scholarly journals Novel Class A β-Lactamase Sed-1 fromCitrobacter sedlakii: Genetic Diversity of β-Lactamases within the Citrobacter Genus

2001 ◽  
Vol 45 (8) ◽  
pp. 2287-2298 ◽  
Author(s):  
Stephanie Petrella ◽  
Dominique Clermont ◽  
Isabelle Casin ◽  
Vincent Jarlier ◽  
Wladimir Sougakoff
Keyword(s):  
Dna Hybridization ◽  
Klebsiella Oxytoca ◽  
Transcriptional Regulator ◽  
Enterobacter Cloacae ◽  
Clinical Strain ◽  
Proteus Vulgaris ◽  
Class A ◽  
Gene Coding ◽  
Quinolone Antibiotics ◽  
Reference Strains

ABSTRACT Citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with β-lactam and quinolone antibiotics. The isolate produced an inducible class A β-lactamase of pI 8.6, named Sed-1, which was purified. Characterized by a molecular mass of 30 kDa, Sed-1 preferentially hydrolyzed benzylpenicillin, cephalothin, and cloxacillin. The corresponding gene,bla Sed-1, was cloned and sequenced. Its deduced amino acid sequence shared more than 60% identity with the chromosome-encoded β-lactamases from Citrobacter koseri(formerly C. diversus) (84%), Klebsiella oxytoca (74%), Serratia fonticola (67%), andProteus vulgaris (63%) and 71% identity with the plasmid-mediated enzyme MEN-1. A gene coding for a LysR transcriptional regulator was found upstream from bla Sed-1. This regulator, named SedR, displayed 90% identity with the AmpR sequence of the chromosomal β-lactamase from C. koseriand 63 and 50% identity with the AmpR sequences of P. vulgaris and Enterobacter cloacae, respectively. By using DNA-DNA hybridization, a bla Sed-1-like gene was identified in two reference strains, C. sedlakii(CIP-105037) and Citrobacter rodentium (CIP-104675), but not in the 18 strains of C. koseri studied. Two DNA fragments were amplified and sequenced from the reference strains ofC. sedlakii CIP-105037 and C. rodentiumCIP-104675 using two primers specific forbla Sed-1. They shared 98 and 80% identity withbla Sed-1, respectively, confirming the diversity of the chromosomally encoded class A β-lactamases found inCitrobacter.

scholarly journals Interactions of β-Lactamases with Sanfetrinem (GV 104326) Compared to Those with Imipenem and with Oral β-Lactams

1998 ◽  
Vol 42 (5) ◽  
pp. 1168-1175 ◽  
Author(s):  
Gioia S. Babini ◽  
Meifang Yuan ◽  
David M. Livermore
Keyword(s):  
Functional Group ◽  
Klebsiella Oxytoca ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Morganella Morganii ◽  
Full Activity ◽  
Class A ◽  
Extended Spectrum ◽  
Class B ◽  
Hydrolysis Of

ABSTRACT Sanfetrinem is a trinem β-lactam which can be administered orally as a hexatil ester. We examined whether its β-lactamase interactions resembled those of the available carbapenems, i.e., stable to AmpC and extended-spectrum β-lactamases but labile to class B and functional group 2f enzymes. The comparator drugs were imipenem, oral cephalosporins, and amoxicillin. MICs were determined for β-lactamase expression variants, and hydrolysis was examined directly with representative enzymes. Sanfetrinem was a weak inducer of AmpC β-lactamases below the MIC and had slight lability, with ak cat of 0.00033 s−1 for theEnterobacter cloacae enzyme. Its MICs for AmpC-derepressedE. cloacae and Citrobacter freundii were 4 to 8 μg/ml, compared with MICs of 0.12 to 2 μg/ml for AmpC-inducible and -basal strains; MICs for AmpC-derepressed Serratia marcescens and Morganella morganii were not raised. Cefixime and cefpodoxime were more labile than sanfetrinem to theE. cloacae AmpC enzyme, and AmpC-derepressed mutants showed much greater resistance; imipenem was more stable and retained full activity against derepressed mutants. Like imipenem, sanfetrinem was stable to TEM-1 and TEM-10 enzymes and retained full activity against isolates and transconjugants with various extended-spectrum TEM and SHV enzymes, whereas these organisms were resistant to cefixime and cefpodoxime. Sanfetrinem, like imipenem and cefixime but unlike cefpodoxime, also retained activity against Proteus vulgaris and Klebsiella oxytoca strains that hyperproduced potent chromosomal class A β-lactamases. Functional group 2f enzymes, including Sme-1, NMC-A, and an unnamed enzyme fromAcinetobacter spp., increased the sanfetrinem MICs by up to 64-fold. These enzymes also compromised the activities of imipenem and amoxicillin but not those of the cephalosporins. The hydrolysis of sanfetrinem was examined with a purified Sme-1 enzyme, and biphasic kinetics were found. Finally, zinc β-lactamases, including IMP-1 and the L1 enzyme of Stenotrophomonas maltophilia, conferred resistance to sanfetrinem and all other β-lactams tested, and hydrolysis was confirmed with the IMP-1 enzyme. We conclude that sanfetrinem has β-lactamase interactions similar to those of the available carbapenems except that it is a weaker inducer of AmpC types, with some tendency to select derepressed mutants, unlike imipenem and meropenem.

Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Providencia Rettgeri ◽  
E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

scholarly journals Characterization of SFO-1, a Plasmid-Mediated Inducible Class A β-Lactamase from Enterobacter cloacae

1999 ◽  
Vol 43 (2) ◽  
pp. 307-313 ◽  
Author(s):  
Yoshimi Matsumoto ◽  
Matsuhisa Inoue
Keyword(s):  
Klebsiella Oxytoca ◽  
Enterobacter Cloacae ◽  
Gram Negative Bacteria ◽  
Phenotypic Characteristics ◽  
Gene Encoding ◽  
E Coli ◽  
Class A ◽  
Citrobacter Diversus ◽  
High Degree

ABSTRACT Enterobacter cloacae 8009 produced an inducible class A β-lactamase which hydrolyzed cefotaxime efficiently. It also hydrolyzed other β-lactams except cephamycins and carbapenems. The activity was inhibited by clavulanic acid and imipenem. Thebla gene was transferable to Escherichia coliby electroporation of plasmid DNA. The molecular mass of the β-lactamase was 29 kDa and its pI was 7.3. All of these phenotypic characteristics of the enzyme except for inducible production resemble those of some extended-spectrum class A β-lactamases like FEC-1. The gene encoding this β-lactamase was cloned and sequenced. The deduced amino acid sequence of the β-lactamase was homologous to the AmpA sequences of the Serratia fonticola chromosomal enzyme (96%), MEN-1 (78%), Klebsiella oxytoca chromosomal enzymes (77%), TOHO-1 (75%), and FEC-1 (72%). The conserved sequences of class A β-lactamases, including the S-X(T)-X(S)-K motif, in the active site were all conserved in this enzyme. On the basis of the high degree of homology to the β-lactamase of S. fonticola, the enzyme was named SFO-1. The ampR gene was located upstream of the ampA gene, and the AmpR sequence of SFO-1 had homology with the AmpR sequences of the chromosomal β-lactamases from Citrobacter diversus(80%), Proteus vulgaris (68%), and Pseudomonas aeruginosa (60%). SFO-1 was also inducible in E. coli. However, a transformant harboring plasmid without intactampR produced a small amount of β-lactamase constitutively, suggesting that AmpR works as an activator ofampA of SFO-1. This is the first report from Japan describing an inducible plasmid-mediated class A β-lactamase in gram-negative bacteria.

scholarly journals Postneurosurgical Meningitis Due to Proteus penneri with Selection of a Ceftriaxone-Resistant Isolate: Analysis of Chromosomal Class A β-Lactamase HugA and its LysR-Type Regulatory Protein HugR

2002 ◽  
Vol 46 (1) ◽  
pp. 216-219 ◽  
Author(s):  
Nadia Liassine ◽  
Stéphanie Madec ◽  
Béatrice Ninet ◽  
Catherine Metral ◽  
Martine Fouchereau-Peron ◽  
...  
Keyword(s):  
Regulatory Protein ◽  
Transcriptional Regulator ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistant Isolate ◽  
Proteus Vulgaris ◽  
Class A ◽  
Lactamase Gene ◽  
Proteus Penneri ◽  
Selection Of ◽  
Lysr Family

ABSTRACT We report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible Proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. The isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single β-lactamase named HugA with an isoelectric point of 6.7. The ceftriaxone-resistant isolate hyperproduced the β-lactamase (increase in the level of production, about 90-fold). The sequences of the hugA β-lactamase gene and its regulator, hugR, were identical in both P. penneri strains and had 85.96% homology with those of Proteus vulgaris. The HugA β-lactamase belongs to molecular class A, and the transcriptional regulator HugR belongs to the LysR family.

scholarly journals IBC-1, a Novel Integron-Associated Class A β-Lactamase with Extended-Spectrum Properties Produced by anEnterobacter cloacae Clinical Strain

2000 ◽  
Vol 44 (9) ◽  
pp. 2247-2253 ◽  
Author(s):  
Panagiota Giakkoupi ◽  
Leonidas S. Tzouvelekis ◽  
Athanassios Tsakris ◽  
Veneta Loukova ◽  
Danai Sofianou ◽  
...  
Keyword(s):  
Amino Acid ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Clinical Strain ◽  
Nucleotide Sequence Analysis ◽  
Sequence Comparisons ◽  
Class 1 Integron ◽  
Class A ◽  
Species Specific

ABSTRACT A transferable β-lactamase produced by a multidrug-resistant clinical isolate of Enterobacter cloacae was studied. Thebla gene was carried by a large (>80-kb) transmissible plasmid. Nucleotide sequence analysis of cloned fragments revealed that it was part of a gene cassette carried by a class 1 integron along with other resistance genes, includingaac(6′)-Ib. The encoded β-lactamase, designated IBC-1, was a novel class A enzyme that hydrolyzed ceftazidime and cefotaxime and was inhibited by tazobactam and, to a lesser extent, by clavulanate. Also, imipenem exhibited potent inhibitory activity against IBC-1. The enzyme consisted of 287 amino acid residues, including Ser-237, cysteines at positions 69 and 237a, and Arg-244, which may be implicated in its interaction with β-lactams. In amino acid sequence comparisons, IBC-1 displayed the highest similarity with the chromosomal penicillinase of Yersinia enterocolitica, a carbenicillinase from Proteus mirabilis GN79, the species-specific β-lactamases ofKlebsiella oxytoca, and the carbapenemase Sme-1. However, a phylogenetic association with established β-lactamase clusters could not be conclusively shown.

scholarly journals Assessment of Health Risks Related to the Consumption of Minced Meat Sandwich

2020 ◽  
Vol 9 (1) ◽  
pp. 30
Author(s):  
Alain B. Vouidibio Mbozo ◽  
Christian A. Kayath ◽  
Saturnin N. Mokémiabéka ◽  
Etienne Nguimbi
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Health Risks ◽  
Informal Sector ◽  
Klebsiella Oxytoca ◽  
Enterobacter Cloacae ◽  
Resistance Testing ◽  
Proteus Vulgaris ◽  
The Republic ◽  
Minced Meat

The objective of this work was to assess the health risks associated with the consumption of minced meat sandwiches, sold in the informal sector in Brazzaville in the Republic of Congo. A survey on the application of hygiene rules was conducted in parallel with a bacteriological analysis of cooked minced meat. The enterobacteria isolated from this food were identified and antibiotic resistance testing was performed. The investigation revealed shortcomings in respect of basic hygiene rules, and 56% of the sandwiches analyzed were of bacteriological quality unsatisfactory. The non-compliance of the sandwiches was caused mainly by the presence total aerobic mesophilic flora (71.43%) and total coliforms (57.14%). In contrast, not all samples were contaminated with anaerobes sulfito-reducting bacteria and Salmonella. Five species of Enterobacteriaceae were identified: Escherichia coli (35.30%), Proteus vulgaris (11.76%), Klebsiella oxytoca (11.76%), Citrobacter spp. (23.53%) and Enterobacter cloacae (17.65%). Of these, 42.65% were resistant to 75% of antibiotics tested: Cefalexin (17.24%), Ceftriaxone (48.28%) and Norfloxacin (34.48%). In contrast, all strains were sensitive to Nitrofurantoin. Minced meat sandwiches sold in informal sector in Brazzaville can be source of enteropathogens, susceptible to expose consumers to foods poisonings.

scholarly journals Microbiology of brewing production - bacteria of the order Enterobacterales and culture methods for their detection

2020 ◽  
Vol 66 (5) ◽  
pp. 345-350
Author(s):  
Petra Kubizniaková ◽  
Martina Brožová ◽  
Kateřina Štulíková ◽  
Eva Vontrobová ◽  
Katarína Hanzalíková ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Serratia Marcescens ◽  
Klebsiella Oxytoca ◽  
Enterobacter Cloacae ◽  
Citrobacter Freundii ◽  
Culture Methods ◽  
Aerobic Conditions ◽  
Rahnella Aquatilis ◽  
Raoultella Terrigena ◽  
Incubation Temperatures

The growth of 7 strains belonging to the order of Enterobacterales, represented by the species of Citrobacter Freundii, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Obesumbacterium proteus, Rahnella aquatilis, Raoultella terrigena, Serratia marcescens and Shimwellia pseudoproteus, was monitored on selected cultivation media. Three types of agars - Endo, MacConkey and Chromocult Coliform agar together with two incubation temperatures of 28 and 37 °C were tested under aerobic conditions. The aim of the study was to detect such essential enterobacteria harmful to beer that cannot be proven at 37 °C, which is the temperature usually used in operational laboratories in breweries. Our results showed that most of the tested strains of enterobacteria were able to grow at 28 °C on all selected types of agar. The exception was just the representatives detection of which is problematic at 37 °C. Nevertheless, a little or no growth was always observed on just one of the tested media.

scholarly journals Stenotrophomonas africana Drancourt et al. 1997 is a later synonym of Stenotrophomonas maltophilia (Hugh 1981) Palleroni and Bradbury 1993

2004 ◽  
Vol 54 (4) ◽  
pp. 1235-1237 ◽  
Author(s):  
Tom Coenye ◽  
Elke Vanlaere ◽  
Enevold Falsen ◽  
Peter Vandamme
Keyword(s):  
Dna Hybridization ◽  
Stenotrophomonas Maltophilia ◽  
Biochemical Characterization ◽  
Cell Protein ◽  
Protein Patterns ◽  
Whole Cell ◽  
Sds Page ◽  
Binding Level ◽  
Reference Strains ◽  
Type Strains

Type and reference strains of Stenotrophomonas maltophilia and Stenotrophomonas africana were compared with each other and with the type strains of other Stenotrophomonas species, using SDS-PAGE of whole-cell proteins, DNA–DNA hybridization and extensive biochemical characterization. S. maltophilia LMG 958T and S. africana LMG 22072T had very similar whole-cell-protein patterns and were also biochemically very similar. A DNA–DNA binding level of 70 % between both type strains confirmed that S. africana and S. maltophilia represent the same taxon. It is concluded that S. africana is a later synonym of S. maltophilia.

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