scholarly journals Coexistence of Two bla NDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Yu Feng ◽  
Lu Liu ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Nanopore Sequencing ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Transmissible Plasmid

ABSTRACT In a carbapenem-resistant Escherichia coli clinical isolate of sequence type 167, two copies of bla NDM-5 were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B − type. Both bla NDM-5 genes were located in 11,065-bp regions flanked by two copies of IS 26 . The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of bla NDM-5 .

scholarly journals Sequence Type 273 Carbapenem-Resistant Klebsiella pneumoniae Carrying bla NDM-1 and bla IMP-4

2018 ◽  
Vol 62 (6) ◽  
Author(s):  
Lu Liu ◽  
Yu Feng ◽  
Haiyan Long ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Klebsiella Pneumoniae ◽  
Southeast Asia ◽  
Human Blood ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Transmissible Plasmid ◽  
Long Read

ABSTRACT A carbapenem-resistant Klebsiella pneumoniae isolate was recovered from human blood. Its whole-genome sequence was obtained using Illumina and long-read MinION sequencing. The strain belongs to sequence type 273 (ST273), which was found recently and caused an outbreak in Southeast Asia. It has two carbapenemase genes, bla NDM-1 (carried by an ST7 IncN self-transmissible plasmid) and bla IMP-4 (located on a self-transmissible IncHI5 plasmid). Non-KPC-producing ST237 may represent a lineage of carbapenem-resistant K. pneumoniae , which warrants further monitoring.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

scholarly journals Draft Genome Sequence of an Escherichia coli Sequence Type 410 Isolate Harboring blaNDM-5 Genes with gyrA and parE Mutations, Isolated from Urine Culture in China

2019 ◽  
Vol 8 (50) ◽  
Author(s):  
Junwei Huang ◽  
Shanshan Ma ◽  
Qian Yu ◽  
Miao Fu ◽  
Lingli Shao
Keyword(s):  
Escherichia Coli ◽  
High Risk ◽  
Clinical Isolate ◽  
Genome Sequence ◽  
Urine Culture ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Content Type

Escherichia coli sequence type 410 (ST410) is now an international high-risk clone and is responsible for a large number of clinical infections. Here, we report the draft genome sequence of an ST410 clinical isolate harboring multiple bla NDM-5 genes that was obtained from urine culture in China.

scholarly journals A Novel Deletion Mutation in pmrB Contributes to Concurrent Colistin Resistance in Carbapenem-Resistant Escherichia coli Sequence Type 405 of Clinical Origin

2020 ◽  
Vol 64 (6) ◽  
Author(s):  
Ching-Hsun Wang ◽  
L. Kristopher Siu ◽  
Feng-Yee Chang ◽  
Yu-Kuo Tsai ◽  
Yi-Tsung Lin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Dna ◽  
Selective Pressure ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Wild Type ◽  
Colistin Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Recombinant Dna Techniques

ABSTRACT We report the first clinical Escherichia coli strain EC3000 with concomitant chromosomal colistin and carbapenem resistance. A novel in-frame deletion, Δ6-11 (RPISLR), in pmrB that contributes to colistin resistance was verified using recombinant DNA techniques. Although being less fit than the wild-type (WT) strain or EC3000 revertant (chromosomal replacement of WT pmrB in EC3000), a portion of serially passaged EC3000 strains preserving colistin resistance without selective pressure raises the concern for further spread.

scholarly journals New Variant of mcr-3 in an Extensively Drug-Resistant Escherichia coli Clinical Isolate Carrying mcr-1 and blaNDM-5

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Lu Liu ◽  
Yu Feng ◽  
Xiaoxia Zhang ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Amino Acid Substitutions ◽  
Drug Resistant ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Resistant Genes ◽  
Extensively Drug Resistant ◽  
New Variant ◽  
Carbapenemase Gene

ABSTRACT A colistin- and carbapenem-resistant Escherichia coli clinical isolate was found to carry two plasmid-borne colistin-resistant genes, mcr-1 and the newly identified mcr-3, and a carbapenemase gene, bla NDM-5. mcr-3 is a new variant (mcr-3.5) in the isolate and encodes three amino acid substitutions compared with the original MCR-3. mcr-3 was carried by a TnAs3-like transposon on a self-transmissible IncP plasmid in the isolate, highlighting that mcr-3 may have widely spread.

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

scholarly journals Identification of a Novel NDM Variant, NDM-13, from a Multidrug-Resistant Escherichia coli Clinical Isolate in Nepal

2015 ◽  
Vol 59 (9) ◽  
pp. 5847-5850 ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Kayo Shimada ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enzymatic Activity ◽  
Clinical Isolate ◽  
Multidrug Resistant ◽  
New Delhi ◽  
Content Type ◽  
Genetic Environment ◽  
Carbapenem Resistant

ABSTRACTA novel New Delhi metallo-β-lactamase, NDM-13, was identified in a carbapenem-resistantEscherichia coliclinical isolate obtained from the urine of a patient in Nepal. The enzymatic activity of NDM-13 against β-lactams was similar to that of NDM-1. However, NDM-13 displayed significantly higherkcat/Kmratios for cefotaxime. The genetic environment ofblaNDM-13was determined to betnpA-IS30-blaNDM-13-bleMBL-trpF-dsbC-cutA-groES-groL, withblaNDM-13located within the chromosome.

scholarly journals Molecular Characterization ofblaNDM-5Carried on an IncFII Plasmid in an Escherichia coli Isolate from a Nontraveler Patient in Spain

2014 ◽  
Vol 59 (1) ◽  
pp. 659-662 ◽  
Author(s):  
Cristina Pitart ◽  
Mar Solé ◽  
Ignasi Roca ◽  
Angely Román ◽  
Asunción Moreno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Female Patient ◽  
Urine Culture ◽  
Sequence Type ◽  
Content Type ◽  
Pcr Screening ◽  
Coli Isolate ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Indian Origin

ABSTRACTA carbapenem-resistantEscherichia coliisolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence ofblaNDM-5,blaTEM-1,blaOXA-1,blaCMY-42, andrmtB. blaNDM-5was carried in a conjugative IncFII-type plasmid (90 kb) together withblaTEM-1andrmtB. The genetic environment ofblaNDM-5showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France inE. coliof African and Indian origin, respectively.

scholarly journals Widespread Dissemination of Carbapenem-Resistant Escherichia coli Sequence Type 167 Strains HarboringblaNDM-5in Clinical Settings in China

2016 ◽  
Vol 60 (7) ◽  
pp. 4364-4368 ◽  
Author(s):  
Yonglu Huang ◽  
Xiaonan Yu ◽  
Miaomiao Xie ◽  
Xuan Wang ◽  
Kang Liao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Clinical Settings ◽  
Content Type ◽  
Carbapenem Resistant ◽  
The Future ◽  
Widespread Dissemination

ABSTRACTThis study reports the increasing prevalence of clinicalEscherichia coliof sequence type 167 (ST167) carrying bothblaNDM-1andblaNDM-5on the conjugative IncX3 plasmid in various parts of China. Close surveillance is needed to monitor the future dissemination of ST167 strains that harborblaNDM-5or otherblaNDM-like genes.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

Sign in / Sign up

Export Citation Format

Share Document