Subcutaneous fibrosarcomas with pulmonary metastases in a white tiger (Panthera tigris) and a lion (Panthera leo)

2020 ◽  
Vol 8 (2) ◽  
pp. e000960
Author(s):  
Deborah Johanna Eikelberg ◽  
Lisa Allnoch ◽  
Pierre Grothmann ◽  
Julia Bohner ◽  
Marion Hewicker-Trautwein
Keyword(s):  
Pulmonary Metastases ◽  
Smooth Muscle Actin ◽  
Growth Factor Receptor ◽  
Giant Cells ◽  
Panthera Tigris ◽  
Muscle Actin ◽  
Domestic Cats ◽  
Single Mass ◽  
White Tiger ◽  
Pulmonary Masses

Two cases of recurrent subcutaneous fibrosarcomas in a white tiger and a lion were observed and the animals were euthanised humanely due to clinical deterioration. In both animals, postmortem examination revealed multinodular, white to fawn, firm to greasy, subcutaneous masses at the left side of the thorax infiltrating into the adjacent musculature. Furthermore, the tiger showed a single mass and the lion multiple masses in the lung. Histopathologically, the subcutaneous and pulmonary masses consisted of spindle-shaped neoplastic cells with necrotic areas, and infiltration with multinucleated giant cells and lymphocytes. Immunohistochemically, tumour cells labelled positive for vimentin and negative for desmin, factor VIII-related antigen, smooth muscle actin S100, CD31 and nerve growth factor receptor p75. Thus, the pulmonary tumours were diagnosed as metastases of subcutaneous fibrosarcomas. Like domestic cats, also large, non-domestic felids could be predisposed for metastasising fibrosarcoma, which may be associated with injections or trauma.

scholarly journals Vaginal fibroleiomyoma in a cow: a case report

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 138-141 ◽  
Author(s):  
N. Timurkaan ◽  
M. Aydin ◽  
F. Yilmaz ◽  
A. Cevik
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Giant Cells ◽  
Vaginal Wall ◽  
Muscle Actin ◽  
Vaginal Route ◽  
Immunohistochemical Examination ◽  
Brown Swiss ◽  
Cd 68 ◽  
The Masses

: This paper describes a case of fibroleiomyoma seen in the vagina of a cow, diagnosed on the basis of macroscopic, microscopic and immunohistochemical findings. A five year-old female, Simmental and Brown Swiss crossbreed cow presented with six neoplastic masses located on the vaginal wall. The masses were surgically removed through the vaginal route and were firm and well demarcated. Microscopic examination showed that the non-encapsulated neoplastic nodules consisted of the admixture of smooth muscle and connective tissue. Immunohistochemical examination revealed strong focal positive reactions for smooth muscle actin and vimentin, but no positive reaction for CD 68. The tumour reported here was considered benign because of the lack of clear pleomorphism, invasivness, multinuclear giant cells and atypia, and low mitotic activity.

scholarly journals Is Pneumatosis Cystoides Intestinalis Gas-Distended and Ruptured Lymphatics?: Reappraisal by Immunohistochemistry

2014 ◽  
Vol 138 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Xianyong Gui ◽  
Yi Zhou ◽  
Leslie Eidus ◽  
Vincent Falck ◽  
Zu-hua Gao ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Linear Structure ◽  
Giant Cells ◽  
Pneumatosis Cystoides Intestinalis ◽  
Muscle Actin ◽  
Lymphatic Endothelial Cells ◽  
Diverse Background ◽  
The Right ◽  
Age Range

Context.—Pneumatosis cystoides intestinalis (PCI) is a condition with multiple gas-filled cysts within the bowel wall, associated with diverse background diseases. Its pathogenesis is still a mystery. Some previous observations scattered in the literature have suggested an association of the cystic spaces in PCI with the lymphatics. Objective.—To further investigate whether PCI results from the ballooning of gas-filled lymphatic channels. Design.—We did immunostaining of podoplanin, a mucoprotein preferentially expressed in lymphatic endothelial cells, in 13 cases (8 men, 5 women; age range, 18–80 years) of PCI. Ten cases were diagnosed in resected segments of bowel and 3 in biopsies. Pneumatosis was seen in the right side of the colon (9 cases), transverse colon (1 case), sigmoid colon (1 case), and small bowel (2 cases). In addition, immunostaining for CD31, calretinin, WT1, CD68, smooth muscle actin, desmin, vimentin, and cytokeratins was also performed for comparison and correlation. Results.—A strong immunopositivity of podoplanin was seen in a condensed linear structure in the pericystic interstitium in 100% of the cases, but was not seen in the overlying giant and flat cells that were all CD68-positive histiocytes. Meanwhile, the podoplanin-expressing structure was negative for calretinin and WT1, which ruled out the possible mesothelial origin. There were coexistent variable immunopositivity of smooth muscle actin, which suggests an admixture of myofibroblasts. These findings indicated that the PCI cases were gas-distended lymphatics with the lymphatic epithelium ruptured and embedded in the reactive histiocytes and giant cells. Conclusion.—Our findings support the lymphatic theory about the pathogenesis of PCI.

Primary Prostatic Leiomyosarcoma With Pulmonary Metastases in a Dog

2010 ◽  
Vol 46 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Barbara Bacci ◽  
Massimo Vignoli ◽  
Federica Rossi ◽  
Francesco Gallorini ◽  
Rossella Terragni ◽  
...  
Keyword(s):  
Immunohistochemical Staining ◽  
Pulmonary Metastases ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Computed Tomography Scan ◽  
Intact Male ◽  
Spindle Cells ◽  
S 100 ◽  
Total Body ◽  
Tomography Scan

A 6-year-old, intact male Jack Russell terrier was diagnosed with a mass in the caudal abdomen, and ultrasound revealed a large prostatic mass. A total-body computed tomography scan was performed for staging, and lung nodules were detected. Histological examination showed a proliferation of spindle cells arranged in interlacing fascicles. Immunohistochemical staining revealed cells were cytokeratin negative and immunoreactive for vimentin and α-smooth muscle actin; cells stained with desmin and S-100 were negative. A diagnosis of primary prostatic leiomyosarcoma with pulmonary metastases was made. This is one of the rare cases of primary prostatic mesenchymal tumor in the canine species.

scholarly journals Omental leiomyosarcoma with unusual giant cells in a Beagle dog — Short communication

2016 ◽  
Vol 64 (2) ◽  
pp. 222-228
Author(s):  
Jun Sasaki ◽  
Megumi Toyoshima ◽  
Yasuhiko Okamura ◽  
Masanobu Goryo
Keyword(s):  
Computed Tomography ◽  
Abdominal Pain ◽  
Short Communication ◽  
Smooth Muscle Actin ◽  
Abdominal Cavity ◽  
Giant Cells ◽  
Greater Omentum ◽  
Muscle Actin ◽  
Beagle Dog ◽  
History Of

A 10-year-old castrated male Beagle dog was presented with a 2-month history of intermittent vomiting and abdominal pain. The dog was referred to the Veterinary Teaching Hospital at Iwate University for further evaluation, and a splenic tumour was suspected on the basis of ultrasonography and computed tomography. Surgery identified a large, solid, light-pink mass on the greater omentum with blood-coloured ascites in the abdominal cavity, and resection was performed. Microscopically, the mass comprised spindle-shaped tumour cells and scattered osteoclast-like giant cells. Most spindle-shaped cells were positive for vimentin, desmin, and smooth muscle actin (α-SMA), whereas osteoclast-like giant cells were positive only for vimentin. On the basis of histopathological and immunohistochemical findings, a diagnosis of leiomyosarcoma was made. To the best of our knowledge, this represents the first report of leiomyosarcoma associated with osteoclast-like giant cells developing from the greater omentum in a dog.

scholarly journals Pulmonary vascular proliferation in patients with severe COVID-19: an autopsy study

Thorax ◽  
2021 ◽  
pp. thoraxjnl-2020-216714
Author(s):  
Belén Pérez-Mies ◽  
María Gómez-Rojo ◽  
Irene Carretero-Barrio ◽  
Tommaso Bardi ◽  
Amparo Benito ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Diffuse Alveolar Damage ◽  
Smooth Muscle Actin ◽  
Growth Factor Receptor ◽  
Platelet Derived Growth Factor ◽  
Vascular Endothelial ◽  
Muscle Actin ◽  
Autopsy Study ◽  
Vascular Endothelial Cadherin ◽  
Vascular Proliferation

Diffuse alveolar damage and thrombi are the most common lung histopathological lesions reported in patients with severe COVID-19. Although some studies have suggested increased pulmonary angiogenesis, the presence of vascular proliferation in COVID-19 lungs has not been well characterised. Glomeruloid-like microscopic foci and/or coalescent vascular proliferations measuring up to 2 cm were present in the lung of 14 out of 16 autopsied patients. These lesions expressed CD31, CD34 and vascular endothelial cadherin. Platelet-derived growth factor receptor-β immunohistochemistry and dual immunostaining for CD34/smooth muscle actin demonstrated the presence of pericytes. These vascular alterations may contribute to the severe and refractory hypoxaemia that is common in patients with severe COVID-19.

scholarly journals Lung Metastasis after an Eighteen-Years-Long Disease-Free Period since Uterine Leiomyosarcoma Diagnosis

2014 ◽  
Vol 2014 ◽  
pp. 1-3
Author(s):  
M. Guazzaroni ◽  
D. Tosti ◽  
M. Rascioni ◽  
M. Mataloni ◽  
D. Citraro ◽  
...  
Keyword(s):  
Lung Metastasis ◽  
Pulmonary Metastases ◽  
Smooth Muscle Actin ◽  
Metastatic Potential ◽  
Uterine Leiomyosarcoma ◽  
Muscle Actin ◽  
Uterine Sarcomas ◽  
Disease Free Interval ◽  
Free Interval ◽  
Disease Free

Uterine leiomyosarcoma (ULMS) is an uncommon malignancy that accounts for one-third of uterine sarcomas and represents 1% of all uterine malignancies, with an incidence averaging 0.5–1/100,000/year. The prognosis is poor due to its intrinsic aggressiveness and its characteristic high metastatic potential with reported distant metastatic spread in lung, abdomen, soft tissue, and brain. We present the case of a 67-year-old woman with lung metastasis after eighteen years since uterine leiomyosarcoma diagnosis and its following surgical resection. The diagnosis of pulmonary metastases was obtained by reviewing the histology of the previous uterine tumor: the tumor cells were immunoreactive for CD10, PR, and smooth muscle actin (SMA), but negative for desmin, S100, CD34, CD 117, cytokeratins AE1AE3, CD68R, and ER. To our knowledge, this disease-free interval is the longest among previous reports of pulmonary metastasis of uterine leiomyosarcoma.

scholarly journals Fatal Cytauxzoonosis in a Captive-reared White Tiger (Panthera tigris)

1996 ◽  
Vol 33 (1) ◽  
pp. 82-86 ◽  
Author(s):  
M. M. Garner ◽  
N. P. Lung ◽  
S. Citino ◽  
E. C. Greiner ◽  
J. W. Harvey ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Severe Thrombocytopenia ◽  
Panthera Tigris ◽  
Domestic Cats ◽  
Mild Anemia ◽  
Large Numbers ◽  
Cytauxzoon Felis ◽  
History Of ◽  
White Tiger ◽  
Histologic Changes

Fatal (Panthera tigris) cytauxzoonosis was diagnosed in a 7-year-old female white tiger. The tiger presented with a 2-day history of anorexia and lethargy. She was mildly dehydrated, with a temperature of 105.2 F and a hematocrit of 26%. Over the next day, icterus developed, and her physical condition progressed to recumbency, coma, and death. Hematologic findings obtained shortly before death included icteric plasma, severe thrombocytopenia, mild anemia, hematuria, and parasites consistent with Cytauxzoon felis in circulating erythrocytes. Gross necropsy findings included generalized icterus, generalized petechiae and ecchymoses, splenomegaly, and peribronchial edema. Histologic changes included large numbers of intravascular macrophages containing developmental stages of Cytauxzoon felis that partially or completely occluded blood vessels in the lung, spleen, liver, and bone marrow. Except for an experimental infection of a bobcat, fatal cytauxzoonosis has not previously been diagnosed in felids other than domestic cats. These findings raise questions regarding the pathogenicity of this organism in felids and may impact husbandry and interstate transfer of captive large cats.

Feline Intraocular Tumors May Arise from Transformation of Lens Epithelium

2003 ◽  
Vol 40 (4) ◽  
pp. 355-362 ◽  
Author(s):  
C. J. Zeiss ◽  
E. M. Johnson ◽  
R. R. Dubielzig
Keyword(s):  
Smooth Muscle ◽  
Basement Membrane ◽  
Collagen Type ◽  
Smooth Muscle Actin ◽  
Growth Factor Receptor ◽  
Collagen Type Iv ◽  
Muscle Actin ◽  
Cell Of Origin ◽  
Type Iv ◽  
Membrane Type

Feline ocular sarcomas are malignant intraocular neoplasms that are frequently associated with a history of ocular trauma. They usually present as fibrosarcomas, but some have both epithelial and mesenchymal features. The purpose of this study was to determine the cell of origin of a subset of feline intraocular sarcomas that display a mixed epithelial-mesenchymal phenotype, with elaboration of basement membrane—type matrix. We examined the morphology and histochemical and immunohistochemical phenotypes of nine feline intraocular sarcomas. Immunohistochemistry and in situ hybridization were performed to detect expression of crystallin alpha A. In addition, tumors were examined for expression of vimentin, cytokeratin, smooth muscle actin, desmin, melan A, neural cell adhesion molecule, S-100, glial fibrillary acidic protein, nerve growth factor receptor, and collagen type IV. Animals ranged from 7 to 17 years of age—no breed or sex predilection for tumor occurrence was present. Tumors were characterized by mixed epithelial and mesenchymal phenotypes, both of which elaborated basement membrane-type material and expressed vimentin highly. On the basis of collagen type IV and crystallin alpha A immunopositivity, we established that three of nine tumors were of lens epithelial origin. Expression of desmin and smooth muscle actin identified one tumor as a leiomyosarcoma. The remainder were undifferentiated sarcomas of myofibroblastic origin. This is the first report of lens epithelial neoplasia in clinical material from any species. The history and morphologic features of feline ocular sarcomas are reminiscent of feline vaccine-induced sarcomas. These tumors may share pathophysiologic similarities unique to this species.

scholarly journals Piloleiomyosarcoma in cats: Histological and immunohistochemical features

2021 ◽  
pp. 030098582110425
Author(s):  
Francisco Rodríguez Guisado ◽  
Pedro Luis Castro
Keyword(s):  
Smooth Muscle ◽  
Subcutaneous Tissue ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Giant Cells ◽  
Hair Follicles ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Neoplastic cells had eosinophilic and fibrillar cytoplasm, and elongated blunt-ended nuclei. Entrapment of hair follicles and absence of vascular components support an origin from the smooth muscle cells of the arrector pili. Additional findings included bizarre nuclei and giant cells (7/9 cases), atypical mitoses (7/9 cases), ulceration (3/9 cases), and intratumoral necrosis (6/9 cases). Neoplastic cells expressed calponin, desmin, α-smooth muscle actin, and vimentin, but not CD18, CD31, cytokeratins, glial fibrillary acidic protein, neuron-specific enolase, Melan A, p63, or S-100 protein. Surgical excision was curative in 6/9 cases, with local recurrence in 2/9 cases and metastasis to local lymph nodes in 1/9 case. Clinical outcome was influenced by mitotic count, infiltration of subcutaneous tissue, and intensity of nuclear immunolabeling for p53.

Epithelioid Leiomyosarcoma With Osteoclast-like Giant Cells in the Rectum

2000 ◽  
Vol 124 (3) ◽  
pp. 438-440 ◽  
Author(s):  
Tadashi Terada ◽  
Kanenori Endo ◽  
Hiroyuki Maeta ◽  
Satoshi Horie ◽  
Tetsuo Ohta
Keyword(s):  
Tumor Cells ◽  
Biopsy Specimen ◽  
Smooth Muscle Actin ◽  
Cell Types ◽  
Giant Cells ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
First Case ◽  
Old Japanese ◽  
Giant Tumor

Abstract We report a rare case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells. A 71-year-old Japanese man was admitted to a hospital with melena. Results of a colonoscopy test revealed a polypoid tumor in the rectum, and a biopsy specimen from the lesion showed a sarcoma; the patient underwent rectosigmoidectomy. At gross inspection, the tumor measured 8 × 7 × 4 cm and was polypoid with ulcerations. Necrotic and hemorrhagic foci were scattered. Microscopically, the tumor consisted of 2 cell types: malignant tumor cells with epithelioid features and benign-appearing osteoclast-like giant cells. The tumor cells were polygonal and epithelioid in shape and had eosinophilic or clear cytoplasms, with scattered giant tumor cells. Immunohistochemical examination revealed that the tumor cells were positive for vimentin, muscle actin, α-smooth muscle actin, and desmin, whereas the osteoclast-like giant cells were positive for CD68, leukocyte common antigen, and lysozymes. We diagnosed this case as epithelioid leiomyosarcoma with osteoclast-like giant cells. To the best of our knowledge, this is the first case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells.

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