scholarly journals Complete Genome Sequence of a bla CTX-M-1 -Harboring Escherichia coli Isolate Recovered from Cattle in Germany

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Jens A. Hammerl ◽  
Alexandra Irrgang ◽  
Mirjam Grobbel ◽  
Bernd-Alois Tenhagen ◽  
Annemarie Käsbohrer
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Monitoring Program ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Coli Isolate ◽  
E Coli ◽  
National Monitoring ◽  
Antimicrobial Resistance Genes ◽  
Basic Characteristics

ABSTRACT We describe here the whole-genome sequence and basic characteristics of Escherichia coli isolate 15-AB01393, recovered from German beef within a national monitoring program in 2015. This isolate was identified as an extended-spectrum-β-lactamase-producing E. coli strain of multilocus sequence type (MLST) ST58 harboring the antimicrobial resistance genes bla CTX-M-1 , mph (A), sul2 , dfrA5 , strA , and strB .

scholarly journals Genome Sequence of Escherichia coli Clone O25:H4 Sequence Type 131, Isolated from a Sudanese Patient with Urinary Tract Infection

2020 ◽  
Vol 9 (11) ◽  
Author(s):  
Sofia B. Mohamed ◽  
Mohamed M. Hassan ◽  
Sumaya Kambal ◽  
Abdalla Munir ◽  
Nusiba I. Abdalla ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Genome Sequence ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
E Coli

We report here the whole-genome sequence of Escherichia coli NUBRI-E, a representative of E. coli clone O25:H4 sequence type 131 with bla CTX-M-15, which was obtained from a Sudanese patient with a urinary tract infection.

scholarly journals Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

2020 ◽  
Vol 9 (1) ◽  
pp. 29
Author(s):  
Natalie Pauly ◽  
Jens Andre Hammerl ◽  
Mirjam Grobbel ◽  
Annemarie Käsbohrer ◽  
Bernd-Alois Tenhagen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Chain ◽  
Phylogenetic Group ◽  
Sequence Type ◽  
Selective Isolation ◽  
Coli Isolate ◽  
E Coli ◽  
National Monitoring ◽  
German Food ◽  
Human Source

Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock. In this monitoring in 2019, the CP E. coli 19-AB01133 was recovered from pork shoulder. The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869. Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes blaVIM-1, blaSHV-5 and blaCMY-13 on a self-transmissible IncA/C2 plasmid. The plasmid was closely related to the previously described VIM-1-encoding plasmid S15FP06257_p from E. coli of pork origin in Belgium. Our results indicate an occasional spread of the blaVIM-1 gene in Enterobacteriaceae of the European pig population. Moreover, the blaVIM-1 located on an IncA/C2 plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.

scholarly journals Draft Genome Sequence of a Multidrug- and Colistin-Resistant mcr-1 -Producing Escherichia coli Isolate from a Swine Farm in Mexico

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Ulises Garza-Ramos ◽  
Elsa Tamayo-Legorreta ◽  
Doris María Arellano-Quintanilla ◽  
Nadia Rodriguez-Medina ◽  
Jesús Silva-Sanchez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Swine Farm ◽  
Content Type ◽  
Coli Isolate ◽  
E Coli

ABSTRACT A colistin-resistant mcr-1 -carrying Escherichia coli strain, RC2-007, was isolated from a swine farm in Mexico. This extraintestinal and uropathogenic strain of E. coli belongs to serotype O89:H9 and sequence type 744. Assembly and annotation resulted in a 4.9-Mb draft genome that revealed the presence of plasmid-mediated mcr-1 -IS ApI1 genes as part of a prophage.

scholarly journals Draft Genome Sequence of the Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolate INF13/18/A, Recovered from Kelantan, Malaysia

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Wan Ratmaazila Wan Makhtar ◽  
Mawaddah Mohd Azlan ◽  
Nor Haslizai Hassan ◽  
Ismail Aziah ◽  
Nurul Hidayah Samsurizal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Coli Isolate ◽  
Extended Spectrum ◽  
Antimicrobial Resistance Genes ◽  
Basic Characteristics

ABSTRACT We describe here the draft genome sequence and basic characteristics of Escherichia coli isolate INF13/18/A, which was isolated from Universiti Sains Malaysia (USM) Hospital. This isolate was identified as an extended-spectrum β-lactamase-producing Escherichia coli strain harboring the antimicrobial resistance genes TEM, CTX-M-1, and CTX-M-9.

scholarly journals Whole-Genome Sequence of Phage-Resistant Strain Escherichia coli DH5α

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Jingchao Chen ◽  
Yi Li ◽  
Kun Zhang ◽  
Hailei Wang
Keyword(s):  
Escherichia Coli ◽  
Comparative Analysis ◽  
Genome Sequence ◽  
Resistant Strain ◽  
Resistance Mechanism ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
E Coli ◽  
T4 Bacteriophage

ABSTRACT The genomes of many strains of Escherichia coli have been sequenced, as this organism is a classic model bacterium. Here, we report the genome sequence of Escherichia coli DH5α, which is resistant to a T4 bacteriophage (CCTCC AB 2015375), while its other homologous E. coli strains, such as E. coli BL21, DH10B, and MG1655, are not resistant to phage invasions. Thus, understanding of the genome of the DH5α strain, along with comparative analysis of its genome sequence along with other sequences of E. coli strains, may help to reveal the bacteriophage resistance mechanism of E. coli .

scholarly journals Salmonella Genomic Island 1B Variant Found in a Sequence Type 117 Avian Pathogenic Escherichia coli Isolate

mSphere ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Max Laurence Cummins ◽  
Piklu Roy Chowdhury ◽  
Marc Serge Marenda ◽  
Glenn Francis Browning ◽  
Steven Philip Djordjevic
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Acinetobacter Baumannii ◽  
Genome Sequencing ◽  
Genomic Island ◽  
Sequence Type ◽  
Genomic Islands ◽  
Content Type ◽  
E Coli ◽  
Antimicrobial Resistance Genes

ABSTRACT Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and several other genera. The island typically confers resistance to older, first-generation antimicrobials; however, some variants carry blaNDM-1, blaVEB-6, and blaCTX-M15 genes that encode resistance to frontline, clinically important antibiotics, including third-generation cephalosporins. Genome sequencing studies of avian pathogenic Escherichia coli (APEC) identified a sequence type 117 (ST117) isolate (AVC96) with genetic features found in SGI1. The complete genome sequence of AVC96 was assembled from a combination of Illumina and single-molecule real-time (SMRT) sequence data. Analysis of the AVC96 chromosome identified a variant of SGI1-B located 18 bp from the 3′ end of trmE, also known as the attB site, a known hot spot for the integration of genomic islands. This is the first report of SGI1 in wild-type E. coli. The variant, here named SGI1-B-Ec1, was otherwise unremarkable, apart from the identification of ISEc43 in open reading frame (ORF) S023. IMPORTANCE SGI1 and variants of it carry a variety of antimicrobial resistance genes, including those conferring resistance to extended-spectrum β-lactams and carbapenems, and have been found in diverse S. enterica serovars, Acinetobacter baumannii, and other members of the Enterobacteriaceae. SGI1 integrates into Gram-negative pathogenic bacteria by targeting a conserved site 18 bp from the 3′ end of trmE. For the first time, we describe a novel variant of SGI1 in an avian pathogenic Escherichia coli isolate. The presence of SGI1 in E. coli is significant because it represents yet another lateral gene transfer mechanism to enhancing the capacity of E. coli to acquire and propagate antimicrobial resistance and putative virulence genes. This finding underscores the importance of whole-genome sequencing (WGS) to microbial genomic epidemiology, particularly within a One Health context. Further studies are needed to determine how widespread SGI1 and variants of it may be in Australia.

scholarly journals Molecular Characterization ofblaNDM-5Carried on an IncFII Plasmid in an Escherichia coli Isolate from a Nontraveler Patient in Spain

2014 ◽  
Vol 59 (1) ◽  
pp. 659-662 ◽  
Author(s):  
Cristina Pitart ◽  
Mar Solé ◽  
Ignasi Roca ◽  
Angely Román ◽  
Asunción Moreno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Female Patient ◽  
Urine Culture ◽  
Sequence Type ◽  
Content Type ◽  
Pcr Screening ◽  
Coli Isolate ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Indian Origin

ABSTRACTA carbapenem-resistantEscherichia coliisolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence ofblaNDM-5,blaTEM-1,blaOXA-1,blaCMY-42, andrmtB. blaNDM-5was carried in a conjugative IncFII-type plasmid (90 kb) together withblaTEM-1andrmtB. The genetic environment ofblaNDM-5showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France inE. coliof African and Indian origin, respectively.

scholarly journals Complete Genome Sequence of Escherichia coli 81009, a Representative of the Sequence Type 131 C1-M27 Clade with a Multidrug-Resistant Phenotype

2018 ◽  
Vol 6 (8) ◽  
Author(s):  
Michele Mutti ◽  
Ágnes Sonnevend ◽  
Tibor Pál ◽  
Sini Junttila ◽  
Heinz Ekker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Significant Proportion ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
E Coli ◽  
Resistant Phenotype

ABSTRACT The sequence type 131 (ST131)- H 30 clone is responsible for a significant proportion of multidrug-resistant extraintestinal Escherichia coli infections. Recently, the C1-M27 clade of ST131- H 30, associated with bla CTX-M-27 , has emerged. The complete genome sequence of E. coli isolate 81009 belonging to this clone, previously used during the development of ST131-specific monoclonal antibodies, is reported here.

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