Isolation and characterization of the rRNA gene clusters of Halobacterium marismortui.

We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L−1, with a first-order rate constant of 0.6 d−1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium.

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Isolation and Characterization of Levoglucosan Metabolizing Bacteria

Applied and Environmental Microbiology ◽

10.1128/aem.01868-21 ◽

2021 ◽

Author(s):

Ajay S. Arya ◽

Minh T. H. Hang ◽

Mark A. Eiteman

Keyword(s):

Recombinant Expression ◽

Bacterial Species ◽

Soluble Carbohydrate ◽

Rrna Gene ◽

Gene Products ◽

16S Rrna Gene Sequences ◽

E Coli ◽

Isolation And Characterization ◽

Charred Wood

Bacteria were isolated from wastewater and soil containing charred wood remnants based on their ability to use levoglucosan as a sole carbon source and on their levoglucosan dehydrogenase (LGDH) activity. On the basis of their 16S rRNA gene sequences, these bacteria represented diverse genera of Microbacterium, Paenibacillus , Shinella , and Klebsiella . Genomic sequencing of the isolates verified that two isolates represented novel species, Paenibacillus athensensis MEC069 T and Shinella sumterensis MEC087 T , while the remaining isolates were closely related to either Microbacterium lacusdiani or Klebsiella pneumoniae . The genetic sequence of LGDH, lgdA , was found in the genomes of these four isolates as well as Pseudarthrobacter phenanthrenivorans Sphe3. The identity of the P. phenanthrenivorans LGDH was experimentally verified following recombinant expression in E. coli . Comparison of the putative genes surrounding lgdA in the isolate genomes indicated that several other gene products facilitate the bacterial catabolism of levoglucosan, including a putative sugar isomerase and several transport proteins. Importance Levoglucosan is the most prevalent soluble carbohydrate remaining after high temperature pyrolysis of lignocellulosic biomass, but it is not fermented by typical production microbes such as Escherichia coli and Saccharomyces cerevisiae . A few fungi metabolize levoglucosan via the enzyme levoglucosan kinase, while several bacteria metabolize levoglucosan via levoglucosan dehydrogenase. This study describes the isolation and characterization of four bacterial species which degrade levoglucosan. Each isolate is shown to contain several genes within an operon involved in levoglucosan degradation, furthering our understanding of bacteria which metabolize levoglucosan.

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Isolation and Characterization of Soil Myxobacteria from Nepal

Journal of Institute of Science and Technology ◽

10.3126/jist.v24i2.27246 ◽

2019 ◽

Vol 24 (2) ◽

pp. 7-16

Author(s):

Nabin Rana ◽

Saraswoti Khadka ◽

Bishnu Prasad Marasini ◽

Bishnu Joshi ◽

Pramod Poudel ◽

...

Keyword(s):

Sequence Similarity ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Rrna Gene ◽

Isolation And Characterization ◽

The Family ◽

Global Concern ◽

Bacteria And Fungi ◽

Antimicrobial Metabolites

Realizing myxobacteria as a potential source of antimicrobial metabolites, we pursued research to isolate myxobacteria showing antimicrobial properties. We have successfully isolated three strains (NR-1, NR-2, NR-3) using the Escherichia coli baiting technique. These isolates showed typical myxobacterial growth characteristics. Phylogenetic analysis showed that all the strains (NR-1, NR-2, NR-3) belong to the family Archangiaceae, suborder Cystobacterineae, and order Myxococcales. Furthermore, 16S rRNA gene sequence similarity searched through BLAST revealed that strain NR-1 showed the closest similarity (91.8 %) to the type strain Vitiosangium cumulatum (NR-156939), NR-2 showed (98.8 %) to the type of Cystobacter badius (NR-043940), and NR-3 showed the closest similarity (83.5 %) to the type of strain Cystobacter fuscus (KP-306730). All isolates showed better growth in 0.5-1 % NaCl and pH around 7.0, whereas no growth was observed at pH 9.0 and below 5.0. All strains showed better growth at 32° C and hydrolyzed starch, whereas casein was efficiently hydrolyzed by NR-1 and NR-2. Besides, preliminary antimicrobial tests from crude extracts showed activities against Gram-positive, Gram-negative bacteria, and fungi. Our findings suggest that the arcane soil habitats of Nepal harbor myxobacteria with the capability to produce diverse antimicrobial activities that may be explored to overcome the rapidly rising global concern about antibiotic resistance.

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Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River

Phytotaxa ◽

10.11646/phytotaxa.387.4.1 ◽

2019 ◽

Vol 387 (4) ◽

pp. 269 ◽

Cited By ~ 2

Author(s):

ELANE D. CUNHA DE OLIVEIRA ◽

ALAN C. DA CUNHA ◽

NATALINA B. DA SILVA ◽

RAQUEL CASTELO-BRANCO ◽

JOÃO MORAIS ◽

...

Keyword(s):

River Mouth ◽

Taxonomic Revision ◽

16S Rrna Gene Sequencing ◽

Gene Clusters ◽

Toxin Production ◽

Amazon River ◽

Rrna Gene ◽

Cyanobacterial Diversity ◽

Rrna Gene Sequencing

The Amazon region contains a great diversity of species, and the Amazon River basin accounts for almost 20% of all the freshwater in the world. Despite the favorable environmental conditions in this region, little is known about the cyanobacterial diversity of this waterbody, especially at the mouth of the river. In this paper, we used the polyphasic approach to identify 14 cyanobacterial strains isolated in the Amazon River on the inlet site from a drinking water supply located close to the river mouth. The isolated strains were characterized based on morphology, behavior in culture, 16S rRNA gene sequencing, phylogenetic analysis and potential for toxin production. The isolated strains belong to seven different genera, namely, Alkalinema, Cephalothrix, Limnothrix, Leptolyngbya, Phormidium, Pseudanabaena and an unidentified Nostocales taxa that may represent a new genus. Strikingly, there were no new species, nor detection of gene clusters associated with cyanotoxin production. However, the phylogenetic placements of the Amazonian strains of Limnothrix and Pseudanabaena provide new insight into the taxonomy of these genera, reinforcing the need for taxonomic revision.

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Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities

Polish Polar Research ◽

10.1515/popore-2016-0021 ◽

2016 ◽

Vol 37 (3) ◽

pp. 403-419 ◽

Cited By ~ 3

Author(s):

Paris Lavín ◽

Cristian Atala ◽

Jorge Gallardo-Cerda ◽

Marcelo Gonzalez-Aravena ◽

Rodrigo De La Iglesia ◽

...

Keyword(s):

Alginate Lyase ◽

Enzymatic Activities ◽

Maximum Temperature ◽

Rrna Gene ◽

King George Island ◽

Optimal Temperature ◽

16S Rrna Gene Analysis ◽

Isolation And Characterization ◽

Different Temperatures

AbstractSeveral bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures.

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Isolation and Characterization of Rhodopseudomonas sp. S9-1 Capable of Degrading Pyrazosulfuron-Ethyl

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.356-360.1152 ◽

2011 ◽

Vol 356-360 ◽

pp. 1152-1163 ◽

Cited By ~ 5

Author(s):

Le Bin Yin ◽

Yong Liu ◽

De Yong Zhang ◽

Song Bai Zhang

Keyword(s):

Contaminated Soil ◽

Acetolactate Synthase ◽

Optimal Growth ◽

Degradation Process ◽

Amino Acid Sequences ◽

Rrna Gene ◽

Photosynthetic Membrane ◽

Physiological And Biochemical Characteristics ◽

Isolation And Characterization

A bacterial strain S9-1capable of degrading sulfonylurea herbicide pyrazosulfuron-ethyl (PSE) was isolated from contaminated soil through the enrichment incubation method. Based on morphology, colony and cultural properties, physiological and biochemical characteristics, living-cell absorption spectra, internal photosynthetic membrane, and phylogenetics of its 16S rRNA gene sequence, S9-1was preliminarily identified as belonging to the genus Rhodopseudomonas, a group of photosynthetic bacteria (PSB). The effects of PSE concentration, pH, and temperature on biodegradation were examined. The degradation rate was found to decrease with increasing PSE concentration. Optimal growth pH and temperature were found to be 7.0 and 30°C, respectively. The strain was able to degrade 47.51% of PSE at a concentration of 100 mg ml-1after 7 days of incubation at 30°C and could tolerate 800 mg ml-1PSE. S9-1was also able to completely co-metabolically transform 100 mg ml-1PSE at 30°C, pH 7.0, and 7500 lux in 15 days. As the concentration of PSE increased, the degradation process took longer to complete. The fragment encoding acetolactate synthase (ALS) gene from S9-1was cloned and sequenced. Comparison of deduced amino acid sequences was implemented, and the conserved sites were analyzed. To our knowledge, this is the first report of PSB in PSE biodegradation. These results highlight the potential of this bacterium as a detoxifying agent for use with PSE-contaminated soil and wastewater.

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Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer

Current Biochemistry ◽

10.29244/cb.2.2.86-98 ◽

2015 ◽

Vol 2 (2) ◽

pp. 86-98

Author(s):

Dina Dyah Saputri ◽

Maria Bintang ◽

Fachriyan H Pasaribu

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Endophytic Bacteria ◽

Pathogenic Bacteria ◽

Lantana Camara ◽

Rrna Gene ◽

Antibacterial Compounds ◽

Isolation And Characterization ◽

Ms Analysis

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

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Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India

Journal of Applied and Natural Science ◽

10.31018/jans.v13i1.2574 ◽

2021 ◽

Vol 13 (1) ◽

pp. 396-401

Author(s):

Khushbu Parihar ◽

Alkesh Tak ◽

Praveen Gehlot ◽

Rakesh Pathak ◽

Sunil Kumar Singh

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bioactive Compounds ◽

Salt Lake ◽

Bioactive Compound ◽

Rrna Gene ◽

Biochemical Tests ◽

Multiple Sequence ◽

Isolation And Characterization

The genus Nocardiopsis is well known to produce secondary metabolites especially antibacterial bioactive compound. Isolation and characterization of bioactive compounds producing novel isolates from unusual habitats are crucial. The present study was aimed to explore Didwana dry salt lake of Rajasthan state in India for the isolation and characterization of actinomycetes. The isolated actinomycetes isolates were characterized based on culture characteristics, biochemical tests and 16S rRNA gene sequencing. The 16S rRNA gene sequence analysis revealed that all the five isolates inhabiting soil of the said dry salt lake of Didwana, Rajasthan belonged to four species of Nocardiopsis viz., N. synnemataformans, N. potens, N. prasina and N. dassonvillei subsp. albirubida. The molecular identification based on 16S rRNA gene sequences was found accurate and robust. The phylogram generated through multiple sequence alignment of all the test isolates of Nocardiopsis revealed that the isolates aroused from a single branch and validated monophyletic association. The present study is the first report of exploring Nocardiopsis isolates from the dry salt lake. These characterized Nocardiopsis isolates isolated from Didwana dry salt lake habitat are novel stains and can be of significance in the detection and utilization of novel bioactive compounds.

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Characterization of Streptomyces venezuelae ATCC 10595 rRNA gene clusters and cloning of rrnA.

Journal of Bacteriology ◽

10.1128/jb.178.5.1480-1483.1996 ◽

1996 ◽

Vol 178 (5) ◽

pp. 1480-1483 ◽

Cited By ~ 8

Author(s):

M La Farina ◽

S Stira ◽

R Mancuso ◽

C Grisanti

Keyword(s):

Gene Clusters ◽

Rrna Gene ◽

Streptomyces Venezuelae

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