Identification of the Novel Lincosamide Resistance Genelnu(E) Truncated by ISEnfa5-cfr-ISEnfa5Insertion in Streptococcus suis:De NovoSynthesis and Confirmation of Functional Activity in Staphylococcus aureus
2013 ◽
Vol 58
(3)
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pp. 1785-1788
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ABSTRACTThe novel lincosamide resistance genelnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5segment, was identified inStreptococcus suis. The genelnu(E) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. Thelnu(E) gene and its promoter region werede novosynthesized, andStaphylococcus aureusRN4220 carrying a shuttle vector with the clonedlnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.