Stable Accumulation of σ54 in Helicobacter pylori Requires the Novel Protein HP0958
ABSTRACT Several flagellar genes in Helicobacter pylori are dependent on σ54 (RpoN) for their expression. These genes encode components of the basal body, the hook protein, and a minor flagellin, FlaB. A protein-protein interaction map for H. pylori constructed from a high-throughput screen of a yeast two-hybrid assay (http://pim.hybrigenics.com/pimrider ext/common/) revealed interactions between σ54 and the conserved hypothetical protein HP0958. To see if HP0958 influences σ54 function, the corresponding gene was disrupted with a kanamycin resistance gene (aphA3) in H. pylori ATCC 43504 and the resulting mutant was analyzed. The hp0958:aphA3 mutant was nonmotile and failed to produce flagella. Introduction of a functional copy of hp0958 into the genome of the hp0958:aphA3 mutant restored flagellar biogenesis and motility. The hp0958:aphA3 mutant was deficient in expressing two σ54-dependent reporter genes, flaB′-′xylE and hp1120′-′xylE. Levels of σ54 in the hp0958 mutant were substantially lower than those in the parental strain, suggesting that the failure of the mutant to express the genes in the RpoN regulon and produce flagella was due to reduced σ54 levels. Expressing σ54 at high levels by putting rpoN under the control of the ureA promoter restored flagellar biogenesis and motility in the hp0958:aphA3 mutant. Turnover of σ54 was more rapid in the hp0958:aphA3 mutant than it was in the wild-type strain, suggesting that HP0958 supports wild-type σ54 levels in H. pylori by protecting it from proteolysis.