scholarly journals Characteristics of Carbapenemase-Producing Enterobacteriaceae in Wastewater Revealed by Genomic Analysis

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Ryota Gomi ◽  
Tomonari Matsuda ◽  
Masaki Yamamoto ◽  
Pei-Hsin Chou ◽  
Michio Tanaka ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Genomic Analysis ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Whole Genome Analysis ◽  
Content Type ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Encoding Genes

ABSTRACT Wastewater is considered a major source of antibiotic-resistant bacteria released into the environment. Here, we characterized carbapenemase-producing Enterobacteriaceae (CPE) in wastewater by whole-genome analysis. Wastewater samples ( n = 40) were collected from municipal wastewater treatment plants and hospital wastewater in Japan and Taiwan. Samples were screened for CPE using selective media, and the obtained isolates were sequenced using an Illumina MiSeq. The isolates ( n = 45) included the following microorganisms: Klebsiella quasipneumoniae ( n = 12), Escherichia coli ( n = 10), Enterobacter cloacae complex ( n = 10), Klebsiella pneumoniae ( n = 8), Klebsiella variicola ( n = 2), Raoultella ornithinolytica ( n = 1), Citrobacter freundii ( n = 1), and Citrobacter amalonaticus ( n = 1). Among the 45 isolates, 38 harbored at least one carbapenemase-encoding gene. Of these, the bla GES ( bla GES-5 , bla GES-6 , and bla GES-24 ) genes were found in 29 isolates. The genes were situated in novel class 1 integrons, but the integron structures were different between the Japanese (In1439 with bla GES-24 and In1440 with bla GES-5 ) and Taiwanese (In1441 with bla GES-5 and In1442 with bla GES-6 ) isolates. Other carbapenemase-encoding genes ( bla VIM-1 , bla NDM-5 , bla IMP-8 , bla IMP-19 , and bla KPC-2 ) were found in one to three isolates. Notably, class 1 integrons previously reported among clinical isolates obtained in the same regions as the present study, namely, In477 with bla IMP-19 and In73 with bla IMP-8 , were found among the Japanese and Taiwanese isolates, respectively. The results indicate that CPE with various carbapenemase-encoding genes in different genetic contexts were present in biologically treated wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.

scholarly journals Carbapenem-Hydrolyzing GES-5-Encoding Gene on Different Plasmid Types Recovered from a Bacterial Community in a Sewage Treatment Plant

2011 ◽  
Vol 78 (4) ◽  
pp. 1292-1295 ◽  
Author(s):  
Delphine Girlich ◽  
Laurent Poirel ◽  
Rafael Szczepanowski ◽  
Andreas Schlüter ◽  
Patrice Nordmann
Keyword(s):  
Bacterial Community ◽  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Municipal Wastewater Treatment ◽  
Content Type ◽  
Carbapenemase Gene ◽  
Class 1 ◽  
Encoding Gene

ABSTRACTPlasmids pRSB113 and pRSB115 were recovered from an activated sludge bacterial community of a municipal wastewater treatment plant in Germany. Both plasmids carry the sameblaGES-5carbapenemase gene, located within two distinct class 1 integrons. These plasmids have different backbones, belong to different incompatibility groups, and could replicate in bothPseudomonas aeruginosaandEscherichia coli.

scholarly journals On-Site Pilot Testing of Hospital Wastewater Ozonation to Reduce Pharmaceutical Residues and Antibiotic-Resistant Bacteria

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 684
Author(s):  
Sofia Svebrant ◽  
Robert Spörndly ◽  
Richard H. Lindberg ◽  
Therese Olsen Sköldstam ◽  
Jim Larsson ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
High Flow ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Untreated Wastewater ◽  
Significant Difference ◽  
Hospital Sewage

Hospital sewage constitutes an important point source for antibiotics and antibiotic-resistant bacteria due to the high antibiotic use. Antibiotic resistance can develop and cause problems in sewage systems within hospitals and municipal wastewater treatment plants, thus, interventions to treat hospital sewage on-site are important. Ozonation has proven effective in treating relatively clean wastewater, but the effect on untreated wastewater is unclear. Therefore, we piloted implementation of ozonation to treat wastewater in a tertiary hospital in Uppsala, Sweden. We measured active pharmaceutical ingredients (APIs) using liquid chromatography-mass spectrometry and antibiotic-resistant Enterobacteriaceae using selective culturing pre- and post-ozonation. Comparing low (1 m3/h) and high (2 m3/h) flow, we obtained a ‘dose-dependent’ effect of API reduction (significant reduction of 12/29 APIs using low and 2/29 APIs using high flow, and a mean reduction of antibiotics of 41% using low vs. 6% using high flow, 25% vs. 6% for all APIs). There was no significant difference in the amount of antibiotic-resistant Enterobacteiaceae pre- and post-ozonation. Our results demonstrate that ozonation of untreated wastewater can reduce API content. However, due to the moderate API decrease and numerous practical challenges in the on-site setting, this specific ozonation system is not suitable to implement at full scale in our hospital.

scholarly journals Prevalence of Antibiotic-Resistant Bacteria ESKAPE among Healthy People Estimated by Monitoring of Municipal Wastewater

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 495
Author(s):  
Masateru Nishiyama ◽  
Susan Praise ◽  
Keiichi Tsurumaki ◽  
Hiroaki Baba ◽  
Hajime Kanamori ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
General Population ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Antibiotic Resistant ◽  
Carbapenem Resistant ◽  
Wastewater Samples

There is increasing attention toward factors that potentially contribute to antibiotic resistance (AR), as well as an interest in exploring the emergence and occurrence of antibiotic resistance bacteria (ARB). We monitored six ARBs that cause hospital outbreaks in wastewater influent to highlight the presence of these ARBs in the general population. We analyzed wastewater samples from a municipal wastewater treatment plant (MWWTP) and hospital wastewater (HW) for six species of ARB: Carbapenem-resistant Enterobacteria (CARBA), extended-spectrum β-lactamase producing Enterobacteria (ESBL), multidrug-resistant Acinetobacter (MDRA), multidrug-resistant Pseudomonas aeruginosa (MDRP), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococci (VRE). We registered a high percentage of ARBs in MWWTP samples (>66%) for all ARBs except for MDRP, indicating a high prevalence in the population. Percentages in HW samples were low (<78%), and no VRE was detected throughout the study. CARBA and ESBL were detected in all wastewater samples, whereas MDRA and MRSA had a high abundance. This result demonstrated the functionality of using raw wastewater at MWWTP to monitor the presence and extent of ARB in healthy populations. This kind of surveillance will contribute to strengthening the efforts toward reducing ARBs through the detection of ARBs to which the general population is exposed.

scholarly journals Efficiency of municipal wastewater treatment with membrane bioreactor

2019 ◽  
Vol 41 (1) ◽  
pp. 47-54
Author(s):  
Magdalena Domańska ◽  
Anna Boral ◽  
Kamila Hamal ◽  
Magdalena Kuśnierz ◽  
Janusz Łomotowski ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Membrane Filtration ◽  
Municipal Wastewater ◽  
High Efficiency ◽  
Biological Membrane ◽  
Treatment Plant ◽  
Membrane Reactors ◽  
Quality Analysis ◽  
Treated Wastewater ◽  
Municipal Wastewater Treatment

AbstractThe increasingly stringent requirements for wastewater treatment enforce the adoption of technologies that reduce pollution and minimize waste production. By combining the typical activated sludge process with membrane filtration, biological membrane reactors (MBR) offer great technological potential in this respect. The paper presents the principles and effectiveness of using an MBR at the Głogów Małopolski operation. Physicochemical tests of raw and treated wastewater as well as microscopic analyses with the use of the FISH (fluorescence in situ hybridization) method were carried out. Moreover, the level of electric energy consumption during the operation of the wastewater treatment plant and problems related to fouling were also discussed. A wastewater quality analysis confirmed the high efficiency of removing organic impurities (on average 96% in case of BOD5 and 94% in case of COD) and suspension (on average 93%).

scholarly journals Molecular Basis of Sulfonamide and Trimethoprim Resistance in Fish-Pathogenic Aeromonas Isolates

2011 ◽  
Vol 77 (20) ◽  
pp. 7147-7150 ◽  
Author(s):  
Kristina Kadlec ◽  
Ellen von Czapiewski ◽  
Heike Kaspar ◽  
Jürgen Wallmann ◽  
Geovana Brenner Michael ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Molecular Basis ◽  
Aeromonas Salmonicida ◽  
The Novel ◽  
Content Type ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Novel Gene ◽  
Class 1 ◽  
Diseased Fish

ABSTRACTSulfonamide-trimethoprim-resistantAeromonas salmonicidaand motileAeromonasspp. from diseased fish of the GERM-Vet study carried thesul1gene together with mostly cassette-borne trimethoprim resistance genes, including the novel genedfrA28. The sevendfrAanddfrBgenes identified were located mostly in class 1 integrons which commonly harbored other gene cassettes.

Antibiotic Resistance and Characteristics of Integrons in Escherichia coli Isolated from Penaeus vannamei at a Freshwater Shrimp Farm in Zhejiang Province, China

2019 ◽  
Vol 82 (3) ◽  
pp. 470-478 ◽  
Author(s):  
HUI CHENG ◽  
HAN JIANG ◽  
JIEHONG FANG ◽  
CHENG ZHU
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Insertion Sequence ◽  
Zhejiang Province ◽  
Resistant Bacteria ◽  
Penaeus Vannamei ◽  
Shrimp Farm ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1

ABSTRACT Our study was conducted to investigate the antibiotic susceptibility profiles, integrons and their associated gene cassettes (GCs), and insertion sequence common regions of Escherichia coli isolates from Penaeus vannamei collected at a large-scale freshwater shrimp farm in Zhejiang Province, People's Republic of China. A total of 182 E. coli isolates were identified from 200 samples. With the exception of imipenem, isolates were most commonly resistant to β-lactams, followed by tetracylines and sulfonamides. Fifty-two (28.6%) E. coli isolates were classified as multidrug resistant, and the patterns were highly diverse, with 29 types represented. The multiple-antibiotic resistance indices of the isolates were 0.17 to 0.56; 9.3% (17) of the 182 isolates were positive for class 1 integrons, 0.5% (1 isolate) was positive for class 2 integrons, and an insertion sequence common region 1 element was found upstream of the intI1 (integrase) gene in one of the intI1-positive isolates. Four GC arrays were detected in class 1 integrons, and one GC array was detected in class 2 integrons. Although the overall prevalence of antimicrobial-resistant bacteria in P. vannamei was lower than that previously reported for poultry and livestock farms in China, concerns about the inappropriate use of antibiotics and the transmission of antimicrobial-resistant bacteria in aquaculture were raised. Alternative approaches to reducing or replacing the use of antibiotics should be further studied.

scholarly journals Bio-Methanol Production Using Treated Domestic Wastewater with Mixed Methanotroph Species and Anaerobic Digester Biogas

Water ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1414 ◽  
Author(s):  
I-Tae Kim ◽  
Young-Seok Yoo ◽  
Young-Han Yoon ◽  
Ye-Eun Lee ◽  
Jun-Ho Jo ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Cost Effective ◽  
Treated Wastewater ◽  
Culture Solution ◽  
Municipal Wastewater Treatment ◽  
Mineral Salts ◽  
Methanol Production ◽  
Municipal Wastewater Treatment Plants

The development of cost-effective methods, which generate minimal chemical wastewater, for methanol production is an important research goal. In this study, treated wastewater (TWW) was utilized as a culture solution for methanol production by mixed methanotroph species as an alternative to media prepared from commercial or chemical agents, e.g., nitrate mineral salts medium. Furthermore, a realistic alternative for producing methanol in wastewater treatment plants using biogas from anaerobic digestion was proposed. By culturing mixed methanotroph species with nitrate and phosphate-supplemented TWW in municipal wastewater treatment plants, this study demonstrates, for the first time, the application of biogas generated from the sludge digester of municipal wastewater treatment plants. NaCl alone inhibited methanol dehydrogenase and the addition of 40 mM formate as an electron donor increased methanol production to 6.35 mM. These results confirmed that this practical energy production method could enable cost-effective methanol production. As such, methanol produced in wastewater treatment plants can be used as an eco-friendly energy and carbon source for biological denitrification, which can be an alternative to reducing the expenses required for the waste water treatment process.

Occurrence and fate of relevant substances in wastewater treatment plants regarding Water Framework Directive and future legislations

2012 ◽  
Vol 65 (7) ◽  
pp. 1179-1189 ◽  
Author(s):  
S. Martin Ruel ◽  
J.-M. Choubert ◽  
H. Budzinski ◽  
C. Miège ◽  
M. Esperanza ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Water Framework Directive ◽  
Municipal Wastewater ◽  
River Flow ◽  
Wastewater Treatment Plants ◽  
Quality Standard ◽  
Treated Wastewater ◽  
Municipal Wastewater Treatment ◽  
Conventional Activated Sludge ◽  
Treatment Processes

The next challenge of wastewater treatment is to reliably remove micropollutants at the microgram per litre range. During the present work more than 100 substances were analysed through on-site mass balances over 19 municipal wastewater treatment lines. The most relevant substances according to their occurrence in raw wastewater, in treated wastewater and in sludge were identified, and their fate in wastewater treatment processes was assessed. About half of priority substances of WFD were found at concentrations higher than 0.1 μg/L in wastewater. For 26 substances, potential non-compliance with Environmental Quality Standard of Water Framework Directive has been identified in treated wastewater, depending on river flow. Main concerns are for Cd, DEHP, diuron, alkylphenols, and chloroform. Emerging substances of particular concern are by-products, organic chemicals (e.g. triclosan, benzothiazole) and pharmaceuticals (e.g. ketoprofen, diclofenac, sulfamethoxazole, carbamazepine). About 80% of the load of micropollutants was removed by conventional activated sludge plants, but about two-thirds of removed substances were mainly transferred to sludge.

scholarly journals Dissemination of Multiple Drug Resistance Genes by Class 1 Integrons in Klebsiella pneumoniae Isolates from Four Countries: a Comparative Study

2011 ◽  
Vol 55 (7) ◽  
pp. 3140-3149 ◽  
Author(s):  
Piklu Roy Chowdhury ◽  
Ana Ingold ◽  
Natasha Vanegas ◽  
Elena Martínez ◽  
John Merlino ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multiple Drug Resistance ◽  
Rapid Evolution ◽  
Multiple Drug ◽  
South American ◽  
Content Type ◽  
Class 1 Integrons ◽  
Genetic Elements ◽  
Class 1 ◽  
Comparative Genetic Analysis

ABSTRACTA comparative genetic analysis of 42 clinicalKlebsiella pneumoniaeisolates, resistant to two or more antibiotics belonging to the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on the genetic contexts of class 1 integrons, mobilizable genetic elements best known for their role in the rapid evolution of antibiotic resistance among Gram-negative pathogens. It was found that the class 1 integrons in this cohort were located in a number of different genetic contexts with clear regional differences. In Sydney, IS26-associated Tn21-like transposons on IncL/M plasmids contribute greatly to the dispersal of integron-associatedmultiple-drug-resistant (MDR) loci. In contrast, in the South American countries, Tn1696-like transposons on an IncA/C plasmid(s) appeared to be disseminating a characteristic MDR region. A range of mobile genetic elements is clearly being recruited by clinically important mobile class 1 integrons, and these elements appear to be becoming more common with time. This in turn is driving the evolution of complex and laterally mobile MDR units and may further complicate antibiotic therapy.

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