scholarly journals Widespread Dissemination of Carbapenem-Resistant Escherichia coli Sequence Type 167 Strains HarboringblaNDM-5in Clinical Settings in China

2016 ◽  
Vol 60 (7) ◽  
pp. 4364-4368 ◽  
Author(s):  
Yonglu Huang ◽  
Xiaonan Yu ◽  
Miaomiao Xie ◽  
Xuan Wang ◽  
Kang Liao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Clinical Settings ◽  
Content Type ◽  
Carbapenem Resistant ◽  
The Future ◽  
Widespread Dissemination

ABSTRACTThis study reports the increasing prevalence of clinicalEscherichia coliof sequence type 167 (ST167) carrying bothblaNDM-1andblaNDM-5on the conjugative IncX3 plasmid in various parts of China. Close surveillance is needed to monitor the future dissemination of ST167 strains that harborblaNDM-5or otherblaNDM-like genes.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

scholarly journals A Novel Deletion Mutation in pmrB Contributes to Concurrent Colistin Resistance in Carbapenem-Resistant Escherichia coli Sequence Type 405 of Clinical Origin

2020 ◽  
Vol 64 (6) ◽  
Author(s):  
Ching-Hsun Wang ◽  
L. Kristopher Siu ◽  
Feng-Yee Chang ◽  
Yu-Kuo Tsai ◽  
Yi-Tsung Lin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Dna ◽  
Selective Pressure ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Wild Type ◽  
Colistin Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Recombinant Dna Techniques

ABSTRACT We report the first clinical Escherichia coli strain EC3000 with concomitant chromosomal colistin and carbapenem resistance. A novel in-frame deletion, Δ6-11 (RPISLR), in pmrB that contributes to colistin resistance was verified using recombinant DNA techniques. Although being less fit than the wild-type (WT) strain or EC3000 revertant (chromosomal replacement of WT pmrB in EC3000), a portion of serially passaged EC3000 strains preserving colistin resistance without selective pressure raises the concern for further spread.

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

scholarly journals Molecular Characterization ofblaNDM-5Carried on an IncFII Plasmid in an Escherichia coli Isolate from a Nontraveler Patient in Spain

2014 ◽  
Vol 59 (1) ◽  
pp. 659-662 ◽  
Author(s):  
Cristina Pitart ◽  
Mar Solé ◽  
Ignasi Roca ◽  
Angely Román ◽  
Asunción Moreno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Female Patient ◽  
Urine Culture ◽  
Sequence Type ◽  
Content Type ◽  
Pcr Screening ◽  
Coli Isolate ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Indian Origin

ABSTRACTA carbapenem-resistantEscherichia coliisolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence ofblaNDM-5,blaTEM-1,blaOXA-1,blaCMY-42, andrmtB. blaNDM-5was carried in a conjugative IncFII-type plasmid (90 kb) together withblaTEM-1andrmtB. The genetic environment ofblaNDM-5showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France inE. coliof African and Indian origin, respectively.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals Coexistence of Two bla NDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Yu Feng ◽  
Lu Liu ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Nanopore Sequencing ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Transmissible Plasmid

ABSTRACT In a carbapenem-resistant Escherichia coli clinical isolate of sequence type 167, two copies of bla NDM-5 were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B − type. Both bla NDM-5 genes were located in 11,065-bp regions flanked by two copies of IS 26 . The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of bla NDM-5 .

scholarly journals Widespread Dissemination of CTX-M-15 Genotype Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae among Patients Presenting to Community Hospitals in the Southeastern United States

2013 ◽  
Vol 58 (2) ◽  
pp. 1200-1202 ◽  
Author(s):  
Luke F. Chen ◽  
Joshua T. Freeman ◽  
Brad Nicholson ◽  
Anna Keiger ◽  
Sarah Lancaster ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Southeastern United States ◽  
Sequence Type ◽  
Community Hospitals ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Widespread Dissemination ◽  
M 14

ABSTRACTExtended-spectrum-β-lactamase (ESBL)-producing organisms are increasingly prevalent. We determined the characteristics of 66 consecutive ESBL-producing isolates from six community hospitals in North Carolina and Virginia from 2010 to 2012. Fifty-three (80%) ESBL-producing isolates contained CTX-M enzymes; CTX-M-15 was found in 68% ofEscherichia coliand 73% ofKlebsiellaisolates. Sequence type 131 (ST131) was the commonest type ofE. coli, accounting for 48% of CTX-M-15-producing and 66% of CTX-M-14-producing isolates. In conclusion, the CTX-M genotype and ST131E. coliwere common among ESBL isolates from U.S. community hospitals.

scholarly journals Successful Treatment of Severe Post-craniotomy Meningitis Caused by an Escherichia coli Sequence Type 410 Strain Coharboring blaNDM–5 and blaCTX–M–65

2021 ◽  
Vol 12 ◽  
Author(s):  
Qing Yang ◽  
Piao-piao Zhang ◽  
Yan Jiang ◽  
Xiu-jue Zheng ◽  
Min Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Successful Treatment ◽  
Treatment Options ◽  
Treatment Protocol ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Control Measures ◽  
Clinical Settings ◽  
Infection Control Measures ◽  
Carbapenem Resistant

Intracranial infections caused by multidrug-resistant Gram-negative bacterium have led to considerable mortality due to extremely limited treatment options. Herein, we firstly reported a clinical carbapenem-resistant Escherichia coli isolate coharboring blaNDM–5 and blaCTX–M–65 from a patient with post-craniotomy meningitis. The carbapenem-resistant Escherichia coli strain CNEC001 belonging to Sequence Type 410 was only susceptible to amikacin and tigecycline, both of which have poor penetration through the blood-brain barrier (BBB). The blaCTX–M–65 gene was expressed on a 135,794 bp IncY plasmid. The blaNDM–5 gene was located on a genomic island region of an IncX3-type plasmid pNDM5-CNEC001. Based on the characteristics of the strain, we presented the successful treatment protocol of intravenous (IV) tigecycline and amikacin combined with intrathecal (ITH) amikacin in this study. Intracranial infection caused by Escherichia coli coharboring blaNDM–5 and blaCTX–M–65 is rare and fatal. Continuous surveillance and infection control measures for such strain need critical attention in clinical settings.

scholarly journals Plasmid-Mediated Novel blaNDM-17 Gene Encoding a Carbapenemase with Enhanced Activity in a Sequence Type 48 Escherichia coli Strain

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Zhihai Liu ◽  
Yang Wang ◽  
Timothy R. Walsh ◽  
Dejun Liu ◽  
Zhangqi Shen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Treatment Options ◽  
Coli Strain ◽  
Sequence Type ◽  
Animal Origin ◽  
New Delhi ◽  
Gene Encoding ◽  
Content Type ◽  
Carbapenem Resistant

ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) have spread worldwide, leaving very few treatment options available. New Delhi metallo-beta-lactamase (NDM) is the main carbapenemase mediating CRE resistance and is of increasing concern. NDM-positive Enterobacteriaceae of human origin are frequently identified; however, the emergence of NDM, and particularly novel variants, in bacteria of food animal origin has never been reported. Here, we characterize a novel NDM variant (assigned NDM-17) identified in a β-lactam-resistant sequence type 48 (ST48) Escherichia coli strain that was isolated from a chicken in China. Compared to NDM-1, NDM-17 had three amino acid substitutions (V88L, M154L, and E170K) that confer significantly enhanced carbapenemase activity. Compared to NDM-5, NDM-17 had only one amino acid substitution (E170K) and slightly increased isolate resistance to carbapenem, as indicated by increased MIC values. The gene encoding NDM-17 (bla NDM-17) was located on an IncX3 plasmid, which was readily transferrable to recipient E. coli strain J53 by conjugation, suggesting the possibility of the rapid dissemination of bla NDM-17. Enzyme kinetics showed that NDM-17 could hydrolyze all β-lactams tested, except for aztreonam, and had a significantly higher affinity for all β-lactams tested than did NDM-5. The emergence of this novel NDM variant could pose a threat to public health because of its transferability and enhanced carbapenemase activity.

scholarly journals Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae Mediated by Chromosomal Integration of Plasmid DNA

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Astrid V. Cienfuegos-Gallet ◽  
Liang Chen ◽  
Barry N. Kreiswirth ◽  
J. Natalia Jiménez
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plasmid Dna ◽  
Clonal Expansion ◽  
Genetic Alterations ◽  
Sequence Type ◽  
Chromosomal Integration ◽  
Colistin Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Single Locus Variant

ABSTRACT Here we describe the spread of colistin resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae in Medellín, Colombia. Among 32 isolates collected between 2012 and 2014, 24 showed genetic alterations in mgrB. Nineteen isolates belonged to sequence type 512 (ST512) (or its single locus variant [SLV]) and harbored an 8.1-kb hsdMSR insertion corresponding to ISKpn25, indicating a clonal expansion of the resistant strain. The insertion region showed 100% identity to several plasmids, suggesting that the colistin resistance is mediated by chromosomal integration of plasmid DNA.

Sign in / Sign up

Export Citation Format

Share Document