Production of perithecia by isolates of Gnomonia fructicola

1972 ◽  
Vol 50 (1) ◽  
pp. 49-51
Author(s):  
C. O. Gourley
Keyword(s):  
Potato Dextrose Agar ◽  
Agar Media ◽  
Cornmeal Agar

More isolates of Gnomonia fructicola formed perithecia on Leonian's agar than on potato-dextrose or cornmeal agar. Fortifying these media with a strawberry petiole extract stimulated perithecium formation on potato-dextrose agar but not on Leonian's or cornmeal agar. All eight isolates studied produced perithecia on strawberry petioles and one produced them only on petioles. The eight isolates segregated into four cultural races according to their ability to form perithecia on the agar media and petioles. This explains why different media have been reported to be best for perithecium production in this fungus.

scholarly journals Occurrence of Fusarium species in maize grains for silage

2011 ◽  
Vol 27 (3) ◽  
pp. 1235-1240
Author(s):  
V. Krnjaja ◽  
J. Levic ◽  
S. Stankovic ◽  
Z. Bijelic ◽  
V. Mandic ◽  
...  
Keyword(s):  
Moisture Content ◽  
Fusarium Species ◽  
Potato Dextrose Agar ◽  
Nutrient Agar ◽  
Maize Hybrid ◽  
Maize Hybrids ◽  
Genus Fusarium ◽  
Agar Media ◽  
Late Maturity

Grain samples of two maize hybrids, medium early (ZP434) and late maturity (ZP704), collected during harvest in 2008 were investigated for contamination by fungi. Grains were plated on agar media and grown fungi were identified by morphological macroscopic and microscopic characteristics on potato-dextrose agar (PDA) and synthetic nutrient agar (SNA). Species of the genus Fusarium were the most common in both hybrids, and their presence amounted to 33.89% (ZP434) and 42% (ZP704). Other fungi of genera, Acremonium, Alternaria, Aspergillus, Chaetomium, Cladosporium, Nigrospora, Penicillium, Rhizopus and Trichotecium, were isolated from 0 to 41%. Four species belonging to the genus Fusarium were identified, of which the species F. verticillioides was the most common with 28.63% in ZP434 and 30.5% in ZP704 hybrids. The presence of F. graminearum, F. proliferatum and F. subglutinans ranged from 3% (ZP704) to 5% (ZP434), 0.13% (ZP434) to 7% (ZP704) and 0.13% (ZP434) to 7% (ZP704), respectively. Generally, the incidence of every particular fungus was higher in the late maize hybrid with a higher moisture content than in the medium-early hybrid with a lower moisture content.

scholarly journals Evaluation of Bioagents and Biofertilizers for the Managament of Seed and Seedling Diseases of Sesamum indicum (Sesame)

2013 ◽  
Vol 2 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Venturla Bharathi ◽  
Ravuru Sudhakar ◽  
K. Parimala ◽  
Vishnuvardhan A. Reddy
Keyword(s):  
Andhra Pradesh ◽  
Seed Quality ◽  
Pathogenic Fungi ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Sesamum Indicum ◽  
Potato Dextrose Agar ◽  
Seedling Mortality ◽  
Sesame Seeds ◽  
Agar Media

The study was carried out to evaluate the response of biopeticides and biofertilizers on seed mycoflora and seed quality parameters of Sesame (Sesamum  indicum  L.). Untreated Sesame seeds were collected from farmers of Nizamabad and Karimanagar districts of Andhra Pradesh in India and discolored seeds were separated and treated with biofertilizers and biopesticides alone and in combination form. The seed mycoflora of Sesame seeds were screened by using Potato dextrose agar (PDA) medium and czaepek dox agar media. The results indicate that maximum numbers of fungi were recorded on PDA. The untreated seeds were found to be associated with maximum percent incidence of mycoflora and minimum population was recorded in the treatment of Trichoderma + Pseudomonas formulation followed by Azat obacter + Trichoderma, Pseudomonas and Azatobacter in the decreasing order of efficacy. This study also showed relation of biofertilizers and biopesticides and seed mycoflora on seed germination. Germination percentage was maximum in the treatment Trichoderma + Pseudomonas formulation, Azatobacter + Trichoderma, Pseudomonas and Azatobacter recording 96%, 94%, 90% and 88%, respectively. In the control, germination percentage was minimum compared with other treatments. Seeds treated with the mixed formulation were found beneficial in reducing the pathogenic fungi and decreasing seedling mortality.

scholarly journals Microbial Isolation from Gastrointestinal Tract of Catfish (Clarias gariepinus)

2020 ◽  
Vol 2 (1) ◽  
pp. 22-30
Author(s):  
Nurul Aini
Keyword(s):  
Gastrointestinal Tract ◽  
Science And Technology ◽  
Clarias Gariepinus ◽  
Potato Dextrose Agar ◽  
Nutrient Agar ◽  
Microbiology Laboratory ◽  
Microbial Isolation ◽  
Agar Media ◽  
De Man ◽  
Number Of Bacteria

The purpose of this research was to describe the characteristics and number of microbes that grow in the gastrointestinal tract of catfish and to determine the potential of microbes as probiotics. The type of this research was observational conducted on 30 September-15 October 2018 at the Microbiology Laboratory, Faculty of Science and Technology, Universitas Airlangga, Surabaya. Microbial isolation using NA (Nutrient Agar), MRSA (de Man, Rogosa and Sharpe Agar) and PDA (Potato Dextrose Agar) media. The fish that used is catfish that are bred in ponds at Desa Tlasih, Kecamatan Tulangan, Kabupaten Sidoarjo, East Java. The identification includes microscopic and macroscopic characteristics. Based on the research that has been done, it can be concluded that there are 8 kinds of bacterial colonies in NA media, on PDA media there are 8 kinds of fungi, and on MRSA media there are 2 bacterial colonies and each has different macroscopic and microscopic characteristics. The total number of bacteria growing in NA and MRSA media respectively were 8.7 x 104 CFU / gram and 1.2 x 105 CFU / gram. It is suspected that there are potential bacteria as beneficial probiotics for catfish which still need further research. Keywords: catfish, gastrointenstinal tract, microbes, bacteria, fungi

scholarly journals Alternative medium for the growth of endophytic fungi

2021 ◽  
Vol 886 (1) ◽  
pp. 012045
Author(s):  
Syamsia Syamsia ◽  
Abubakar Idhan ◽  
Husnah Latifah ◽  
Noerfitryani Noerfityani ◽  
Aidil Akbar
Keyword(s):  
Organic Matter ◽  
Research Methods ◽  
Growth Medium ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Organic Medium ◽  
Potato Dextrose Agar ◽  
Organic Media ◽  
Alternative Ingredients ◽  
Agar Media

Abstract Medium for the growth of endophytic fungi generally uses Potato Dextrose Agar media, but because the price is expensive, it is necessary to find materials for alternative media from organic materials that are easy to obtain and inexpensive. The legume group was one of the alternative ingredients as the source of protein, corn, and rice as the source of carbohydrates for the growth medium. This study aimed to determine the potential of organic matter such as rice, corn, and legumes as a medium for the growth of endophytic fungi. The research methods included: rejuvenation of endophytic fungus isolation, preparation of organic medium from rice, corn, legumes, and potatoes, growth test of endophytic fungus on 4 types of organic media.

A 3-Vinyl Cephem Derivative, a Useful Intermediate in the Synthesis of Cepham Antibiotics, from Aspergillus awamori Associated with Banana Fruit

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
H.M.S.K.H Bandara ◽  
N. Savitri Kumar ◽  
Lalith Jayasinghe ◽  
Hironori Masubuti ◽  
Yoshinori Fujimoto
Keyword(s):  
Chemical Synthesis ◽  
Chromatographic Separation ◽  
Musa Acuminata ◽  
Potato Dextrose Agar ◽  
Natural Source ◽  
Aspergillus Awamori ◽  
Banana Fruit ◽  
Potato Dextrose Broth ◽  
Agar Media ◽  
First Time

Aspergillus awamori was isolated from a diseased banana fruit, Musa acuminata cv. Ambul. The fungus was fermented in potato dextrose broth and on potato dextrose agar media and the fungal media were extracted with EtOAc. Chromatographic separation of the EtOAc extracts furnished 4-methoxybenzyl 7-phenylacetamido-3-vinyl-3-cephem-4-carboxylate (1), along with three naphtho-γ-pyrones, flavasperone (2), foncesinone A (3) and aurasperone A (4), and three alkaloids, aspernigrin A (5), pestalamide C (6) and nigragillin (7). Compound 1, a known key intermediate in the chemical synthesis of cepham antibiotics, was isolated from a natural source for the first time. Compound 1 is the first 3-vinyl cephem derivative of microbial origin.

COMPARISON OF MEDIA FOR THE ISOLATION OF FUSARIUM OXYSPORUM F. SP. LYCOPERSICI FROM SAWDUST USED FOR GROWING TOMATOES

1974 ◽  
Vol 54 (2) ◽  
pp. 373-374
Author(s):  
M. G. ANDERSON ◽  
R. G. ATKINSON
Keyword(s):  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Rose Bengal ◽  
Antimicrobial Agents ◽  
Potato Dextrose Agar ◽  
Selective Isolation ◽  
Streptomycin Sulphate ◽  
Lycopersicon Esculentum Mill ◽  
Agar Media ◽  
Greenhouse Tomatoes

Various agar media amended with antimicrobial agents were compared for the selective isolation of Fusarium oxysporum f.sp. lycopersici from sawdust used for growing greenhouse tomatoes (Lycopersicon esculentum Mill.). Potato dextrose agar amended with pentachloronitrobenzene (PCNB) (500 mg/l) and streptomycin sulphate (300 mg/l proved to be the most satisfactory medium, tested. These antimicrobial agents, unlike rose bengal, did not reduce the number of Fusarium colonies isolated.

Tahap Kontaminasi Bioearosol: Satu Kajian Kes

2012 ◽  
Author(s):  
J. Norafiqah ◽  
M. Ahmad ◽  
R. Firdausi
Keyword(s):  
Sampling Technique ◽  
Potato Dextrose Agar ◽  
Nutrient Agar ◽  
Malt Extract Agar ◽  
Sampling Location ◽  
Contamination Level ◽  
Malt Extract ◽  
Extract Agar ◽  
Agar Media ◽  
Sampling Duration

Satu kajian menilai tahap kepekatan bioaerosol di ruangan pejabat telah dijalankan dengan menggunakan teknik persampelan hentaman. Dua pemboleh ubah utama, iaitu kadar alir udara, dan masa persampelan yang optimum ditentukan terlebih dahulu. Kesesuaian pertumbuhan bioaerosol khususnya bakteria dan fungus, juga diuji dengan menggunakan empat jenis agar iaitu nutrient agar, tryptic soy agar, potato dextrose agar dan malt extract agar. Keputusan mendapati bahawa kadar alir persampelan 28 liter per minit bersama masa persampelan selama enam minit dan penggunaan medium tryptic soy agar memberikan keputusan yang terbaik. Hasil kajian menunjukkan korelasi yang amat baik di antara tahap kontaminasi bioaerosol dan bilangan manusia yang terdapat di ruangan pejabat, dengan pekali korelasinya yang amat bererti iaitu r2 = 0.99. Kata kunci: Kualiti udara dalaman, pencemaran udara dalaman, bioaerosol A study to evaluate the level of bioaerosol contamination in the office spaces was performed using the impaction sampling technique. Two main variables, i.e. sampling air flow rates and duration as well as the suitability of four agar media i.e. nutrient agar, tryptic soy agar, potato dextrose agar and malt extract agar were predetermined. The data showed that the sampling flow rate of 28 Lpm with a sampling duration of 6 minutes applied on tryptic soy agar presented the best results. The study also revealed that there was a strong correlation (i.e. r2 = 0.99) between bioaerosol contamination level and the number of person in the given sampling location. Key words: Internal air quality, internal air pollution, bioaerosol

PHIALOPHORA RADICICOLA CAIN, A CORN ROOTROT PATHOGEN

1952 ◽  
Vol 30 (3) ◽  
pp. 344-347 ◽  
Author(s):  
W. E. McKeen
Keyword(s):  
Rhizoctonia Solani ◽  
Potato Dextrose Agar ◽  
Root Tissue ◽  
Sexual Stage ◽  
Corn Roots ◽  
Infected Root ◽  
Infection Threads ◽  
Agar Media ◽  
Host Relations ◽  
Apparently Healthy

During the summer of 1950, Phialophora radicicola, was found on corn roots in soil near Chatham, Ridgetown, and Harrow, Ont. Owing to its color and parasite-host relations, it may have been mistaken previously for Rhizoctonia solani or a Rhizophagus species. It can be isolated only when a piece of apparently healthy corn root tissue with the adhering mycelium is removed from an infected root and placed on agar media. After two or three months of culture on nutrient and on potato dextrose agar, P. radicicola lost its ability to sporulate, but this ability was revived when it was cultured on moist corn roots. No sexual stage of the organism has been found. The fungus may attack corn roots at any time throughout the season and its brown runner-hyphae or macrohyphae grow parallel to the roots. The finer infection hyphae or microhyphae penetrate the outer root tissue and their infection threads are surrounded by "wall tubules". The fungus is quite aggressive, but not very pathogenic, and apparently it is followed by numerous secondary organisms. The similarity of this organism to Ophiobolus graminis is very noticeable.

scholarly journals Sugarcane Bagasse: A Potential Medium for Fungal Cultures

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Arushdeep Sidana ◽  
Umar Farooq
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Energy Source ◽  
Sugarcane Bagasse ◽  
Low Cost ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Commercial Media ◽  
Residual Material ◽  
Unidentified Species ◽  
Cornmeal Agar

Worldwide, sugarcane industries produce tons of sugarcane bagasse as residual/waste material. This residual material is rich in complex lignocellulosic substances and may be used as a low cost carbon and energy source for the growth of fungal species. The present work was aimed at designing a sugarcane waste-based medium as a substitute for expensive commercial media for growing fungal cultures. Eight species of fungi, namely, Aspergillus niger, Candida albicans, Saccharomyces cerevisiae, Fusarium sp., and four unidentified species F1, F2, F3, and F5, were grown on the sugarcane bagasse medium which produced remarkable results and competed with standard media like potato dextrose agar, Sabouraud dextrose agar, and cornmeal agar. The designed medium was able to provide nourishment to the fungi as well as prevent the growth of any bacterial or fungal contaminant. The production of spores was more in the sugarcane medium as compared with standard media. Hence, this study led to the discovery of a new and efficient medium for fungal cultures as well as decrease in the waste disposal expenses and efforts.

scholarly journals Potential of endophytic fungi as biocontrol agents of Duponchelia fovealis (Zeller) (Lepidoptera:Crambidae)

2017 ◽  
Vol 78 (3) ◽  
pp. 429-435 ◽  
Author(s):  
R. F. Amatuzzi ◽  
N. Cardoso ◽  
A. S. Poltronieri ◽  
C. G. Poitevin ◽  
P. Dalzoto ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Mortality Rates ◽  
Potato Dextrose Agar ◽  
Biocontrol Agents ◽  
Commercial Farms ◽  
Agar Media ◽  
Petri Dishes ◽  
New Pest ◽  
Selection Of ◽  
Third Instar Larvae

Abstract This study reports the first assessment of endophytic fungi isolated from strawberry leaves and selection of isolates for the control of Duponchelia fovealis, a new pest of strawberries. A total of 400 strawberry leaves of the cultivar ‘Albion’ were collected in four commercial farms. Leaves were disinfected, cut in fragments, and placed on Petri dishes containing potato dextrose agar media with tetracycline and incubated for 30 days. Following this time, 517 fungal colonies were isolated, and thirteen genera were identified: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe, and Phoma. Eight isolates belonging to the genera Aspergillus, Diaporthe, Paecilomyces, and Cladosporium were selected for pathogenicity bioassays against third instar larvae of D. fovealis. Isolates of Paecilomyces induced the highest mortality rates.

Sign in / Sign up

Export Citation Format

Share Document