scholarly journals Bacillus subtilis Strain Engineered for Treatment of Soil-Transmitted Helminth Diseases

2013 ◽  
Vol 79 (18) ◽  
pp. 5527-5532 ◽  
Author(s):  
Yan Hu ◽  
Melanie M. Miller ◽  
Alan I. Derman ◽  
Brian L. Ellis ◽  
Rose Gomes Monnerat ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Strain ◽  
Subtilis Strain ◽  
Human Consumption ◽  
Bacterial Strains ◽  
Content Type ◽  
Live Bacteria ◽  
Therapeutic Properties ◽  
Adverse Environmental Conditions ◽  
Hookworm Infections

ABSTRACTSoil-transmitted helminths (hookworms, whipworms, and large roundworms) are agents of intestinal roundworm diseases of poverty that infect upwards of 2 billion people worldwide. A great challenge in treating these diseases is the development of anthelmintic therapeutics that are inexpensive, can be produced in great quantity, and are capable of delivery under varied and adverse environmental conditions. A potential solution to this challenge is the use of live bacteria that are acceptable for human consumption, e.g.,Bacillus subtilis, and that can be engineered with therapeutic properties. In this study, we expressed theBacillus thuringiensisanthelmintic protein Cry5B in a bacterial strain that has been used as a model for live bacterial therapy,Bacillus subtilisPY79. PY79 transformed with a Cry5B expression plasmid (PY79-Cry5B) is able to express Cry5B from the endogenousB. thuringiensis cry5Bpromoter. During sporulation of PY79-Cry5B, Cry5B is packaged as a crystal. Furthermore, Cry5B produced in PY79 is bioactive, with a 50% lethal concentration (LC50) of 4.3 μg/ml against the roundwormCaenorhabditis elegans. PY79-Cry5B was a significantly effective therapeutic in experimentalAncylostoma ceylanicumhookworm infections of hamsters. A single 10-mg/kg (0.071 μmol/kg of body weight) dose of Cry5B administered as a Cry5B-PY79 spore crystal lysate achieved a 93% reduction in hookworm burdens, which is superior on a molar level to reductions seen with clinically used anthelmintics. Given that a bacterial strain such as this one can be produced cheaply in massive quantities, our results demonstrate that the engineering and delivery of live bacterial strains have great potential to treat a significant contributor to poverty worldwide, namely, hookworm disease and other soil-transmitted helminthiasis.

scholarly journals Intracellular serine proteinase of Bacillus subtilis strain Marburg 168. Comparison with the homologous enzyme from Bacillus subtilis strain A-50

1979 ◽  
Vol 179 (2) ◽  
pp. 333-339 ◽  
Author(s):  
A Y Strongin ◽  
D I Gorodetsky ◽  
I A Kuznetsova ◽  
V V Yanonis ◽  
Z T Abramov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Terminal Sequence ◽  
Gramicidin S ◽  
Intracellular Enzymes ◽  
Sepharose 4B ◽  
Homologous Enzyme ◽  
Strict Selection

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

scholarly journals Complete Genome Sequence of Bacillus subtilis Strain WB800N, an Extracellular Protease-Deficient Derivative of Strain 168

2018 ◽  
Vol 7 (18) ◽  
Author(s):  
Haeyoung Jeong ◽  
Da-Eun Jeong ◽  
Seung-Hwan Park ◽  
Seong Joo Kim ◽  
Soo-Keun Choi
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genetically Engineered ◽  
Subtilis Strain ◽  
Secretory Proteins ◽  
Extracellular Proteases ◽  
Content Type ◽  
Protease Deficient

Bacillus subtilis WB800N is a genetically engineered variant of B. subtilis 168, such that all extracellular proteases are disrupted, which enables WB800N to be widely used for the expression of secretory proteins. Here, we report the 4.2-Mb complete genome sequence of WB800N and present all of the disrupted gene structure.

scholarly journals Complete Genome Sequence of Bacillus subtilis Strain DKU_NT_03, Isolated from a Traditional Korean Food Using Soybean (Chung-gook-jang) for High-Quality Nattokinase Activity

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Hee-Won Jeong ◽  
Man-Seok Bang ◽  
Yea-jin Lee ◽  
Su Ji Lee ◽  
Sang-Cheol Lee ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genetic Factors ◽  
Subtilis Strain ◽  
High Quality ◽  
Content Type ◽  
Korean Food

ABSTRACT We present here the complete genome sequence of Bacillus subtilis strain DKU_NT_03 isolated from the traditional Korean food chung-gook-jang, which is made from soybeans. This strain was chosen to identify genetic factors with high-quality nattokinase activity.

scholarly journals Complete Genome Sequence of Undomesticated Bacillus subtilis Strain NCIB 3610

2017 ◽  
Vol 5 (20) ◽  
Author(s):  
Taylor M. Nye ◽  
Jeremy W. Schroeder ◽  
Daniel B. Kearns ◽  
Lyle A. Simmons
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Experimental System ◽  
Subtilis Strain ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Content Type

ABSTRACT Bacillus subtilis is a Gram-positive bacterium that serves as an important experimental system. B. subtilis NCIB 3610 is an undomesticated strain that exhibits phenotypes lost from the more common domesticated laboratory strains. Here, we announce the complete genome sequence of DK1042, a genetically competent derivative of NCIB 3610.

scholarly journals The RapP-PhrP Quorum-Sensing System of Bacillus subtilis Strain NCIB3610 Affects Biofilm Formation through Multiple Targets, Due to an Atypical Signal-Insensitive Allele of RapP

2014 ◽  
Vol 197 (3) ◽  
pp. 592-602 ◽  
Author(s):  
Shira Omer Bendori ◽  
Shaul Pollak ◽  
Dorit Hizi ◽  
Avigdor Eldar
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Response Regulator ◽  
Subtilis Strain ◽  
Transcriptional Level ◽  
Response Regulators ◽  
Multiple Targets ◽  
Content Type ◽  
Negative Effect

The genome ofBacillus subtilis168 encodes eightrap-phrquorum-sensing pairs. Rap proteins of all characterized Rap-Phr pairs inhibit the function of one or several important response regulators: ComA, Spo0F, or DegU. This inhibition is relieved upon binding of the peptide encoded by the cognatephrgene.Bacillus subtilisstrain NCIB3610, the biofilm-proficient ancestor of strain 168, encodes, in addition, therapP-phrPpair on the plasmid pBS32. RapP was shown to dephosphorylate Spo0F and to regulate biofilm formation, but unlike other Rap-Phr pairs, RapP does not interact with PhrP. In this work we extend the analysis of the RapP pathway by reexamining its transcriptional regulation, its effect on downstream targets, and its interaction with PhrP. At the transcriptional level, we show thatrapPandphrPregulation is similar to that of otherrap-phrpairs. We further find that RapP has an Spo0F-independent negative effect on biofilm-related genes, which is mediated by the response regulator ComA. Finally, we find that the insensitivity of RapP to PhrP is due to a substitution of a highly conserved residue in the peptide binding domain of therapPallele of strain NCIB3610. Reversing this substitution to the consensus amino acid restores the PhrP dependence of RapP activity and eliminates the effects of therapP-phrPlocus on ComA activity and biofilm formation. Taken together, our results suggest that RapP strongly represses biofilm formation through multiple targets and that PhrP does not counteract RapP due to a rare mutation inrapP.

scholarly journals Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

2020 ◽  
Vol 17 (22) ◽  
pp. 8446
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Mariadhas Valan Arasu
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Crude Oil ◽  
Solid State Fermentation ◽  
Contaminated Soils ◽  
Quadratic Model ◽  
Subtilis Strain ◽  
Natural Environments ◽  
Bacterial Strains ◽  
Enhanced Production

Crude oil and its derivatives are the most important pollutants in natural environments. Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in recent years. In this study, biosurfactant-producing Bacillus subtilis strain Al-Dhabi-130 was isolated from the marine soil sediment. This organism was cultured in solid-state fermentation using agro-residues to produce cost-effective biosurfactants for the bioremediation of crude-oil contaminated environments. Date molasses improved biosurfactant production and were used for further optimization studies. The traditional “one-variable-at-a-time approach”, “two-level full factorial designs”, and a response surface methodology were used to optimize the concentrations of date molasses and nutrient supplements for surfactant production. The optimum bioprocess conditions were 79.3% (v/w) moisture, 34 h incubation period, and 8.3% (v/v) glucose in date molasses. To validate the quadratic model, the production of biosurfactant was performed in triplicate experiments, with yields of 74 mg/g substrate. These findings support the applications of date molasses for the production of biosurfactants by B. subtilis strain Al-Dhabi-130. Analytical experiments revealed that the bacterial strain degraded various aromatic hydrocarbons and n-alkanes within two weeks of culture with 1% crude oil. The crude biosurfactant produced by the B. subtilis strain Al-Dhabi-130 desorbed 89% of applied crude oil from the soil sample. To conclude, biosurfactant-producing bacterial strains can increase emulsification of crude oil and support the degradation of crude oil.

40 % aq. HF catalyzed three component Synthesis of novel indeno[1,2-b][1,8]naphthyridin-6(11H)-one derivatives and their antimicrobial activity

2020 ◽  
Vol 17 ◽  
Author(s):  
Srinu Bhoomandla ◽  
Phani Raja Kanuparthy ◽  
Rambabu Gundla ◽  
Ramana Reddy Bobbala
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Good Yield ◽  
Diffusion Method ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Aryl Aldehyde

: A Three component Synthesis of novel 5-phenyl-2-(thiophen-2-yl)-4-(trifluoromethyl)-5H-indeno [1,2-b] [1,8] naphthyridin-6(11H)-one derivatives (4a-n) were prepared using 6-phenyl/(thiophen-2-yl)-4-(trifluoromethyl)pyridin-2-amine, 1H-indene-1,3(2H)-dione and aryl aldehyde using 40% aq. HF with good yield. All the synthesized compounds were screened against Gram-positive and Gram-negative bacterial strains and different Candida strains by well diffusion method. Compounds 4c, 4f and 4g showed promising activity on Bacillus subtilis strain and compounds 4c and 4g showed promising activity towards Candida albicans starains.

scholarly journals Complete Genome Sequence of Bacillus subtilis Strain DKU_NT_02, Isolated from Traditional Korean Food Using Soybean (Chung-gook-jang) for High-Quality Poly-γ-Glutamic Acid Activity

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Man-Seok Bang ◽  
Hee-Won Jeong ◽  
Yea-jin Lee ◽  
Su Ji Lee ◽  
Sang-Cheol Lee ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Glutamic Acid ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genetic Factors ◽  
Subtilis Strain ◽  
High Quality ◽  
Content Type ◽  
Korean Food

The complete genome sequence of Bacillus subtilis strain DKU_NT_02, isolated from traditional Korean food using soybeans (chung-gook-jang), is presented here. This strain was chosen to help identify genetic factors with high-quality poly-γ-glutamic acid (γPGA) activity.

scholarly journals Complete Genome Sequence of Bacillus subtilis Strain MH1, Which Has a High Level of Bacteriocin-Like Activity, Isolated from Soil in Bangladesh

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Mohammad Shahnoor Hossain ◽  
Marufa Zerin Akhter ◽  
Muhammad Maqsud Hossain ◽  
M. Asaduzzaman Shishir ◽  
Shakila Nargis Khan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Messenger Rna ◽  
Pathogenic Bacteria ◽  
Gc Content ◽  
Full Genome Sequence ◽  
Subtilis Strain ◽  
Bacteriocin Activity ◽  
Content Type ◽  
High Level

ABSTRACT Bacillus subtilis MH1 demonstrates a high level of bacteriocin activity against several pathogenic bacteria. We announce here the full-genome sequence of strain MH1, isolated from soil in Bangladesh. This genome length is 4,094,053 bp, with 43.5% GC content, 4,217 coding sequences (CDS), 10 rRNA, 84 tRNA, and 1 transfer-messenger RNA (tmRNA).

scholarly journals Complete Genome Sequence of Bacillus subtilis Strain 29R7-12, a Piezophilic Bacterium Isolated from Coal-Bearing Sediment 2.4 Kilometers below the Seafloor

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Yuli Wei ◽  
Junwei Cao ◽  
Jiasong Fang ◽  
Chiaki Kato ◽  
Weicheng Cui
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Subtilis Strain ◽  
Northwestern Pacific ◽  
Valuable Resource ◽  
Deep Biosphere ◽  
Content Type ◽  
Subsurface Environments

ABSTRACT Here, we report the genome sequence of Bacillus subtilis strain 29R7-12, a piezophilic bacterium isolated from coal-bearing sediment down to ~2.4 km below the ocean floor in the northwestern Pacific. The strain is a Gram-positive spore-forming bacterium, closely related to Bacillus subtilis within the phylum Firmicutes. This is the first complete genome sequence of a Bacillus subtilis strain from the deep biosphere. The genome sequence will provide a valuable resource for comparative studies of microorganisms from the surface and subsurface environments.

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