scholarly journals Simple procedure for the removal of nonspecific inhibitors of rubella virus hemagglutination

1975 ◽  
Vol 2 (6) ◽  
pp. 524-527
Author(s):  
R Allen ◽  
K Hedlund
Keyword(s):  
Silicic Acid ◽  
Rubella Virus ◽  
Comparative Studies ◽  
Hemagglutination Inhibition ◽  
Room Temperature ◽  
Dextran Sulfate ◽  
Simple Procedure ◽  
Serum Lipoproteins

The adsorption of serum lipoproteins onto an insoluble matrix of colloidal silicic acid results in the removal of nonspecific inhibitors of rubella virus hemagglutinin. The procedure can be performed in 15 min at room temperature. Comparative studies using both the dextran sulfate-CaCl2 and heparin-MnCl2 methods for removal of inhibitors demonstrated that the colloidal silicic acid procedure yielded identical hemagglutination inhibition titers. In addition, it is technically feasible to read titers below 1:8.

scholarly journals Improved rubella hemagglutination inhibtion test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase C

1976 ◽  
Vol 4 (6) ◽  
pp. 461-466
Author(s):  
S Iwasa ◽  
M Hori
Keyword(s):  
Phospholipase C ◽  
Specific Antibody ◽  
Rubella Virus ◽  
Hemagglutination Inhibition ◽  
Dextran Sulfate ◽  
Enzymatic Action ◽  
Rubella Antibody

A method using phospholipase C (PL-C) for removing nonspecific inhibitors (NSI) of rubella virus hemagglutinin is described. PL-C was found to hydrolyze NSI without altering the hemagglutination inhibition (HI) activity of the specific antibody and could be used to remove NSI in the rubella HI test by using formalinized erythrocytes, which resisted the enzymatic action; fresh erythrocytes were lysed by PL-C. The HI test using PL-C treated sera gave true measurements of actual rubella antibody content, and HI titers of PL-C treated sera were identical or equivalent (+/-1 dilution) to those of sera treated with dextran sulfate and CaCl2 (DS-C). Thus, the PL-C method gave results as reproducible and reliable as the DS-C method and was more convenient.

Titania Nanotubes Prepared by Templating Needle-Like Calcium Carbonate

2006 ◽  
Vol 11-12 ◽  
pp. 539-542
Author(s):  
Li Dong Gao ◽  
Jian Feng Chen
Keyword(s):  
Calcium Carbonate ◽  
Heterogeneous Nucleation ◽  
Room Temperature ◽  
Simple Procedure ◽  
Hollow Structure ◽  
Titania Nanotubes ◽  
Bet Surface Area ◽  
Crystal Nucleus ◽  
Caco3 Nanoparticles ◽  
Hydrolysis Of

Titania nanotubes were successfully synthesized by a simple procedure with needle-like CaCO3 as inorganic templates at a room temperature in nonaqueous system. Through the hydrolysis of tetrabutoxytitanium (TBOT), titania crystal nucleus were deposited on the CaCO3 nanoparticles due to heterogeneous nucleation, followed by aggregation condensation on the surface of needle-like CaCO3 cores and removal of CaCO3 to produce hollow titania nanotubes. Its morphological and structural properties were characterized by TEM, SEM, and XRD, respectively. The nanotubes have a uniform tubular hollow structure with one or two big openings ends, the length of about 2.0 μm, the average inner diameters of 100-200 nm, respectively, and a wall thickness of approximately 40 nm. The phase formed was anatase after calcinations at 723 K for 2 h and nanotubes with hollow structure remained their original shapes, and the BET surface area of as-synthesized titania nanotubes was 243.45 m2/g, and decreased to 144.76 m2/g after calcinations

scholarly journals Regioselective Thiocyanation of Aromatic and Heteroaromatic Compounds Using [2-(Sulfooxy)ethyl]sulfamic Acid as an Efficient, Recyclable Organocatalyst and Novel Difunctional Brønsted Acid

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Sami Sajjadifar ◽  
Saaid Karimian ◽  
Hadi Noorizadeh ◽  
Hojat Veisi
Keyword(s):  
Hydrogen Peroxide ◽  
Aromatic Amines ◽  
Room Temperature ◽  
Simple Procedure ◽  
Sulfamic Acid ◽  
Chlorosulfonic Acid ◽  
Substituted Anilines ◽  
Heteroaromatic Compounds ◽  
Reaction Cycles ◽  
Considerable Loss

A green and simple procedure for the thiocyanation of aromatic and heteroaromatic compounds is described. [2-(Sulfooxy)ethyl]sulfamic acid (SESA) (supported on silica) is easily produced by addition of chlorosulfonic acid to 2-aminoethanol and this catalyst is applied as an efficient, reusable, and heterogeneous catalyst for the thiocyanation of heterocycles, substituted anilines (electron-rich and electron-deficient), and N,N-disubstituted aromatic amines using hydrogen peroxide in the water : ethanol as a solvent at room temperature. The catalyst can be easily recovered and reused for five reaction cycles without considerable loss of activity.

scholarly journals A Green, Expeditious, One-Pot Synthesis of 3, 4-Dihydropyrimidin-2(1H)-ones Using a Mixture of Phosphorus Pentoxide-Methanesulfonic Acid at Ambient Temperature

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Amulrao Borse ◽  
Mahesh Patil ◽  
Nilesh Patil ◽  
Rohan Shinde
Keyword(s):  
Reaction Time ◽  
Ambient Temperature ◽  
Room Temperature ◽  
Simple Procedure ◽  
Methanesulfonic Acid ◽  
Phosphorus Pentoxide ◽  
One Pot ◽  
Short Reaction Time ◽  
Solvent Free Conditions ◽  
High Yields

An expeditious, one-pot method for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones using a mixture of phosphorus pentoxide-methanesulfonic acid (Eaton’s reagent) at room temperature under solvent-free conditions is described. The salient features of this method include short reaction time, green aspects, high yields, and simple procedure.

Agents affecting lipid metabolism. XXXVII. Separation of rat serum lipoproteins with dextran sulfate

1969 ◽  
Vol 47 (11) ◽  
pp. 1043-1047 ◽  
Author(s):  
P. Hill ◽  
D. Dvornik
Keyword(s):  
Lipid Metabolism ◽  
Lipid Composition ◽  
Dextran Sulfate ◽  
Fundamental Difference ◽  
High Density ◽  
Low Density ◽  
Serum Lipoproteins ◽  
Rat Serum ◽  
Density Fractions ◽  
Estradiol 17Β

Dextran sulfate was used to separate rat serum lipoproteins into insoluble low-density and soluble high-density fractions. Lipid composition of the separated fractions was compared to that of lipoprotein fractions prepared by ultracentrifugation; data indicate that dextran sulfate precipitates lipoproteins with a density lower than 1.063.In rats treated with ethyl p-chlorophenoxyisobutyrate and estradiol-17β comparable, though not identical, changes in the composition of serum lipoproteins were observed with both methods of separation. The observed differences may reflect the fundamental difference in the basis of separation used by the methods. Data obtained with dextran sulfate may indicate changes in the protein moieties of lipoproteins.

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