Study ofLegionellaEffector Domains Revealed Novel and Prevalent Phosphatidylinositol 3-Phosphate Binding Domains
ABSTRACTLegionella pneumophilaand otherLegionellaspecies replicate intracellularly using the Icm/Dot type IV secretion system. InL. pneumophilathis system translocates >300 effectors into host cells and in theLegionellagenus thousands of effectors were identified, the function of most of which is unknown. FourteenL. pneumophilaeffectors were previously shown to specifically bind phosphoinositides (PIs) using dedicated domains. We found that PI-binding domains of effectors are usually not homologous to one another; they are relatively small and located at the effectors' C termini. We used the previously identifiedLegionellaeffector domains (LEDs) with unknown function and the above characteristics of effector PI-binding domains to discover novel PI-binding LEDs. We identified three predicted PI-binding LEDs that are present in 14 L. pneumophilaeffectors and in >200 effectors in theLegionellagenus. Using anin vitroprotein-lipid overlay assay, we found that 11 of theseL. pneumophilaeffectors specifically bind phosphatidylinositol 3-phosphate (PI3P), almost doubling the number ofL. pneumophilaeffectors known to bind PIs. Further, we identified in each of these newly discovered PI3P-binding LEDs conserved, mainly positively charged, amino acids that are essential for PI3P binding. Our results indicate thatLegionellaeffectors harbor unique domains, shared by many effectors, which directly mediate PI3P binding.