A cold responsive galactinol synthase gene fromMedicago falcata(MfGolS1) is induced bymyo-inositol and confers multiple tolerances to abiotic stresses

2013 ◽  
Vol 149 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Chunliu Zhuo ◽  
Ting Wang ◽  
Shaoyun Lu ◽  
Yaqing Zhao ◽  
Xiaoguang Li ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Galactinol Synthase ◽  
Multiple Tolerances

scholarly journals Responses of Populus trichocarpa galactinol synthase genes to abiotic stresses

2013 ◽  
Vol 127 (2) ◽  
pp. 347-358 ◽  
Author(s):  
Jie Zhou ◽  
Yang Yang ◽  
Juan Yu ◽  
Like Wang ◽  
Xiang Yu ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Populus Trichocarpa ◽  
Galactinol Synthase

scholarly journals Genome-Wide Identification and Expression Profiling Analysis of the Galactinol Synthase Gene Family in Cassava (Manihot esculenta Crantz)

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 250 ◽  
Author(s):  
Ruimei Li ◽  
Shuai Yuan ◽  
Yingdui He ◽  
Jie Fan ◽  
Yangjiao Zhou ◽  
...  
Keyword(s):  
Gene Family ◽  
Abiotic Stresses ◽  
Manihot Esculenta ◽  
Expression Patterns ◽  
Pcr Analysis ◽  
Systematic Research ◽  
Raffinose Family Oligosaccharides ◽  
Manihot Esculenta Crantz ◽  
Galactinol Synthase ◽  
Genome Wide

Galactinol synthases (GolSs) are the key enzymes that participate in raffinose family oligosaccharides (RFO) biosynthesis, which perform a big role in modulating plant growth and response to biotic or abiotic stresses. To date, no systematic study of this gene family has been conducted in cassava (Manihot esculenta Crantz). Here, eight MeGolS genes are isolated from the cassava genome. Based on phylogenetic background, the MeGolSs are clustered into four groups. Through predicting the cis-elements in their promoters, it was discovered that all MeGolS members act as hormone-, stress-, and tissue-specific related elements to different degrees. MeGolS genes exhibit incongruous expression patterns in various tissues, indicating that different MeGolS proteins might have diverse functions. MeGolS1 and MeGolS3–6 are highly expressed in leaves and midveins. MeGolS3–6 are highly expressed in fibrous roots. Quantitative real-time Polymerase Chain Reaction (qRT-PCR) analysis indicates that several MeGolSs, including MeGolS1, 2, 5, 6, and 7, are induced by abiotic stresses. microRNA prediction analysis indicates that several abiotic stress-related miRNAs target the MeGolS genes, such as mes-miR156, 159, and 169, which also respond to abiotic stresses. The current study is the first systematic research of GolS genes in cassava, and the results of this study provide a basis for further exploration the functional mechanism of GolS genes in cassava.

scholarly journals Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Christina C. Vinson ◽  
Ana P. Z. Mota ◽  
Brenda N. Porto ◽  
Thais N. Oliveira ◽  
Iracyara Sampaio ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Expression Profiling ◽  
Abiotic Stresses ◽  
Abiotic Stress Tolerance ◽  
Distribution Patterns ◽  
Grain Legumes ◽  
Raffinose Family Oligosaccharides ◽  
Galactinol Synthase ◽  
Raffinose Synthase ◽  
Gene Structures

Abstract Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties in grain legumes, little information is available about the enzymes involved in RFO metabolism in Fabaceae species. In the present study, the systematic survey of legume proteins belonging to five key enzymes involved in the metabolism of RFOs (galactinol synthase, raffinose synthase, stachyose synthase, alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes in Arachis duranensis and 31 in A. ipaënsis. Their phylogenetic relationships, gene structures, protein domains, and chromosome distribution patterns were also determined. Based on the expression profiling of these genes under water deficit treatments, a galactinol synthase candidate gene (AdGolS3) was identified in A. duranensis. Transgenic Arabidopsis plants overexpressing AdGolS3 exhibited increased levels of raffinose and reduced stress symptoms under drought, osmotic, and salt stresses. Metabolite and expression profiling suggested that AdGolS3 overexpression was associated with fewer metabolic perturbations under drought stress, together with better protection against oxidative damage. Overall, this study enabled the identification of a promising GolS candidate gene for metabolic engineering of sugars to improve abiotic stress tolerance in crops, whilst also contributing to the understanding of RFO metabolism in legume species.

scholarly journals A Sugarcane G-Protein-Coupled Receptor, ShGPCR1, Confers Tolerance to Multiple Abiotic Stresses

2021 ◽  
Vol 12 ◽  
Author(s):  
Manikandan Ramasamy ◽  
Mona B. Damaj ◽  
Carol Vargas-Bautista ◽  
Victoria Mora ◽  
Jiaxing Liu ◽  
...  
Keyword(s):  
G Protein ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Late Embryogenesis Abundant ◽  
Marker Genes ◽  
Galactinol Synthase ◽  
Sugarcane Yield ◽  
Transgenic Lines ◽  
Constitutive Overexpression ◽  
G Protein Coupled

Sugarcane (Saccharum spp.) is a prominent source of sugar and serves as bioenergy/biomass feedstock globally. Multiple biotic and abiotic stresses, including drought, salinity, and cold, adversely affect sugarcane yield. G-protein-coupled receptors (GPCRs) are components of G-protein-mediated signaling affecting plant growth, development, and stress responses. Here, we identified a GPCR-like protein (ShGPCR1) from sugarcane and energy cane (Saccharum spp. hybrids) and characterized its function in conferring tolerance to multiple abiotic stresses. ShGPCR1 protein sequence contained nine predicted transmembrane (TM) domains connected by four extracellular and four intracellular loops, which could interact with various ligands and heterotrimeric G proteins in the cells. ShGPCR1 sequence displayed other signature features of a GPCR, such as a putative guanidine triphosphate (GTP)-binding domain, as well as multiple myristoylation and protein phosphorylation sites, presumably important for its biochemical function. Expression of ShGPCR1 was upregulated by drought, salinity, and cold stresses. Subcellular imaging and calcium (Ca2+) measurements revealed that ShGPCR1 predominantly localized to the plasma membrane and enhanced intracellular Ca2+ levels in response to GTP, respectively. Furthermore, constitutive overexpression of ShGPCR1 in sugarcane conferred tolerance to the three stressors. The stress-tolerance phenotype of the transgenic lines corresponded with activation of multiple drought-, salinity-, and cold-stress marker genes, such as Saccharum spp. LATE EMBRYOGENESIS ABUNDANT, DEHYDRIN, DROUGHT RESPONSIVE 4, GALACTINOL SYNTHASE, ETHYLENE RESPONSIVE FACTOR 3, SALT OVERLY SENSITIVE 1, VACUOLAR Na+/H+ ANTIPORTER 1, NAM/ATAF1/2/CUC2, COLD RESPONSIVE FACTOR 2, and ALCOHOL DEHYDROGENASE 3. We suggest that ShGPCR1 plays a key role in conferring tolerance to multiple abiotic stresses, and the engineered lines may be useful to enhance sugarcane production in marginal environments with fewer resources.

Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses

2011 ◽  
Vol 49 (4) ◽  
pp. 441-448 ◽  
Author(s):  
Tiago B. dos Santos ◽  
Ilara G.F. Budzinski ◽  
Celso J. Marur ◽  
Carmen L.O. Petkowicz ◽  
Luiz F.P. Pereira ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Coffea Arabica ◽  
Galactinol Synthase ◽  
Coffea Arabica L
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