scholarly journals Site-Specific Profiling of the Dental Mycobiome Reveals Strong Taxonomic Shifts during Progression of Early-Childhood Caries

2020 ◽  
Vol 86 (7) ◽  
Author(s):  
Lauren M. O’Connell ◽  
Ryan Santos ◽  
Garrett Springer ◽  
Robert A. Burne ◽  
Marcelle M. Nascimento ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Disease Progression ◽  
Early Childhood Caries ◽  
Oral Microbiome ◽  
Health Concern ◽  
Holistic View ◽  
Site Specific ◽  
Content Type ◽  
Childhood Caries

ABSTRACT Dental caries is one of the most common diseases worldwide. Bacteria and fungi are both commensals in the oral cavity; however, most research regarding caries has focused on bacterial impacts. The oral fungal mycobiome associated with caries is not well characterized, and its role in disease is unclear. ITS1 amplicon sequencing was used to generate taxonomic profiles from site-specific supragingival plaque samples (n = 82) obtained from 33 children with different caries status. Children were either caries free (CF), caries active with enamel lesions (CAE), or caries active with dentin lesions (CA). Plaque samples were collected from caries-free surfaces (PF) and from enamel (PE) and dentin (PD) lesions. Taxonomic profiles representing the different categorizations (CF-PF, CAE-PF, CAE-PE, CA-PF, CA-PE, and CA-PD) were used to characterize the mycobiome and its change through disease progression. A total of 139 fungal species were identified. Candida albicans was the most abundant species, followed by Candida dubliniensis. We found that severely progressed plaque communities (CA-PD) were significantly different from healthy plaque communities (CF-PF). A total of 32 taxa were differentially abundant across the plaque categories. C. albicans, C. dubliniensis, Nigrospora oryzae, and an unclassified Microdochium sp. were correlated with caries, whereas 12 other taxa were correlated with health. C. dubliniensis increased steadily as caries progressed, suggesting that C. dubliniensis may play an important role in caries pathogenicity. In contrast, four health-associated fungal taxa have the potential to antagonize the cariogen Streptococcus mutans via xylitol production, suggesting a possible fungal mechanism that could contribute to maintenance of dental health. IMPORTANCE Early-childhood caries is one of the most prevalent diseases in children worldwide and, while preventable, remains a global public health concern. Untreated cavities are painful and expensive and can lead to tooth loss and a lower quality of life. Caries are driven by acid production via microbial fermentation of dietary carbohydrates, resulting in enamel erosion. While caries is a well-studied disease, most research has focused on bacterial impacts, even though fungi are commensal organisms living within the plaque biofilm. There is very little known about how fungi impact caries pathogenicity. The elucidation of fungal taxa involved in caries disease progression is necessary for a more holistic view of the human oral microbiome. Data from this study will improve our understanding of how the fungal community changes as disease progresses and provide insight into the complex etiology of dental caries, which is necessary for the development of treatment plans and preventative measures.

scholarly journals Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities

mSystems ◽  
2019 ◽  
Vol 4 (6) ◽  
Author(s):  
Yuan Wang ◽  
Sa Wang ◽  
Chunyan Wu ◽  
Xi Chen ◽  
Zhuhui Duan ◽  
...  
Keyword(s):  
Early Childhood ◽  
Preschool Children ◽  
Dental Caries ◽  
Early Childhood Caries ◽  
Species Level ◽  
Oral Microbiome ◽  
Strain Level ◽  
Oral Disease ◽  
Functional Profile ◽  
Content Type

ABSTRACT Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe early childhood caries (ECC), and 19 age-matched, caries-free children as controls were recruited. Saliva samples were collected from the participants and were subjected to metagenomic analyses, whereby the oral microbial communities were investigated. The metagenomic analyses revealed substantial microbiota differences between the two groups, indicating apparent shifts of the oral microbiome present in the ECC group. At the species level, the ECC-enriched microbes included Prevotella amnii, Shuttleworthia satelles, Olsenella uli, and Anaeroglobus geminatus. Interestingly, Actinomyces odontolyticus and Actinomyces graevenitzii exhibited apparent differences at the strain level but not the species level between the ECC and control groups. Functional examination showed that the ECC group displayed extensive alterations in metabolic genes/pathways/modules, including enriched functions in sugar metabolism. Finally, an SVM (support vector machine) classifier comprising seven species was developed and generated a moderately good performance in predicting caries onset (area under the receiver operating characteristic curve [AUC] = 78.33%). Together, these findings indicate that caries is associated with considerable changes in the oral microbiome, some of which can potentially be exploited as therapeutic targets or diagnostic markers. (This study has been registered at ClinicalTrials.gov under registration no. NCT02341352.) IMPORTANCE Dental caries is a highly prevalent oral disease that can lead to severe dental damage and may greatly compromise the quality of life of the affected individuals. Previous studies, including those based on 16S rRNA gene, have revealed that the oral microbiota plays a prominent role in development of the disease. But the approach of those studies was limited in analyzing several key microbiome traits, including species- or strain-level composition and functional profile. Here, we performed metagenomic analyses for a cohort of preschool children with or without caries. Our results showed that caries was associated with extensive microbiota differences at various taxonomic and functional levels. Some caries-associated species had not been previously reported, some of which may have significant clinical implications. A microbiome gene catalogue from children with caries was constructed for the first time. The results demonstrated that caries is associated with alterations of the oral microbiome, including changes in microbial composition and metabolic functional profile.

Dental Caries Severity and Nutritional Status of Nigerian Preschool Children

2021 ◽  
pp. 238008442110021
Author(s):  
O.O. Olatosi ◽  
A.A. Alade ◽  
T. Naicker ◽  
T. Busch ◽  
A. Oyapero ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Nutritional Status ◽  
Early Childhood Caries ◽  
Z Score ◽  
Cross Sectional ◽  
Risk Of Death ◽  
Malnourished Children ◽  
Z Scores ◽  
Childhood Caries

Introduction: Malnutrition in children is one of the most prevalent global health challenges, and malnourished children have a higher risk of death from childhood diseases. Early childhood caries (ECC) is the most common chronic disease of childhood. Complications from ECC such as pain, loss of tooth/teeth, and infection can undermine a child’s nutrition and growth. Aim: This study aims to evaluate the severity of decay, missing, and filled tooth (dmft) by nutritional status using the z scores of the anthropometric measurements: height for age (HFA), weight for age (WFA), weight for height (WFH), and body mass index for age (BMIA) among children with ECC in Nigeria. Study Design: This is a cross-sectional study conducted in 5 local government areas (LGAs) in Lagos State, Nigeria. A multistage sampling technique was used. Results: A total of 273 cases of ECC were included in the analyses (mean age 4.19 ± 0.96 y). Overall, the mean dmft was 3.04 ± 2.28, and most (96%) were accounted for by untreated decay. The distribution of dmft within the different z score categories of BMIA (<–3 = severely wasted, –2 to –3 = wasted, –2 to +2 = normal, +2 to +3 = overweight and >+3 = obese) showed the highest dmft scores among the combined severely wasted and wasted groups, lowest among children with normal z scores, and intermediate in the overweight and obese groups. There was a significant negative correlation between BMIA z score, WFH z score, and dmft ( r = −0.181, P < 0.05 and r = −0.143, P < 0.05, respectively). However, the correlations between HFA z score, WFA z score, and dmft were positive but not significant ( r = 0.048, P = 0.44 and r = 0.022, P = 0.77, respectively). Conclusion: Our study showed an increased severity of dental caries among severely wasted or wasted children with ECC compared to those of normal or overweight. Knowledge Transfer Statement: The results from this study will raise awareness among clinicians and policy makers on the need for a primary prevention program for early childhood caries in countries with high burden of malnutrition and limited resources. Also, it will help draw the attention of clinicians to the caries status of malnourished children that can be managed to improve the nutritional outcomes.

Streptococcus Mutans Strains in Mother-Child Pairs of Children with Early Childhood Caries

2019 ◽  
Vol 43 (4) ◽  
pp. 252-256 ◽  
Author(s):  
Priya Subramaniam ◽  
Revathy Suresh
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Mutans Streptococci ◽  
Similar Distribution ◽  
Healthy Children ◽  
Only Children ◽  
Polymerase Chain ◽  
Childhood Caries

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

scholarly journals Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Balakrishnan Kalpana ◽  
Puniethaa Prabhu ◽  
Ashaq Hussain Bhat ◽  
Arunsaikiran Senthilkumar ◽  
Raj Pranap Arun ◽  
...  
Keyword(s):  
Early Childhood ◽  
Bacterial Diversity ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Oral Microbiome ◽  
Oral Disease ◽  
Indian Children ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

AbstractDental caries is the most prevalent oral disease affecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidification due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were significantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with significant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of biofilm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound biofilm formation and augmented the growth and enhanced biofilm formation in S. mutans in both dual and multispecies cultures.

scholarly journals Early Childhood Dental Caries, Mouth Pain, and Malnutrition in the Ecuadorian Amazon Region

2017 ◽  
Author(s):  
Marvin So ◽  
Yianni A. Ellenikiotis ◽  
Hannah M. Husby ◽  
Cecilia Leonor Paz ◽  
Brittany Seymour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Young Children ◽  
Nutritional Status ◽  
Early Childhood Caries ◽  
Multivariate Regression Analysis ◽  
Cross Sectional Study ◽  
Nutrition Status ◽  
Cross Sectional ◽  
Childhood Caries

Malnutrition and dental caries in early childhood remain persistent and intertwined global health challenges, particularly for indigenous and geographically-remote populations. To examine the prevalence and associations between early childhood dental caries, parent-reported mouth pain and malnutrition in the Amazonian region of Ecuador, we conducted a cross-sectional study of the oral health and nutrition status of 1,407 children from birth through age 6 in the &ldquo;Alli Kiru&rdquo; program (2011-2013). We used multivariate regression analysis to examine relationships between severe caries, parent-reported mouth pain measures, and nutritional status. The prevalence of dental caries was 65.4%, with 44.7% of children having deep or severe caries, and 33.8% reporting mouth pain. The number of decayed, missing and filled teeth dmft) increased dramatically with age. Malnutrition was prevalent, with 35.9% of children stunted, 1.1% wasted, 7.4% underweight, and 6.8% overweight. As mouth pain increased in frequency, odds for severe caries increased. For each unit increase in mouth pain frequency interfering with sleeping, children had increased odds for underweight (AOR: 1.27; 95% CI: 1.02 &ndash; 1.54) and decreased odds for overweight (AOR: 0.76; 95% CI: 0.58 &ndash; 0.97). This relationship was most pronounced among 3-6 year-olds. Early childhood caries, mouth pain and malnutrition were prevalent in this sample of young children. Parent-reported mouth pain was associated with severe caries, and mouth pain interfering with sleeping was predictive of poor nutritional status. We demonstrate the utility of a parsimonious parent-reported measure of mouth pain to predict young children&rsquo;s risk for severe early childhood caries and malnutrition, which has implications for community health interventions.

scholarly journals Nine years’ trend of dental caries and severe early childhood caries among 3-6-year-old children in Babol, Northern Iran

10.19082/4683 ◽  
2017 ◽  
Vol 9 (6) ◽  
pp. 4683-4688 ◽  
Author(s):  
Mohammad Mehdi Naghibi Sistani ◽  
Zohreh Hataminia ◽  
Mahmoud Hajiahmadi ◽  
Effat Khodadadi
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Early Childhood Caries ◽  
Northern Iran ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

scholarly journals A Formative Assessment of Social Determinants of Health Related to Early Childhood Caries in Two American Indian Communities

2021 ◽  
Vol 18 (18) ◽  
pp. 9838
Author(s):  
Kristan Elwell ◽  
Carolyn Camplain ◽  
Christine Kirby ◽  
Katharine Sanderson ◽  
Gloria Grover ◽  
...  
Keyword(s):  
Early Childhood ◽  
Oral Health ◽  
Dental Caries ◽  
Formative Assessment ◽  
American Indian ◽  
Social Determinants Of Health ◽  
Social Determinants ◽  
Early Childhood Caries ◽  
The Social ◽  
Childhood Caries

In the United States, children from diverse ethnic groups and those with low socioeconomic status are at a significantly increased risk for early childhood caries. Despite the efforts focused on decreasing early childhood caries in American Indian (AI) populations, these children have the highest incidence of dental caries of any ethnic group, with four times the cases of untreated dental caries compared to white children. This qualitative formative assessment was conducted in two AI communities. Semi-structured interviews (n = 57) were conducted with caregivers and providers to understand the social and community contexts in which oral health behaviors and practices occur from the perspective of the caregivers, oral health care providers, and social service providers in the communities. The analysis was informed by the social determinants of health framework. The key social determinants of pediatric oral health relevant to our study communities included limited access to: oral health promoting nutritious foods, transportation for oral health appointments, and pediatric specialty care. This formative assessment provided locally and contextually relevant information to shape the development of an oral health clinical trial intervention to address early childhood caries in these two communities.

scholarly journals Correlation between dental caries and hormonal, biochemical parameters in children with early childhood caries

2020 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Ahmet Aras ◽  
Mehmet Sinan Doğan
Keyword(s):  
Early Childhood ◽  
Uric Acid ◽  
Statistical Analysis ◽  
Dental Caries ◽  
General Anaesthesia ◽  
Biochemical Parameters ◽  
Early Childhood Caries ◽  
Pediatric Patients ◽  
Radiographic Examination ◽  
Childhood Caries

Aim: The aim of this study was to analyze the relationship between dental caries and hormonal, biochemical parameters in pediatric patients with early childhood caries (ECC). Methodology: This study was conducted on fifty pediatric patients who presented with complaints of pain and caries and underwent dental treatment under general anaesthesia. In this study, the hormonal, biochemical parameters (glucose, urea, uric acid, creatinine, AST, ALT, GGT, CRP, Sodium, Potassium, Calcium, Glucose, Iron, TSH, FT3 and FT4) which were routinely taken from patients before general anaesthesia and the number of decayed, extracted and filled teeth (DMFT) obtained as a result of clinical and radiographic examination were evaluated statistically in patients aged 2-6 years.  Statistical analysis was performed using Independent Samples T-Test and Spearman correlation coefficient at a 0.05% significance level. Results: According to the data obtained, DMFT average of fifty children (male: 35; female: 15) included in the study was found to be 8.26. The mean DMFT was 7.93 in females and 8.40 in males. No statistical relationship was found between DMFT and gender in statistical analysis. (p>0.05). Uric acid and GGT values, which are among the hormonal and biochemical parameters, were found to be outside the normal range in 61.22% of the patients. However, statistical analysis revealed that there was no significant relationship between DMFT and hormonal, biochemical parameters. (p>0.05). Conclusions: In this study, it was concluded that dental caries did not cause a significant change in hormonal and biochemical blood parameters in pediatric patients with ECC.   How to cite this article: Aras A, Doğan MS. Correlation between dental caries and hormonal, biochemical parameters in children with early childhood caries. Int Dent Res 2020;10(1):1-5. https://doi.org/10.5577/intdentres.2020.vol10.no1.1   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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