Sarracenia purpurea glycerol‐3‐phosphate acyltransferase 5 confers plant tolerance to high humidity in Arabidopsis thaliana

2021 ◽  
Author(s):  
Yi‐Feng Hsu ◽  
Jiawen Yan ◽  
Yu Song ◽  
Min Zheng
Keyword(s):  
Arabidopsis Thaliana ◽  
Sarracenia Purpurea ◽  
High Humidity ◽  
Plant Tolerance

scholarly journals Seed Gamma Irradiation of Arabidopsis thaliana ABA-Mutant Lines Alters Germination and Does Not Inhibit the Photosynthetic Efficiency of Juvenile Plants

Dose-Response ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 155932582097924
Author(s):  
Darya Babina ◽  
Marina Podobed ◽  
Ekaterina Bondarenko ◽  
Elizaveta Kazakova ◽  
Sofia Bitarishvili ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Growth Response ◽  
Dose Range ◽  
Fine Tuning ◽  
Plant Tolerance ◽  
Inhibitory Effects ◽  
Γ Irradiation ◽  
Photosynthetic Responses ◽  
Mutant Lines

Plant growth response to γ-irradiation includes stimulating or inhibitory effects depending on plant species, dose applied, stage of ontogeny and other factors. Previous studies showed that responses to irradiation could depend on ABA accumulation and signaling. To elucidate the role of ABA in growth and photosynthetic responses to irradiation, lines Col-8, abi3-8 and aba3 -1 of Arabidopsis thaliana were used. Seeds were γ-irradiated using 60Co in the dose range 50-150 Gy. It was revealed that the dose of 150 Gy affected germination parameters of aba3 -1 and Col-8 lines, while abi3-8 line was the most resistant to the studied doses and even showed faster germination at early hours after γ-irradiation at 50 Gy. These results suggest that susceptibility to ABA is probably more important for growth response to γ-irradiation than ABA synthesis. The photosynthetic functioning of 16-day-old plants mainly was not disturbed by γ-irradiation of seeds, and no indication of photosystem II photoinhibition was noticed, revealing the robustness of the photosynthetic system of A. thaliana. Glutathione peroxidase activity and ABA concentrations in plant tissues were not affected in the studied dose range. These results contribute to the understanding of germination and photosynthesis fine-tuning and of mechanisms of plant tolerance to ionizing radiation.

Overexpression of AtHsp90.3 in Arabidopsis thaliana impairs plant tolerance to heavy metal stress

2012 ◽  
Vol 56 (1) ◽  
pp. 197-199 ◽  
Author(s):  
H. M. Song ◽  
H. Z. Wang ◽  
X. B. Xu
Keyword(s):  
Heavy Metal ◽  
Arabidopsis Thaliana ◽  
Heavy Metal Stress ◽  
Metal Stress ◽  
Plant Tolerance

scholarly journals Over-Expression of a 14-3-3 Protein From Foxtail Millet Improves Plant Tolerance to Salinity Stress in Arabidopsis thaliana

2020 ◽  
Vol 11 ◽  
Author(s):  
Jiaming Liu ◽  
Chengyao Jiang ◽  
Lu Kang ◽  
Hongchang Zhang ◽  
Yu Song ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salinity Stress ◽  
Foxtail Millet ◽  
Plant Tolerance ◽  
Over Expression ◽  
Tolerance To Salinity

Comparison of full-length and conserved segments of wheat dehydrin DHN-5 overexpressed in Arabidopsis thaliana showed different responses to abiotic and biotic stress

2016 ◽  
Vol 43 (11) ◽  
pp. 1048 ◽  
Author(s):  
Marwa Drira ◽  
Moez Hanin ◽  
Khaled Masmoudi ◽  
Faiçal Brini
Keyword(s):  
Arabidopsis Thaliana ◽  
Fungal Infections ◽  
Alternaria Solani ◽  
Triticum Aestivum L ◽  
Full Length ◽  
Plant Tolerance ◽  
Wild Type ◽  
Biotic And Abiotic Stresses ◽  
Related Stress

Dehydrins (DHNs) are among the most common proteins accumulated in plants under water-related stress. They typically contain at least three conserved sequences designated as the Y-, S- and K-segments. The present work aims to highlight the role of the K-segments in plant tolerance to biotic and abiotic stresses. For this purpose, transgenic Arabidopsis thaliana (L.) Heyhn. lines expressing distinct wheat (Triticum aestivum L.) DHN-5 truncated constructs with or without the K-segments were generated. Our results showed that unlike the derivative lacking a K-segment, constructs containing only one or two K-segments enhanced the tolerance of A. thaliana to diverse stresses and were similar to the full-length wheat DHN-5. Moreover, compared with the wild-type and the YS form, the transgenic plants overexpressing wheat DHN-5 with K-segments maintained higher superoxide dismutase, catalase and peroxide dismutase enzymatic activity, and accumulated lower levels of H2O2 and malondialdehyde. In addition, we demonstrated that lines like A. thaliana overexpressing wheat DHN-5 showed increased resistance to fungal infections caused by Botrytis cinerea and Alternaria solani. Finally, the overexpression of the different forms of wheat DHN-5 led to the regulation of the expression of several genes involved in the jasmonic acid signalling pathway.

scholarly journals Expression, purification and crystallization of MnSOD fromArabidopsis thaliana

2014 ◽  
Vol 70 (5) ◽  
pp. 669-672 ◽  
Author(s):  
Alexandra T. Marques ◽  
Sandra P. Santos ◽  
Margarida G. Rosa ◽  
Mafalda A. A. Rodrigues ◽  
Isabel A. Abreu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Search Model ◽  
Molecular Replacement ◽  
Asymmetric Unit ◽  
Plant Tolerance ◽  
Data Set ◽  
Manganese Superoxide ◽  
Primary Antioxidant

Manganese superoxide dismutase (MnSOD) is an essential primary antioxidant enzyme. MnSOD plays an important role in plant tolerance to abiotic stress and is a target candidate for increasing stress tolerance in crop plants. Although the structure and kinetic parameters of MnSODs from several organisms have been determined, this information is still lacking for plant MnSODs. Here, recombinant MnSOD fromArabidopsis thaliana(AtMnSOD) was expressed, purified and crystallized. A nearly complete data set could only be obtained when a total rotation range of 180° was imposed during data collection, despite the seemingly tetragonal metric of the AtMnSOD crystal diffraction. The data set extended to 1.95 Å resolution and the crystal belonged to space groupP1. Molecular-replacement calculations using an ensemble of homologous SOD structures as a search model gave a unique and unambiguous solution corresponding to eight molecules in the asymmetric unit. Structural and kinetic analysis of AtMnSOD is currently being undertaken.

Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress

2009 ◽  
Vol 27 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Hongmiao Song ◽  
Pengxiang Fan ◽  
Yinxin Li
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Plant Tolerance

scholarly journals Clinorotation Affects Induction of the Heat Shock Response in Arabidopsis thaliana Seedlings

2020 ◽  
Vol 6 (1) ◽  
pp. 2-9
Author(s):  
Liudmyla Y. Kozeko ◽  
Denis D. Buy ◽  
Yaroslav V. Pirko ◽  
Yaroslav B. Blume ◽  
Elizaveta L. Kordyum
Keyword(s):  
Heat Treatment ◽  
Arabidopsis Thaliana ◽  
Heat Shock ◽  
Seedling Survival ◽  
Transcript Abundance ◽  
Mild Stress ◽  
Plant Tolerance ◽  
Stationary Conditions ◽  
Kinetics Of ◽  
Better Than

AbstractClinorotation used to simulate microgravity effects in ground-based experiments is considered as a mild stress factor for plants. We have assumed that it might influence the plant tolerance to other stressful factors. To test this, Arabidopsis thaliana seedlings were grown on a horizontal clinostat (2 rpm) or under stationary conditions (control), and then were subjected to heat treatment. The kinetics of gene expression of cytosolic HSP70s and HSP90s during exposure to 37°C for 0.5-2 h was examined by RT-qPCR to estimate level of the heat shock reaction. It was shown that clinorotation caused the minor increase in transcript abundance of five AtHSP70s and AtHSP90-1 under normal temperature, as well as a faster onset and enhancement of their induction during heat shock. The heat tolerance was evaluated as a function of seedling survival after exposure to 45°C for 45 min. Seedlings grown under clinorotation were determined to withstand heat treatment better than seedlings grown under stationary conditions. The obtained data support the assumption that clinorotation may provide cross-protection of plants against fluctuations in environmental conditions.

scholarly journals Molecular Evolution and Functional Analysis of Rubredoxin-Like Proteins in Plants

2019 ◽  
Vol 2019 ◽  
pp. 1-14
Author(s):  
Ying Li ◽  
Pan pan Liu ◽  
Xin Ni
Keyword(s):  
Arabidopsis Thaliana ◽  
Higher Plants ◽  
Expression Patterns ◽  
Critical Role ◽  
Anaerobic Bacteria ◽  
Plant Tolerance ◽  
Pdz Domains ◽  
Biochemical Processes ◽  
Molecular Weights ◽  
Relationship Of

Rubredoxins are a class of iron-containing proteins that play an important role in the reduction of superoxide in some anaerobic bacteria and also act as electron carriers in many biochemical processes. Unlike the more widely studied about rubredoxin proteins in anaerobic bacteria, very few researches about the function of rubredoxins have been proceeded in plants. Previous studies indicated that rubredoxins inA. thalianamay play a critical role in responding to oxidative stress. In order to identify more rubredoxins in plants that maybe have similar functions as the rubredoxin-like protein ofA. thaliana, we identified and analyzed plant rubredoxin proteins using bioinformatics-based methods. Totally, 66 candidate rubredoxin proteins were identified based on public databases, exhibiting lengths of 187–360 amino acids with molecular weights of 19.856–37.117 kDa. The results of subcellular localization showed that these candidate rubredoxins were localized to the chloroplast, which might be consistent with the fact that rubredoxins were predominantly expressed in leaves. Analyses of conserved motifs indicated that these candidate rubredoxins contained rubredoxin and PDZ domains. The expression patterns of rubredoxins in glycophyte and halophytic plant under salt/drought stress revealed that rubredoxin is one of the important stress response proteins. Finally, the coexpression network of rubredoxin inArabidopsis thalianaunder abiotic was extracted from ATTED-II to explore the function and regulation relationship of rubredoxin inArabidopsis thaliana. Our results showed that putative rubredoxin proteins containing PDZ and rubredoxin domains, localized to the chloroplast, may act with other proteins in chloroplast to responses to abiotic stress in higher plants. These findings might provide value inference to promote the development of plant tolerance to some abiotic stresses and other economically important crops.

scholarly journals Exogenous Stilbenes Improved Tolerance of Arabidopsis thaliana to a Shock of Ultraviolet B Radiation

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1282
Author(s):  
Zlata V. Ogneva ◽  
Vlada V. Volkonskaia ◽  
Alexandra S. Dubrovina ◽  
Andrey R. Suprun ◽  
Olga A. Aleynova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Crop Production ◽  
Foliar Application ◽  
Survival Rates ◽  
Ultraviolet B ◽  
Plant Phenolics ◽  
Plant Tolerance ◽  
Positive Role ◽  
Irradiation Intensity ◽  
Protective Shield

Excessive ultraviolet B (UV-B) irradiation is one of the most serious threats leading to severe crop production losses. It is known that secondary metabolite biosynthesis plays an important role in plant defense and forms a protective shield against excessive UV-B irradiation. The contents of stilbenes and other plant phenolics are known to sharply increase after UV-B irradiation, but there is little direct evidence for the involvement of stilbenes and other plant phenolics in plant UV-B protection. This study showed that foliar application of trans-resveratrol (1 and 5 mM) and trans-piceid (5 mM) considerably increased tolerance to a shock of UV-B (10 min at 1800 µW cm−2 of irradiation intensity) of four-week-old Arabidopsis thaliana plants that are naturally incapable of stilbene production. Application of trans-resveratrol and trans-piceid increased the leaf survival rates by 1–2%. This stilbene-induced improvement in UV-B tolerance was higher than after foliar application of the stilbene precursors, p-coumaric and trans-cinnamic acids (only 1–3%), but less than that after treatment with octocrylene (19–24%), a widely used UV-B absorber. Plant treatment with trans-resveratrol increased expression of antioxidant and stress-inducible genes in A.thaliana plants and decreased expression of DNA repair genes. This study directly demonstrates an important positive role of stilbenes in plant tolerance to excessive UV-B irradiation, and offers a new approach for plant UV-B protection.

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