Comparative Analysis of the Efficiency of the Binding of Phage Particles Displaying Tumor-Targeting Peptides to Cancer and Healthy Brain Cells

The binding efficiency of tumor-targeting phage particles, which exhibited tumor-addressing peptides and were obtained by phage display, to the human glioblastma cell line U-87 MG and health brain cells DKM- 5 has been evaluated by flow cytometry and enzyme-linked immunosorbent assay (ELISA). The specific binding of the selected bacteriophages to U-87 MG cells was shown by fluorescence microscopy. Based on the obtained data, tumor-targeting phage particles were selected that provide the most efficient binding to the U-87 MG cells in vitro for further studies in order to create targeted antitumor compounds. phage display, tumor-addressing peptides, glioblastoma, flow cytometry, ELISA The authors are grateful to S. I. Baiborodin, Head of the Microscopic Analysis of Biological Objects Core Facilities Center, SB RAS, for assistance in conducting confocal microscopy. This study was supported by the Russian Science Fund (grant no. 19-44-02006).

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COMPARATIVE ANALYSIS OF THE BINDING EFFICIENCY OF TUMOR- TARGETING PHAGE PARTICLES INTO CANCER CELLS AND HEALTH BRAIN CELLS

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES ◽

10.37747/2312-640x-2020-18-23-24 ◽

2020 ◽

pp. 23-24

Author(s):

A. Voitova ◽

M. Dmitrieva ◽

V. Richter ◽

E. Kuligina

Keyword(s):

Flow Cytometry ◽

Glioma Cell ◽

Tumor Targeting ◽

Glioma Cells ◽

Glioma Cell Line ◽

Enzyme Linked Immunosorbent Assay ◽

Brain Cells ◽

Human Glioma ◽

Human Glioma Cells

The binding efficiency of tumor-targeting phage particles, obtained by phage display, into human glioma cell line U-87 MG and health brain cells was evaluated by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Based on the obtained data, tumor-targeting phage particles that provide the most efficient binding to human glioma cells U-87 MG in vitro are selected for further studies.

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Review for "A comparative analysis of cone photoreceptor morphology in bowhead and beluga whales"

10.1002/cne.25101/v2/review2 ◽

2020 ◽

Keyword(s):

Comparative Analysis ◽

Cone Photoreceptor ◽

Beluga Whales

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Review for "A comparative analysis of cone photoreceptor morphology in bowhead and beluga whales"

10.1002/cne.25101/v2/review1 ◽

2020 ◽

Keyword(s):

Comparative Analysis ◽

Cone Photoreceptor ◽

Beluga Whales

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Review for "A comparative analysis of cone photoreceptor morphology in bowhead and beluga whales"

10.1002/cne.25101/v1/review1 ◽

2020 ◽

Keyword(s):

Comparative Analysis ◽

Cone Photoreceptor ◽

Beluga Whales

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Presence of antibody in virus-infected brain cells of SSPE ferrets

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077591 ◽

1983 ◽

Vol 41 ◽

pp. 804-805

Author(s):

Hannah R. Brown ◽

Tammy L. Donato ◽

Halldor Thormar

Keyword(s):

Measles Virus ◽

Plasma Cells ◽

Subacute Sclerosing Panencephalitis ◽

Protein A ◽

Neutralizing Antibody ◽

Brain Cells ◽

Antibody Titers ◽

A Cell ◽

The Central Nervous System ◽

The Brain

Measles virus specific immunoglobulin G (IgG) has been found in the brains of patients with subacute sclerosing panencephalitis (SSPE), a slowly progressing disease of the central nervous system (CNS) in children. IgG/albumin ratios indicate that the antibodies are synthesized within the CNS. Using the ferret as an animal model to study the disease, we have been attempting to localize the Ig's in the brains of animals inoculated with a cell associated strain of SSPE. In an earlier report, preliminary results using Protein A conjugated to horseradish peroxidase (PrAPx) (Dynatech Diagnostics Inc., South Windham, ME.) to detect antibodies revealed the presence of immunoglobulin mainly in antibody-producing plasma cells in inflammatory lesions and not in infected brain cells.In the present experiment we studied the brain of an SSPE ferret with neutralizing antibody titers of 1:1024 in serum and 1:512 in CSF at time of sacrifice 7 months after i.c. inoculation with SSPE measles virus-infected cells. The animal was perfused with saline and portions of the brain and spinal cord were immersed in periodate-lysine-paraformaldehyde (P-L-P) fixative. The ferret was not perfused with fixative because parts of the brain were used for virus isolation.

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