon ◽  
Mark L. Failla
Keyword(s):  
In Vitro Digestion ◽  
Carotene Content ◽  
Β Carotene

scholarly journals EFFECT OF COOKING ON THE CONTENT OF CAROTENOIDS AND TOCOPHEROLS IN SWEET CORN

2021 ◽  
Author(s):  
Snežana Mladenović Drinić ◽  
Jelena Vukadinović ◽  
Jelena Srdić ◽  
Marija Milašinović Šeremešić ◽  
Violeta Andjelkovic
Keyword(s):  
High Performance ◽  
Nutritional Value ◽  
Sweet Corn ◽  
Tocopherol Content ◽  
Mean Value ◽  
Total Carotenoids ◽  
Corn Hybrids ◽  
Carotene Content ◽  
Before And After ◽  
Β Carotene

Taste and nutritional value make sweet corn a valued plant and an important component of the human diet worldwide. Kernel nutritive composition of sweet corn has been reported in various papers, but a description of carotenoid and tocopherols profile, especially after cooking is scarce. Therefore, the present study was carried out to compare the carotenoid and tocopherol content in sweet corn before and after cooking. Contents of β-carotene, lutein+zeaxanthin and tocopherols (δ-T, β+γ-T, α-T) in the kernels of twelve sweet corn hybrids were determined by High-Performance Liquid Chromatography (HPLC) and were expressed as the mean value of three independent measurements. Both genotype and cooking affected the content of the carotenoids and tocopherols in the kernel. The highest content of total carotenoids before and after cooking was found in hybrid ZP486/1su (27.77/45.28 µg/g) whereas the lowest content was in hybrid ZP 355su (10.27 µg/g) before cooking i.e. in hybrid ZP 347su (24.55 µg/g) after cooking. The cooking resulted in a significant increase in the content of total carotenoids and tocopherols, lutein+zeaxanthin, and β-carotene in all hybrids, except the ZP504su in which the β-carotene content decreased. An increase in α-tocopherol after cooking was observed in hybrids ZP485/1su and ZP484/1su, while a decrease was in hybrids ZP481/1su, ZP486/1su and ZP477/2su. The results showed that increasing micronutrient content is genotype-dependent. This study confirmed that cooking increases the nutritional value of sweet corn and gives it additional value in terms of functional food.

scholarly journals Analysis of Lipophilic Antioxidants in the Leaves of Kaempferia parviflora Wall. Ex Baker Using LC–MRM–MS and GC–FID/MS

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1573
Author(s):  
Kihwan Song ◽  
Ramesh Kumar Saini ◽  
Young-Soo Keum ◽  
Iyyakkannu Sivanesan
Keyword(s):  
Bioactive Metabolites ◽  
Vitamin K1 ◽  
Ex Vitro ◽  
Kaempferia Parviflora ◽  
Total Carotenoids ◽  
Lipophilic Compounds ◽  
Potential Source ◽  
Lipophilic Antioxidant ◽  
Β Carotene

Lipophilic metabolites such as carotenoids, fatty acids, vitamin K1, phytosterols, and tocopherols are important antioxidants that are used in the cosmetics, foods, and nutraceutical industries. Recently, there has been a growing demand for the use of byproducts (wastes) as a potential source of industrially important compounds. The leaves of Kaempferia parviflora (black ginger) (KP-BG) are major byproducts of KP-BG cultivation and have been reported to contain several bioactive metabolites; however, the composition of lipophilic metabolites in KP-BG leaves has not been examined. In this study, the lipophilic antioxidant profile was analyzed in the leaves of KP-BG plants grown in vitro and ex vitro. Lipophilic compounds, namely carotenoids (80.40–93.84 µg/g fresh weight (FW)), tocopherols (42.23–46.22 µg/g FW), phytosterols (37.69–44.40 µg/g FW), and vitamin K1 (7.25–7.31 µg/g FW), were quantified using LC–MRM–MS. The fatty acid profile of the KP-BG leaves was identified using GC–FID/MS. The content of individual lipophilic compounds varied among the KP-BG leaves. Ex vitro KP-BG leaves had high levels of lutein (44.38 µg/g FW), α-carotene (14.79 µg/g FW), neoxanthin (12.30 µg/g FW), β-carotene (11.33 µg/g FW), violaxanthin (11.03 µg/g FW), α-tocopherol (39.70 µg/g FW), α-linolenic acid (43.12%), palmitic acid (23.78%), oleic acid (12.28%), palmitoleic acid (3.64%), total carotenoids (93.84 µg/g FW), and tocopherols (46.22 µg/g FW) compared with in vitro KP-BG leaves. These results indicate that ex-vitro-grown KP-BG leaves could be used as a valuable natural source for extracting important lipophilic antioxidants.

scholarly journals Dietary supplementation with carotenoids: effects on α-tocopherol levels and susceptibility of tissues to oxidative stress

1996 ◽  
Vol 76 (2) ◽  
pp. 307-317 ◽  
Author(s):  
Alan A. Woodall ◽  
George Britton ◽  
Malcolm J. Jackson
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Control Diet ◽  
Oxidant Stress ◽  
Cumene Hydroperoxide ◽  
Dietary Supplementation ◽  
Significant Activity ◽  
Total Carotenoids ◽  
Β Carotene

The ability of dietary supplementation with carotenoids to protect chick tissues against oxidative stress in vitro was examined. Male Leghorn chicks were fed on diets supplemented (100 mg supplement/kg diet) with either β-carotene, zeaxanthin (β,β-carotene-3,3'-diol), canthaxanthin (β,β-carotene-4,4'-dione) or α-tocopherol, or on a control diet, from 1 d old until 37 d of age. Tissues (liver, heart, skeletal muscle and plasma) were removed and assayed for total carotenoids and α-tocopherol content and portions subjected to oxidative stress by incubation of homogenates with cumene hydroperoxide and FeSO4. Animals receiving zeaxanthin and canthaxanthin had significantly greater carotenoid concentrations in liver, heart, muscle and plasma compared with untreated controls (P < 0·05); animals fed on diets supplemented with β-carotene or α-tocopherol did not have significantly different tissue carotenoid contents compared with untreated controls. α-Tocopherol supplementation elevated α-tocopherol levels in all tissues examined (P < 0·05). Supplementation with caroteuoids did not affect tissue α-tocopherol levels, but β-carotene lowered plasma α-tocopherol levels by 50% (P < 0·05). Incubation of plasma or tissue homogenates with oxidant stressors induced lipid peroxidation (production of thiobarbituric-acid reactive substances) in all tissues. Animals given α-tocopherol, β-carotene or zeaxanthin had a reduced susceptibility to oxidant stress in liver compared with unsupplemented controls (P < 0·05), and α-tocopherol-supplemented animals had reduced susceptibility in skeletal muscle compared with unsupplemented controls (P < 0·05). Canthaxanthin supplementation did not influence the susceptibility to oxidant stress in any tissue examined. These results suggest that zeaxanthin, a carotenoid present in animal and human diets, may have significant activity as an antioxidant against oxidative stress in tissues.

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