scholarly journals Fecal Microbiota Transplant from Highly Feed-Efficient Donors Shows Little Effect on Age-Related Changes in Feed-Efficiency-Associated Fecal Microbiota from Chickens

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Sina-Catherine Siegerstetter ◽  
Renée M. Petri ◽  
Elizabeth Magowan ◽  
Peadar G. Lawlor ◽  
Qendrim Zebeli ◽  
...  
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Nutrient Retention ◽  
Fecal Microbiota ◽  
Microbial Colonization ◽  
Microbiota Composition ◽  
Fecal Microbiota Transplant ◽  
Content Type ◽  
Age Related

ABSTRACT Chickens with good or poor feed efficiency (FE) have been shown to differ in their intestinal microbiota composition. This study investigated differences in the fecal bacterial community of highly and poorly feed-efficient chickens at 16 and 29 days posthatch (dph) and evaluated whether a fecal microbiota transplant (FMT) from feed-efficient donors early in life can affect the fecal microbiota in chickens at 16 and 29 dph and chicken FE and nutrient retention at 4 weeks of age. A total of 110 chickens were inoculated with a FMT or a control transplant (CT) on dph 1, 6, and 9 and ranked according to residual feed intake (RFI; the metric for FE) on 30 dph. Fifty-six chickens across both inoculation groups were selected as the extremes in RFI (29 low, 27 high). RFI-related fecal bacterial profiles were discernible at 16 and 29 dph. In particular, Lactobacillus salivarius, Lactobacillus crispatus, and Anaerobacterium operational taxonomic units were associated with low RFI (good FE). Multiple administrations of the FMT only slightly changed the fecal bacterial composition, which was supported by weighted UniFrac analysis, showing similar bacterial communities in the feces of both inoculation groups at 16 and 29 dph. Moreover, the FMT did not change the RFI and nutrient retention of highly and poorly feed-efficient recipients, whereas it tended to increase feed intake and body weight gain in female chickens. This finding suggests that host- and environment-related factors may more strongly affect chicken fecal microbiota and FE than the FMT. IMPORTANCE Modulating the chicken's early microbial colonization using a FMT from highly feed-efficient donor chickens may be a promising tool to establish a more desirable bacterial profile in recipient chickens, thereby improving host FE. Although FE-associated fecal bacterial profiles at 16 and 29 dph could be established, the microbiota composition of a FMT, when administered early in life, may not be a strong factor modulating the fecal microbiota at 2 to 4 weeks of life and reducing the variation in chicken's FE. Nevertheless, the present FMT may have potential benefits for growth performance in female chickens.

scholarly journals Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

2017 ◽  
Vol 83 (15) ◽  
Author(s):  
Ursula M. McCormack ◽  
Tânia Curião ◽  
Stefan G. Buzoianu ◽  
Maria L. Prieto ◽  
Tomas Ryan ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Intestinal Microbiota ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Residual Feed Intake ◽  
Production Costs ◽  
Microbiota Composition ◽  
Content Type ◽  
Intestinal Microbes ◽  
Pig Production

ABSTRACT Feed efficiency (FE) is critical in pig production for both economic and environmental reasons. As the intestinal microbiota plays an important role in energy harvest, it is likely to influence FE. Therefore, our aim was to characterize the intestinal microbiota of pigs ranked as low, medium, and high residual feed intake ([RFI] a metric for FE), where genetic, nutritional, and management effects were minimized, to explore a possible link between the intestinal microbiota and FE. Eighty-one pigs were ranked according to RFI between weaning and day 126 postweaning, and 32 were selected as the extremes in RFI (12 low, 10 medium, and 10 high). Intestinal microbiota diversity, composition, and predicted functionality were assessed by 16S rRNA gene sequencing. Although no differences in microbial diversity were found, some RFI-associated compositional differences were revealed, principally among members of Firmicutes, predominantly in feces at slaughter (albeit mainly for low-abundance taxa). In particular, microbes associated with a leaner and healthier host (e.g., Christensenellaceae, Oscillibacter, and Cellulosilyticum) were enriched in low RFI (more feed-efficient) pigs. Differences were also observed in the ileum of low RFI pigs; most notably, Nocardiaceae (Rhodococcus) were less abundant. Predictive functional analysis suggested improved metabolic capabilities in these animals, especially within the ileal microbiota. Higher ileal isobutyric acid concentrations were also found in low RFI pigs. Overall, the differences observed within the intestinal microbiota of low RFI pigs compared with that of their high RFI counterparts, albeit relatively subtle, suggest a possible link between the intestinal microbiota and FE in pigs. IMPORTANCE This study is one of the first to show that differences in intestinal microbiota composition, albeit subtle, may partly explain improved feed efficiency (FE) in low residual feed intake (RFI) pigs. One of the main findings is that, although microbial diversity did not differ among animals of varying FE, specific intestinal microbes could potentially be linked with porcine FE. However, as the factors impacting FE are still not fully understood, intestinal microbiota composition may not be a major factor determining differences in FE. Nonetheless, this work has provided a potential set of microbial biomarkers for FE in pigs. Although culturability could be a limiting factor and intervention studies are required, these taxa could potentially be targeted in the future to manipulate the intestinal microbiome so as to improve FE in pigs. If successful, this has the potential to reduce both production costs and the environmental impact of pig production.

scholarly journals Feed Restriction Reveals Distinct Serum Metabolome Profiles in Chickens Divergent in Feed Efficiency Traits

Metabolites ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 38 ◽  
Author(s):  
Barbara Metzler-Zebeli ◽  
Sina-Catherine Siegerstetter ◽  
Elizabeth Magowan ◽  
Peadar Lawlor ◽  
Niamh O’Connell ◽  
...  
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Energy Demand ◽  
Body Weight Gain ◽  
Serum Glucose ◽  
Arachidonic Acid Metabolism ◽  
Feed Restriction ◽  
Physiological Factors ◽  
Ad Libitum ◽  
Almost All

Restrictive feeding influences systemic metabolism of nutrients; however, this impact has not been evaluated in chickens of diverging feed efficiency. This study investigated the effect of ad libitum versus restrictive feeding (85% of ad libitum) on the serum metabolome and white blood cell composition in chickens of diverging residual feed intake (RFI; metric for feed efficiency). Blood samples were collected between days 33 and 37 post-hatch. While serum glucose was similar, serum uric acid and cholesterol were indicative of the nutritional status and chicken’s RFI, respectively. Feed restriction and RFI rank caused distinct serum metabolome profiles, whereby restrictive feeding also increased the blood lymphocyte proportion. Most importantly, 10 amino acids were associated with RFI rank in birds, whereas restrictive feeding affected almost all detected lysophosphatidylcholines, with 3 being higher and 6 being lower in restrictively compared to ad libitum fed chickens. As indicated by relevance networking, isoleucine, lysine, valine, histidine, and ornithine were the most discriminant for high RFI, whereas 3 biogenic amines (carnosine, putrescine, and spermidine) and 3 diacyl-glycerophospholipids (38:4, 38:5, and 40:5) positively correlated with feed intake and body weight gain, respectively. Only for taurine, feed intake mostly explained the RFI-associated variation, whereas for most metabolites, other host physiological factors played a greater role for the RFI-associated differences, and was potentially related to insulin-signaling, phospholipase A2, and arachidonic acid metabolism. Alterations in the hepatic synthesis of long-chain fatty acids and the need for precursors for gluconeogenesis due to varying energy demand may explain the marked differences in serum metabolite profiles in ad libitum and restrictively fed birds.

scholarly journals Feed Efficiency Can Be Sustained in Pigs Fed with Locally Produced Narbon Vetch (Vicia narbonensis L.)

2020 ◽  
Vol 12 (10) ◽  
pp. 3993
Author(s):  
Emilio Gómez Izquierdo ◽  
Luis Gomez-Raya ◽  
Eduardo de Mercado de la Peña ◽  
Juan José Ciruelos ◽  
Wendy M. Rauw
Keyword(s):  
Soybean Meal ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Soil Conditions ◽  
Feed Conversion ◽  
Economic Implications ◽  
Livestock Feeds ◽  
Daily Body Weight ◽  
Feed Costs

There is an interest in replacing soybean meal with locally produced ingredients in livestock feeds. Narbon vetch is resistant to unfavorable climatic and soil conditions, common pests, and has a favorable nutritional profile. The effect of substitution of soybean meal with 0% (V0), 5% (V5), 10% (V10), and 20% (V20) inclusion of Narbon vetch on growth curve parameters, daily body weight gain (DBWG), daily feed intake (DFI), feed conversion efficiency (FCE), and residual feed intake (RFI) was investigated in 47 Duroc × Iberian barrows in 16 periods (a total of 125 d). DFI and DBWG were reduced (p < 0.05) up to four weeks after introduction of the novel feed in V20 and V10. Small, mostly nonsignificant differences existed between treatments in FCE and RFI. However, because of accumulative small differences in feed efficiency between the four diets, pigs in V0 reached the highest BW, and pigs in V20 reached the lowest BW on a similar feed intake. Economic implications of Narbon vetch inclusion depend on the extra amount of feed required and associated feed costs, and on the costs of additional days on-farm required to reach a given slaughter weight.

Effect of Replacement of Antibiotic with Probiotic on Performance, Carcass Characteristics and Nutrient Retention in Broilers Fed with Meat Cum Bone Meal

2020 ◽  
Author(s):  
G. Madhuri ◽  
B. Swathi ◽  
P. Radhakrishna ◽  
D. Nagalakshmi
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Crude Protein ◽  
Body Weight Gain ◽  
Carcass Characteristics ◽  
Nutrient Retention ◽  
Basal Diet ◽  
Feed Conversion ◽  
Bone Meal

The effect of replacing an antibiotic growth promoter in the diet with a probiotic on growth performance, carcass characteristics and nutrient retention was studied in broilers (Vencobb 430) fed with and without meat cum bone meal (MBM). This experiment which lasted for 42 days consisted of six dietary treatments viz., basal diet (BD), basal diet with MBM (MBM), basal diet with antibiotic (AB), basal diet with probiotic (PB), basal diet with MBM and antibiotic (MBM+AB) and basal diet with MBM and probiotic (MBM+PB). Three-hundred, day-old chicks were randomly allotted in the beginning to these six groups. Body weight gain, feed intake and feed conversion ratio were significantly (P less than 0.05) affected at 4th week of age. Overall, highest body weight gain was recorded in birds fed with AB and PB. Inclusion of probiotic in MBM also showed improved body weight gain when compared to MBM group. Highest feed intake and feed conversion ratio was recorded with AB and PB groups. Birds supplemented with MBM+PB showed improved feed intake and feed efficiency when compared to MBM+AB and MBM diet supplemented groups. Higher dry matter, crude protein, calcium and phosphorus retention (P less than 0.05) was observed in birds supplemented with PB or AB in diet without MBM when compared to their control. In MBM supplemented groups addition of PB or AB improved crude protein retention. Birds supplemented with PB or AB with or without MBM showed a significant (P less than 0.05) increase in thigh yield and a non significant reduction in fat yield when compared to their control groups.

scholarly journals Altered Fecal Microbiota Composition Associated with Food Allergy in Infants

2014 ◽  
Vol 80 (8) ◽  
pp. 2546-2554 ◽  
Author(s):  
Zongxin Ling ◽  
Zailing Li ◽  
Xia Liu ◽  
Yiwen Cheng ◽  
Yueqiu Luo ◽  
...  
Keyword(s):  
Food Allergy ◽  
Intestinal Microbiota ◽  
Fecal Microbiota ◽  
Sensu Stricto ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Human Beings ◽  
Actual Structure ◽  
Content Type ◽  
Ige Mediated

ABSTRACTIncreasing evidence suggests that perturbations in the intestinal microbiota composition of infants are implicated in the pathogenesis of food allergy (FA), while the actual structure and composition of the intestinal microbiota in human beings with FA remain unclear. Microbial diversity and composition were analyzed with parallel barcoded 454 pyrosequencing targeting the 16S rRNA gene hypervariable V1-V3 regions in the feces of 34 infants with FA (17 IgE mediated and 17 non-IgE mediated) and 45 healthy controls. Here, we showed that several key FA-associated bacterial phylotypes, but not the overall microbiota diversity, significantly changed in infancy fecal microbiota with FA and were associated with the development of FA. The proportion of abundantBacteroidetes,Proteobacteria, andActinobacteriaphyla were significantly reduced, while theFirmicutesphylum was highly enriched in the FA group (P< 0.05). AbundantClostridiaceae1 organisms were prevalent in infants with FA at the family level (P= 0.016). FA-enriched phylotypes negatively correlated with interleukin-10, for example, the generaEnterococcusandStaphylococcus. Despite profound interindividual variability, levels of 20 predominant genera were significantly different between the FA and healthy control groups (P< 0.05). Infants with IgE-mediated FA had increased levels ofClostridium sensu strictoandAnaerobacterand decreased levels ofBacteroidesandClostridiumXVIII (P< 0.05). A positive correlation was observed betweenClostridium sensu strictoand serum-specific IgE (R= 0.655,P< 0.001). The specific microbiota signature could distinguish infants with IgE-mediated FA from non-IgE-mediated ones. Detailed microbiota analysis of a well-characterized cohort of infants with FA showed that dysbiosis of fecal microbiota with several FA-associated key phylotypes may play a pathogenic role in FA.

scholarly journals Susceptibility to Campylobacter Infection Is Associated with the Species Composition of the Human Fecal Microbiota

mBio ◽  
2014 ◽  
Vol 5 (5) ◽  
Author(s):  
Johan Dicksved ◽  
Patrik Ellström ◽  
Lars Engstrand ◽  
Hilpi Rautelin
Keyword(s):  
Gut Microbiota ◽  
Mouse Models ◽  
Fecal Microbiota ◽  
Microbiota Composition ◽  
Content Type ◽  
Culture Positive ◽  
Abattoir Workers ◽  
Gut Microbiota Composition

ABSTRACTThe gut microbiota is essential for human health, but very little is known about how the composition of this ecosystem can influence and respond to bacterial infections. Here we address this by prospectively studying the gut microbiota composition before, during, and after naturalCampylobacterinfection in exposed poultry abattoir workers. The gut microbiota composition was analyzed with 16S amplicon sequencing of fecal samples from poultry abattoir workers during the peak season ofCampylobacterinfection in Sweden. The gut microbiota compositions were compared between individuals who became culture positive forCampylobacterand those who remained negative. Individuals who becameCampylobacterpositive had a significantly higher abundance ofBacteroides(P= 0.007) andEscherichia(P= 0.002) species than those who remained culture negative. Furthermore, this group had a significantly higher abundance ofPhascolarctobacterium(P= 0.017) andStreptococcus(P= 0.034) sequences than theCampylobacter-negative group, which had an overrepresentation ofClostridiales(P= 0.017), unclassifiedLachnospiraceae(P= 0.008), andAnaerovorax(P= 0.015) sequences. Intraindividual comparisons of the fecal microbiota compositions yielded small differences over time inCampylobacter-negative participants, but significant long-term changes were found in theCampylobacter-positive group (P< 0.005). The results suggest that the abundance of specific genera in the microbiota reduces resistance toCampylobactercolonization in humans and thatCampylobacterinfection can have long-term effects on the composition of the human fecal microbiota.IMPORTANCEStudies using mouse models have made important contributions to our understanding of the role of the gut microbiota in resistance to bacterial enteropathogen colonization. The relative abundances ofEscherichia coliandBacteroidesspecies have been pointed out as important determinants of susceptibility to Gram-negative pathogens in general andCampylobacterinfection in particular. In this study, we assessed the role of the human gut microbiota in resistance toCampylobactercolonization by studying abattoir workers that are heavily exposed to these bacteria. Individuals with a certain composition of the gut microbiota became culture positive forCampylobacter. As their microbiotas were characterized by high abundances ofBacteroidesspp. andE. coli, well in line with the findings with mouse models, these bacterial species likely play an important role in colonization resistance also in humans.

scholarly journals Age-related Aortic Stiffness Can Be Transferred and Ameliorated via Fecal Microbiota Transplant in Mice

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. 829-830
Author(s):  
Nathan Greenberg ◽  
Nicholas VanDongen ◽  
Rachel Gioscia-Ryan ◽  
Abigail Casso ◽  
David Hutton ◽  
...  
Keyword(s):  
Aortic Stiffness ◽  
Structural Changes ◽  
Fecal Microbiota ◽  
Structural Proteins ◽  
Mechanical Stiffness ◽  
Fecal Microbiota Transplant ◽  
Age Related ◽  
Aortic Stiffening ◽  
Young Mice

Abstract Age-related increases in aortic stiffness contribute to the development of cardiovascular diseases (CVD). To determine whether the gut microbiome (GM) modulates age-related aortic stiffening, we performed fecal microbiota transplants (FMT) between young (Y; 3 month) and older (O; 25 month) male C57BL/6N mice. Following antibiotic treatment (to suppress endogenous microbiota), mice received weekly FMT (fecal samples collected at baseline) via oral gavage for 8-16 weeks from their own (i.e., sham condition: Y-y, O-o [RECIPIENT-donor]) or opposite age group (Y-o, O-y) (N=8-12/group). In vivo aortic stiffness (pulse wave velocity [PWV]) was higher in older vs. young mice at baseline (382±8 vs. 328±7cm/sec, mean±SE, P&lt;0.001). Arterial phenotypes were transferred such that old microbiota transplanted into young mice increased, while young into old decreased, PWV (Y-y: 325±10 vs. Y-o: 362±10cm/sec, P=0.022; O-o: 409±10 vs. O-y: 335±6cm/sec, P&lt;0.001). Intrinsic mechanical stiffness of excised aortic rings (elastic modulus) increased after transplant of old into young (Y-y: 2141±223 vs. Y-o: 3218±394kPA, P=0.022), and decreased with young into old (O-O: 3263±217 vs. O-y: 2602±136kPA, P=0.016), indicating the GM mediates aortic stiffening by modulating structural changes in the arterial wall. Age-related increases in aortic abundance of advanced glycation end products (AGEs), which cross-link arterial structural proteins, tended to be transferred by the GM (Y-y: 0.022±0.001 vs. Y-o: 0.038±0.006 A.U., P=0.11; O-o: 0.120±0.029 vs. O-y: 0.038±0.009 A.U., P=0.06). The aging GM can induce aortic stiffening via promoting AGEs accumulation and crosslinking of arterial structural proteins, and thus might be a promising target for preventing/treating age-related aortic stiffening and CVD.

scholarly journals Effect of feed supplementation through turmeric and Tulsi leaves on growth of broiler chicks in deep litter system

2020 ◽  
Vol 15 (2) ◽  
pp. 59-65
Author(s):  
U.K. Shukla ◽  
Deepak Pandey ◽  
Shri Kant
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Research Work ◽  
The Body ◽  
Feed Consumption ◽  
Broiler Chicks ◽  
Feed Supplementation

The experiment was conducted at the livestock production and management unit, faculty of agriculture. MGCGV Chitrakoot – Satna ( M.P.). To complete the research work following steps were followed. Day old 75 broiler chicks (DOC) of same hatch were produced and reared in of Deep litter system. The chicks were weighed, leg banded and distributed randomly into five groups of 15 chicks each as treatment. Chicks of each treatment were further divided into three sub groups of five chicks in each in Randomized Block Design (RBD). Chicks of each sub group were accommodated comfortably in deep letter system providing 1 sq. ft./ chick. Chicks were fed standard starter ration upto 3 weeks age (1 to 21 days) and then broiler finisher ration upto 3-4 weeks (22-28 days). An experiment was conducted with DOC to 75 broilers chicks divided into five groups T0, T1,T2 and T4 which were supplemented with turmeric and Tulsi leaves powder @ 1.0g, 2.0g, 3.0g and 4.0g/kg of broiler ration, respectively. Weekly observations were recorded for live body weight, weekly gain in weight, weekly feed consumption and feed conversion of broiler for four weeks. Based on the results of the experiment, it may be concluded that feed supplementation with turmeric (Curcuma longa) powder influenced the body weight, gain in body weight, feed intake and feed efficiency of broiler chicks. Based on the result of the experiment, it may be concluded that feed supplementation with turmeric + Tulsi leaves powder influenced body weight, gain in body weight, feed intake and feed efficiency of broiler chicks. Based on feed intake and feed efficiency, the best performance of broilers was obtained with feed supplementation of 3g turmeric + 3g Tulsi powder per kg of standard ration (T3), followed by 4g turmeric + 4g Tulsi leaf powder (T4). Treatment T3 was economically superior over all other treatments and control.

scholarly journals Gleaning Insights from Fecal Microbiota Transplantation and Probiotic Studies for the Rational Design of Combination Microbial Therapies

2016 ◽  
Vol 30 (1) ◽  
pp. 191-231 ◽  
Author(s):  
Lauren E. Hudson ◽  
Sarah E. Anderson ◽  
Anita H. Corbett ◽  
Tracey J. Lamb
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Rational Design ◽  
Fecal Microbiota Transplantation ◽  
Gastrointestinal Diseases ◽  
Fecal Microbiota ◽  
Inflammatory Disorder ◽  
Fecal Microbiota Transplant ◽  
Content Type ◽  
Inflammatory Bowel

SUMMARY Beneficial microorganisms hold promise for the treatment of numerous gastrointestinal diseases. The transfer of whole microbiota via fecal transplantation has already been shown to ameliorate the severity of diseases such as Clostridium difficile infection, inflammatory bowel disease, and others. However, the exact mechanisms of fecal microbiota transplant efficacy and the particular strains conferring this benefit are still unclear. Rationally designed combinations of microbial preparations may enable more efficient and effective treatment approaches tailored to particular diseases. Here we use an infectious disease, C. difficile infection, and an inflammatory disorder, the inflammatory bowel disease ulcerative colitis, as examples to facilitate the discussion of how microbial therapy might be rationally designed for specific gastrointestinal diseases. Fecal microbiota transplantation has already shown some efficacy in the treatment of both these disorders; detailed comparisons of studies evaluating commensal and probiotic organisms in the context of these disparate gastrointestinal diseases may shed light on potential protective mechanisms and elucidate how future microbial therapies can be tailored to particular diseases.

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