Evaluating the potential of using 5-azacytidine as an epimutagen

A number of early flowering lines were induced when 5-azacytidine was applied to germinating flax (Linum usitatissimum L.) seed. The genetics of these lines indicate that the induced changes are epigenetic and probably result from demethylation of the genomic DNA at loci that affect flowering age. Although the growth and development of three stable early flowering lines are altered and the percentage of filled seed was reduced in all three lines compared with controls, measures of seed productivity demonstrated that harvest index was unaffected in two of the lines. In the third, harvest index was lower than normal and both seed set per capsule and seed mass per 100 seed were reduced. Furthermore, six generations after induction this line began to display relatively high levels of polyembryony. The late appearance of this twinning and other aspects related to working with lines induced by 5-azacytidine and using 5-azacytidine as an epimutagen are discussed.

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Studies of haploid–diploid twins in flax (Linum usitatissimum)

Canadian Journal of Genetics and Cytology ◽

10.1139/g85-055 ◽

1985 ◽

Vol 27 (4) ◽

pp. 371-379 ◽

Cited By ~ 4

Author(s):

Beatrice E. Murray

Keyword(s):

Survival Rate ◽

Linum Usitatissimum ◽

Seed Set ◽

Embryo Sac ◽

Flower Color ◽

Blue Flower ◽

Linum Usitatissimum L ◽

Chromosomal Pairing ◽

Haploid Embryos ◽

Double Cross

The present study was designed to investigate the origin of twin seedlings in flax (Linum usitatissimum L.). The twins were derived from crosses of high-twinning × nontwinning lines. The data presented were based on the following: (i) the morphology of twins in terms of variations in the position, orientation, and size of the twin embryos in the embryo sac and in the mature seed; (ii) the cytological interpretation of meiosis in the haploid and diploid member of F2 twins, and chromosomal pairing of the F3 diploid, triploid, and trisomic progeny obtained from crossing haploid × diploid F2 twins; and (iii) the genetic analysis of twinning frequencies, seed set by twins in single and double cross progenies, and the combinations of flower color phenotypes in F2 twins derived from blue flower × white flower crosses. The results, based on the survival rate of the haploid embryos, the cytological interpretation of meiosis, the fertility in the haploids, and in particular, the flower phenotype of twins in nine F2 families, support the hypothesis that the progenitors of the twin embryos are two-megaspore nuclei rather than one.Key words: flax twins.

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Studies on Correlation and Path Analysis in Linseed (Linum usitatissimum L.) Over Locations in Mid-hills of North-Western Himalayas

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5863 ◽

2021 ◽

Author(s):

Garima Thakur ◽

Satish Paul ◽

Uttam Chandel ◽

Ronika Thakur

Keyword(s):

Grain Yield ◽

Seed Yield ◽

Linum Usitatissimum ◽

Seed Weight ◽

Harvest Index ◽

Significant Positive Correlation ◽

Agricultural Research ◽

Positive Correlation ◽

Linum Usitatissimum L ◽

Important Trait

Background: Seed yield is a very important trait for selection but shows low heritability and hence is difficult to improve. Since the productivity of linseed (Linum usitatissimum L.) in India as well as Himachal Pradesh is low in comparison to other major linseed growing countries and states, improvement in cultivars for grain yield is a must. Therefore, the present study was aimed for studying the character associations in linseed genotypes for seed yield over locations. Method: The experiment was conducted during rabi 2019-2020. The experimental material for the present investigation comprised of 52 linseed genotypes grown over three locations in Himachal Pradesh i.e. Linseed Experimental Farm, CSK HPKV, Palampur (1290 m amsl), Shivalik Agricultural Research and Extension Centre, Kangra (700 m amsl) and Hill Agricultural Research and Extension Centre, Dhaulakuan (468 m amsl). Randomized block design with three replications was used. Phenotypic and genotypic correlation coefficients were worked out as per the procedure outlined by Burton and De Vane (1953) and Johnson et al. (1955). Direct and indirect effects of component traits on grain yield were worked out using correlation coefficient of various traits as suggested by Wright (1921) and elaborated by Dewey and Lu (1959). Result: Correlation studies indicated highly significant positive correlation for seed yield with 1000 seed weight (0.965**) followed by harvest index (0.801**), secondary branches (0.585**) and a significant correlation with biological yield (0.269**). Seed yield exhibited a non-significant positive correlation with seeds per capsule. However, a negative significant correlation was observed for seed yield with days to 50 per cent flowering and number of primary branches. The path coefficient analysis indicated that 1000 seed weight exhibited maximum positive direct effect with seed yield (0.741) while others had a low direct effect. The significant positive correlation of number of secondary branches and harvest index with seed yield was mainly due to indirect effect via 1000 seed weight indicating that 1000 seed weight is the most important trait for the improvement of grain yield in linseed as per the present study.

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CREATION OF LINUM USITATISSIMUM INITIAL MATERIAL WITH MARKER SIGN – YELLOW-COLORED SEEDS

TAURIDA HERALD OF THE AGRARIAN SCIENCES ◽

10.33952/2542-0720-2021-3-27-135-143 ◽

2021 ◽

Vol 3 (27) ◽

pp. 135-143

Author(s):

V.P. Ponazhev ◽

Keyword(s):

Seed Yield ◽

Linum Usitatissimum ◽

Seed Weight ◽

Seed Mass ◽

Point Of View ◽

Breeding Value ◽

Initial Material ◽

Standard Variety ◽

Fiber Flax ◽

Linum Usitatissimum L

Creation of the initial material of fiber flax (Linum usitatissimum L.) with marker signs is a promising direction of breeding, which determines the efficiency of breeding varieties with improved properties. The purpose of the research was to create initial flax material for obtaining seeds with a marker sign (yellow color) and increased weight. The studies were carried out at the Federal Research Center for Bast Fiber Crops in 2015–2018. In our work, we used flax varieties ‘Smolich’, ‘Antey’, ‘C-108’, as well as forms obtained from these varieties. These varieties were used as control. Nurseries were laid under conditions of a leveled agricultural background. Seeds were sown in a square way (2.5×2.5 cm). The experiments were conducted according to the current methods, including phytopathological testing. Soil – sod-podzolic, medium loamy, cultivated. Selyaninov Hydrothermal Coefficient (HTC) in 2015 was 1.3; in 2016 – 2.2; in 2017 – 1.8; in 2018 – 1.3. During the flax plants selection, we obtained three forms with yellow-colored seeds. Yellow-seeded form No. 1 was valuable from the breeding point of view. It exceeded standard variety ‘Smolich’ in seed weight by 0.8 mg (16.0%), in seed yield – by 0.06 t/ha (9.8%). Form No. 1 belongs to the improved fiber flax line. Form No. 3 had a higher seed mass (5.6 mg) compared to standard variety ‘C-108’. In terms of seed yield and 1000-seed weight, this form exceeded the standard by 0.08 t/ha or 14.8% and 0.49 g or 10.6%, respectively. Form No. 3 is a breeding material for creating varieties of bilateral use. Form No. 2 had signs of the oil flax line. It exceeded standard variety ‘Antey’ in seed yield by 0.18 t/ha (40%); 1000-seed weight – by 0.53 g (11.2%). The created material has a breeding value. It was transferred to the bioresource collection of flax for its replenishment, to the bank of the characteristic collection and was additionally included in the breeding process.

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Ecological and genetic aspects of productivity and quality in linseed (Linum usitatissimum L.) cultivars

Ecological genetics ◽

10.17816/ecogen7414-22 ◽

2009 ◽

Vol 7 (4) ◽

pp. 14-22

Author(s):

Svetlana I Vakula ◽

Lidia V Koren ◽

Olga S Ignatovets ◽

Vladimir V Titok ◽

Lubov V Khotyleva

Keyword(s):

Environmental Conditions ◽

Linum Usitatissimum ◽

Geographical Origin ◽

Ecological Factors ◽

Qualitative Composition ◽

Seed Productivity ◽

Oil Accumulation ◽

Linum Usitatissimum L ◽

Growing Conditions

The article describes differences in a response of linseed cultivars of a different genetic and geographical origin to a set of ecological factors. Variability in productivity traits was revealed to be determined in most cases by a genotype and its interaction with growing conditions. The genotype and environmental conditions exert an equal effect on oil accumulation in seeds and its qualitative composition. Based on the data obtained from the linseed collection, the most ecologically stable genotypes, displaying steadily high seed productivity in combination with high content and quality of oil, were selected.

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Genetic variability, mean performance and analysis of variance in Linseed (Linum usitatissimum L.)

International Journal of Agricultural Invention ◽

10.46492/ijai/2019.4.1.15 ◽

2019 ◽

Vol 4 (01) ◽

pp. 89-95

Author(s):

Ravindra Kumar Kasana ◽

P. K. Singh ◽

Amit Tomar ◽

Mahak Singh

Keyword(s):

Genetic Variability ◽

Plant Height ◽

Analysis Of Variance ◽

Seed Yield ◽

Linum Usitatissimum ◽

Harvest Index ◽

Linum Usitatissimum L ◽

Days To Maturity ◽

Biological Yield ◽

Number Of Seeds

The most promising lines showing highest seed yield with high mean performance for other yield contributing characters were identified as EC-22672, NDLS-164, NDLS-169 and NDLS-205-03-02. The genotypes exhibiting highest mean performance for different characters such as EC-22672 for seed yield per plant, SLS-50, NDL-204, Meera and NDLS-204 for early days to 50% flowering, NDLS-181 for early days to maturity, EC- 41494 for plant height, NDLS-77 for primary branches per plant, NDLS-169 for secondary branches per plant, EC-41404 for number of capsules per plant, EC-41494 for number of seeds per capsule, Shekhar for biological yield, EC-23269 for harvest index and SLS-48 for test weight.

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