Erratum: “Early-stage corrosion, ion release, and the antibacterial effect of copper and cuprous oxide in physiological buffers: Phosphate-buffered saline vs Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid” [Biointerphases 14, 061004 (2019)]

Abstract Infection and insufficient osteointegration are the main causes of orthopedic implant failure. Furthermore, activating favorable inflammation response is vital to the fast osteointegration of implants. Therefore, endowing the implants with multifunctions (antibacterial, anti-inflammation, and pro-osteointegration) is a promising strategy to improve the performance of orthopedic implants. In this study, a Zn-contained polydopamine (PDA) film was fabricated on AZ31 alloy. The film possessed a stable Zn ion release in 14 days. The results of electrochemical analysis implied the favorable corrosion protection of the film, and thus, leading to a suitable hemolysis ratio (below 1%). The in vitro antibacterial assessment revealed that the film exhibited excellent resistance against Staphylococcus aureus (nearly 100%), which can be ascribed to the release of Zn ions. The cell-culture evaluation revealed that the extract of Zn-contained PDA-coated sample can activate RAW264.7 polarization to an anti-inflammatory phenotype, as well as enhance the osteogenic differentiation ability of MC3T3-E1. Additionally, the femoral osteomyelitis model indicated that the as-prepared film had a high antibacterial capability at early stage of the implantation, and showed better osteogenesis and osteointegration after 8 weeks of implantation. With favorable antibacterial, anti-inflammation, and pro-osteogenesis abilities, the novel designed Zn-contained PDA film is promising to be used in Mg-based orthopedic implants.

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Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide

Veterinární Medicína ◽

10.17221/1991-vetmed ◽

2008 ◽

Vol 53 (No. 7) ◽

pp. 347-357 ◽

Cited By ~ 4

Author(s):

Z. Sladek ◽

D. Rysanek

Keyword(s):

Inflammatory Response ◽

Inflammatory Responses ◽

Early Stage ◽

Muramyl Dipeptide ◽

Cell Suspensions ◽

The Other ◽

Total Count ◽

The Difference ◽

Phosphate Buffered Saline ◽

Time Point

The aim of this study was to determine whether expression of CD14 on macrophages is regulated differently during initiation and resolution of the inflammatory response caused by CD14-dependent (lipopolysaccharide) and CD14-independent (muramyldipeptide) bacterial signals. In cell suspensions from the site of inflammation we observed two types of macrophages: non-vacuolized (NMAC) and vacuolized (VMAC) cells. NMAC (monocyte-like cells) were dominant during the early stage of the inflammatory response, whilst VMAC contained phagocytosed apoptotic neutrophils in various stages of digestion. These latter cells were dominant during resolution (particularly at the last time point of 168 h). Intramammary instillation of muramyldipeptide (MDP) and lipopolysaccharide (LPS) resulted in a significant increase in the total count of CD14+ NMAC after 24 h (muramyldipeptide P < 0.01 and lipopolysaccharide P < 0.05) compared to phosphate buffered saline (PBS). During resolution of the inflammatory response, a gradual decrease in the total count of CD14+ NMAC was observed. The difference compared with PBS was significant at 48 h and 72 h after instillation of both bacterial agents (muramyldipeptide: P < 0.05; lipopolysaccharide: P < 0.05). A lower total count of CD14+ VMAC was observed as an effect of MDP and LPS at 24 h after induction (P < 0.05), when compared to PBS. During resolution, the total count of CD14+ VMAC increased. Differences (P < 0.01) were observed at 72 h and 168 h after LPS compared to PBS. We therefore assume that the expression of CD14 on macrophages is not regulated differently during the inflammatory responses caused by CD14-dependent and CD14-independent bacterial signals. On the other hand, the stage of the inflammatory response to MDP and LPS played an important role in the regulation of CD14 expression on macrophages.

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Can Copper Be Used to Treat Foliar Phytophthora Infections in Pinus radiata?

Plant Disease ◽

10.1094/pdis-07-18-1247-re ◽

2019 ◽

Vol 103 (8) ◽

pp. 1828-1834 ◽

Cited By ~ 1

Author(s):

Carol Rolando ◽

Chanatda Somchit ◽

Martin K-F. Bader ◽

Stuart Fraser ◽

Nari Williams

Keyword(s):

Pinus Radiata ◽

Cuprous Oxide ◽

Treatment Efficacy ◽

Copper Content ◽

Foliar Spray ◽

Control Treatment ◽

Needle Cast ◽

Potted Plants ◽

Red Needle Cast ◽

Effect Of Copper

Red needle cast is a significant foliar disease of commercial stands of Pinus radiata caused by Phytophthora pluvialis in New Zealand. The effect of copper, applied as a foliar spray of cuprous oxide at a range of doses between 0 and 1.72 kg ha−1, was investigated in two controlled trials with potted plants and in an operational trial with mature P. radiata. In all trials, lesions formed on needles after artificial exposure to the infecting propagules (zoospores) of P. pluvialis were used to determine treatment efficacy, with the number and/or length of lesions as the dependent variable. Results across all trials indicated that cuprous oxide was highly effective at reducing infection of P. radiata with P. pluvialis. Application rates equivalent to ≥0.65 kg ha−1 significantly reduced infection levels relative to a control treatment, with foliar surface copper levels as low as 13 to 26 mg kg−1 of needle tissue preventing infection. Greater copper content was associated with a reduction in the proportion of needles with P. pluvialis lesions, with the probability of lesions developing decreasing approximately 1% for every 1 unit (in milligrams per kilogram) increase in copper content. Over a 90-day period, surface copper content declined to 30% of that originally applied, indicating an approximate period of treatment efficacy of 3 months. Our findings highlight the potential of cuprous oxide for the control of red needle cast in P. radiata stands. Further information about the optimal field dose, timing, and the frequency of foliar cuprous oxide application is key to prevent infection and also reduce the build up of inoculum during severe outbreaks of this pathogen.

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Antibacterial Effect of Copper Sulfide Nanoparticles on Infected Wound Healing

Surgical Infections ◽

10.1089/sur.2020.411 ◽

2021 ◽

Author(s):

Yuan Liang ◽

Jiale Zhang ◽

Huahong Quan ◽

Pei Zhang ◽

Keteng Xu ◽

...

Keyword(s):

Wound Healing ◽

Copper Sulfide ◽

Antibacterial Effect ◽

Infected Wound ◽

Effect Of Copper

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Size- and Morphology-Dependent Antibacterial Properties of Cuprous Oxide Nanoparticle and Their Synergistic Antibacterial Effect

Science of Advanced Materials ◽

10.1166/sam.2016.2696 ◽

2016 ◽

Vol 8 (5) ◽

pp. 1074-1078 ◽

Cited By ~ 3

Author(s):

Boxi She ◽

Xuejuan Wan ◽

Jiaoning Tang ◽

Yuanming Deng ◽

Xiaofeng Zhou ◽

...

Keyword(s):

Cuprous Oxide ◽

Antibacterial Effect ◽

Antibacterial Properties ◽

Oxide Nanoparticle ◽

Size And Morphology

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Effect of Ion Release on Antibacterial Activity of Melt-Derived and Sol-Gel-Derived Reactive Ceramics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.349 ◽

2006 ◽

Vol 309-311 ◽

pp. 349-354 ◽

Cited By ~ 7

Author(s):

Minna Vaahtio ◽

Eveliina Munukka ◽

Outi Leppäranta ◽

Di Zhang ◽

Erkki Eerola ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Bacterial Adhesion ◽

Antibacterial Effect ◽

Sol Gel ◽

Antimicrobial Effect ◽

Melting Process ◽

Ion Release ◽

Ph Values ◽

Ca Ions ◽

Strong Antibacterial Effect

Four different bioreactive ceramics were prepared using a sol-gel method and a standard melting process. The aim was to study antibacterial effect of these materials on the growth of Staphylococcus epidermidis. The dissolution (Si and Ca ions) of fine (≤ 45 !m) ceramic powders in a tryptone soya broth (TSB) was investigated. Also the pH changes in TSB solution were measured. In addition the adherence of Enterococcus faecalis (Ef) to materials was investigated. The bacterial adhesion was studied using scanning electron microscopy (SEM). In the bacterial and the adhesion test, CaPSiO2 and S53P4 had the strongest antimicrobial effect against studied bacteria. It was shown that bioreactive sol-gel derived ceramic with sufficient high Ca ion release (> 270 ppm) can reach strong antibacterial effect also at lower pH values (< 9.6).

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Biological and Corrosion Evaluation of In Situ Alloyed NiTi Fabricated through Laser Powder Bed Fusion (LPBF)

International Journal of Molecular Sciences ◽

10.3390/ijms222413209 ◽

2021 ◽

Vol 22 (24) ◽

pp. 13209

Author(s):

Agnieszka Chmielewska ◽

Anna Dobkowska ◽

Ewa Kijeńska-Gawrońska ◽

Michał Jakubczak ◽

Agnieszka Krawczyńska ◽

...

Keyword(s):

Niti Alloy ◽

Electrochemical Corrosion ◽

Biological Properties ◽

Passive Layer ◽

Powder Bed Fusion ◽

Laser Powder Bed Fusion ◽

Ion Release ◽

Powder Bed ◽

Phosphate Buffered Saline

In this work, NiTi alloy parts were fabricated using laser powder bed fusion (LBPF) from pre-alloyed NiTi powder and in situ alloyed pure Ni and Ti powders. Comparative research on the corrosive and biological properties of both studied materials was performed. Electrochemical corrosion tests were carried out in phosphate buffered saline at 37 °C, and the degradation rate of the materials was described based on Ni ion release measurements. Cytotoxicity, bacterial growth, and adhesion to the surface of the fabricated coupons were evaluated using L929 cells and spherical Escherichia coli (E. coli) bacteria, respectively. The in situ alloyed NiTi parts exhibit slightly lower corrosion resistance in phosphate buffered saline solution than pre-alloyed NiTi. Moreover, the passive layer formed on in situ alloyed NiTi is weaker than the one formed on the NiTi fabricated from pre-alloyed NiTi powder. Furthermore, in situ alloyed NiTi and NiTi made from pre-alloyed powders have comparable cytotoxicity and biological properties. Overall, the research has shown that nitinol sintered using in situ alloyed pure Ni and Ti is potentially useful for biomedical applications.

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Antibacterial Properties of Aloe vera on Intracanal Medicaments against Enterococcus faecalis Biofilm at Different Stages of Development

International Journal of Dentistry ◽

10.1155/2020/8855277 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Negin Ghasemi ◽

Mahsa Behnezhad ◽

Mohammad Asgharzadeh ◽

Elham Zeinalzadeh ◽

Hossein Samadi Kafil

Keyword(s):

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Antibacterial Effect ◽

Aloe Vera ◽

Antibacterial Properties ◽

Study Groups ◽

Single Root ◽

Significant Difference ◽

Planktonic Form ◽

Phosphate Buffered Saline

Background. Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the Aloe vera compound whose antibacterial effect has already been proven in the planktonic form of Enterococcus faecalis. The purpose of this study is to evaluate the antibacterial effect of Aloe vera on E. faecalis biofilms at the 4th and 6th week of development. Materials and Methods. 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with Aloe vera, calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining E. faecalis counts. Results. The CFU mean in the 4th week subgroup in Aloe vera, phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6th week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials ( p < 0.05 ). Conclusion. Aloe vera, in contrast to calcium hydroxide, eliminated 4th and 6th week biofilms and showed remarkable antibacterial properties against E. faecalis biofilm. These results support potency of Aloe vera to use as a natural antimicrobial material in the intracanal medicament.

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