Lipid content of cured tobacco

1974 ◽  
Vol 52 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Momtaz K. Wassef ◽  
James W. Hendrix

Samples from cured burley and flue-cured tobacco were examined for total lipid content and for lipid composition of fractionated lipid classes. Cured burley tobacco contained 6–8% lipids compared with 14–15% in cured flue-cured tobacco. Neutral lipids constituted 70–80% of burley lipids but only half the flue-cured lipids; the latter had 75% more neutral lipids than the former. Flue-cured tobacco contained considerably more (5–10 times) medium- to high-polarity lipids than burley. Stalk position had only a minor effect on the lipid content of both tobacco types.

2007 ◽  
Vol 2 (2) ◽  
pp. 75
Author(s):  
Gede Suwarthama Sumiarsa ◽  
Ronald P. Phelps

<p>Lipid and fatty acid profiles were described for copepod nauplii Apocy clops panamensis from fertilized brackish water ponds, and after being acclimated to fullsea water salinity. Mean total lipid content of copepod nauplii collected from ponds fertilized with inorganic fertilizer combined either with alfalfa meal, rice bran, wheat bran, and a combination of these fertilizers ranged from 5.66 ± 0.15 to 7.76% ± 0.27%. Non-polar (neutral) lipid fraction of pond copepod nauplii was a significantly higher percentage of the total lipid content (74.5 ± 1.8 - 93.5% ± 1.0%) compared to those of polar lipid (6.5 ± 1.0 - 21.3% ± 1.8%) (P= 0.000). DHA/EPA ratio in neutral lipids ranged from 1.8 ± 0.2 - 2.0 ± 0.1 with no significant differences in three fertilization regimes. DHA was 27.5% ± 0.56% of the neutral lipids and EPA 14.8% ± 0.8%. Acclimation of copepod nauplii for six hours from brackish to full-sea water salinity reduced their lipid content and individual dry weight significantly. Mean total lipid content was reduced 44.2%, non-polar lipid was reduced 46.9% and polar lipid was reduced 24.4%. Acclimation altered the DHA/EPA ratio, in the neutral fraction the ratio increased 26.3% but in the polar fraction it decreased 25%.</p>


2019 ◽  
Vol 19 (1) ◽  
pp. 87-91
Author(s):  
Nguyen Ba Kien ◽  
Trinh Thi Thu Huong ◽  
Luu Van Huyen ◽  
Nguyen Thanh Vinh ◽  
Tran Duy Phong ◽  
...  

For the first time, the total lipid content and lipid class composition of  the Vietnamese soft corals (Sinularia brassica, Sinularia flexibilis) and fire corals (Millepora dichotoma, Millepora platyphylla) were investigated. The results indicated that the total lipid content of the investigated species was significantly different. Compositions of the lipid classes were analyzed using TLC and image analysis program Sorbfil TLC Videodensitometer DV and the results showed that phospholipids (PL, 10.91–16.02%), monoalkyldiacylglycerols (MADAG, 20.69-39.92%) and hydrocarbon wax (HW, 29.83-37.17%) were the main lipid classes of the total lipid in soft coral species. Meanwhile, PL (24.11-33.23%), TG (14.27–34.92%), ST (10.10–14.50%) and HW (12.08–19.95%) were predominant in fire coral species. ST, TG and FFA contents in soft and fire corals were at low level. DG was only present in the Sinularia flexibilis but not in other studied corals.


Author(s):  
Ravi Kant ◽  
S. K. Atreja ◽  
S. S. Hasan

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonate-buffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) and acrosome reacted spermatozoa (115.7±2.04 μg/108 cells) were found significantly (P<0.05) different. During the process of capacitation and acrosome reaction (AR) there was substantial loss of total lipids. The total Lipid content of freshly ejaculated, capacitated and acrosome reacted spermatozoa were fractionated into three major classes namely, Phospholipids, Neutral lipids and Glycolipids. Among the classes of lipids; there was no change in concentration of glycolipids. Phospholipids in fresh, capacitated and acrosome reacted spermatozoa were 84.27±1.96, 58.40±2.40 and 50.51±1.27 μg/108 cells respectively. In case of Neutral lipids, the concentration were 33.63±2.62, 22.55±1.86 and 11.61±0.87 μg/108 cells in fresh, capacitated and acrosome reacted spermatozoa respectively. The results generated by this study will be one step further to understand the lipid changes in buffalo spermatozoa during capacitation, acrosome reaction and fertilization process.


2017 ◽  
Vol 31 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Greg Ford ◽  
Kandan Sethumadhavan ◽  
Carol Carter-Wientjes ◽  
John Bland ◽  
...  

2013 ◽  
Vol 37 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Geun Ho Gim ◽  
Jung Kon Kim ◽  
Hyeon Seok Kim ◽  
Mathur Nadarajan Kathiravan ◽  
Hetong Yang ◽  
...  

Author(s):  
Tereza Pavlova ◽  
Zdenek Spacil ◽  
Veronika Vidova ◽  
Filip Zlamal ◽  
Eliska Cechova ◽  
...  

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods &amp; Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1&ndash;3, 12&ndash;14, 29&ndash;30, 88&ndash;90 and 178&ndash;180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.


1993 ◽  
Vol 41 (3) ◽  
pp. 205-219
Author(s):  
C.J. Van Nevel ◽  
S. De Smet ◽  
D.I. Demeyer

Defaunated then refaunated sheep were given diets containing soyabean oil hydrolysate (SOH: 70 g/day) or an equivalent amount of lipids administered as crushed toasted soyabeans (TSB). Defaunation increased molar percentage of propionate in the rumen, while butyrate decreased. SOH caused a similar effect in both the defaunated and refaunated rumen, while the effect on acetate proportions was variable. Protozoal counts were lower after feeding SOH. Crushed toasted soyabeans had a minor effect on rumen fermentation pattern. Rumen digestibility of organic matter was decreased by both defaunation and SOH feeding, with a concomitant shift in digestion to the lower intestinal tract. Total tract digestibility was not affected. Both treatments increased nonammonia N flows at the duodenum, but this was only significant with defaunation. Total tract digestion of N remained almost constant. Defaunation resulted in more microbial protein reaching the duodenum. Except for the TSB diet, total lipid leaving the rumen equalled intake. Total tract digestibility of total lipid was much higher with SOH and TSB than with controls. Defaunation almost doubled microbial growth efficiency and this value tended to increase by SOH feeding. The decrease of protozoal count or even elimination of protozoa after lipid feeding could not entirely explain the change in rumen metabolism, as additional changes in defaunated sheep were shown.


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