Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208
1998 ◽
Vol 180
(16)
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pp. 4319-4323
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Keyword(s):
ABSTRACT In Bacillus sphaericus and other Bacillusspp., d-amino acid transaminase has been considered solely responsible for biosynthesis of d-glutamate, an essential component of cell wall peptidoglycan, in contrast to the glutamate racemase employed by many other bacteria. We report here the cloning of the dat gene encoding d-amino acid transaminase and the glr gene encoding a glutamate racemase from B. sphaericus ATCC 10208. The glrgene encodes a 28.8-kDa protein with 40 to 50% sequence identity to the glutamate racemases of Lactobacillus,Pediococcus, and Staphylococcus species. Thedat gene encodes a 31.4-kDa peptide with 67% primary sequence homology to the d-amino acid transaminase of the thermophilic Bacillus sp. strain YM1.
2004 ◽
Vol 70
(12)
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pp. 7303-7310
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Keyword(s):
2004 ◽
Vol 186
(15)
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pp. 4885-4893
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1998 ◽
Vol 36
(1)
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pp. 41-47
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Keyword(s):
2001 ◽
Vol 45
(2)
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pp. 616-620
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Keyword(s):
1999 ◽
Vol 181
(8)
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pp. 2323-2329
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Keyword(s):
1998 ◽
Vol 72
(5)
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pp. 3507-3511
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