A Study of Enthalpic Relaxation of Trehalose-Water Glasses

2004 ◽  
Author(s):  
James Green ◽  
Alex Fowler ◽  
Mehmet Toner ◽  
Sankha Bhowmick
Keyword(s):  
Molecular Mobility ◽  
Physical Aging ◽  
Glassy Matrix ◽  
Relaxation Kinetics ◽  
Ambient Temperatures ◽  
Enthalpic Relaxation ◽  
Long Term Storage ◽  
And Storage ◽  
Kinetics Of

Trehalose has been shown to be an effective biostabilizer, one explanation for this is that it can easily form glass at near ambient temperatures. Enthalpic relaxation is a physical aging process that occurs in glasses held near, but below, their Tg and is an indicator of the amount of molecular mobility of a particular glass. Since molecular mobility is key to long-term storage biologics in a glassy matrix this study was developed to gain a fundamental understanding of the enthalpic relaxation kinetics of trehalose-water glasses. Through the use of DSC, enthalpic peaks were quantitatively measured for water-trehalose glasses as a function of aging time and aging temperature for three different mass percentages (Mwater/Mtrehalose%, ~0%, ~1.5%, & ~10%). From this data the total amount of relaxation and the relaxation time have been determined using the Cowie-Ferguson equation. Understanding the effects of water on enthalpic relaxation kinetics will help lead to optimized drying protocols and storage parameters.

MODERN PROBLEMATICS AND ANALYSIS OF EFFICIENCY OF APPLICATION OF DIFFERENT APPROACHES TO DESTRUCTION OF INFORMATION ON MAGNETIC MEDIA

2019 ◽  
Vol 2 (12) ◽  
pp. 154-164
Author(s):  
O. Semenenko ◽  
O. Vodchyts ◽  
V. Koverga ◽  
R. Lukash ◽  
O. Lutsenko
Keyword(s):  
Armed Forces ◽  
Information Storage ◽  
Storage Device ◽  
Magnetic Media ◽  
Long Term Storage ◽  
Physical Impact ◽  
And Storage ◽  
Term Storage

The introduction and active use of information transmission and storage systems in the Ministry of Defense (MoD) of Ukraine form the need to develop ways of guaranteed removal of data from media after their use or long-term storage. Such a task is an essential component of the functioning of any information security system. The article analyzes the problems of guaranteed destruction of information on magnetic media. An overview of approaches to the guaranteed destruction of information on magnetic media of different types is presented, and partial estimates of the effectiveness of their application are given by some generally accepted indicators of performance evaluation. The article also describes the classification of methods of destruction of information depending on the influence on its medium. The results of the analysis revealed the main problems of application of software methods and methods of demagnetization of the information carrier. The issue of guaranteed destruction of information from modern SSD devices, which are actively used in the formation of new systems of information accumulation and processing, became particularly relevant in the article. In today's conditions of development of the Armed Forces of Ukraine, methods of mechanical and thermal destruction are more commonly used today. In the medium term, the vector of the use of information elimination methods will change towards the methods of physical impact by the pulsed magnetic field and the software methods that allow to store the information storage device, but this today requires specialists to develop new ways of protecting information in order to avoid its leakage.

scholarly journals Impact of long-term storage at ambient temperatures on the total quality and stability of high-pressure processed tomato juice

2015 ◽  
Vol 32 ◽  
pp. 1-8 ◽  
Author(s):  
K.G.L.R. Jayathunge ◽  
Irene R. Grant ◽  
Mark Linton ◽  
Margaret F. Patterson ◽  
Anastasios Koidis
Keyword(s):  
High Pressure ◽  
Tomato Juice ◽  
Total Quality ◽  
Ambient Temperatures ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Chlorogenic acid content in potato tubers with colored flesh as affected by a genotype, location and long-term storage

2019 ◽  
Vol 65 (No. 7) ◽  
pp. 355-360
Author(s):  
Matyáš Orsák ◽  
Karel Hamouz ◽  
Jaromír Lachman ◽  
Pavel Kasal
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
Field Experiments ◽  
The Other ◽  
Potato Tubers ◽  
Flesh Color ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

In three-year field experiments, the effect of genotype, flesh color, site conditions and storage on chlorogenic acid content (CAC) in tubers of potato cultivars with purple or red flesh was compared to yellow-fleshed cv. Agria. The results confirmed the significant effect of genotype on CAC. The highest CAC was characteristic on a three-year mean for the purple-fleshed cv. Vitelotte (769.5 mg/kg fresh weight (FW)), i.e. 1.19−2.6 times higher than in the other cultivars. In regard to the effect of flesh color, significantly higher mean CAC levels have been shown for the red-fleshed (2.8 times) and purple-fleshed (3.16 times) cultivars in comparison with cv. Agria (148 mg/kg FW). At the Uhříněves location with a warmer climate and frequent dry periods as compared to the second Valečov location, a higher CAC (1.18 times) was found. Cold storage (4°C, 6 months) resulted in a significant CAC increase varying from 33.2% in the Blaue St. Galler cultivar to 210.6% in the Vitelotte cultivar among all eight evaluated color-fleshed cultivars. On the other hand, the effect of storage on CAC was not evident in the yellow-fleshed Agria cultivar (inconclusive difference against CAC after harvest).

scholarly journals Aboveground storage of hydrocarbon gas hydrates

2018 ◽  
Vol 60 ◽  
pp. 00028
Author(s):  
Mykola Zotsenko ◽  
Larysa Pedchenko ◽  
Andrii Manhura
Keyword(s):  
Gas Hydrate ◽  
Mechanical Characteristics ◽  
Negative Temperature ◽  
Prolonged Storage ◽  
Long Term Storage ◽  
Hydrocarbon Gas ◽  
Mixing Method ◽  
And Storage ◽  
Term Storage

The work objective is to substantiate the technical bases for the production of gas hydrate blocks directly on-site for further transportation and storage in above ground storages, as an alternative to transporting and storing gas in underground gas storages. The theoretical bases have been considered and processes of gas hydrate blocks producing, physical-mechanical characteristics of artificial hydrates with the purpose of determining the conditions of their storage and prolonged storage were tested in laboratory conditions. The construction of above ground gas hydrate storage in the form of a building, partially deepened in a ground, which is separated from the environment by a wall of gravel cement elements made by the mixing method were substantiated. It is proved that such a constructive solution for a long-term storage of gas hydrate blocks is the most economical in terms of energy consumption to maintain internal negative temperature.

Nutrient losses in cereal and pasture hays stored in three types of hay packages in the open in two environments

1985 ◽  
Vol 25 (2) ◽  
pp. 326 ◽  
Author(s):  
MJ Cochrane ◽  
RB Wickes
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Losses ◽  
Storage Medium ◽  
Long Term Storage ◽  
Large Round ◽  
And Storage ◽  
Term Storage ◽  
Lower Medium

Cereal and pasture hays, cut at the same site, were packaged into medium-sized round bales, large round bales and stacks. Halfthe number of each hay type and of each hay package were stored in the open for up to 15 months in a 500 mm rainfall area and half were transported to and stored for up to 15 months in a 1000 mm rainfall area. Densities of the pasture hay packages were within the manufacturers' specified ranges but those of the cereal hay packages were lower. Medium-sized round bales were less dense than large round bales and weathered more than large round bales and stacks after 10 and 15 months storage. Cereal hay packages deteriorated more than pasture hay packages with long-term storage. Medium-sized round bales lost more dry matter than large round bales after storage over the winter and more digestible dry matter over this period and after long-term storage. They also lost more crude protein than large round bales when stored over the first summer and over the following winter. It was economic to store all packages in the open over the first summer. It was uneconomic to store medium-sized round bales in the open over the following winter, while storage of large round bales and stacks was economic. For long-term storage, it was uneconomic to store any of the hay packages in the open, and storage under cover would have to be considered.

Relationship between Acoustic Firmness and Magness Taylor Firmness in Royal Gala and Golden Smoothee Apples

2009 ◽  
Vol 15 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. Molina-Delgado ◽  
S. Alegre ◽  
J. Puy ◽  
I. Recasens
Keyword(s):  
Quality Parameters ◽  
Acoustic Measurements ◽  
Good Tool ◽  
Malus Domestica Borkh ◽  
Long Term Storage ◽  
Acoustic Methods ◽  
The Relationship ◽  
And Storage ◽  
Term Storage

This study aimed to evaluate apple firmness measured using both the penetrometer and acoustic methods. The methodologies were applied to Royal Gaya and Golden Smoothee apples (Malus domestica. Borkh) harvested from 12 different orchards in Catalonia (Spain), on six different dates, and over three seasons. The relationship between firmness and physicochemical quality parameters was analyzed at harvest and post-harvest. The results obtained showed a noticeable correlation between Magness Taylor firmness and acoustic measurements in Royal Gala, but no correlation was found for Golden Smoothee. Fruit variety and storage had a clear influence on correlations between the two measurements. Measurements of Magnes Taylor and acoustic firmness seemed to correspond to different physical properties. Acoustic measurements seemed to be a good tool for evaluating changes in tissue firmness during long-term storage, especially for Golden Smoothee apples. The maturity of the fruit seemed to play a more important role in the measurement of Magnes Taylor firmness than in the measurement of acoustic firmness for the Royal Gaya variety.

scholarly journals An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot

2002 ◽  
Vol 35 (6) ◽  
pp. 720-726 ◽  
Author(s):  
Aina E. Cohen ◽  
Paul J. Ellis ◽  
Mitchell D. Miller ◽  
Ashley M. Deacon ◽  
R. Paul Phizackerley
Keyword(s):  
Synchrotron Radiation ◽  
Industrial Robot ◽  
Automated System ◽  
Small Scale ◽  
Compact Sample ◽  
Long Term Storage ◽  
Radiation Laboratory ◽  
And Storage ◽  
Term Storage

An automated system for mounting and dismounting pre-frozen crystals has been implemented at the Stanford Synchrotron Radiation Laboratory (SSRL). It is based on a small industrial robot and compact cylindrical cassettes, each holding up to 96 crystals mounted on Hampton Research sample pins. For easy shipping and storage, the cassette fits inside several popular dry-shippers and long-term storage Dewars. A dispensing Dewar holds up to three cassettes in liquid nitrogen adjacent to the beamline goniometer. The robot uses a permanent magnet tool to extract samples from, and insert samples into a cassette, and a cryo-tong tool to transfer them to and from the beamline goniometer. The system is simple, with few moving parts, reliable in operation and convenient to use.

scholarly journals Impact of Freeze-thaw Cycles and Storage Time on Plasma Samples Used in Mass Spectrometry Based Biomarker Discovery Projects

2005 ◽  
Vol 1 ◽  
pp. 117693510500100 ◽  
Author(s):  
Breeana L Mitchell ◽  
Yutaka Yasui ◽  
Christopher I Li ◽  
Annette L. Fitzpatrick ◽  
Paul D Lampe
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Plasma Proteome ◽  
Sample Handling ◽  
Freeze Thaw ◽  
Long Term Storage ◽  
And Storage ◽  
Human Fluids ◽  
Term Storage

Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches have been widely investigated, little attention has been paid to how sample handling can impact the plasma proteome and therefore influence biomarker discovery. We have investigated the effects of two main aspects of sample handling on MALDI-TOF data: repeated freeze-thaw cycles and the effects of long-term storage of plasma at –70°C. Repeated freeze-thaw cycles resulted in a trend towards increasing changes in peak intensity, particularly after two thaws. However, a 4-year difference in long-term storage appears to have minimal effect on protein in plasma as no differences in peak number, mass distribution, or coefficient of variation were found between samples. Therefore, limiting freeze/thaw cycles seems more important to maintaining the integrity of the plasma proteome than degradation caused by long-term storage at –70°C.

Sign in / Sign up

Export Citation Format

Share Document