scholarly journals Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation

2020 ◽  
Vol 15 (1) ◽  
pp. 27-34
Author(s):  
Shilpa Pandurangaiah ◽  
Sadashiva A T ◽  
Shivashankar K S ◽  
SudhakarRao D V ◽  
Ravishankar K V
Keyword(s):  
High Performance ◽  
Color Space ◽  
Nondestructive Method ◽  
Carotenoid Content ◽  
Total Carotenoids ◽  
Time Saving ◽  
Carotene Content ◽  
Spectrophotometric Methods ◽  
Β Carotene ◽  
Cherry Tomatoes

Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin AA carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage.

scholarly journals Correlation of High Performance Liquid Chromatography (HPLC) and Spectrophotometric Methods to Assess the Post Harvest Storage and Processing Changes in Total β-carotene Contents in Selected Nigeria Vegetables

2020 ◽  
pp. 22-39
Author(s):  
Emeka Felix Okpalanma
Keyword(s):  
High Performance ◽  
Storage Conditions ◽  
Leafy Vegetables ◽  
Coefficient Of Determination ◽  
Carotene Content ◽  
Spectrophotometric Methods ◽  
Domestic Processing ◽  
Content Range ◽  
Β Carotene ◽  
Temperature Storage

The aim of this study was to correlate analytical methods (HPLC and spectrophotometric) in assessing the changes in total β-carotene contents in leafy vegetables during ambient temperature storage (29+2°C) and domestic processing (5 min,100°C.) The vegetables analyzed were: Telfairia occidentalis, Amaranethus hybridus, Talinum triangulare,Pterocarpus mildbraedli and Gnetum africanum. Total–carotene was determined spectrophometrically, while HPLC was used for detailed analysis of carotenoides. Lutein, β -cryptoxanthin and β-carotene isomers were identified and quantified. Results indicated that the raw vegetables were rich in lutein (124.03-655.95 µg/gdwt) and total β -carotene (45.42 – 246.93 µg/gdwt). Beta–cryptoxanthin was detected in small quantity (5.05-11 µg/gdwt). However, spectrophotometric result indicated a total–carotene content range (186.10 – 953.78 µg/gdwt). Cooking increased significantly (P< 0.05), the lutein (382. 92 – 1158.83 µg/gdwt), total β -carotene (738.53 – 1756.51 µg/gdwt) contents of the samples, however, it decreased the % trans–β-carotene contents. Storage conditions in the study increased significantly (P< 0.05) the contents of total β -carotene and total–carotene except in the case of Gnetum africanum leaf. A regression model for the two methods of analysis of β -carotene with a coefficient of correlation r = 0.925 and coefficient of determination r2 = 0.856, which allows for the calculation of total β - carotene from total-carotene content was obtained.

Carotenoid Production by Lactoso-Negative Yeasts Co-Cultivated with Lactic Acid Bacteria in Whey Ultrafiltrate

2003 ◽  
Vol 58 (7-8) ◽  
pp. 562-567 ◽  
Author(s):  
Ginka I. Frengova ◽  
Simova D. Emilina ◽  
Dora M. Beshkova
Keyword(s):  
Lactobacillus Casei ◽  
Culture Medium ◽  
Carotenoid Content ◽  
Active Synthesis ◽  
Total Carotenoids ◽  
Carotene Content ◽  
Cell Accumulation ◽  
Initial Ph ◽  
Dry Cell ◽  
Β Carotene

Two strains were selected - the lactoso-negative yeast Rhodotorula rubra GED2 and the homofermentative Lactobacillus casei subsp. casei Ha1 for co-cultivation in cheese whey ultrafiltrate (WU) and active synthesis of carotenoids. Under conditions of intensive aeration (1.0 l/1 min, 220 rpm), a temperature of 30 °C, WU with 55.0 g lactose/l, initial pH = 5.5, the carotenoid content in the cells reached a maximum, when the growth of the cultures had come to an end, i.e. in the stationary phase of the yeast. The maxima for dry cell accumulation (27.0 g/l) and carotenoid formation (12.1 mg/l culture medium) did not coincide on the 5th and 6th day, respectively. A peculiarity of the carotenoid-synthesizing Rh. rubra GED2 strain, co-cultivated with L. casei Ha1, was the production of carotenoids with high β-carotene content (46.6% of total carotenoids) and 10.7% and 36.9% for torulene and torularhodin, respectively.

scholarly journals EFFECT OF COOKING ON THE CONTENT OF CAROTENOIDS AND TOCOPHEROLS IN SWEET CORN

2021 ◽  
Author(s):  
Snežana Mladenović Drinić ◽  
Jelena Vukadinović ◽  
Jelena Srdić ◽  
Marija Milašinović Šeremešić ◽  
Violeta Andjelkovic
Keyword(s):  
High Performance ◽  
Nutritional Value ◽  
Sweet Corn ◽  
Tocopherol Content ◽  
Mean Value ◽  
Total Carotenoids ◽  
Corn Hybrids ◽  
Carotene Content ◽  
Before And After ◽  
Β Carotene

Taste and nutritional value make sweet corn a valued plant and an important component of the human diet worldwide. Kernel nutritive composition of sweet corn has been reported in various papers, but a description of carotenoid and tocopherols profile, especially after cooking is scarce. Therefore, the present study was carried out to compare the carotenoid and tocopherol content in sweet corn before and after cooking. Contents of β-carotene, lutein+zeaxanthin and tocopherols (δ-T, β+γ-T, α-T) in the kernels of twelve sweet corn hybrids were determined by High-Performance Liquid Chromatography (HPLC) and were expressed as the mean value of three independent measurements. Both genotype and cooking affected the content of the carotenoids and tocopherols in the kernel. The highest content of total carotenoids before and after cooking was found in hybrid ZP486/1su (27.77/45.28 µg/g) whereas the lowest content was in hybrid ZP 355su (10.27 µg/g) before cooking i.e. in hybrid ZP 347su (24.55 µg/g) after cooking. The cooking resulted in a significant increase in the content of total carotenoids and tocopherols, lutein+zeaxanthin, and β-carotene in all hybrids, except the ZP504su in which the β-carotene content decreased. An increase in α-tocopherol after cooking was observed in hybrids ZP485/1su and ZP484/1su, while a decrease was in hybrids ZP481/1su, ZP486/1su and ZP477/2su. The results showed that increasing micronutrient content is genotype-dependent. This study confirmed that cooking increases the nutritional value of sweet corn and gives it additional value in terms of functional food.

scholarly journals Correlation Between L*a*b* Color Space Values and Carotenoid Content in Pumpkins and Squash (Cucurbita spp.)

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 633-637 ◽  
Author(s):  
Rachel A. Itle ◽  
Eileen A. Kabelka
Keyword(s):  
High Performance ◽  
Block Design ◽  
Color Space ◽  
Carotenoid Content ◽  
Regression Equations ◽  
Strong Correlations ◽  
Total Carotenoids ◽  
Highly Sensitive ◽  
Reproducible Method ◽  
Color Value

Carotenoids play an important role in human health by acting as sources of provitamin A or as protective antioxidants. Pumpkins and squash (Cucurbita spp.) are excellent dietary sources of carotenoids. The diversity and range of carotenoid types and concentrations within pumpkins and squash provide a means to increase the nutritional value of this crop through plant breeding. Breeding requires reliable estimates of carotenoid types and concentrations to distinguish differences among breeding material. One method used for carotenoid identification and quantification is high-performance liquid chromatography (HPLC). It is a highly sensitive and reproducible method but expensive and time-consuming. In contrast, colorimeters objectively describing visible color are relatively inexpensive and easy to use. The objective of this research was to determine if the carotenoid content within pumpkin and squash measured by HPLC was correlated with colorimeter L*a*b* color space values. Cultigens (cultivars, heirlooms, and PIs) representing white, yellow, and orange flesh color were grown at multiple locations using a randomized complete block design with two replicates at each location. Fruit flesh of each cultigen was evaluated using HPLC and colorimetric analysis. Strong correlations were found between color value a* and total carotenoids (r = 0.91) and color value b* and chroma with lutein (r = 0.87). Regression equations based on these correlations will be useful for estimating carotenoid type and concentrations. These close associations will also assure that breeding for enhanced carotenoid content within pumpkins and squash can be achieved using an easy-to-use and inexpensive method.

scholarly journals Variation in β-Carotene and Total Carotenoid Content in Fruits of Capsicum

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 746-749 ◽  
Author(s):  
Marisa M. Wall ◽  
Cynthia A. Waddell ◽  
Paul W. Bosland
Keyword(s):  
High Performance ◽  
Dry Weight ◽  
Carotenoid Content ◽  
Total Carotenoid ◽  
Fresh Weight ◽  
Ripe Fruit ◽  
Total Carotenoids ◽  
New Mexican ◽  
Total Carotenoid Content ◽  
Β Carotene

The β-carotene and total carotenoid content of either fresh or dried tissue of fruits of a total of 57 cultivars of six Capsicum species were analyzed using high performance liquid chromatography (HPLC). β-Carotene levels in ripe fruit varied from 0 to 166 μg·g-1 fresh weight, and carotenoid levels were from 1 to 896 μg·g-1 in ripe fruit in 1996. The range of values for β-carotene was similar in 1997, but that for total carotenoids was wider (4 to 1173 μg·g-1 fresh weight). Fresh fruit of the cultivars Greenleaf Tabasco, Pulla, Guajillo, NuMex Conquistador, Ring-O-Fire, and Thai Dragon contained greater amounts of β-carotene per 100 g fresh weight than the recommended dietary allowance (RDA) for vitamin A for the average adult. For dried Capsicum entries, New Mexican, aji, pasilla, and ancho types had the highest levels of β-carotene. In 1996, β-carotene levels among the dried Capsicum germplasm ranged from 2 to 739 μg·g-1 dry weight, and carotenoid levels from 111 to 6226 μg·g-1. Values were higher in 1997, ranging from 24 to 1198 μg·g-1 dry weight for β-carotene and from 187 to 10,121 μg·g-1 for total carotenoids. A pasilla type (C. annuum L.) had the highest total carotenoid content among the dried entries in both years.

scholarly journals Quality characterization, phenolic and carotenoid content of new orange, cream and yellow-fleshed sweetpotato genotypes

2021 ◽  
Vol 39 (3) ◽  
pp. 299-304
Author(s):  
Lucimeire Pilon ◽  
Jaqueline S Guedes ◽  
Bruna S Bitencourt ◽  
Raphael Augusto de C Melo ◽  
Larissa PC Vendrame ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ipomoea Batatas ◽  
Dry Matter ◽  
Matter Content ◽  
Soluble Solids ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Dry Matter Content ◽  
Total Carotenoids ◽  
Β Carotene

ABSTRACT Sweetpotato (Ipomoea batatas) is a root crop grown in many countries. This tuberous root is a source of energy, nutrients, and phytochemicals. In this study, bioactive compounds and physical and physicochemical qualities of sweetpotato genotypes were evaluated. Eight new genotypes of sweetpotato produced by Embrapa Hortaliças (orange-fleshed: MD09026-OF and MD09024-OF; cream-fleshed: MD09011-CF, MD09004-CF, MD10039-CF, and MD10004-CF; yellow-fleshed: MD09017-YF and MD12002-YF) and two cultivars used as controls (Beauregard and Brazlândia Roxa) were evaluated for color, soluble solids, dry matter, phenolic compounds, total carotenoids and β-carotene. Hue angles differed even between those sweetpotatoes with the same flesh color. The orange-fleshed genotypes MD09024-OF, MD09026-OF, and Beauregard, had the lowest L*, showing to be darker than the others. These sweetpotatoes also had the brightest flesh colors with higher C*. The orange-fleshed genotypes MD09026-OF and MD09024-OF were sweeter (10.55oBrix and 9.23oBrix) than Beauregard (5.12oBrix). Brazlândia Roxa had the highest dry matter content (38.05%), followed by the genotypes MD10004-CF, MD09017-YF, MD09026-OF MD10039-CF, and MD09011-CF, which showed similarity, ranging from 32.33% to 29.12%. The highest contents of total carotenoids were found for the orange-fleshed genotypes MD09026-OF (80.06 mg g-1) and MD09024-OF (70.56 mg g-1) and Beauregard (73.12 mg g-1). These same genotypes showed the highest total phenolic compounds (0.815 mg g-1 and 0.686 mg g-1, respectively). MD09026-OF showed the highest content of β-carotene (46.47 mg g-1). MD09026-OF was the most prominent genotype among those evaluated, as it showed the highest total carotenoid, β-carotene, phenolic compounds, and soluble solids content, in addition to a high dry matter content.

scholarly journals Stability of Carotenoids in Dried Apricots (Prunus Armeniaca L.) During Storage

2016 ◽  
Vol 73 (2) ◽  
pp. 93 ◽  
Author(s):  
Elena Andreea POP ◽  
Andrea BUNEA ◽  
Florina COPACIU ◽  
Carmen SOCACIU ◽  
Adela PINTEA
Keyword(s):  
Biological Effects ◽  
Carotenoid Content ◽  
Limited Information ◽  
Photodiode Array Detection ◽  
Total Carotenoids ◽  
Residual Concentration ◽  
Geometrical Isomers ◽  
Total Carotenoid Content ◽  
The Stability ◽  
Β Carotene

Apricots are well known for the high content of bioactive compounds such as carotenoids, polyphenols, vitamins and minerals. Several studies have pointed out the chemical composition or the biological effects of apricots, but limited information are available regarding the stability of active compounds during storage or processing. The aim of this study was to determine the stability of major carotenoids in commercial dried apricots during storage.Carotenoids were extracted monthly from dried apricots kept in a dark environment, at room temperature, for twelve months. Total carotenoids were determined using the spectrophotometric method while the most relevant carotenoids were analyzed by high-performance liquid chromatography-photodiode array detection (HPLC-PDA) on a C30 column and using a gradient elution system.Initial carotenoid content of dried fruits was 6.72 mg/100g, while after six months of storage it decreased to 2.46 mg/100g. After twelve months of storage the total carotenoid content was 0.82 mg/100g, representing 20.35 % of the initial concentration. The major carotenoids identified in apricots were: all trans β-carotene, its geometrical isomers (9-cis-β-carotene; 13-cis-β-carotene; 9,13-di-cis β-carotene); β-carotene-5,8-epoxide; β-cryptoxanthin and β-cryptoxanthin palmitate. Significant decreases were observed for all pigments but all trans β-carotene appears to be the most sensitive pigment, with 15.7 % residual concentration. Although the concentrations of β-cryptoxanthin palmitate is small, it has shown significant increased stability compared to carotenes.

scholarly journals Effect of Thermal Processing on Carotenoids and Folate Changes in Six Varieties of Sweet Potato (Ipomoes batata L.)

Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 215 ◽  
Author(s):  
Zhijun Pan ◽  
Yiming Sun ◽  
Fangyuan Zhang ◽  
Xinbo Guo ◽  
Zhihua Liao
Keyword(s):  
Sweet Potato ◽  
Thermal Processing ◽  
Thermal Process ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Significant Loss ◽  
Thermal Impact ◽  
Total Carotenoids ◽  
Microbial Assay ◽  
Β Carotene

Carotenoids and folate are two mandatory supplying micronutrients for children or pregnant women. Inadequate intake of these two nutrients was relevant to a higher mortality of both children and pregnancies. This study is intended to investigate the thermal impact on the changes of carotenoids and folate in sweet potato roots (SPRs). Carotenoids were identified by high performance liquid chromatography (HPLC) while the folate was estimated using a microbial assay. An obvious decline was observed in total carotenoids after heating. Nevertheless, the content of provitamin compound β-carotene exhibited incredible stability during steaming and α-carotene multiplied in certain varieties, evidencing that SPRs could be an efficient way for addressing Vitamin A deficiency (VAD). As for the total folate contents, two varieties were found no significant loss after thermal process while the others showed a significant decrease. The results indicated that steaming process led to generally loss of both carotenoids and folate while the α-carotene and β-carotene were well preserved. The information provided by this study might help with enhancing the food quality in processing industry and the understanding in the nutrition changes during steaming.

scholarly journals Evaluation of Metabolites and Antioxidant Activity in Pumpkin Species

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2092098 ◽  
Author(s):  
Hafiz Muhammad Khalid Abbas ◽  
He-Xun Huang ◽  
Wen-Jie Huang ◽  
Shu-Dan Xue ◽  
Shi-Juan Yan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Cucurbita Moschata ◽  
Cucurbita Maxima ◽  
Total Carotenoids ◽  
Β Carotene

Cucurbita species ( Cucurbita moschata and Cucurbita maxima) are rich sources of nutrients, especially carotenoids and carbohydrates. Metabolites in pulps of C. moschata and C. maxima lines were examined by high-performance liquid chromatography and gas chromatography–mass spectrometry. Our results revealed that glucose and sucrose were the dominant sugars in C. maxima and C. moschata, respectively. A correlation was found between Brix percentage and sucrose levels as compared with glucose and fructose. A greater amount of myo-inositol had accumulated in C. moschata lines compared with C. maxima. Conversely, total carotenoids and antioxidant activity were found to be higher in C. maxima lines than in C. moschata. A strong correlation of glucose, fructose, and sucrose with β-carotene and violaxanthin revealed that it will be difficult to develop pumpkin cultivars with both high inositol and carotenoid levels. In conclusion, the composition of carbohydrates and carotenoids was more diverse in C. moschata lines than in C. maxima lines. Our results will contribute to a better understanding of metabolite changes in the fruits of these as well as other pumpkin species.

Effect of phenol on Β-carotene content in total carotenoids production in cultivation ofRhodotorula glutinis

2004 ◽  
Vol 21 (3) ◽  
pp. 689-692 ◽  
Author(s):  
Bong Kyun Kim ◽  
Pyoung Kyu Park ◽  
Hee Jeong Chae ◽  
Eui Yong Kim
Keyword(s):  
Total Carotenoids ◽  
Carotene Content ◽  
Β Carotene
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