scholarly journals Draft Genome Sequences of Six Novel Picorna-Like Viruses from Washington State Spiders

2017 ◽  
Vol 5 (9) ◽  
Author(s):  
Ryan C. Shean ◽  
Negar Makhsous ◽  
Rodney L. Crawford ◽  
Keith R. Jerome ◽  
Alexander L. Greninger
Keyword(s):  
Amino Acid ◽  
Viral Genome ◽  
Draft Genome ◽  
Amino Acid Identity ◽  
Washington State ◽  
Genome Sequences ◽  
Acid Identity ◽  
Spider Species ◽  
Viral Sequences

ABSTRACT We report draft genome sequences of six novel Picornavirales members from six different spider species found in Washington state. These six viral sequences distinctly clustered together phylogenetically with less than 35% amino acid identity to the closest reference viral genome.

scholarly journals Isolation of a Complete Circular Virus Genome Sequence from an Alaskan Black-Capped Chickadee ( Poecile atricapillus ) Gastrointestinal Tract Sample

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Zachary R. Hanna ◽  
Charles Runckel ◽  
Jérôme Fuchs ◽  
Joseph L. DeRisi ◽  
David P. Mindell ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gastrointestinal Tract ◽  
Genome Sequence ◽  
Amino Acid Identity ◽  
Virus Genome ◽  
Genome Sequences ◽  
Poecile Atricapillus ◽  
Single Stranded Dna ◽  
Acid Identity

We report here the genome sequence of a circular virus isolated from samples of an Alaskan black-capped chickadee ( Poecile atricapillus ) gastrointestinal tract. The genome is 2,152 bp in length and is most similar (30 to 44.5% amino acid identity) to the genome sequences of other single-stranded DNA (ssDNA) circular viruses belonging to the gemycircularvirus group.

scholarly journals Draft Genome Sequence of Clinical Isolate Alcaligenaceae sp. Strain 429

2019 ◽  
Vol 8 (19) ◽  
Author(s):  
Michael J. Zilliox ◽  
Paul C. Schreckenberger ◽  
Catherine Putonti
Keyword(s):  
Amino Acid ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Draft Genome ◽  
Amino Acid Identity ◽  
Unknown Etiology ◽  
Rrna Gene ◽  
Acid Identity ◽  
Average Amino Acid Identity ◽  
The 16S Rrna Gene

Here, we present the 3.53-Mb genome for Alcaligenaceae sp. strain 429, isolated from a patient with unknown etiology. While the 16S rRNA gene most closely resembles Paenalcaligenes species, average amino acid identity (AAI) analysis did not meet the threshold to classify our strain as a species of this family.

scholarly journals Draft Genome Sequence of a Novel Picornavirus Isolated from Japanese Eel (Anguilla japonica)

2020 ◽  
Vol 9 (42) ◽  
Author(s):  
Sachiko Terashima ◽  
Motoo Suzuki ◽  
Tomokazu Takano ◽  
Motoshige Yasuike ◽  
Tomomasa Matsuyama ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genome Sequence ◽  
Draft Genome ◽  
Amino Acid Identity ◽  
Anguilla Japonica ◽  
Japanese Eel ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Acid Identity

ABSTRACT We report the draft genome sequence of a novel member of the order Picornavirales that was obtained from the gills of farmed Japanese eel (Anguilla japonica). A putative polyprotein encoded by the genome was similar to that of other picornaviruses and shared 31% amino acid identity with that of eel picornavirus 1.

scholarly journals Genome Sequence of Hardyhisp2, a Gammapleolipovirus Infecting Haloarcula hispanica

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Mike Dyall-Smith ◽  
Friedhelm Pfeiffer ◽  
Pei-Wen Chiang ◽  
Sen-Lin Tang
Keyword(s):  
Amino Acid ◽  
Viral Genome ◽  
Amino Acid Identity ◽  
Spike Protein ◽  
Inverted Repeats ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Terminal Inverted Repeats ◽  
Acid Identity ◽  
Haloarcula Hispanica

ABSTRACT Hardyhisp2 virus infects the halophilic archaeon Haloarcula hispanica DSM 4426T and is closely related to His2 (Pleolipoviridae family). The viral genome is 16,133 bp long, with terminal inverted repeats of 599 bp. The predicted spike protein is only weakly similar (32% amino acid identity) to that of His2.

scholarly journals A50 Whole-genome sequencing of African swine fever isolates from Sardinia

2019 ◽  
Vol 5 (Supplement_1) ◽  
Author(s):  
C Torresi ◽  
F Granberg ◽  
L Bertolotti ◽  
A Oggiano ◽  
B Colitti ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Data ◽  
Temporal Distribution ◽  
African Swine Fever ◽  
Amino Acid Identity ◽  
Genotype I ◽  
Acid Identity ◽  
Wide Range ◽  
Intergenic Sequences ◽  
Genes Encoding

Abstract In order to assess the molecular epidemiology of African swine fever (ASF) in Sardinia, we analyzed a wide range of isolates from wild and domestic pigs over a 31-year period (1978–2009) by genotyping sequence data from the genes encoding the p54 and the p72 proteins and the CVR. On this basis, the analysis of the B602L gene revealed a minor difference, placing the Sardinian isolates into two clusters according to their temporal distribution. As an extension of this study, in order to achieve a higher level of discrimination, three further variable genome regions, namely p30, CD2v, and I73R/I329L, of a large number of isolates collected from outbreaks in the years 2002–14 have been investigated. Sequence analysis of the CD2v region revealed a temporal subdivision of the viruses into two subgroups. These data, together with those from the B602L gene analysis, demonstrated that the viruses circulating in Sardinia belong to p72/genotype I, but since 1990 have undergone minor genetic variations in respect to its ancestor, thus making it impossible to trace isolates, enabling a more accurate assessment of the origin of outbreaks, and extending knowledge of virus evolution. To solve this problem, we have sequenced and annotated the complete genome of nine ASF isolates collected in Sardinia between 1978 and 2012. This was achieved using sequence data determined by next-generation sequencing. The results showed a very high identity with range of nucleotide similarity among isolates of 99.5 per cent to 99.9 per cent. The ASF virus (ASFV) genomes were composed of terminal inverted repeats and conserved and non-conserved ORFs. Among the conserved ORFs, B385R, H339R, and O61R-p12 showed 100 per cent amino acid identity. The same was true for the hypervariable ORFs, with regard to X69R, DP96R, DP60R, EP153R, B407L, I10L, and L60L genes. The EP402R and B602L genes showed, as expected, an amino acid identity range of 98.5 per cent to 100 per cent and 91 per cent to 100 per cent, respectively. In addition, all of the isolates displayed variable intergenic sequences. As a whole, the results from our studies confirmed a remarkable genetic stability of the ASFV/p72 genotype I viruses circulating in Sardinia.

Characterization and Induction of Two Cytochrome P450 Genes, CYP6AE28 and CYP6AE30, in Cnaphalocrocis medinalis: Possible Involvement in Metabolism of Rice Allelochemicals

2010 ◽  
Vol 65 (11-12) ◽  
pp. 719-725 ◽  
Author(s):  
Xiaoli Liu ◽  
Jun Chen ◽  
Zhifan Yang
Keyword(s):  
Amino Acid ◽  
Rice Variety ◽  
Amino Acid Identity ◽  
Cnaphalocrocis Medinalis ◽  
Amino Acid Residues ◽  
Acid Identity ◽  
Rice Leaf Folder ◽  
Cytochrome P450 Genes ◽  
Molecular Masses ◽  
Lepidoptera Pyralidae

Two cDNAs specific for P450 genes, CYP6AE28 and CYP6AE30, have been isolated from the rice leaf folder Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae). Both cDNApredicted proteins have 504 amino acid residues in length, but with molecular masses of 60177 Dalton for CYP6AE28 and 60020 Dalton for CYP6AE30, and theoretical pI values of 8.49 for CYP6AE28 and 8.56 for CYP6AE30, respectively. Both putative proteins contain the conserved structural and functional domains characteristic of all CYP6 members. CYP6AE28 and CYP6AE30 show 52% amino acid identity to each other; both of them have 49 - 56% identities with CYP6AE1, Cyp6ae12, and CYP6AE14. Phylogenetic analysis showed that the two P450s are grouped in the lineage containing some of the CYP6AE members, CYP6B P450s and CYP321A1. The transcripts of CYP6AE28 and CYP6AE30 were found to be induced in response to TKM-6, a rice variety with high resistance to C. medinalis. The results suggest that the two P450s may play important roles in adaptation to the host plant rice. This is the first report of P450 genes cloned in C. medinalis

scholarly journals Draft Genome Sequences of 12 Clinical and Environmental Methicillin-Resistant Staphylococcus pseudintermedius Strains Isolated from a Veterinary Teaching Hospital in Washington State

2018 ◽  
Vol 6 (15) ◽  
pp. e00290-18
Author(s):  
Devendra H. Shah ◽  
Lisa P. Jones ◽  
Narayan Paul ◽  
Margaret A. Davis
Keyword(s):  
Teaching Hospital ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Washington State ◽  
Nosocomial Transmission ◽  
Whole Genome ◽  
Genome Sequences ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type

ABSTRACT Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a globally emergent multidrug-resistant pathogen of dogs associated with nosocomial transmission in dogs and with potential zoonotic impacts. Here, we report the draft whole-genome sequences of 12 hospital-associated MRSP strains and their resistance genotypes and phenotypes.

scholarly journals PFM-Like Enzymes Are a Novel Family of Subclass B2 Metallo-β-Lactamases from Pseudomonas synxantha Belonging to the Pseudomonas fluorescens Complex

2019 ◽  
Vol 64 (2) ◽  
Author(s):  
Laurent Poirel ◽  
Mattia Palmieri ◽  
Michael Brilhante ◽  
Amandine Masseron ◽  
Vincent Perreten ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pseudomonas Fluorescens ◽  
Bacterial Species ◽  
Amino Acid Identity ◽  
Chicken Meat ◽  
The Novel ◽  
Content Type ◽  
Acid Identity ◽  
Carbapenem Resistant ◽  
Encoding Genes

ABSTRACT A carbapenem-resistant Pseudomonas synxantha isolate recovered from chicken meat produced the novel carbapenemase PFM-1. That subclass B2 metallo-β-lactamase shared 71% amino acid identity with β-lactamase Sfh-1 from Serratia fonticola. The blaPFM-1 gene was chromosomally located and likely acquired. Variants of PFM-1 sharing 90% to 92% amino acid identity were identified in bacterial species belonging to the Pseudomonas fluorescens complex, including Pseudomonas libanensis (PFM-2) and Pseudomonas fluorescens (PFM-3), highlighting that these species constitute reservoirs of PFM-like encoding genes.

Comparative sequence analysis of morbillivirus receptors and its implication in host range expansion

2019 ◽  
Vol 65 (11) ◽  
pp. 783-794
Author(s):  
Ajay Kumar Yadav ◽  
Kaushal Kishor Rajak ◽  
Mukesh Bhatt ◽  
Ashok Kumar ◽  
Soumendu Chakravarti ◽  
...  
Keyword(s):  
Amino Acid ◽  
Host Range ◽  
Range Expansion ◽  
Small Ruminants ◽  
Amino Acid Identity ◽  
Amino Acid Residues ◽  
Binding Region ◽  
Acid Identity ◽  
Host Range Expansion ◽  
High Amino Acid

SLAM (CD150) and nectin-4 are the major morbillivirus receptors responsible for virus pathogenesis and host range expansion. Recently, morbillivirus infections have been reported in unnatural hosts, including endangered species, posing a threat to their conservation. To understand the host range expansion of morbilliviruses, we generated the full-length sequences of morbillivirus receptors (goat, sheep, and dog SLAM, and goat nectin-4) and tried to correlate their role in determining host tropism. A high level of amino acid identity was observed between the sequences of related species, and phylogenetic reconstruction showed that the receptor sequences of carnivores, marine mammals, and small ruminants grouped separately. Analysis of the ligand binding region (V region; amino acid residues 52–136) of SLAM revealed high amino acid identity between small ruminants and bovine SLAMs. Comparison of canine SLAM with ruminants and non-canids SLAM revealed appreciable changes, including charge alterations. Significant differences between feline SLAM and canine SLAM have been reported. The binding motifs of nectin-4 genes (FPAG motif and amino acid residues 60, 62, and 63) were found to be conserved in sheep, goat, and dog. The differences reported in the binding region may be responsible for the level of susceptibility or resistance of a species to a particular morbillivirus.

Sign in / Sign up

Export Citation Format

Share Document