3D printing is used to produce individual objects or to print on different substrates to produce multi-component products. In the textile industry, we encounter various 3D printing technologies in fashion design, functional apparel manufacturing (protective, military, sports, etc.), including wearable electronics, where textile material is functionalized. 3D printing enables the personalization of the product, which in the apparel industry can be transformed into the production of clothing or parts of clothing or custom accessories. Additive technology allows a more rational use of the material than traditional technologies. In the textile industry we meet different uses of it, one is the printing of flexible structures based on rigid materials, another is the printing with flexible materials and the third is the printing directly on textile substrate. All rigid, hard and soft or flexible materials can be integrated into the final design using 3D printing directly on the textile substrate. We speak of so-called multi-material objects and systems, which have many advantages, mainly in the increasing customization and functionalization of textiles or clothing. The article gives a broader overview of 3D printing on textiles and focuses mainly on the influence of different parameters of printing and woven fabric properties on the adhesion of 3D printed objects on the textile substrate. In our research we investigated the influence of twill weave and its derivate as well as different weft densities of the woven fabric on the adhesion of printed objects on textile substrate. Therefore, five samples of twill polyester/cotton fabrics were woven and their physical properties measured for this research. 3D objects were printed on textile substrates using the extrusion based additive manufacturing technique with polylactic acid (PLA) filament. Preliminary tests were carried out to define printing parameters and different methods of attaching the fabric to a printing bed were tested. T - Peel adhesion tests were performed on the Instron dynamometer to measure the adhesion between 3D printed objects and textile substrates.