scholarly journals Antibiotic-ResistantEscherichia coliand Class 1 Integrons in Humans, Domestic Animals, and Wild Primates in Rural Uganda

2018 ◽  
Vol 84 (21) ◽  
Author(s):  
Debora Weiss ◽  
Ryan M. Wallace ◽  
Innocent B. Rwego ◽  
Thomas R. Gillespie ◽  
Colin A. Chapman ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
National Parks ◽  
National Park ◽  
Nonhuman Primates ◽  
Domestic Animals ◽  
Content Type ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Resistance Patterns ◽  
Western Uganda

ABSTRACTAntibiotic resistance is a global concern, although it has been studied most extensively in developed countries. We studiedEscherichia coliand class 1 integrons in western Uganda by analyzing 1,685 isolates from people, domestic animals, and wild nonhuman primates near two national parks. Overall, 499 isolates (29.6%) were resistant to at least one of 11 antibiotics tested. The frequency of resistance reached 20.3% of isolates for trimethoprim-sulfamethoxazole but was nearly zero for the less commonly available antibiotics ciprofloxacin (0.4%), gentamicin (0.2%), and ceftiofur (0.1%). The frequency of resistance was 57.4% in isolates from people, 19.5% in isolates from domestic animals, and 16.3% in isolates from wild nonhuman primates. Isolates of livestock and primate origin displayed multidrug resistance patterns identical to those of human-origin isolates. The percentage of resistant isolates in people was higher near Kibale National Park (64.3%) than near Bwindi Impenetrable National Park (34.6%), perhaps reflecting local socioeconomic or ecological conditions. Across antibiotics, resistance correlated negatively with the local price of the antibiotic, with the most expensive antibiotics (nalidixic acid and ciprofloxacin) showing near-zero resistance. Among phenotypically resistant isolates, 33.2% harbored class 1 integrons containing 11 common resistance genes arranged into nine distinct gene cassettes, five of which were present in isolates from multiple host species. Overall, these results show that phenotypic resistance and class 1 integrons are distributed broadly amongE. coliisolates from different host species in this region, where local socioeconomic and ecological conditions may facilitate widespread diffusion of bacteria or resistance-conferring genetic elements.IMPORTANCEAntibiotic resistance is a global problem. This study, conducted in rural western Uganda, describes antibiotic resistance patterns inEscherichia colibacteria near two forested national parks. Resistance was present not only in people, but also in their livestock and in nearby wild nonhuman primates. Multidrug resistance and class 1 integrons containing genes that confer resistance were common and were similar in people and animals. The percentage of resistant isolates decreased with increasing local price of the antibiotic. Antibiotic resistance in this setting likely reflects environmental diffusion of bacteria or their genes, perhaps facilitated by local ecological and socioeconomic conditions.

Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 160-166
Author(s):  
Seyedeh Hanieh Eshaghi Zadeh ◽  
Hossein Fahimi ◽  
Fatemeh Fardsanei ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Food Borne ◽  
Class 1 ◽  
Resistance Patterns ◽  
C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

scholarly journals Integron-Derived Aminoglycoside-Sensing Riboswitches Control Aminoglycoside Acetyltransferase Resistance Gene Expression

2019 ◽  
Vol 63 (6) ◽  
Author(s):  
Shasha Wang ◽  
Weizhi He ◽  
Wenxia Sun ◽  
Jun Zhang ◽  
Yaowen Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Dna Recombination ◽  
Aminoglycoside Resistance ◽  
Content Type ◽  
Clinical Strains ◽  
Class 1 Integrons ◽  
Class 1

ABSTRACTClass 1 integrons accumulate antibiotic resistance genes by site-specific recombination ataatI-1sites. Captured genes are transcribed from a promoter located within the integron; for class 1 integrons, the first gene to be transcribed and translated normally encodes an aminoglycoside antibiotic resistance protein (either an acetyltransferase [AAC] or adenyltransferase [AAD]). The leader RNA from thePseudomonas fluorescensclass 1 integron contains an aminoglycoside-sensing riboswitch RNA that controls the expression of the downstream aminoglycoside resistance gene. Here, we explore the relationship between integron-dependent DNA recombination and potential aminoglycoside-sensing riboswitch products of recombination derived from a series of aminoglycoside-resistant clinical strains. Sequence analysis of the clinical strains identified a series of sequence variants that were associated with class I integron-derived aminoglycoside-resistant (bothaacandaad) recombinants. For theaacrecombinants, representative sequences showed up to 6-fold aminoglycoside-dependent regulation of reporter gene expression. Microscale thermophoresis (MST) confirmed RNA binding. Covariance analysis generated a secondary-structure model for the RNA that is an independent verification of previous models that were derived from mutagenesis and chemical probing data and that was similar to that of theP. fluorescensriboswitch RNA. The aminoglycosides were among the first antibiotics to be used clinically, and the data suggest that in an aminoglycoside-rich environment, functional riboswitch recombinants were selected during integron-mediated recombination to regulate aminoglycoside resistance. The incorporation of a functional aminoglycoside-sensing riboswitch by integron recombination confers a selective advantage for the expression of resistance genes of diverse origins.

Antimicrobial resistance patterns and characterization of integrons in clinical isolates of Shigella from China

2014 ◽  
Vol 60 (4) ◽  
pp. 237-242 ◽  
Author(s):  
Haifei Yang ◽  
Yachao Pan ◽  
Lifen Hu ◽  
Yanyan Liu ◽  
Ying Ye ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Agents ◽  
Wide Distribution ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Shigella Spp ◽  
Resistance Patterns ◽  
Resistance Rates

One hundred fifty-three Shigella isolates were examined for multiple antibiotic resistance phenotypes and prevalence of class 1 and class 2 integron sequences. The gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2, and arr-3-aacA4 were found in typical class 1 integrons. The gene cassettes blaOXA-1-aadA1 and dfrA1-sat1-aadA1 were detected in atypical class 1 integrons and in class 2 integrons, respectively. This is the first report of arr-3-aacA4 cassette detected in typical class 1 integrons among Shigella isolates. Rates of antibiotic resistance were different between integron-positive and integron-negative strains (P < 0.05), and all integron-positive isolates were resistant to at least 3 different antimicrobial agents. Typical class 1 integron-positive isolates showed higher resistance rates to cefotaxime and ciprofloxacin than did integron-negative ones (P < 0.05). Typical class 1 integrons and β-lactamase genes were found in conjugative plasmids, otherwise class 2 and atypical class 1 integrons were located on chromosome. This study demonstrated the wide distribution of class 1 integrons in Shigella spp., which may lead resistance to cefotaxime and ciprofloxacin in China.

scholarly journals Abundance of Multiple-Antibiotic-Resistant Salmonella Strains in Fecal Samples of Rhinoceros unicornis of the Kaziranga National Park, India

2021 ◽  
pp. 298-313
Author(s):  
Tilak Saha ◽  
Dibyendu Paul ◽  
Ranadhir Chakraborty
Keyword(s):  
Antibiotic Resistance ◽  
National Park ◽  
Antibiotic Resistant ◽  
Fecal Samples ◽  
Resistance Profile ◽  
Class 1 Integrons ◽  
Antibiotic Resistance Profile ◽  
Kaziranga National Park ◽  
Rhinoceros Unicornis ◽  
Class 1

Aims: This study was undertaken to assess the abundance of multiple-antibiotic-resistant (MAR) Salmonella strains in fecal samples of Rhinoceros unicornis of the Kaziranga National Park (KNP), India. Study Design: Antibiotic-resistance profile of the Salmonella isolates from fecal samples of rhinoceros was carried out by replica plating on Muller Hinton Agar (MHA) plates containing antibiotics. The presence of class 1 integrons in metallo-β-lactamase (MBL) producing Salmonella isolates was determined by multiplex PCR. Place and Duration of Study: The study was carried out on rhinos of KNP situated in the Golaghat district of Assam, India in April 2015. Methodology: Fresh rhino fecal samples (designated as 1R, 2R, 3R, 4R and 5R) were dilution plated onto MacConkey agar. Purified bacterial colonies were then streaked separately on bismuth sulphite (BS) agar plates. All black colonies which are characteric growth of Salmonella were selected and used to make master plates on Luria Agar. To determine the antibiotic-resistance profile of the isolates, master-plates of purified single colonies of Salmonella spp. were                  replicate-printed on plates containing antibiotics from the β-lactam, aminoglycoside, or quinolone groups. To detect the presence of an integron, a conserved segment polymerase chain reaction was used. Results: 97.6% of the Salmonella isolates tested were resistant to >1 antibiotics (multidrug resistant or MAR). A total of 100 isolates from two fecal samples, 4R and 5R, were found to be imipenem resistant; 52 of them tested positive for the presence of MBLs. Five of the twenty MBL producing strains contained class 1 integrons. Conclusion: Because Salmonella is usually spread by drinking contaminated water, it is likely that water bodies in KNP are contaminated with MAR Salmonella strains. In case of Salmonellosis outbreak among Indian one-horned rhinos, our antibiogram will assist the veterinarians to choose the appropriate regimen of antibiotics for the rhinos in the KNP.

scholarly journals Molecular Basis of Sulfonamide and Trimethoprim Resistance in Fish-Pathogenic Aeromonas Isolates

2011 ◽  
Vol 77 (20) ◽  
pp. 7147-7150 ◽  
Author(s):  
Kristina Kadlec ◽  
Ellen von Czapiewski ◽  
Heike Kaspar ◽  
Jürgen Wallmann ◽  
Geovana Brenner Michael ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Molecular Basis ◽  
Aeromonas Salmonicida ◽  
The Novel ◽  
Content Type ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Novel Gene ◽  
Class 1 ◽  
Diseased Fish

ABSTRACTSulfonamide-trimethoprim-resistantAeromonas salmonicidaand motileAeromonasspp. from diseased fish of the GERM-Vet study carried thesul1gene together with mostly cassette-borne trimethoprim resistance genes, including the novel genedfrA28. The sevendfrAanddfrBgenes identified were located mostly in class 1 integrons which commonly harbored other gene cassettes.

Antibiotic Resistance and Characteristics of Integrons in Escherichia coli Isolated from Penaeus vannamei at a Freshwater Shrimp Farm in Zhejiang Province, China

2019 ◽  
Vol 82 (3) ◽  
pp. 470-478 ◽  
Author(s):  
HUI CHENG ◽  
HAN JIANG ◽  
JIEHONG FANG ◽  
CHENG ZHU
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Insertion Sequence ◽  
Zhejiang Province ◽  
Resistant Bacteria ◽  
Penaeus Vannamei ◽  
Shrimp Farm ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1

ABSTRACT Our study was conducted to investigate the antibiotic susceptibility profiles, integrons and their associated gene cassettes (GCs), and insertion sequence common regions of Escherichia coli isolates from Penaeus vannamei collected at a large-scale freshwater shrimp farm in Zhejiang Province, People's Republic of China. A total of 182 E. coli isolates were identified from 200 samples. With the exception of imipenem, isolates were most commonly resistant to β-lactams, followed by tetracylines and sulfonamides. Fifty-two (28.6%) E. coli isolates were classified as multidrug resistant, and the patterns were highly diverse, with 29 types represented. The multiple-antibiotic resistance indices of the isolates were 0.17 to 0.56; 9.3% (17) of the 182 isolates were positive for class 1 integrons, 0.5% (1 isolate) was positive for class 2 integrons, and an insertion sequence common region 1 element was found upstream of the intI1 (integrase) gene in one of the intI1-positive isolates. Four GC arrays were detected in class 1 integrons, and one GC array was detected in class 2 integrons. Although the overall prevalence of antimicrobial-resistant bacteria in P. vannamei was lower than that previously reported for poultry and livestock farms in China, concerns about the inappropriate use of antibiotics and the transmission of antimicrobial-resistant bacteria in aquaculture were raised. Alternative approaches to reducing or replacing the use of antibiotics should be further studied.

Antibiotic resistance patterns of Pseudomonas spp. isolated from faecal wastes in the environment and contaminated surface water

2020 ◽  
Vol 96 (2) ◽  
Author(s):  
Mathilde Camiade ◽  
Josselin Bodilis ◽  
Naouel Chaftar ◽  
Wassila Riah-Anglet ◽  
Johan Gardères ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Enteric Bacteria ◽  
Resistance Pattern ◽  
Pathogenic Species ◽  
Pseudomonas Species ◽  
Class 1 ◽  
Resistance Patterns ◽  
Faecal Bacteria ◽  
Antibiotic Resistance Patterns

ABSTRACT The Pseudomonas genus, which includes environmental and pathogenic species, is known to present antibiotic resistances, and can receive resistance genes from multi-resistant enteric bacteria released into the environment via faecal rejects. This study was aimed to investigate the resistome of Pseudomonas populations that have been in contact with these faecal bacteria. Thus, faecal discharges originating from human or cattle were sampled (from 12 points and two sampling campaigns) and 41 Pseudomonas species identified (316 isolates studied). The resistance phenotype to 25 antibiotics was determined in all isolates, and we propose a specific antibiotic resistance pattern for 14 species (from 2 to 9 resistances). None showed resistance to aminoglycosides, tetracycline, or polymyxins. Four species carried a very low number of resistances, with none to β-lactams. Interestingly, we observed the absence of the transcriptional activator soxR gene in these four species. No plasmid transfer was highlighted by conjugation assays, and a few class 1 but no class 2 integrons were detected in strains that may have received resistance genes from Enterobacteria. These results imply that the contribution of the Pseudomonas genus to the resistome of an ecosystem first depends on the structure of the Pseudomonas populations, as they may have very different resistance profiles.

Assessment appraisal, disposal and transfer of neglected rangers’ diaries created from 1926 to 1930: case of the Kruger National Parks of South Africa

2019 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Nkholedzeni Sidney Netshakhuma
Keyword(s):  
South Africa ◽  
South African ◽  
National Parks ◽  
National Park ◽  
Qualitative Methodology ◽  
Historical Record ◽  
Content Type ◽  
National Archives ◽  
History Of ◽  
Parks Management

Purpose This paper aims to assess the appraisal, disposal and transfer of records of the Kruger National Park (KNP) rangers’ diaries processes from 1926 to 1930 with a view to recommend best practices. Design/methodology/approach The paper applied a qualitative methodology through document analysis, interviews and observations as data collection instruments to analyse contents of rangers’ diaries. The population of the study comprised rangers, a records manager and a representative from the National Archives of South Africa (NARSSA), a member of South Africa National Parks’ management and a scientist from South African National Biodiversity Institute. Findings The key findings revealed that rangers’ diaries contain historical, scientific and cultural information. However, such information is not disseminated to society. Lack of systematic appraisal, arrangement of records led to a loss of institutional memories. The role of National Archives and Records Service of South Africa is not visible to provide guidelines on the preservation of rangers’ records. Research limitations/implications This research is limited to KNP ranger’s diaries created from 1926 to 1930 because it is the period of establishment of the national park and introduction of rangers’ programme. Practical implications The findings are expected to be instrumental towards the preservation of rangers’ diaries within the park. Rangers’ diaries are also potentially of great biogeographical value in constituting a historical record of the plants and animals in a given area, their distribution and population changes, and the effects of human interventions such as game fences and artificially created waterholes, game culling and tourism, not to forget climate change. Social implications Preservation of ranger diaries may lead to documentation of records with historical, scientific and social value. Rangers’ diaries also form part of the national archival heritage of South Africa, as they bridge the gap of undocumented history of the rangers and national parks in South Africa. Originality/value This paper appears to be the first to research the assessment of the appraisal, disposal and transfer of rangers’ diaries created from 1926 to 1930.

scholarly journals The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance

2008 ◽  
Vol 190 (14) ◽  
pp. 5095-5100 ◽  
Author(s):  
Michael Gillings ◽  
Yan Boucher ◽  
Maurizio Labbate ◽  
Andrew Holmes ◽  
Samyuktha Krishnan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Lateral Transfer ◽  
Natural Environments ◽  
Class 1 Integrons ◽  
Wide Range ◽  
Human Food Chain ◽  
Class 1 ◽  
Phylogenetic Signature

ABSTRACT Class 1 integrons are central players in the worldwide problem of antibiotic resistance, because they can capture and express diverse resistance genes. In addition, they are often embedded in promiscuous plasmids and transposons, facilitating their lateral transfer into a wide range of pathogens. Understanding the origin of these elements is important for the practical control of antibiotic resistance and for exploring how lateral gene transfer can seriously impact on, and be impacted by, human activities. We now show that class 1 integrons can be found on the chromosomes of nonpathogenic soil and freshwater Betaproteobacteria. Here they exhibit structural and sequence diversity, an absence of antibiotic resistance genes, and a phylogenetic signature of lateral transfer. Some examples are almost identical to the core of the class 1 integrons now found in pathogens, leading us to conclude that environmental Betaproteobacteria were the original source of these genetic elements. Because these elements appear to be readily mobilized, their lateral transfer into human commensals and pathogens was inevitable, especially given that Betaproteobacteria carrying class 1 integrons are common in natural environments that intersect with the human food chain. The strong selection pressure imposed by the human use of antimicrobial compounds then ensured their fixation and global spread into new species.

scholarly journals Characteristics of Carbapenemase-Producing Enterobacteriaceae in Wastewater Revealed by Genomic Analysis

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Ryota Gomi ◽  
Tomonari Matsuda ◽  
Masaki Yamamoto ◽  
Pei-Hsin Chou ◽  
Michio Tanaka ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Genomic Analysis ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Whole Genome Analysis ◽  
Content Type ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Encoding Genes

ABSTRACT Wastewater is considered a major source of antibiotic-resistant bacteria released into the environment. Here, we characterized carbapenemase-producing Enterobacteriaceae (CPE) in wastewater by whole-genome analysis. Wastewater samples ( n = 40) were collected from municipal wastewater treatment plants and hospital wastewater in Japan and Taiwan. Samples were screened for CPE using selective media, and the obtained isolates were sequenced using an Illumina MiSeq. The isolates ( n = 45) included the following microorganisms: Klebsiella quasipneumoniae ( n = 12), Escherichia coli ( n = 10), Enterobacter cloacae complex ( n = 10), Klebsiella pneumoniae ( n = 8), Klebsiella variicola ( n = 2), Raoultella ornithinolytica ( n = 1), Citrobacter freundii ( n = 1), and Citrobacter amalonaticus ( n = 1). Among the 45 isolates, 38 harbored at least one carbapenemase-encoding gene. Of these, the bla GES ( bla GES-5 , bla GES-6 , and bla GES-24 ) genes were found in 29 isolates. The genes were situated in novel class 1 integrons, but the integron structures were different between the Japanese (In1439 with bla GES-24 and In1440 with bla GES-5 ) and Taiwanese (In1441 with bla GES-5 and In1442 with bla GES-6 ) isolates. Other carbapenemase-encoding genes ( bla VIM-1 , bla NDM-5 , bla IMP-8 , bla IMP-19 , and bla KPC-2 ) were found in one to three isolates. Notably, class 1 integrons previously reported among clinical isolates obtained in the same regions as the present study, namely, In477 with bla IMP-19 and In73 with bla IMP-8 , were found among the Japanese and Taiwanese isolates, respectively. The results indicate that CPE with various carbapenemase-encoding genes in different genetic contexts were present in biologically treated wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.

Sign in / Sign up

Export Citation Format

Share Document