Fabrication of Porous Hydroxyapatite Scaffolds

2006 ◽  
Author(s):  
S. Deville ◽  
P. Miranda ◽  
E. Saiz ◽  
A. P. Tomsia
Keyword(s):  
Tissue Engineering ◽  
Porous Materials ◽  
Three Dimensional ◽  
Ceramic Materials ◽  
Ice Crystals ◽  
Freeze Casting ◽  
Porous Hydroxyapatite ◽  
Preliminary Results ◽  
Calcified Tissue ◽  
Ceramic Suspensions

This work describes two novel techniques for the fabrication of porous hydroxyapatite scaffolds for calcified tissue engineering; robocasting and freeze casting. These techniques allow the fabrication of materials with complex porosity. Both are based on the preparation of concentrated ceramic suspensions with suitable properties for the process. In robocasting, the computer-guided deposition of the suspensions is used to build porous materials with designed three dimensional (3-D) geometries and microstructures. Freeze casting uses ice crystals as a template to form porous lamellar ceramic materials. Preliminary results on the compressive strengths of the materials are also reported.

Bioscaffolds in Periodontal Regeneration

2019 ◽  
Vol 9 (4) ◽  
pp. 428-436
Author(s):  
Jothi Varghese ◽  
Rudra Mohan
Keyword(s):  
Tissue Engineering ◽  
Research Work ◽  
Periodontal Regeneration ◽  
Three Dimensional ◽  
Ceramic Materials ◽  
Modern Technology ◽  
Cellular Growth ◽  
Bone Tissue Regeneration ◽  
Biomedical Science ◽  
Periodontal Tissues

Background: Tissue engineering is a highly evolving field in periodontology which incorporates the use of cells, signalling molecules and scaffolds thereby creating a three dimensional microenvironment facilitating cellular growth and function for restoration of lost tissues due to periodontal disease. This review discusses the various types, ideal characteristics, properties and applications of potential scaffolds that can be used in periodontal regeneration with the help of principles of tissue engineering. Methods: Research work pertaining to bioscaffolds for periodontal regeneration were selected using key words in major databases and internet sources. Results: Studies related to various features of scaffold and its inherent properties were searched and analysed. Data were organized considering the sources of its origin and salient features of these inert matrices. Specific probe into the techniques and medium used for developing scaffolds were cited. Further, bioactive ceramic materials which are involved in stimulating cell proliferation, and bone tissue regeneration, which may also facilitate periodontal regeneration were mentioned. Likewise, few data linked to different types of biodegradable synthetic scaffolds and its advantages were considered. The progress of science in various fabrication techniques and newer advances using modern technology such as tissue engineering approaches, 3D printing and physical & chemical methods to enhance the physical properties are being used to make them more versatile for the application in the field of biomedical science. Conclusion: In lieu of the available literature search and vast progress in material science, scaffolds construction for cellular regeneration requires wide exploration. Furthermore, when these scaffolds are placed at a particular site, it should be able to restore lost periodontal tissue. Also, the newer innovative technologies like the 3D version of biomimicking, nano/micro-based scaffolds displays potential for further extensive research and complete regeneration of periodontal tissues.

Hierarchical porous materials for tissue engineering

2005 ◽  
Vol 364 (1838) ◽  
pp. 263-281 ◽  
Author(s):  
Julian R Jones ◽  
Peter D Lee ◽  
Larry L Hench
Keyword(s):  
Tissue Engineering ◽  
Porous Materials ◽  
Materials Science ◽  
Three Dimensional ◽  
Hierarchical Structures ◽  
Engineering Applications ◽  
Culture Techniques ◽  
Tissue Replacement ◽  
Hierarchical Porous

Biological organisms have evolved to produce hierarchical three-dimensional structures with dimensions ranging from nanometres to metres. Replicating these complex living hierarchical structures for the purpose of repair or replacement of degenerating tissues is one of the great challenges of chemistry, physics, biology and materials science. This paper describes how the use of hierarchical porous materials in tissue engineering applications has the potential to shift treatments from tissue replacement to tissue regeneration. The criteria that a porous material must fulfil to be considered ideal for bone tissue engineering applications are listed. Bioactive glass foam scaffolds have the potential to fulfil all the criteria, as they have a hierarchical porous structure similar to that of trabecular bone, they can bond to bone and soft tissue and they release silicon and calcium ions that have been found to up-regulate seven families of genes in osteogenic cells. Their hierarchical structure can be tailored for the required rate of tissue bonding, resorption and delivery of dissolution products. This paper describes how the structure and properties of the scaffolds are being optimized with respect to cell response and that tissue culture techniques must be optimized to enable growth of new bone in vitro .

One pot method to synthesize three-dimensional porous hydroxyapatite nanocomposite for bone tissue engineering

2019 ◽  
Vol 27 (1) ◽  
pp. 225-235 ◽  
Author(s):  
Chandrani Sarkar ◽  
Kumar Anuvrat ◽  
Subhadra Garai ◽  
Sumanta Kumar Sahu ◽  
Jui Chakraborty
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
One Pot ◽  
Porous Hydroxyapatite

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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