Phospholipid Furan Fatty Acids and Ubiquinone-8: Lipid Biomarkers That May Protect Dehalococcoides Strains from Free Radicals

ABSTRACT Dehalococcoides species have a highly restricted lifestyle and are only known to derive energy from reductive dehalogenation reactions. The lipid fraction of two Dehalococcoides isolates, strains BAV1 and FL2, and a tetrachloroethene-to-ethene-dechlorinating Dehalococcoides-containing consortium were analyzed for neutral lipids and phospholipid fatty acids. Unusual phospholipid modifications, including the replacement of unsaturated fatty acids with furan fatty acids, were detected in both Dehalococcoides isolates and the mixed culture. The following three furan fatty acids are reported as present in bacterial phospholipids for the first time: 9-(5-pentyl-2-furyl)-nonanoate (Fu18:2ω6), 9-(5-butyl-2-furyl)-nonanoate (Fu17:2ω5), and 8-(5-pentyl-2-furyl)-octanoate (Fu17:2ω6). The neutral lipids of the Dehalococcoides cultures contained unusually large amounts of benzoquinones (i.e., ubiquinones [UQ]), which is unusual for anaerobes. In particular, the UQ-8 content of Dehalococcoides was 5- to 20-fold greater than that generated in aerobically grown Escherichia coli cultures relative to the phospholipid fatty acid content. Naphthoquinone isoprenologues (MK), which are often found in anaerobically grown bacteria and archaea, were also detected. Dehalococcoides shows a difference in isoprenologue pattern between UQ-8 and MK-5 that is atypical of other bacteria capable of producing both quinone types. The difference in UQ-8 and MK-5 isoprenologue patterns strongly suggests a special function for UQ in Dehalococcoides, and Dehalococcoides may utilize structural modifications in its lipid armamentarium to protect against free radicals that are generated in the process of reductive dechlorination.

Download Full-text

Les lipides des différentes catégories de pépins de Cabernet Sauvignon au moment de la véraison

OENO One ◽

10.20870/oeno-one.1980.14.3.1376 ◽

1980 ◽

Vol 14 (3) ◽

pp. 147

Author(s):

J. J. Lavaud ◽

Monique Cherrad

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Acid Content ◽

Neutral Lipids ◽

Fatty Acid Content ◽

Polar Lipids ◽

Cabernet Sauvignon ◽

Acides Gras ◽

The Difference

Cinq catégories de pépins de Cabernet Sauvignon ont été séparées à la véraison. Plus de 95 p. 100 des acides gras sont contenus dans les lipides neutres. La différence de composition en acides gras des lipides polaires montre que la localisation des pépins dans une seule loge ou dans deux loges séparées a pour conséquence une modification du fonctionnement des désaturases.+++Five categories of Cabernet Sauvignon seeds were separated at the beginning of ripening. The neutral lipids contain more than 95 per cent of the fatty acids. The difference in the fatty acid content of polar lipids shows that seeds localized in just one section or in two separate sections results in a modification of the « desaturase » functioning.

Download Full-text

WASTE-FREE TECHNOLOGY OF CHLORELLA SOROKINIANA MICROALGAE BIOMASS USAGE FOR LIPIDS AND SORBENTS PRODUCTION

IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENIY KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA ◽

10.6060/ivkkt.20186112.5795 ◽

2018 ◽

Vol 61 (12) ◽

pp. 137-143 ◽

Cited By ~ 2

Author(s):

Nataliya А. Politaeva ◽

Irina V. Atamanyuk ◽

Yuliya А. Smyatskaya ◽

Tatiana A. Kuznetsova ◽

Toumi Amira ◽

...

Keyword(s):

Fatty Acids ◽

Unsaturated Fatty Acids ◽

Human Life ◽

Saturated Fatty Acids ◽

Lipid Fraction ◽

Fatty Acid Content ◽

Biologically Active ◽

Sorption Properties ◽

Residual Biomass ◽

Microalgae Biomass

In the present article we introduce application areas of Сhlorella sorokiniana microalgae biomass for national economy (feed and biologically active additives, etc). Here it is shown that thermal and chemical modification of plant material waste might result in obtaining of highly-efficient materials for water purification. Major stages of waste-free technology of microalga processing with further extraction of valuable lipids and obtaining of sorption materials are schematically shown. We have determined fatty-acid content of lipid fraction (77 mg/g), obtained by Soxhlet method from lyophilized microalga biomass. It was revealed, that it includes 83.7% of unsaturated fatty acids (generally, С18:1, С18:2, С18:3), whereas saturated fatty acids are mostly presented by С16 – С20 family. In lyophilized biomass we discovered α-linolenoic acid (Omega-3, 28.3%), which is one of the most valuable biologically active compounds, required for full-value human life and activities. Microstructure analysis of residual biomass of C. sorokiniana microalgae has shown pore surface, formed by destroyed cells. In the course of studying the sorption properties of residual biomass with the use of initial and final concentration parameters, the efficiency of purification of model water from Fe3+ ions (47.5%) was evaluated, which indicates the need to modify the sorption properties of biomass. It has been established that the degree of wastewater purification containing iron (III) compounds can be significantly increased (by 1.8 times) by forming from the residual biomass granular materials with additional introduction of chitosan in the composition, which using as a binder and a modifying additive (mass ratio 4:1).

Download Full-text

LIPIDS OF CARDIOMYOCYTES MEMBRANES STRUCTURES IN RATS AT HYPOBIOSIS

EUREKA Life Sciences ◽

10.21303/2504-5695.2016.00046 ◽

2016 ◽

Vol 1 ◽

pp. 3-8

Author(s):

Svitlana Khyzhnyak ◽

Svitlana Midyk ◽

Serhii Sysoliatin ◽

Olena Laposha

Keyword(s):

Fatty Acids ◽

Unsaturated Fatty Acids ◽

Membrane Lipids ◽

Neutral Lipids ◽

Fatty Acid Content ◽

Biologically Active ◽

Membrane Phospholipids ◽

Internal Membrane ◽

Regulatory Systems ◽

Artificial Hypobiosis

There was studied an influence of artificial hypobiosis in the conditions of hypoxia-hypercarnia in rats on the quantity of neutral lipids, phospholipids and its fatty acids in membranes structures of cardiomyocytes (microsomes and internal membranes of mitochondria). The received results – the content of lipids, individual phospholipids, cholesterol testify to the modification of lipid component of cardiomyocytes mitochondria internal membrane (less the microsomal fraction) that characterizes modulation of cellular membranes structural-functional state. There was noticed the possibility of attraction of membrane phospholipids (sphingomyelin, phosphatidylserine and phosphatidylinositol) to the signal ways activation at hypobiosis. There was revealed redistribution of fatty acids of mitochondria internal membrane at hypobiosis that leads to increase of the level of unsaturated fatty acids. There was noticed the possibility of participation of monoenic unsaturated fatty acids in protection of cellular structures from oxidizing stress and increase of the content of arachidonic and docosahexaenoic acids – precursors of biologically active substances – can be connected with its attraction to regulatory systems at hypobiosis. There is presupposed that the state of artificial hypobiosis is characterized with stress-reaction that leads to optimal reconstruction of lipid and fatty acid content of membrane lipids directed on support of cardiomyocytes functional activity.

Download Full-text

Liposomal Formulations from Phospholipids of Greek Almond Oil. Properties and Biological Activity

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-5-607 ◽

2004 ◽

Vol 59 (5-6) ◽

pp. 330-334 ◽

Cited By ~ 7

Author(s):

Fotini Malisiova ◽

Sophia Hatziantoniou ◽

Kostas Dimas ◽

Dimitrios Kletstas ◽

Costas Demetzos

Keyword(s):

Fatty Acids ◽

Unsaturated Fatty Acids ◽

Neutral Lipids ◽

Saturated Fatty Acids ◽

Lipid Fraction ◽

Octadecenoic Acid ◽

Polar Lipids ◽

Mature Seed ◽

Seed Oils ◽

Total Lipids

The seeds of the almond tree [(Prunus dulcis (Mill.) D. A. Webb. (syn. Prunus amygdalus)] were collected in two different periods of maturity and were studied for their lipid content. The total lipids (TL) were extracted by the Bligh-Dyer method and the lipid classes have been isolated by chromatographic techniques and were analyzed by HPTLC coupled with a flame ionization detector (HPTLC/FID) and GC-MS. The oils were found to be rich in neutral lipids (89.9% and 96.3% of total lipids) and low in polar lipids (10.1% and 3.7% of total lipids) for the immature and mature seed oils, respectively. The neutral lipid fraction consisted mainly of triacylglycerides whereas the polar lipids mainly consisted of phospholipids. GC-MS data showed that the main fatty acid for both oils was 9-octadecenoic acid (oleic acid). The unsaturated fatty acids were found as high as 89.4% and 89.7%, while the percentage of the saturated fatty acids was found 10.6% and 10.3% for the immature and mature seed oils, respectively. Liposomes were prepared from the isolated phospholipids using the thin lipid film methodology, and their physical properties were characterized. Cytotoxicity was found absent when assayed against normal and cancerous cell lines. These new formulations may have future applications for encapsulation and delivery of drugs and cosmetically active ingredients.

Download Full-text

Lipid Analysis of Greek Walnut Oil (Juglans regia L.)

Zeitschrift für Naturforschung C ◽

10.1515/znc-2002-1-209 ◽

2002 ◽

Vol 57 (1-2) ◽

pp. 51-56 ◽

Cited By ~ 26

Author(s):

George Tsamouris ◽

Sophia Hatziantoniou ◽

Costas Demetzos

Keyword(s):

Fatty Acids ◽

Unsaturated Fatty Acids ◽

Lipid Analysis ◽

Neutral Lipids ◽

Juglans Regia ◽

Saturated Fatty Acids ◽

Lipid Fraction ◽

Polar Lipids ◽

Total Lipids ◽

Walnut Oil

The walnut oil (Juglans regia L.) total lipids (TL) were extracted by the Bligh-Dyer method and the lipid classes have been isolated by chromatographic techniques and they were analyzed by high performance thin layer chromatography (HPTLC) /FID and GC-MS. The oil was found to be rich in neutral lipids (96.9% of total lipids) and low in polar lipids (3.1% of total lipids). The neutral lipid fraction consisted mainly of triacylglycerides whereas the polar lipids mainly consisted of sphingolipids. GC-MS data showed that the main fatty acid was linoleic acid. Unsaturated fatty acids were found as high as 85%, while the percentage of the saturated fatty acids was found 15%. Two types of liposomes were prepared from the isolated walnut oil phospholipids and characterized as new formulations. These formulations may have future applications for encapsulation and delivery of drugs and cosmetic active ingredients.

Download Full-text

Psychosocial stress and cortisol stress reactivity predict breast milk composition

Scientific Reports ◽

10.1038/s41598-021-90980-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Anna Ziomkiewicz ◽

Magdalena Babiszewska ◽

Anna Apanasewicz ◽

Magdalena Piosek ◽

Patrycja Wychowaniec ◽

...

Keyword(s):

Fatty Acids ◽

Breast Milk ◽

Psychosocial Stress ◽

Stress Reactivity ◽

Unsaturated Fatty Acids ◽

Fatty Acid Content ◽

Milk Composition ◽

Stressful Events ◽

Psychological Status ◽

Long Chain

AbstractWe studied a sample of 146 Polish, exclusively breastfeeding mothers and their healthy born on time infants to explore the effect of perinatal psychosocial stress on breast milk composition. Maternal perinatal stress was assessed using Recent Life Changes Questionnaire summarizing stressful events from the previous six months. Stress reactivity was determined by administering the cold pressor test and measuring cortisol in saliva samples taken during the test. Breast milk sample was taken to measure energy, protein, fat, lactose, and fatty acid content. Analyses revealed that stress reactivity was positively associated with milk fat and long-chain unsaturated fatty acids and negatively associated with milk lactose. Perinatal psychosocial stress negatively affected energy density, fat as well as medium-chain and long-chain saturated fatty acids in milk. These results, together with previous studies, advocate monitoring maternal psychological status during the peripartum to promote breastfeeding and healthy infant nutrition.

Download Full-text

Meat Quality, Fatty Acid Content and NMR Metabolic Profile of Dorper Sheep Supplemented with Bypass Fats

Foods ◽

10.3390/foods10051133 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1133

Author(s):

Atique Ahmed Behan ◽

Muhammad Tayyab Akhtar ◽

Teck Chwen Loh ◽

Sharida Fakurazi ◽

Ubedullah Kaka ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Meat Quality ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Basal Diet ◽

Negative Influence ◽

Significant Difference ◽

Dorper Sheep

The supplementation of rumen bypass fat (RBF) has remained one of the preferred approaches used to decrease undesirable saturated fatty acids (FA) and increase beneficial unsaturated FA in the meat. This study was planned to evaluate the influences of rumen bypass fats on meat quality, fatty acid and metabolic profiles in male Dorper sheep (n = 36) with 24.66 ± 0.76 kg (mean ± standard error) initial body weight. Treatment comprised a basal diet (30:70 rice straw to concentrate) with no added RBF as a control (CON), basal diet with prilled fat (PF), basal diet with prilled fat plus lecithin (PFL) and basal diet with calcium soap of palm fatty acids (CaS). The findings revealed that cooking loss, drip loss and shear force in longissimus dorsi (LD) muscle were not affected by RBF supplementation, while meat pH was significantly higher in the CaS on aging day 1. However, the diet supplemented with prilled fat and lecithin modified the meat’s fatty acid profile significantly by increasing unsaturated fatty acids and decreasing saturated fats. The relative quantification of the major differentiating metabolites found in LD muscle of sheep showed that total cholesterol, esterified cholesterol, choline, glycerophosphocholine and glycerophospholipids were significantly lower in CaS and PFL diets, while glycerol and sphingomyelin were significantly higher in CaS and PFL diets. Most of the metabolites in the liver did not show any significant difference. Based on our results, the supplementation of protected fats did not have a negative influence on meat quality and the meat from Dorper sheep fed prilled fat with lecithin contained more healthy fatty acids compared to other diets.

Download Full-text

Modifying egg fatty acid content by supplementation of laying hen diets with palm olein oil (POO)

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200030519 ◽

2009 ◽

Vol 2009 ◽

pp. 212-212

Author(s):

S J Hosseini Vashan ◽

N Afzali ◽

A Golian ◽

M Malekaneh ◽

A Allahressani

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Acid Content ◽

Palm Olein ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Processing Plant ◽

Optimum Level ◽

Olein Oil

Palm oil is the most abundant of all oils produced globally. It is very high in saturated fatty acids specifically palmitic acid, but other fatty acids (monounsaturated (MUFA) and polyunsaturated) are presented at low concentrations. In the processing plant some high amount of oleic acid with some other unsaturated fatty acids are extracted and marketed as Palm olein oil, and used to reduce blood or egg cholesterol (Rievelles et al., 1994). The objective of this study was to determine the optimum level of dietary palm olein oil required to enrich the mono-unsaturated fatty acid content of yolk, egg cholesterol and antibody titre.

Download Full-text

Fatty acid positional distribution (sn-2 fatty acids) and phospholipid composition in Chinese breast milk from colostrum to mature stage

British Journal Of Nutrition ◽

10.1017/s0007114518002994 ◽

2018 ◽

Vol 121 (1) ◽

pp. 65-73 ◽

Cited By ~ 9

Author(s):

Ke Wu ◽

Runying Gao ◽

Fang Tian ◽

Yingyi Mao ◽

Bei Wang ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Linoleic Acid ◽

Fatty Acid Profile ◽

Unsaturated Fatty Acids ◽

Fatty Acid Content ◽

Positional Distribution ◽

Phospholipid Composition ◽

Mature Stage ◽

Feeding Time

AbstractThis study quantified the fatty acid profile with emphasis on the stereo-specifically numbered (sn) 2 positional distribution in TAG and the composition of main phospholipids at different lactation stages. Colostrum milk (n 70), transitional milk (n 96) and mature milk (n 82) were obtained longitudinally from healthy lactating women in Shanghai. During lactation, total fatty acid content increased, with SFA dominating in fatty acid profile. A high ratio of n-6:n-3 PUFA was observed as 11:1 over lactation due to the abundance of linoleic acid in Chinese human milk. As the main SFA, palmitic acid showed absolute sn-2 selectivity, while oleic acid, linoleic acid and α-linolenic acid, the main unsaturated fatty acids, were primarily esterified at the sn-1 and sn-3 positions. Nervonic acid and C22 PUFA including DHA were more enriched in colostrum with an sn-2 positional preference. A total of three dominant phospholipids (phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM)) were analysed in the collected samples, and each showed a decline in amount over lactation. PC was the dominant compound followed by SM and PE. With prolonged breast-feeding time, percentage of PE in total phospholipids remained constant, but PC decreased, and SM increased. Results from this study indicated a lipid profile different from Western reports and may aid the development of future infant formula more suitable for Chinese babies.

Download Full-text

Inhibition of ovine gastric acid secretion by intraduodenal long-chain fatty acids

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1982.243.2.g127 ◽

1982 ◽

Vol 243 (2) ◽

pp. G127-G133

Author(s):

L. M. McLeay ◽

J. M. Fitzgerald

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Acid Secretion ◽

Gastric Acid Secretion ◽

Gastric Acid ◽

Unsaturated Fatty Acids ◽

Acid Output ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Conscious Sheep

Effects on ovine gastric function of procedures that increase intestinal unsaturated fatty acid content are unknown, and the present aim was to compare the effects of duodenal unsaturated and saturated fatty acids on gastric secretion in conscious sheep. During the maximal gastric secretory response to a meal, 10 ml gallbladder bile alone or with myristic, palmitic, and stearic acids and oleic, linoleic, and linolenic acids were infused into the duodenum at a rate of 5 g fatty acid . h-1 for 1 h. Compared with control 154 mM NaCl (100%), acid output was reduced to 4-7% of control with infusion of oleic, linoleic, and linolenic acids and myristic acids plus bile. Reductions in acid secretion persisted for up to 5 h from the end of infusion. In contrast, the infusion of palmitic and stearic acids with bile caused mean maximal reductions in acid output, respectively, to only 64 and 55% of control, and levels returned to control within 1 h of the end of infusion. Bile infusion alone caused no reduction in acid secretion. Under the conditions used, C18 unsaturated fatty acids and myristic acid were potent inhibitors of ovine gastric acid secretion. The lesser effects of palmitic and stearic acids were probably related to their reduced solubility and absorption.

Download Full-text