scholarly journals Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany

2021 ◽  
Vol 9 (7) ◽  
pp. 1345
Author(s):  
Stefan E. Heiden ◽  
Katharina Sydow ◽  
Stephan Schaefer ◽  
Ingo Klempien ◽  
Veronika Balau ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Bacterial Species ◽  
Genomic Analysis ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
Mercury Resistance ◽  
Major Public Health Problem ◽  
Eastern Germany ◽  
North Eastern ◽  
Resistance Plasmids

The emergence of carbapenemase-producing Enterobacteriaceae limits therapeutic options and presents a major public health problem. Resistances to carbapenems are mostly conveyed by metallo-beta-lactamases (MBL) including VIM, which are often encoded on resistance plasmids. We characterized four VIM-positive isolates that were obtained as part of a routine diagnostic screening from two laboratories in north-eastern Germany between June and August 2020. Whole-genome sequencing was performed to address (a) phylogenetic properties, (b) plasmid content, and (c) resistance gene carriage. In addition, we performed phenotypic antibiotic and mercury resistance analyses. The genomic analysis revealed three different bacterial species including C. freundii, E. coli and K. oxytoca with four different sequence types. All isolates were geno- and phenotypically multidrug-resistant (MDR) and the phenotypic profile was explained by the underlying resistance gene content. Three isolates of four carried nearly identical VIM-1-resistance plasmids, which in addition encoded a mercury resistance operon and showed some similarity to two publicly available plasmid sequences from sources other than the two laboratories above. Our results highlight the circulation of a nearly identical IncN-type VIM-1-resistance plasmid in different Enterobacteriaceae in north-eastern Germany.

scholarly journals Multidrug-resistant typhoid fever: a review

2011 ◽  
Vol 5 (05) ◽  
pp. 324-337 ◽  
Author(s):  
Syed Ahmed Zaki ◽  
Sunil Karande
Keyword(s):  
Public Health ◽  
Typhoid Fever ◽  
English Language ◽  
Public Health Problem ◽  
Public Health Education ◽  
Abdominal Distension ◽  
Clinical Results ◽  
Multidrug Resistant ◽  
Major Public Health Problem ◽  
Handling Practices

Introduction: Multidrug-resistant typhoid fever (MDRTF) is defined as typhoid fever caused by Salmonella enterica serovar Typhi strains (S. Typhi), which are resistant to the first-line recommended drugs for treatment such as chloramphenicol, ampicillin and trimethoprim-sulfamethoxazole. Since the mid-1980s, MDRTF has caused outbreaks in several countries in the developing world, resulting in increased morbidity and mortality, especially in affected children below five years of age and those who are malnourished. Methodology: Two methods were used to gather the information presented in this article. First PubMed was searched for English language references to published relevant articles. Secondly, chapters on typhoid fever in standard textbooks of paediatric infectious diseases and preventive and social medicine were reviewed. Results: Although there are no pathognomonic clinical features of MDRTF at the onset of the illness, high fever ( > 104°F), toxaemia, abdominal distension, abdominal tenderness, hepatomegaly and splenomegaly are often reported. The gold standard for the diagnosis of MDRTF is bacterial isolation of the organism in blood cultures. Ciprofloxacin and ceftriaxone are the drugs most commonly used for treatment of MDRTF and produce good clinical results. Conclusion: MDRTF remains a major public health problem, particularly in developing countries. Mass immunization in endemic areas with either the oral live attenuated Typhi 21a or the injectable unconjugated Vi typhoid vaccine, rational use of antibiotics, improvement in public sanitation facilities, availability of clean drinking water, promotion of safe food handling practices and public health education are vital in the prevention of MDRTF. 

scholarly journals Trends and Risk Factors for Antimicrobial-Resistant Neisseria gonorrhoeae, Melbourne, Australia, 2007 to 2018

2019 ◽  
Vol 63 (10) ◽  
Author(s):  
Deborah A. Williamson ◽  
Christopher K. Fairley ◽  
Benjamin P. Howden ◽  
Marcus Y. Chen ◽  
Kerrie Stevens ◽  
...  
Keyword(s):  
Risk Factors ◽  
Neisseria Gonorrhoeae ◽  
Laboratory Data ◽  
Northeast Asia ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
International Travel ◽  
Major Public Health Problem ◽  
Comprehensive Overview ◽  
Content Type

ABSTRACT Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a major public health problem. Traditionally, AMR surveillance programs for N. gonorrhoeae have focused mainly on laboratory data to describe the prevalence and trends of resistance. However, integrating individual-level risk factors (e.g., sexual orientation or international travel) with laboratory data provides important insights into factors promoting the spread of resistant N. gonorrhoeae. Here, over a 12-year period, we assessed the trends and risk factors for resistant N. gonorrhoeae in individuals attending a large publicly funded sexual health center in Melbourne, Australia. A total of 7,588 N. gonorrhoeae isolates were cultured from 5,593 individuals between 1 January 2007 and 31 December 2018. The proportion of isolates with penicillin resistance decreased from 49.5% in 2007 to 18.3% in 2018 (ptrend < 0.001) and from 63.5% in 2007 to 21.1% in 2018 for ciprofloxacin resistance (ptrend < 0.001). In contrast, the proportion of isolates displaying decreased susceptibility to ceftriaxone increased from 0.5% in 2007 to 2.9% in 2018 (ptrend < 0.001), with a significant increase in low-level azithromycin resistance, from 2.5% in 2012 to 8.2% in 2018 (ptrend < 0.001). Multivariate analysis identified risk factors for multidrug-resistant (MDR) N. gonorrhoeae, namely, female sex and country of birth, with MDR isolates more common in individuals born in northeast Asia, further highlighting the importance of this region and international travel as factors in the cross-border transmission of MDR N. gonorrhoeae. Future surveillance work should incorporate additional epidemiological and genomic data to provide a comprehensive overview of the emergence and spread of resistant N. gonorrhoeae.

scholarly journals Genomic analysis and morphological characterization of bacteriophages for Salmonella spp. treatment in poultry

2018 ◽  
Author(s):  
Aline Parolin Calarga ◽  
Marcelo Brocchi
Keyword(s):  
Therapeutic Potential ◽  
Genomic Analysis ◽  
Storage Period ◽  
Public Health Problem ◽  
Morphological Characterization ◽  
Major Public Health Problem ◽  
Salmonella Spp ◽  
C Elegans

Enteric infections caused by Salmonella spp. represent a major public health problem worldwide, due to the large proportion of foodborne infections derived from this pathogen. Currently, antimicrobials are used to prevent contamination of chicken meat. However, in order to combat salmonellosis without the propagation of resistant strains, it is necessary to study alternative therapeutic approaches, such as the use of bacteriophages against Salmonellosis. For the present work bacteriophages provided by FMRP-USP were selected to further studies on its therapeutic potential. In addition, we work with lytic bacteriophages induced from monophasic strains of Salmonella spp. Our initial aim for this project was the morphological and molecular characterization of these viruses. Nevertheless, the environmental phages did not survive the storage period. Due to these results, our further studies will be focused on the lytic phages. They will be tested in vivo in the C. elegans model in order to evaluate the survival rate of the worms when infected with Salmonella spp.

scholarly journals Epidemiological Profile and Antimicrobial Resistance Pattern of Enteric Fever in a Tertiary Care Hospital of North India – a Seven Year Ambispective Study

2018 ◽  
Vol 61 (4) ◽  
pp. 125-130 ◽  
Author(s):  
Anuradha Makkar ◽  
Shilpi Gupta ◽  
Inam Danish Khan ◽  
Rajiv Mohan Gupta ◽  
KS Rajmohan ◽  
...  
Keyword(s):  
Public Health ◽  
Enteric Fever ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
North India ◽  
Major Public Health Problem ◽  
Care Hospital ◽  
Drug Resistant

Introduction: Enteric-fever is a major public-health problem in developing countries emerging as multidrug-resistant, Nalidixic-acid resistant and extremely drug-resistant Salmonella (Pakistan, 2016), has intensified the use of WHO watch/reserve group antimicrobials such as azithromycin and meropenem. Methods: This ambispective-study was conducted on 782 non-repeat blood-culture isolates of S. Typhi, S. Paratyphi A and S. Paratyphi B obtained from 29,184 blood cultures received at a 1000-bedded tertiary-care hospital of North-India from 2011–2017. Identification and antibiograms were obtained by Vitek-2 compact and Kirby-Bauer’s disc diffusion with resistance to ampicillin, chloramphenicol and cotrimoxazole being labeled as multidrug-resistant. Decreased ciprofloxacin-susceptibility and ciprofloxacin-resistance were defined as MIC 0.125–0.5 and >1 μg/ml. Results: S. Typhi and S. Paratyphi A in a ratio of 3.9:1 were seen between July–September predominantly distributed between 6–45 year age group. Resistance to co-trimoxazole, chloramphenicol, ceftriaxone and azithromycin was 6.1%, 13.8%, 16.1 and 5.78% respectively. Multidrug-resistant S. typhi and S. paratyphi A were 2.73% and 1.91% respectively. Conclusion: Enteric-fever is a major public-health problem in India. Emergence of multidrug-resistant, Nalidixic-acid resistant and extremely-drug resistant Salmonella mandates ongoing surveillance for targeted empirical therapy and containment of spread. Repeated epidemics call for water, sanitation, hygiene and vaccination strategies to sustain herd-immunity.

Characterization of Quinolone Resistance in Salmonella enterica from Farm Animals in China

2017 ◽  
Vol 80 (10) ◽  
pp. 1742-1748 ◽  
Author(s):  
Ting-Ting Cao ◽  
Guo-Hui Deng ◽  
Liang-Xing Fang ◽  
Run-Shi Yang ◽  
Jian Sun ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Salmonella Enterica ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Quinolone Resistance ◽  
Farm Animals ◽  
Gyra Gene ◽  
Republic Of China ◽  
Resistance Plasmids

ABSTRACT This study was focused on the prevalence and antimicrobial susceptibilities of Salmonella directly isolated at animal clinics in Guangdong, People's Republic of China. The isolation rates from chickens, ducks, and pigs were 11.3% (11 of 97 samples), 15.4% (53 of 344 samples), and 3.0% (13 of 434 samples), respectively. Among the 77 Salmonella enterica isolates, the most predominant serovar was Typhimurium (81.8%, 63 isolates), followed by serovars Meleagridis (2.6%, 2 isolates) and Abaetetuba (1.3%, 1 isolate). Salmonella isolates were resistant to ciprofloxacin (16.9% of isolates) and nalidixic acid (66.2% of isolates), and 68 isolates (88.3%) were multidrug resistant, displaying resistance to three or more classes of antimicrobial agents. Eighteen isolates (23.4%) had at least one plasmid-mediated quinolone resistance gene, which was identified using PCR and DNA sequencing. The most prevalent plasmid-mediated quinolone resistance gene was aac(6′)-Ib-cr, found in 14 isolates (18.2%), followed by oqxAB (9.1%) and qnrS (7.8%). Alterations in the gyrA gene were detected in 24 (57.1%) of 42 strains with a ciprofloxacin MIC of ≥0.25 μg/mL; the same level of susceptibility was found for enrofloxacin. Six types of mutations were found in the quinolone resistance determining regions of gyrA, and the predominant one (S83Y) was found singly in 15 (62.5%) of 24 isolates. We also found 22 different pulsed-field gel electrophoresis types among the Salmonella isolates. The Salmonella serovars and MICs of ciprofloxacin were similar within clusters, although individual differences were noted. This finding suggests that resistance plasmids were horizontally transmitted but also clonally spread.

scholarly journals MODS-Wayne, a Colorimetric Adaptation of the Microscopic-Observation Drug Susceptibility (MODS) Assay for Detection ofMycobacterium tuberculosisPyrazinamide Resistance from Sputum Samples

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Roberto Alcántara ◽  
Patricia Fuentes ◽  
Ricardo Antiparra ◽  
Marco Santos ◽  
Robert H. Gilman ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Color Change ◽  
Hydrolysis Product ◽  
Public Health Problem ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Major Public Health Problem ◽  
Ferrous Ammonium Sulfate ◽  
Kappa Index ◽  
Resistant Tuberculosis

ABSTRACTAlthough pyrazinamide (PZA) is a key component of first- and second-line tuberculosis treatment regimens, there is no gold standard to determine PZA resistance. Approximately 50% of multidrug-resistant tuberculosis (MDR-TB) and over 90% of extensively drug-resistant tuberculosis (XDR-TB) strains are also PZA resistant.pncAsequencing is the endorsed test to evaluate PZA susceptibility. However, molecular methods have limitations for their wide application. In this study, we standardized and evaluated a new method, MODS-Wayne, to determine PZA resistance. MODS-Wayne is based on the detection of pyrazinoic acid, the hydrolysis product of PZA, directly in the supernatant of sputum cultures by detecting a color change following the addition of 10% ferrous ammonium sulfate. Using a PZA concentration of 800 µg/ml, sensitivity and specificity were evaluated at three different periods of incubation (reading 1, reading 2, and reading 3) using a composite reference standard (MGIT-PZA,pncAsequencing, and the classic Wayne test). MODS-Wayne was able to detect PZA resistance, with a sensitivity and specificity of 92.7% and 99.3%, respectively, at reading 3. MODS-Wayne had an agreement of 93.8% and a kappa index of 0.79 compared to the classic Wayne test, an agreement of 95.3% and kappa index of 0.86 compared to MGIT-PZA, and an agreement of 96.9% and kappa index of 0.90 compared topncAsequencing. In conclusion, MODS-Wayne is a simple, fast, accurate, and inexpensive approach to detect PZA resistance, making this an attractive assay especially for low-resource countries, where TB is a major public health problem.

scholarly journals Paediatric Multidrug-Resistant Tuberculosis with HIV Coinfection: A Case Report

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Huldah I. Nwokeukwu ◽  
Paulinus N. Okafor ◽  
Onuka Okorie ◽  
Ihuoma K. Ukpabi
Keyword(s):  
Public Health Problem ◽  
Multidrug Resistant ◽  
Active Tuberculosis ◽  
Recurrent Fever ◽  
Major Public Health Problem ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Hiv Coinfection ◽  
Mdr Tb ◽  
History Of

Background.Tuberculosis is a major public health problem, and its control has been facing a lot of challenges with emergence of HIV. The occurrence of multidrug-resistant strain has also propounded the problem especially in children where diagnosis is difficult to make. Multidrug-resistant tuberculosis (MDR-TB) isin vitroresistant to isoniazid (H) and rifampicin (R). Paediatric multi-drug resistant tuberculosis with HIV coinfection is rare, and there is no documented report from Nigeria.Objective.To report a case of paediatric MDR-TB in Nigeria about it.Methods.The case note of the patient was retrieved, and relevant data were extracted and summarized.Results.A 9-year-old female HIV-positive pupil with a year history of recurrent cough, 3 months history of recurrent fever, and generalized weight loss was diagnosed and treated for tuberculosis but failed after retreatment. She was later diagnosed with MDR-TB and is presently on DOT-Plus regimen.Conclusion.Paediatric MDR-TB with HIV co-infection is rare. Early diagnosis and treatment is important to prevent spread of the disease. The use of Isoniazid preventive therapy is recommended for children who come in contact with patients with active tuberculosis and also for HIV patients without active tuberculosis.

scholarly journals A Review of Superbug: A Global Threat in Health Care System

2018 ◽  
Vol 4 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Bhuiyan Mohammad Mahtab Uddin ◽  
Md Abdullah Yusuf ◽  
Zubair Ahmed Ratan
Keyword(s):  
Public Health ◽  
Health Problem ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Major Public Health Problem ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Hospital Acquired

The rapid spread and dissemination of the multidrug-resistant bacteria worldwide represents a major public health problem. The development of antibiotics decreased the mortality among the human and animals leading to a better life expectancy. But the injudicious use of antimicrobials and selection pressure the microbes have developed resistance which became more prominent during last few decades. With the evolution of Methicilin-resistant Staphylococcus aureus (MRSA), Hospital-acquired MRSA, Communityacquired MRSA and MDR TB (Multidrug resistant tuberculosis) challenge for the clinicians have increased to a greater extent. The global emergence and dissemination of acquired carbapenemases among gram negative bacteria are considered a major public health problem. Gram-negative bacteria, most notably Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, are among the most important causes of serious hospital-acquired and community-onset bacterial infections in humans, and resistance to antimicrobial agents in these bacteria has become an increasingly relevant problem. Recent development in nanotechnology based drug delivery system may prove to be solution for combating these resistant bacteria. However policies and regulations for antibiotic use should be formulated to control the further development of resistance among the microbes.Bangladesh Journal of Infectious Diseases 2017;4(1):25-28

scholarly journals Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia

2020 ◽  
Vol 69 (7) ◽  
pp. 1013-1019
Author(s):  
Tryna Tania ◽  
Pratiwi Sudarmono ◽  
R. Lia Kusumawati ◽  
Andriansjah Rukmana ◽  
Wahyu Agung Pratama ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Public Health Problem ◽  
Multidrug Resistant ◽  
Drug Susceptibility Testing ◽  
Major Public Health Problem ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Mdr Tb

Introduction. Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia. Aim. We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant Mycobacterium tuberculosis in Java, Indonesia. Methodology. Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared. Results. Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS–MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33–76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population. Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.

Study of antibiotics resistance in bacteria isolated from retailed eggshell in three major markets in Minna, Nigeria

2020 ◽  
pp. 3709-3719
Author(s):  
N.U. Adabara ◽  
C.E. Amarachi ◽  
A.S. Adedeji ◽  
A. Usman ◽  
M. Maude ◽  
...  
Keyword(s):  
Public Health ◽  
Public Health Problem ◽  
Developed Countries ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Major Public Health Problem ◽  
Antibiotic Susceptibility Test

Food borne disease associated with inappropriately treated or untreated eggs is a major public health problem affecting developing and developed countries. This study isolated, identified and determined the Antibiogram of bacteria isolates from eggshell of eggs obtained from three major markets in Minna, Nigeria. A total of ten (10) duplicate egg samples were purchased from egg retailers. The egg samples were analyzed for the enumeration and isolation of bacteria. The isolated bacteria were identified using biochemical methods. Antibiotic susceptibility test to prescribed commercially available antibiotic discs was also investigated. The total viable bacteria count (TVBC) ranged from 1.38×104 - 2.52×104 cfu/mL while the total coliform count (TCC) ranged from 8.5×103 - 2.02×104 cfu/mL. Escherichia coli (9.1%), Streptococcus pyogenes (4.5%), Enterococcus sp. (4.5%), Shigella sp. (13.6%), Salmonella sp. (13.6%), Staphylococcus aureus (36.4%), Clostridium sp. (9.1%) and Neisseria sp. (9.1%) were isolated and identified from eggshells. Gram positive bacteria showed highest sensitivity to gentamycin (100%) however, resist cloxacillin, ceftadizime and erythromycin (100%). Similarly, there was no ciprofloxacin resistant Gram-negative bacteria though E. coli, Salmonella sp. and Shigella sp. isolated from eggshells were resistant to augmentin and amoxicillin. Further analysis of result revealed that all the isolated bacteria from eggshells were multidrug resistant except Neisseria sp. with multidrug resistant index greater than 0.2. The fact that these antibiotic resistant bacteria can be transferred to humans is of public health concern. Therefore, stringent use of public health regulations for cleaning eggs before retailing is advocated.

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