Testing and performance of high-speed high-sensitivity direct-detection receivers

1992 ◽  
Author(s):  
David R. Patterson
Keyword(s):  
High Speed ◽  
Direct Detection ◽  
High Sensitivity ◽  
And Performance

scholarly journals High-Speed Interferometric Detection of Label-Free Immunoassays on the Biological Compact Disc

2006 ◽  
Vol 52 (11) ◽  
pp. 2135-2140 ◽  
Author(s):  
Ming Zhao ◽  
David Nolte ◽  
Wonryeon Cho ◽  
Fred Regnier ◽  
Manoj Varma ◽  
...  
Keyword(s):  
High Speed ◽  
Direct Detection ◽  
High Sensitivity ◽  
Compact Disc ◽  
Periodic Pattern ◽  
Label Free ◽  
Protein Patterns ◽  
Igg Antibody ◽  
Local Reference ◽  
Interferometric Detection

Abstract Background: We describe a direct-detection immunoassay that uses high-speed optical interferometry on a biological compact disc (BioCD). Methods: We fabricated phase-contrast BioCDs from 100-mm diameter 1.1-mm thick borosilicate glass disks coated with a 10-layer dielectric stack of Ta2O5/SiO2 that serves as a mirror with a center wavelength at 635 nm. The final layer is a λ/4 layer of SiO2 onto which protein patterns are immobilized through several different chemical approaches. Protein on the disc is scanned by a focused laser spot as the disc spins. Interaction of the light with the protein provides both a phase-modulated signal and a local reference that are combined interferometrically to convert phase into intensity. A periodic pattern of protein on the spinning disc produces an intensity modulation as a function of time that is proportional to the surface-bound mass. The binding of antigen or antibodies is detected directly, without labels, by a change in the interferometric intensity. The technique is demonstrated with a reverse assay of immobilized rabbit and mouse IgG antigen incubated against anti-IgG antibody in a casein buffer. Results: The signal increased with increased concentration of analyte. The current embodiment detected a concentration of 100 ng/L when averaged over ∼3000 100-micron-diameter protein spots. Conclusions: High-speed interferometric detection of label-free protein assays on a rapidly spinning BioCD is a high-sensitivity approach that is amenable to scaling up to many analytes.

scholarly journals DNA probes and PCR for diagnosis of parasitic infections.

1995 ◽  
Vol 8 (1) ◽  
pp. 113-130 ◽  
Author(s):  
J B Weiss
Keyword(s):  
Direct Detection ◽  
High Sensitivity ◽  
Clinical History ◽  
Dna Probe ◽  
Parasitic Infections ◽  
Diagnostic Assays ◽  
Reporter Probe ◽  
History Of ◽  
And Performance

DNA probe and PCR-based assays to identify and detect parasites are technically complex; however, they have high sensitivity, directly detect parasites independent of the immunocompetence or previous clinical history of the patient, and can distinguish between organisms that are morphologically similar. Diagnosis of parasites is often based on direct detection by microscopy, which is insensitive and laborious and can lack specificity. Most PCR-based assays were more sensitive than DNA probe assays. The development of PCR-based diagnostic assays requires multiple steps following the initial selection of oligonucleotide primers and reporter probe. Generally, the ability to detect the DNA of one parasite was attained by PCR; however, advances in the preparation of samples for PCR (extraction of DNA while removing PCR inhibitors) will be required to achieve that sensitivity with human specimens. Preliminary PCR systems have been developed for many different parasites, yet few have been evaluated with a large number of clinical specimens and/or under field conditions. Those evaluations are essential for determination of clinical and field utility and performance and of the most appropriate application of the assay. Several situations in which PCR-based diagnosis will result in epidemiologic, medical, or public health advances have been identified.

Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry

2019 ◽  
Author(s):  
Zachary VanAernum ◽  
Florian Busch ◽  
Benjamin J. Jones ◽  
Mengxuan Jia ◽  
Zibo Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Speed ◽  
Structural Information ◽  
Protein Complexes ◽  
High Sensitivity ◽  
Native Mass Spectrometry ◽  
Structural Features ◽  
Consumer Products ◽  
Cell Lysates ◽  
Protein Expression And Purification

It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer products, chemical processes, and therapeutics. Native mass spectrometry (nMS) has become an important tool in protein analysis due to its ability to retain non-covalent interactions during measurements, making it possible to obtain protein structural information with high sensitivity and at high speed. Interferences from the presence of non-volatiles are typically alleviated by offline buffer exchange, which is timeconsuming and difficult to automate. We provide a protocol for rapid online buffer exchange (OBE) nMS to directly screen structural features of pre-purified proteins, protein complexes, or clarified cell lysates. Information obtained by OBE nMS can be used for fast (<5 min) quality control and can further guide protein expression and purification optimization.

scholarly journals The impact of inelastic self-interacting dark matter on the dark matter structure of a Milky Way halo

2020 ◽  
Author(s):  
Kun Ting Eddie Chua ◽  
Karia Dibert ◽  
Mark Vogelsberger ◽  
Jesús Zavala
Keyword(s):  
Dark Matter ◽  
High Speed ◽  
Cold Dark Matter ◽  
Milky Way ◽  
Direct Detection ◽  
Major Axis ◽  
Inelastic Collisions ◽  
Axis Ratio ◽  
Lower Velocity ◽  
The Impact

Abstract We study the effects of inelastic dark matter self-interactions on the internal structure of a simulated Milky Way (MW)-size halo. Self-interacting dark matter (SIDM) is an alternative to collisionless cold dark matter (CDM) which offers a unique solution to the problems encountered with CDM on sub-galactic scales. Although previous SIDM simulations have mainly considered elastic collisions, theoretical considerations motivate the existence of multi-state dark matter where transitions from the excited to the ground state are exothermic. In this work, we consider a self-interacting, two-state dark matter model with inelastic collisions, implemented in the Arepo code. We find that energy injection from inelastic self-interactions reduces the central density of the MW halo in a shorter timescale relative to the elastic scale, resulting in a larger core size. Inelastic collisions also isotropize the orbits, resulting in an overall lower velocity anisotropy for the inelastic MW halo. In the inner halo, the inelastic SIDM case (minor-to-major axis ratio s ≡ c/a ≈ 0.65) is more spherical than the CDM (s ≈ 0.4), but less spherical than the elastic SIDM case (s ≈ 0.75). The speed distribution f(v) of dark matter particles at the location of the Sun in the inelastic SIDM model shows a significant departure from the CDM model, with f(v) falling more steeply at high speeds. In addition, the velocity kicks imparted during inelastic collisions produce unbound high-speed particles with velocities up to 500 km s−1 throughout the halo. This implies that inelastic SIDM can potentially leave distinct signatures in direct detection experiments, relative to elastic SIDM and CDM.

scholarly journals Spatially Resolved Experimental Modal Analysis on High-Speed Composite Rotors Using a Non-Contact, Non-Rotating Sensor

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4705
Author(s):  
Julian Lich ◽  
Tino Wollmann ◽  
Angelos Filippatos ◽  
Maik Gude ◽  
Juergen Czarske ◽  
...  
Keyword(s):  
High Speed ◽  
Natural Frequencies ◽  
Mode Shapes ◽  
Fiber Reinforced ◽  
Structural Dynamic ◽  
Spatially Resolved ◽  
Glass Fiber Reinforced ◽  
Vibration Responses ◽  
And Performance

Due to their lightweight properties, fiber-reinforced composites are well suited for large and fast rotating structures, such as fan blades in turbomachines. To investigate rotor safety and performance, in situ measurements of the structural dynamic behaviour must be performed during rotating conditions. An approach to measuring spatially resolved vibration responses of a rotating structure with a non-contact, non-rotating sensor is investigated here. The resulting spectra can be assigned to specific locations on the structure and have similar properties to the spectra measured with co-rotating sensors, such as strain gauges. The sampling frequency is increased by performing consecutive measurements with a constant excitation function and varying time delays. The method allows for a paradigm shift to unambiguous identification of natural frequencies and mode shapes with arbitrary rotor shapes and excitation functions without the need for co-rotating sensors. Deflection measurements on a glass fiber-reinforced polymer disk were performed with a diffraction grating-based sensor system at 40 measurement points with an uncertainty below 15 μrad and a commercial triangulation sensor at 200 measurement points at surface speeds up to 300 m/s. A rotation-induced increase of two natural frequencies was measured, and their mode shapes were derived at the corresponding rotational speeds. A strain gauge was used for validation.

40 Gb/s High-speed mode-division multiplexing transmission employing NRZ modulation format

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Rabiu Imam Sabitu ◽  
Nafizah Goriman Khan ◽  
Amin Malekmohammadi
Keyword(s):  
System Performance ◽  
High Speed ◽  
Direct Detection ◽  
Signal To Noise Ratio ◽  
Optical Signal ◽  
Threshold Power ◽  
Modulation Format ◽  
Transmission Distance ◽  
Mode Division Multiplexing ◽  
Speed Mode

AbstractThis report examines the performance of a high-speed MDM transmission system supporting four nondegenerate spatial modes at 10 Gb/s. The analysis adopts the NRZ modulation format to evaluate the system performance in terms of a minimum power required (PN) and the nonlinear threshold power (PTH) at a BER of 10−9. The receiver sensitivity, optical signal-to-noise ratio, and the maximum transmission distance were investigated using the direct detection by employing a multimode erbium-doped amplifier (MM-EDFA). It was found that by properly optimizing the MM-EDFA, the system performance can significantly be improved.

scholarly journals Biological nanoscale fluorescent probes: From structure and performance to bioimaging

2020 ◽  
Vol 39 (1) ◽  
pp. 209-221
Author(s):  
Jiafeng Wan ◽  
Xiaoyuan Zhang ◽  
Kai Zhang ◽  
Zhiqiang Su
Keyword(s):  
Fluorescent Probes ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Biological Imaging ◽  
Fluorescent Materials ◽  
And Performance

Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.

Image-Based Versus Signal-Based Sensors for Machine Fault Detection and Isolation

2014 ◽  
Author(s):  
Heshan Fernando ◽  
Vedang Chauhan ◽  
Brian Surgenor
Keyword(s):  
Fault Detection ◽  
High Speed ◽  
Fault Isolation ◽  
Video Data ◽  
Fault Detection And Isolation ◽  
Single Camera ◽  
Multiple Faults ◽  
Multiple Sensors ◽  
Machine Fault ◽  
And Performance

This paper presents the results of a comparative study that investigated the use of image-based and signal-based sensors for fault detection and fault isolation of visually-cued faults on an automated assembly machine. The machine assembles 8 mm circular parts, from a bulk-supply, onto continuously moving carriers at a rate of over 100 assemblies per minute. Common faults on the machine include part jams and ejected parts that occur at different locations on the machine. Two sensor systems are installed on the machine for detecting and isolating these faults: an image-based system consisting of a single camera and a signal-based sensor system consisting of multiple greyscale sensors and limit switches. The requirements and performance of both systems are compared for detecting six faults on the assembly machine. It is found that both methods are able to effectively detect the faults but they differ greatly in terms of cost, ease of implementation, detection time and fault isolation capability. The conventional signal-based sensors are low in cost, simple to implement and require little computing power, but the installation is intrusive to the machine and readings from multiple sensors are required for faster fault detection and isolation. The more sophisticated image-based system requires an expensive, high-resolution, high-speed camera and significantly more processing power to detect the same faults; however, the system is not intrusive to the machine, fault isolation becomes a simpler problem with video data, and the single camera is able to detect multiple faults in its field of view.

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