scholarly journals Mixed-Valence Cytoplasmic Iron Granules Are Linked to Anaerobic Respiration

2006 ◽  
Vol 73 (3) ◽  
pp. 993-996 ◽  
Author(s):  
S. Glasauer ◽  
S. Langley ◽  
M. Boyanov ◽  
B. Lai ◽  
K. Kemner ◽  
...  
Keyword(s):  
Ferrous Iron ◽  
Metal Particle ◽  
Ferric Iron ◽  
Solid Phase ◽  
Mixed Valence ◽  
Anaerobic Respiration ◽  
In Situ Detection ◽  
Relationship Of ◽  
The Relationship

ABSTRACT Intracellular granules containing ferric and ferrous iron formed in Shewanella putrefaciens CN32 during dissimilatory reduction of solid-phase ferric iron. It is the first in situ detection at high resolution (150 nm) of a mixed-valence metal particle residing within a prokaryotic cell. The relationship of the internal particles to Fe(III) reduction may indicate a respiratory role.

Open innovation practices adopted by private stakeholders: perspectives for living labs

Info ◽  
2015 ◽  
Vol 17 (4) ◽  
pp. 67-80 ◽  
Author(s):  
Dominic Lapointe ◽  
David Guimont
Keyword(s):  
Open Innovation ◽  
Research Literature ◽  
Extensive Literature ◽  
Content Type ◽  
Living Lab ◽  
Living Labs ◽  
Innovation Practices ◽  
Relationship Of ◽  
The Relationship

Purpose – This paper aims to explore the role of private stakeholders in the living lab (LL) ecosystem and the relationship of private stakeholders to open innovation (OI) practices. There is extensive literature on private stakeholders and OI, but seldom mention is made on the specific question of how private stakeholders integrate OI in the context of a LL. Design/methodology/approach – The authors will analyze qualitatively how private businesses that have participated in a in situ open innovation evaluate and perceived their open innovation practices. Therefore, how they relate to open innovation. Then, the authors will identify a typology of the businesses in relation to OI. Findings – The research focused on the relationship of private stakeholders to OI in the context of in situ OI activities. The results obtained are consistent with literature on OI (Chesbrough, 2003). However, there are differences: if the elements mentioned by the respondents are described in literature, their representation of OI and its components allows us to affirm that this practice is not generalised and that it is often open to interpretation. That emphasises the importance of the role LLs can play as intermediaries to accompany private stakeholders in the OI process. Private stakeholders look for a guide to develop their OI know-how and find their way in the OI ecosystem. Originality/value – The value of this paper is to bridge the research on OI done with private organisation and the research on LLs. The research literature did not pay much attention to the representation of the private stakeholders in the OI ecosystem. This paper has provided the start to open up that field.

scholarly journals EFFECT OF BLASTING ON THE STABILITY OF LINING DURING EXCAVATION OF NEW TUNNEL NEAR THE EXISTING TUNNEL

2021 ◽  
Vol 30 (1) ◽  
Author(s):  
Tuan Minh Tran ◽  
Quang Huy Nguyen
Keyword(s):  
Field Data ◽  
Damage Zone ◽  
Field Tests ◽  
Surrounding Rock ◽  
In Situ Test ◽  
The Stability ◽  
Relationship Of ◽  
The Impact ◽  
The Relationship

In recent years, experimental and numerical researches on the effect of blasting pressure on the stability of existing tunnels was widely obtained. However, the effect of the blasting pressure during excavation a new tunnel or expansion old tunnels on an existing tunnel has disadvantages and still unclear. Some researches were carried out to study the relationship of the observed Peak Particle Velocity (PPV) on the lining areas along the existing tunnel direction, due to either the lack of in situ test data or the difficulty in conducting field tests, particularly for tunnels that are usually old and vulnerable after several decades of service. This paper introduces using numerical methods with the field data investigations on the effect of the blasting in a new tunnel on the surrounding rock mass and on the existing tunnel. The research results show that not only predicting the tunnel lining damage zone under the impact of blast loads but also determination peak maximum of explosion at the same time at the surface of tunnel working.

scholarly journals Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice: demonstration by in situ hybridization

Blood ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 993-998 ◽  
Author(s):  
T Jippo ◽  
H Mizuno ◽  
Z Xu ◽  
S Nomura ◽  
M Yamamoto ◽  
...  
Keyword(s):  
Mast Cell ◽  
In Situ Hybridization ◽  
Mast Cells ◽  
Differentiation Markers ◽  
Two Parameters ◽  
High Affinity Ige Receptor ◽  
Relationship Of ◽  
The Relationship ◽  
Receptor Beta

Although GATA-binding transcription factors (GATA-1 and GATA-2) are strongly expressed in cultured mast cells (CMCs), their expression in mast cells within tissues has not been reported. We examined the expression of GATA-1 and GATA-2 in skin tissues of mice using Northern blot analysis and in situ hybridization. mRNA for GATA-2 but not for GATA-1 was expressed in skin mast cells of WB-+/+ embryos between days 15 and 17 postcoitum (pc). The expression was downregulated on and after day 18 pc. Skin mast cells did not express GATA-2 after birth either. When the number of skin mast cells was compared with the number of GATA-2 mRNA-expressing cells, GATA-2 mRNA appeared to be expressed by mast cells only when the number was increasing. When the mRNA expression of high-affinity IgE receptor beta-subunit and mast cell carboxypeptidase A was used as differentiation markers, the expression of these mRNAs continued even after the downregulation of GATA-2 expression. To clarify the relationship of the proliferation and GATA-2 expression, proliferating CMCs derived from WBB6F1-+/+ mice were transplanted into the peritoneal cavity of mast cell-deficient WBB6F1- W/Wv mice. The CMCs stopped both the proliferation and GATA-2 expression after the transplantation, suggesting the association of these two parameters in mast cells within tissues of mice.

Experiment Research and Design of a Field Test Device for Permeability of Pore Air of Unsaturated Soils

2012 ◽  
Vol 170-173 ◽  
pp. 256-260
Author(s):  
Song Xu ◽  
Fu You Zhang ◽  
Jun Pin Yuan ◽  
Hong Wei Chen ◽  
Ming Gu
Keyword(s):  
Field Test ◽  
Unsaturated Soil ◽  
Unsaturated Soils ◽  
Test Field ◽  
Experiment Research ◽  
Test Device ◽  
Relationship Of ◽  
The Relationship ◽  
Research And Design

Permeability of pore air is one of the main basic characters of unsaturated soil. Measuring it accurately is important in anti-seepage design. In this paper, a new kind of device was invented that could test permeability of pore air of unsaturated soil in situ quickly and accurately. With this apparatus, the relationship of air permeability coefficient with various dry densities and saturation of ground soils were obtained in a reservoir. Compared with routine indoor test, field test can decrease the disturbance to the soil and the result is more accurately.

Diaphragmatic thickness-lung volume relationship in vivo

1989 ◽  
Vol 67 (4) ◽  
pp. 1560-1568 ◽  
Author(s):  
J. L. Wait ◽  
P. A. Nahormek ◽  
W. T. Yost ◽  
D. P. Rochester
Keyword(s):  
Lung Volume ◽  
In Vivo Studies ◽  
Normal Male ◽  
Diaphragm Thickness ◽  
Diaphragmatic Function ◽  
Volume Relationship ◽  
Relationship Of ◽  
The Relationship

To characterize the relationship of changes in diaphragmatic thickness during contraction to changes in lung volume, we developed a technique to measure diaphragm thickness based on M-mode ultrasonography. First, diaphragmatic thickness was measured in situ at necropsy with ultrasound and verified by measuring the same resected segment of diaphragm by ruler (correlation coefficient = 0.93, slope = 0.97). The technique of imaging the diaphragm in living subjects was developed by using a 15-MHz transducer coupled to an M-mode echocardiograph. Ten normal male volunteers were studied while sitting. The ultrasound transducer was held between the ribs in the ninth lateral interspace, and tidal volume was measured by spirometry. The thickening fraction (TF) was calculated as TF = (thickness at peak inspiration - thickness at end expiration)/thickness at end expiration for each of a series of different sized breaths. The function, TF vs. lung volume, for a range of volumes was linear for each subject and had intrasubject reproducibility with intersubject variability. We conclude that diaphragmatic TF is related to function as determined by lung volume, and this may prove to be a useful technique for in vivo studies of diaphragmatic function.

scholarly journals Pengaruh Perbedaan Kedalaman Terhadap Fitoplankton dan Zooplankton Di Perairan Pulau Panjang, Jepara

2019 ◽  
Vol 8 (2) ◽  
pp. 181-188
Author(s):  
Deftika Mulyawati ◽  
Raden Ario ◽  
Ita Riniatsih
Keyword(s):  
Sea Level ◽  
Sea Water ◽  
Mesh Size ◽  
Active Method ◽  
Limited Mobility ◽  
Relationship Of ◽  
The Relationship ◽  
Ship Speed

Plankton merupakan sebuah kelompok organisme yang hanyut bebas di dalam lautan. Plankton dapat dibagi menjadi dua golongan, yaitu fitoplankton dan zooplankton. Penelitian ini bertujuan untuk mengetahui hubungan dari kelimpahan fitoplankton dan zooplankton berdasarkan perbedaan kedalaman di perairan timur Pulau Panjang Kabupaten Jepara. Pengambilan sampel dilakukan dengan metode aktif yaitu dengan menarik plankton net dengan mata jaring ukuran 45µ untuk fitoplankton dan 150µ untuk zooplankton. Penarikan dilakukan menggunakan kapal selama 3-5 menit dengan kecepatan kapal 0,67 m/s pada lokasi penelitian. Penentuan stasiun penelitian berdasarkan pada perbedaan kedalaman. Kedalaman air laut yang digunakan pada saat pengambilan sampel ini adalah stasiun 1 dengan kedalaman 0 m atau permukaan air laut, stasiun 2 dengan kedalaman 1-2 m dari permukaan air laut, dan stasiun 3 dengan kedalaman 3-4 m dari permukaan air laut. Pengukuran kualitas perairan dilakukan secara in situ bersamaan dengan pengambilan sampel dilakukan. Hasil dari penelitian ini didapatkan komposisi fitoplankton sebanyak 13 genus dengan kelimpahan berkisar antara 3844,19-10869,80 individu/m3. Komposisi zooplankton di perairan didapatkan sebanyak 12 genus dengan kelimpahan berkisar antara 838,77-3250,24 individu/m3. Hubungan kelimpahan antara zooplankton dan fitoplankton di perairan ini termasuk kedalam golongan hubungan korelasi positif Plankton is a group that drifts freely in the ocean. Plankton has limited mobility. Plankton can be divided into two groups, namely phytoplankton and zooplankton. This study aims to determine the relationship of the abundance of phytoplankton and zooplankton based on rates in the east of Panjang Island, Jepara Regency. Sampling is done by the active method, namely by pulling the plankton net with a mesh size of 45µ for phytoplankton and 150µ for zooplankton. Withdrawals carried out by a ship for 3-5 minutes with a ship speed of 0.67 m/s at the study site. Determination of research stations based on differences in depth. The depth of sea water used during sampling is station 1 with a depth of 0 m or sea level, station 2 with a depth of 1-2 m from sea level, and station 3 with a depth of 3-4 m above sea level. Measurements of water quality were carried out in situ together with sampling. The results of this study obtained phytoplankton compositions as many as 13 genera with abundance ranging from 3844.19-10869.80 individuals/m3. The composition of zooplankton in the waters obtained as many as 12 genera with abundance ranging from 838.77-3250.24 individuals/m3. The relationship of abundance between zooplankton and phytoplankton in these waters is included in the group of positive correlation.

scholarly journals Detection of Volatiles from Raw Beef Meat from Different Packaging Systems Using Solid-Phase Microextraction GC–Accurate Mass Spectrometry

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2018
Author(s):  
Debarati Bhadury ◽  
Yada Nolvachai ◽  
Philip J. Marriott ◽  
Joanne Tanner ◽  
Kellie L. Tuck
Keyword(s):  
Mass Spectrometry ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Carbon Disulphide ◽  
Accurate Mass ◽  
Flight Mass Spectrometry ◽  
Modified Atmospheric Packaging ◽  
Mass Quadrupole ◽  
Relationship Of ◽  
The Relationship

The volatile profile of raw beef contains vital information related to meat quality and freshness. This qualitative study examines the effect of packaging system on the formation and release of volatile organic compounds (VOCs) from raw beef over time, relative to the packaging best before date (BBD). The three packaging systems investigated were modified atmospheric packaging, vacuum packaging, and cling-wrapped packaging. Porterhouse steak samples with the same BBD were analysed from 3 days before to 3 days after the BBD. VOCs were detected via preconcentration using solid-phase microextraction combined with gas chromatography–accurate mass quadrupole time-of-flight mass spectrometry. In total, 35 different VOCs were tentatively identified. Interestingly, there was no clear relationship of the VOCs detected between the three packaging systems, with only carbon disulphide and acetoin, both known volatiles of beef, detected in all three. This is the first study to investigate the effects of commercial packaging systems on VOC formation; it provides an understanding of the relationship of VOCs to the BBD that is essential for the development of on-pack freshness and quality sensors.

scholarly journals Karakteristik Morfologi Lamun Thalassodendron ciliatum (Forsskall) Hartog 1970 (Kelas: Magnoliopsida,Famili : Cymodoceaceae) Berdasarkan Tipe Substrat di Perairan Pantai Timur Kabupaten Bulukumba

2020 ◽  
Vol 23 (1) ◽  
pp. 85
Author(s):  
Chair Rani ◽  
Muhammad Basri ◽  
Devi Yulianti Bahar ◽  
Meggy Yolanda
Keyword(s):  
Root Diameter ◽  
Weak Current ◽  
Sandy Substrate ◽  
Leaf Character ◽  
Number Of Leaves ◽  
Thalassodendron Ciliatum ◽  
Relationship Of ◽  
The Relationship ◽  
Measured Water

This study aims to determine the seagrass morphological characteristic based on substrates and analyze the relationship of environmental factors with the morphology of Thalassodendron ciliatum. A sampling at each station was done purposively according to the substrate. Sampling was carried out in 3 areas as replication by digging and taking seagrass complete with rhizome and its roots as many as 25 stands. Then the seagrass was cleaned from the sand and put into a sample bag and stored in a cooler. At the same time, sand and water samples were also taken to measure the nitrate and phosphate. The measured water quality in situ includes current speed, temperature, salinity, depth, and wave height. In the laboratory, measurements of morphological parameters of seagrass were done on the leaves, stems, rhizomes, and roots. The results showed that the sandy substrate (Kasuso Station: more protected) showed a longer and thicker leaf character, and a longer root with a greater number of roots, but had a number of leaves, rhizoma length, internode distance, and root diameter smaller than the area with the substrate was dominated by coral fragments (Panrangluhu Station). Under weak current conditions with high nitrate and phosphate content, they were characterized by the larger stem and leaf lengths with a large number of roots. Whereas in strong currents, seagrass was characterized by a greater number and area of leaves and root diameter. Areas with high waves, characterized by seagrasses that have larger stem diameters, longer rhizomes with larger diameters, and also longer internode distances Penelitian ini bertujuan untuk mendeterminasi karakter morfologi lamun berdasarkan tipe substrat dan menganalisis keterkaitan faktor lingkungan dengan morfologi lamun Thalassodendron ciliatum. Sampling pada setiap stasiun dilakukan secara purposif dengan memperhatikan susbstrat dasar.  Sampling dilakukan pada 3 area sebagai ulangan dengan  cara menggali dan mengambil lamun lengkap dengan rhizoma dan akarnya sebanyak 25 tegakan. Kemudian lamun dibersihkan dari pasir dan dimasukkan ke dalam kantong sampel yang berisi air laut dan disimpan dalam kotak pendingin.  Bersamaan dengan itu, juga diambil sampel pasir dan air untuk diukur kandungan nitrat dan posfatnya.  Kualitas air yang diukur secara insitu meliputi kecepatan arus, suhu, salinitas, kedalaman, dan tinggi gelombang.  Di laboratorium diukur parameter morfologi lamun pada bagian daun, batang, rhizoma, dan akar.  Hasil analisis menunjukkan bahwa pada substrat berpasir (Stasiun Kasuso: lebih terlindung) memperlihatkan karakter daun yang lebih panjang dan tebal, serta akar yang lebih panjang dengan jumlah akar yang lebih banyak, namun memiliki jumlah daun, panjang rhizoma, jarak internode, dan diameter akar yang lebih kecil dibandingkan dengan daerah yang substratnya didominasi oleh pecahan karang (Stasiun Panrangluhu). Pada kondisi arus yang lemah dengan kandungan nitrat dan posfat yang tinggi, dicirikan oleh panjang batang dan daun yang lebih besar dengan jumlah akar yang banyak. Sedangkan pada arus yang kuat, lamun dicirikan oleh jumlah dan luasan daun serta diameter akar yang lebih besar.  Daerah dengan gelombang yang tinggi, dicirikan oleh lamun yang memiliki diameter batang yang lebih besar, rhizoma yang lebih panjang dengan diameter yang besar, dan jarak internode yang juga lebih panjang.

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