scholarly journals Sugarcane Bagasse: A Potential Medium for Fungal Cultures

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Arushdeep Sidana ◽  
Umar Farooq
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Energy Source ◽  
Sugarcane Bagasse ◽  
Low Cost ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Commercial Media ◽  
Residual Material ◽  
Unidentified Species ◽  
Cornmeal Agar

Worldwide, sugarcane industries produce tons of sugarcane bagasse as residual/waste material. This residual material is rich in complex lignocellulosic substances and may be used as a low cost carbon and energy source for the growth of fungal species. The present work was aimed at designing a sugarcane waste-based medium as a substitute for expensive commercial media for growing fungal cultures. Eight species of fungi, namely, Aspergillus niger, Candida albicans, Saccharomyces cerevisiae, Fusarium sp., and four unidentified species F1, F2, F3, and F5, were grown on the sugarcane bagasse medium which produced remarkable results and competed with standard media like potato dextrose agar, Sabouraud dextrose agar, and cornmeal agar. The designed medium was able to provide nourishment to the fungi as well as prevent the growth of any bacterial or fungal contaminant. The production of spores was more in the sugarcane medium as compared with standard media. Hence, this study led to the discovery of a new and efficient medium for fungal cultures as well as decrease in the waste disposal expenses and efforts.

scholarly journals Burkholderia thailandensis E264 as a promising safe rhamnolipids’ producer towards a sustainable valorization of grape marcs and olive mill pomace

2021 ◽  
Author(s):  
Alif Chebbi ◽  
Massimiliano Tazzari ◽  
Cristiana Rizzi ◽  
Franco Hernan Gomez Tovar ◽  
Sara Villa ◽  
...  
Keyword(s):  
Energy Source ◽  
Olive Oil ◽  
Low Cost ◽  
Carbon Sources ◽  
Burkholderia Thailandensis ◽  
Rhamnolipid Production ◽  
Key Points ◽  
Wide Range ◽  
Long Chain ◽  
Olive Mill

Abstract Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. Key points • Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). • Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. • Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities

Screening and Identification of Laccase Producing Fungi from Environmental Samples

2021 ◽  
Vol 6 (1) ◽  
pp. 91-98
Author(s):  
A. Bello ◽  
◽  
J. B. Ameh ◽  
D. A. Machido ◽  
A. I. Mohammed-Dabo
Keyword(s):  
Tannic Acid ◽  
Fungal Species ◽  
Soil Samples ◽  
Potato Dextrose Agar ◽  
Laccase Production ◽  
Curvularia Lunata ◽  
Main Campus ◽  
Fungal Isolates ◽  
Screening And Identification ◽  
Decaying Wood

Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and non-phenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from environment samples. Soil and decaying wood samples were collected from different locations within Ahmadu Bello University, Zaria Main campus. Suspensions of the samples (1 g in 10 mL sterile distilled water) were serially diluted, inoculated onto Potato Dextrose Agar (PDA) containing 0.01% Chloramphenicol and incubated for 7 days at 30oC.The fungal isolates were characterized macroscopically and microscopically with the aid of an atlas. The identified fungal isolates were screened for laccase production by inoculating onto PDA containing 0.02% Guaiacol, 1mM ABTS (2 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 0.5% Tannic acid as indicator compounds and incubated at 250C for 7 days. The laccase producing isolates were confirmed molecularly by ITS rDNA sequence analysis using the FASTA algorithm with the Fungus database from the European Bioinformatics Institute (EBI).A total of 25 fungal species (11 from soil and 14 from decaying wood samples) were isolated. Two isolates from the soil origin identified as Curvularia lunata SSI7 (Accession No. QIE06317.1) and Fusarium clade VII SSI3 (Accession No. GQ505677) were found to produce laccase where Curvularia lunata SSI7 was able to oxidize all the indicator compounds used for the screening. Fusarium clade VII SSI3 was able to oxidize only 0.5% Tannic acid. Laccase producing Curvularia lunata and Fusarium clade VII were isolated from soil samples collected from ABU Zaria Main Campus. Keywords: laccase, fungi, soil, decaying wood

scholarly journals Processing of porcelain stoneware tile using sugarcane bagasse ash waste

2015 ◽  
Vol 9 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Myrian Schettino ◽  
José Holanda
Keyword(s):  
Sugarcane Bagasse ◽  
Low Cost ◽  
Linear Shrinkage ◽  
Raw Material ◽  
Partial Replacement ◽  
Firing Cycle ◽  
Sintering Process ◽  
Natural Quartz ◽  
Sugarcane Bagasse Ash ◽  
Fast Firing

Large amounts of waste materials are discarded in the sugarcane industry. This work investigates the reuse of sugarcane bagasse ash waste as an alternative raw material for porcelain stoneware tile bodies, replacing natural quartz by up to 5 wt.%. The tile pieces were fired at 1230 ?C using a fast-firing cycle (< 60min). The technological properties of the fired tile pieces (e.g., linear shrinkage, water absorption, apparent density, and flexural strength) were determined. The sintering process was followed by SEM and XRD analyses. The results show that up to 2.5 wt.% sugarcane bagasse ash waste can be used as a partial replacement for quartz in porcelain stoneware tile (group BIa, ISO 13006 standard), providing excellent technical properties. Hence, its application in high-quality ceramic tile for use in civil construction as a low-cost, alternative raw material could be an ideal means of managing sugarcane bagasse ash waste.

scholarly journals Divergence of Eukaryotic Secretory Components: the Candida albicans Homolog of the Saccharomyces cerevisiae Sec20 Protein Is N Terminally Truncated, and Its Levels Determine Antifungal Drug Resistance and Growth

2001 ◽  
Vol 183 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Yvonne Weber ◽  
Uwe J. Santore ◽  
Joachim F. Ernst ◽  
Rolf K. Swoboda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Transcriptional Control ◽  
Secretory Pathway ◽  
Sodium Dodecyl ◽  
Fungal Species ◽  
Gene Encoding ◽  
Vesicle Traffic ◽  
Antifungal Drug Resistance ◽  
And Function

ABSTRACT Sec20p is a component of the yeast Saccharomyces cerevisiae secretory pathway that does not have a close homolog in higher eukaryotic cells. To verify the function of Sec20p in other fungal species, we characterized the gene encoding a Sec20p homolog in the human fungal pathogen Candida albicans. The deduced protein has 27% identity with, but is missing about 100 N-terminal residues compared to S. cerevisiae Sec20p, which is part of the cytoplasmic tail interacting with the cytoplasmic protein Tip20p. Because a strain lacking both C. albicans SEC20alleles could not be constructed, we placed SEC20 under transcriptional control of two regulatable promoters, MET3pand PCK1p. Repression of SEC20 expression in these strains prevented (MET3p-SEC20 allele) or retarded (PCK1p-SEC20 allele) growth and led to the appearance of extensive intracellular membranes, which frequently formed stacks. Reduced SEC20 expression in the PCK1p-SEC20strain did not affect morphogenesis but led to a series of hypersensitivity phenotypes including supersensitivity to aminoglycoside antibiotics, to nystatin, to sodium dodecyl sulfate, and to cell wall inhibitors. These results demonstrate the occurrence and function of Sec20p in a fungal species other than S. cerevisiae, but the lack of the N-terminal domain and the apparent absence of a close TIP20 homolog in the C. albicans genome also indicate a considerable diversity in mechanisms of retrograde vesicle traffic in eukaryotes.

scholarly journals Fungal Community Analysis and Biodeterioration of Waterlogged Wooden Lacquerware from the Nanhai No. 1 Shipwreck

2020 ◽  
Vol 10 (11) ◽  
pp. 3797
Author(s):  
Yin Jia ◽  
Liuyu Yin ◽  
Fengyu Zhang ◽  
Mei Wang ◽  
Mingliang Sun ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
High Throughput Sequencing ◽  
Environmental Parameters ◽  
Community Analysis ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Fungal Communities ◽  
Cellulolytic Enzymes ◽  
Biocidal Products

To avoid the lacquerware of the Nanhai No. 1 shipwreck from being corroded by microorganisms and to improve the knowledge on microbial ecology of the wood lacquers, we conducted a series of tests on the two water samples storing the lacquerware and colonies on the surface of the lacquerware. The high-throughput sequencing detected dominant fungal communities. After that, the fungal strains were isolated and then identified by amplification of ITS- 18S rRNA. Then the activity of ligninolytic and cellulolytic enzymes was detected on potato dextrose agar (PDA) plates with 0.04% (v/v) guaiacol and carboxymethyl cellulose (CMC) agar plates. Finally, we tested the biocide susceptibility of these fungi. Penicillium chrysogenum (NK-NH3) and Fusarium solani (NK- NH1) were the dominant fungi in the sample collected in April 2016 and June 2017. What is more, both showed activity of ligninolytic and cellulolytic enzymes. Four biocidal products (Preventol® D7, P91, BIT 20N, and Euxyl® K100) inhibited the growth of the fungal species in vitro effectively. In further research, the microbial community and environmental parameters in the museum should be monitored to assess the changes in the community and to detect potential microbial outbreaks.

On the suitability of induction heating system for metal additive manufacturing

2020 ◽  
Vol 235 (1-2) ◽  
pp. 219-229 ◽  
Author(s):  
Gourav K Sharma ◽  
Piyush Pant ◽  
Prashant K Jain ◽  
Pavan K Kankar ◽  
Puneet Tandon
Keyword(s):  
Energy Source ◽  
Additive Manufacturing ◽  
Induction Heating ◽  
Low Cost ◽  
Three Dimensional ◽  
Experimental System ◽  
Heating System ◽  
Industrial Applications ◽  
Metallic Materials ◽  
Semi Solid

Induction heating is a non-contact-based energy source that has the potential to quickly melt the metal and become the alternate energy source that can be used for additive manufacturing. At present, induction heating is widely used in various industrial applications such as melting, preheating, heat treatment, welding, and brazing. The potential of this source has not been explored in the additive manufacturing domain. However, the use of induction heating in additive manufacturing could lead to low-cost part fabrication as compared to other energy sources such as laser or electron beam. Therefore, this study explores the feasibility of this energy source in additive manufacturing for fabricating parts of metallic materials. An experimental system has been developed by modifying an existing delta three-dimensional printer. An induction heater coil has been incorporated to extruder head for semi-solid processing of the metal alloy. In order to test the viability of the developed system, aluminium material in the filament form has been processed. Obtained results have shown that the induction heating–based energy source is capable of processing metallic materials having a melting point up to 1000° C. The continuous extrusion of the material has been achieved by controlling the extruder temperature using a proportional integral derivative–based controller and k-type thermocouple. The study also discusses various issues and challenges that occurred during the melting of metal with induction heating. The outcomes of this study may be a breakthrough in the area of metal-based additive manufacturing.

scholarly journals PROTOTYPE DESIGN OF MICRO HYDRO USING TURBINE ARCHIMEDES SCREW FOR SIMULATION OF HIDROPOWER PRACTICAL OF ELECTRO ENGINEERING STUDENTS

2019 ◽  
Vol 3 (1) ◽  
pp. 6
Author(s):  
Antonius Ibi Weking ◽  
Yanu Prapto Sudarmojo
Keyword(s):  
Energy Source ◽  
Power Plants ◽  
Engineering Students ◽  
Low Cost ◽  
Water Pressure ◽  
Water Discharge ◽  
Small Scale ◽  
Engineering Department ◽  
Influence Of Water ◽  
Turbine Speed

Development of new and renewable energy source always developed by world researchers which one of those is energy source from water, because it’s friendly for environment and low cost. Water is one of energy source which it’s have big potential in all Indonesian territory. The main problem from microhydropower plants is a water discharge which it’s flow is not continued every year because influenced by weather season. Micro Hydro is a microhydro power plants (MHP)in a small scale. A micro hydro can be operated in a certain of time if it has a enough water supply. To knowing a right of micro hydro’s  characteristic is not easy thing to learn it. It is because a characteristic each of micro hydro’s installation location is considered specific location.               One type of micro hydro is using Archimedes Screw Turbine. Udayana University of Electrical Engineering Department in this time not have a facility for hydropower field to use this model, so a college student not yet to receive a knowledge of this. Through this research, a writer want to expand a college student’s knowledge in hydropower field with create a prototype of micro hydro with Archimedes Screw Turbine to hydropower practical in laboratory. In this research the effect of screw’s height angle conversion and effect of water pressure conversion has to be researched. In this study will discuss the influence of water pressure and slope of the altitude angle on the rotation produced by the Archimedes screw turbine so that it can be seen the voltage, current, power generated by the generator, torque and efficiency . The result of from handmade equipment for this research in angle 40­0 with biggest generator round (rpm) is 3768 (rpm) and highest power is 10.92057 watt, torque adalah 0.60 Nm dan efisiensi sebesar 14 %. The torque which resulted from water pressure 24 psi is 0,73 Nm and efficience 18,01 %. The voltage, current, and output power which resulted in generator is 85,8 Volt, 0,1963 Ampere and 16,85 Watt. For generator speed round in the pressure 24 Psi is 4582 rpm, while turbine speed round which resulted from the pressure 24 Psi is 383 rpm before coupled with generator and 222 rpm after coupled with generator.

Sign in / Sign up

Export Citation Format

Share Document