scholarly journals Studies on the Establishment of a Measles Antibodies Detection Method Using Enzyme-Linked Immunosorbent Assay (ELISA)

1988 ◽  
Vol 62 (12) ◽  
pp. 1157-1165
Author(s):  
Tetsuya SHIMAZAKI
Keyword(s):  
Immunosorbent Assay ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Antibodies ◽  
Antibodies Detection

Measles antibodies and response to vaccination in children aged less than 14 months: implications for age of vaccination

2011 ◽  
Vol 140 (9) ◽  
pp. 1599-1606 ◽  
Author(s):  
E. BORRÀS ◽  
L. URBIZTONDO ◽  
J. COSTA ◽  
J. BATALLA ◽  
N. TORNER ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Maternal Antibodies ◽  
Passive Immunity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Blood Samples ◽  
Measles Outbreak ◽  
Measles Antibodies ◽  
Measles Vaccination

SUMMARYPassive immunity against measles decreases during the first months of life. The objective of this study was to determine titres of measles antibodies in children aged 9–14 months and their mothers before vaccination, and the children's response to vaccination. Blood samples were collected by capillary puncture before and 28 days after vaccination. Samples were obtained between February and June 2007 during an ongoing measles outbreak. Titres of specific measles IgG antibodies were determined by enzyme-linked immunosorbent assay. Seroconversion was defined as the presence of antibodies after vaccination in subjects without antibodies before vaccination. Maternal antibodies were present in 37·7% of all 69 children included and in 45·1% of children aged 9 months. Of the 51 children in whom a second sample was obtained, 31 (60·8%) were seronegative before vaccination and 61·3% seroconverted. Interference of maternal antibodies was 30%. Advancing the first dose of measles vaccination from 15 to 12 months is a correct strategy, given the increase in the time of susceptibility of infants to measles.

scholarly journals Levels of Airborne Soybean Allergen (Gly m 1) in a Brazilian Soybean Production City: A Pilot Study

2020 ◽  
Vol 17 (15) ◽  
pp. 5381
Author(s):  
Cinthya Covessi Thom de Souza ◽  
Nelson Augusto Rosário Filho ◽  
Juliana Francis de Camargo ◽  
Ricardo Henrique Moreton Godoi
Keyword(s):  
Pilot Study ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soybean Production ◽  
Soy Production ◽  
Assay Detection ◽  
Soybean Allergen

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

scholarly journals Detection of Antigenemia by Enzyme-Linked Immunosorbent Assay in Horses with Experimental Ehrlichia Risticii Infection

1993 ◽  
Vol 5 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Richard E. Corstvet ◽  
Stephen D. Gaunt ◽  
Phillip A. Karns ◽  
David Burgermeister ◽  
Jere W. McBride ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Horse ◽  
Ehrlichia Risticii

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti- E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

scholarly journals Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

1991 ◽  
Vol 33 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Vanda Akico Ueda Fick de Souza ◽  
Claudio Sergio Pannuti ◽  
Laura Massami Sumita ◽  
Paul Albrecht
Keyword(s):  
Umbilical Cord ◽  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
Haemagglutination Inhibition ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Reproducibility ◽  
Measles Antibodies

An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copo-sitivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.

Prevalence of Measles Antibodies in Hospital Personnel

1986 ◽  
Vol 7 (6) ◽  
pp. 309-311 ◽  
Author(s):  
Teresa Chou ◽  
Diane Weil ◽  
Paul M. Arnow
Keyword(s):  
Young Adults ◽  
Immunosorbent Assay ◽  
Hemagglutination Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hospital Personnel ◽  
Testing Methods ◽  
Immunization Program ◽  
Negative Results ◽  
Measles Antibodies ◽  
Low Rate

AbstractA community outbreak of measles prompted a serologic survey of personnel in a hospital serving that community. Two hundred sixty-six personnel, primarily physicians (129) and nurses (100), voluntarily participated. Serum specimens were initially tested by the immunofluorescent antibody and enzyme-linked immunosorbent assay (EIA) methods. Specimens with negative results by either test were further examined using the hemagglutination inhibition and plaque neutralization (PN) methods. If EIA and PN results were negative, an individual was considered susceptible. Only one of the 98 participants born during or after 1957 and none of the 168 participants born before 1957 were serosusceptible. The low rate of serosusceptibility, in contrast to previous studies of young adults, appears attributable to the sensitivity of the testing methods used. Based on our experience, institutions considering a measles serologic testing and immunization program should expect to identify very few serosusceptible personnel, even among those born during or after 1957.

scholarly journals Produksi imunoglobulin Y (IgY) untuk pengembangan metode deteksi dini kontaminasi okratoksin (Immunoglobulin Y (IgY) production to develop an early detection method for ochratoxin contamination)

2018 ◽  
Vol 86 (1) ◽  
Author(s):  
Irma KRESNAWATY ◽  
. SUHARYANTO ◽  
. SISWANTO ◽  
Sumi HUDIYONO
Keyword(s):  
Early Detection ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Polyclonal Antibodies ◽  
Egg Laying ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dot Blot ◽  
Negative Effects ◽  
Sds Page

Indonesian coffee and cocoa commodities are constrained by low product quality problem due to contamination of fungal metabolites which  producing ochratoxin A (OTA). Ochratoxin is neprotoxic, immunogenic, carcinogenic and teratogenic to the human health. Early detection method on site detection should be developed  because of  those negative effects. The aim of this study  was to produce antibody to develop a method for  OTA detection. Antibody was produced by immunization of egg laying hen. Antibody-produced was sepatared and analyzed using ELISA (Enzyme-Linked Immunosorbent Assay) and DBIA (dot blot immunoassay),and tested its composition using HPLC and SDS PAGE. The results showed that anti-OTA polyclonal antibodies had been obtained already from chicken eggs in the 4th period (7 weeks after initial immunization). These antibodies showed anti-OTA reactivity by DBIA method and still showed anti-OTA reactivity up to 9th period (12 weeks after initial immunization). The anti-BSA antibodies produced should be removed to increase the sensitivity of antibodies againts ochratoxin A. The separation of BSA antibodies can be conducted by the absorption of the protein.  [Keywords: ochratoxin A; early detection; antibody IgY]. AbstrakKomoditas kopi dan kakao Indonesia terkendala masalah mutu produk yang rendah akibat kontaminasi cendawan penghasil okratoksin A. Okratoksin A (OTA) bersifat neprotoksik, imunogenik, karsinogenik dan teratogenik yang membahayakan kesehatan manusia. Karena efek negatif yang diakibatkan oleh mikotoksin ini, maka perlu dikembangkan deteksi dini kontaminasi okratoksin langsung di lokasi. Penelitian ini bertujuan menghasilkan antibodi imunoglobulin Y (IgY) untuk mengembangkan metode perakitan perangkat deteksi cepat berbasis imunologi untuk deteksi OTA. Antibodi dihasilkan menggunakan uji ayam petelur. Antibodi yang dihasilkan dipisahkan dan dianalisis aktivitasnya dengan ELISA (Enzyme-Linked Immunosorbent Assay) dan DBIA (dot blot immunoassay), serta diuji komposisinya dengan HPLC dan SDS PAGE. Hasil penelitian menunjukkan bahwa antibodi poliklonal anti-OTA sudah diperoleh dari telur ayam pada periode ke-4 (7 minggu setelah imunisasi awal). Antibodi ini menunjukkan reaktivitas anti-OTA dengan metode DBIA dan masih menunjukkan reaktivitas anti-OTA sampai periode 9 (12 minggu setelah imunisasi awal). Komposisi asam amino antibodi anti-OTA menunjukkan perbedaan dengan komposisi asam amino IgY di database. Antibodi anti BSA yang dihasilkan harus dihilangkan terlebih dahulu untuk meningkatkan sensitivitas antibodi terhadap okratoksin A dan pemisahan dapat dilakukan dengan penyerapan antibodi BSA.[Kata Kunci:  okratoksin A;  deteksi dini; antibodi IgY].

scholarly journals Characteristics of measles immunity in students of the pediatric faculty of a medical university

2020 ◽  
Vol 12 (4) ◽  
pp. 58-64
Author(s):  
D. O. Ivanov ◽  
V. N. Timchenko ◽  
E. B. Pavlova ◽  
E. V. Barakina ◽  
T. M. Chernova ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Measles Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Vaccine ◽  
Specific Antibodies ◽  
Measles Antibodies ◽  
Measles Vaccination ◽  
Instructions For Use ◽  
Medical University ◽  
The Relationship

Objective: to evaluate the intensity of measles immunity in students of I–VI courses of the pediatric faculty of St. Petersburg State Medical University.Materials and methods: titers of antibodies to measles virus were determined in 986 students of I–VI courses in May –June 2019. The level of measles antibodies was determined by enzyme-linked immunosorbent assay.Results: positive (protective) titres of measles antibodies were detected in 555 people. (56.4%), the absence of measles immunity was found in 431 people. (43.6%), including 57 people. (5.8%) specific antibodies were not detected. The relationship between the level of collective immunity and the duration of the period after the last vaccination against measles was revealed.Conclusions: in the majority of examined students (96.2%), protective titers of measles antibodies remained for 10 years after the last vaccination. Subsequently, a significant decrease in the level of measles antibodies was noted. All students who do not have a protective IgG titer are shown measles vaccination with live measles vaccine in accordance with the instructions for use. 

A Portable Fiberoptic Monitor for Fluorimetric Bioassays

1986 ◽  
Vol 40 (5) ◽  
pp. 696-700 ◽  
Author(s):  
T. Vo-Dinh ◽  
G. D. Griffin ◽  
K. R. Ambrose
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzyme Substrate ◽  
Rabbit Immunoglobulin ◽  
Conventional Elisa ◽  
Better Than

A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiberoptic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 μg. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay is three orders of magnitude better than that obtained with a conventional ELISA absorption spectrometric assay using a commercially available reader.

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