Changes in antioxidant enzymes in postmortem muscle and effects on meat quality from three duck breeds during cold storage

2020 ◽  
Vol 100 (2) ◽  
pp. 234-243
Author(s):  
Nenzhu Zheng ◽  
Linli Zhang ◽  
Qingwu Xin ◽  
Zhongwei Miao ◽  
Zhiming Zhu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Meat Quality ◽  
Cold Storage ◽  
Antioxidant Enzyme Activity ◽  
Muscovy Duck ◽  
Meat Color ◽  
Muscle Tissues

This study was conducted to evaluate changes to antioxidant systems in the postmortem muscle of three duck breeds and to analyze their relationship with meat quality. Pekin ducks, Muscovy ducks, and Mulard ducks were euthanized at the age of 70 d. The antioxidant enzyme activities and related gene expressions as well as meat quality in muscle tissues were examined. The breed of the duck had a significant effect on the antioxidant capacity of muscle tissues (P < 0.05), with the exceptions of superoxide dismutase (SOD) activity at 96 h as well as total antioxidant capacity (T-AOC) at 120 h. The SOD, glutathione peroxidase (GPx/GSH-Px), and T-AOC activities from highest to lowest were Muscovy duck > Mulard duck > Pekin duck, whereas the malondialdehyde (MDA) concentration followed the opposite pattern. During cold storage (0–120 h), a decrease in the GPx, SOD, and T-AOC activities was noted, although MDA concentration increased gradually. The GPx1 and Cu/Zn-SOD gene expression levels in Muscovy duck muscle tissues were significantly higher than those in other breeds at both 0 and 24 h. Correlation analysis showed that higher antioxidant enzyme activity in duck muscle tissues was associated with higher water-holding capacity (WHC) and more stable meat color. Higher antioxidant enzyme activity in duck meat similarly resulted in higher pH values, higher WHC, and more stable meat color. These data indicate that antioxidant enzymes may inhibit lipid oxidation and participate in the regulation of meat quality.

scholarly journals Effect of Pulsed Ultraviolet Light on Natural Microbial Load and Antioxidant Properties of Fresh Blueberries

2018 ◽  
Vol 6 (6) ◽  
pp. 733
Author(s):  
Senem Guner ◽  
Zeynal Topalcengiz
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Antioxidant Enzyme Activity ◽  
Microbial Load ◽  
Plate Count ◽  
Light Illumination ◽  
Sod Activity ◽  
Aerobic Plate Count

Blueberries have been drawn more attention in recent years for their antioxidant capacities and proposed health benefits. Pulsed ultraviolet (PUV) light is known for its disinfection effects on the surface of fresh fruits. This study’s aim was to examine the effects of PUV light on naturally present microbial load, antioxidant capacity, and antioxidant enzyme activity of fresh blueberries. Fresh blueberry samples were treated for 30, 60, 90, and 120 s. Samples were stabilized at 13 cm away from the PUV lamp before treatment. Total Aerobic Plate Count (TAPC) and yeast and mold count reduction were observed the highest in samples treated for 90 s, 1.97 and 1.27 log CFU/g, respectively. Antioxidant capacity levels were significantly different for treated samples for 60 and 90 s in FRAP analysis, whereas no differences were observed in results of DPPH analysis. Superoxide dismutase (SOD) activity as antioxidant enzyme had no difference, however, glutathione peroxidase (GPx) increased by 50% with PUV treatment for 120 s. PUV light treatment of fresh blueberries for 90 s was determined as ideal exposure time among tested time intervals of this study. PUV light illumination can generally enhance antioxidant capacity and antioxidant enzyme activity of fresh blueberries and decrease the population of natural microorganisms of fresh blueberries and similar small fruits.

Evaluation of antioxidant enzyme activity and antioxidant capacity in patients with newly diagnosed pemphigus vulgaris

2015 ◽  
Vol 40 (3) ◽  
pp. 313-317 ◽  
Author(s):  
M. H. Javanbakht ◽  
M. Djalali ◽  
M. Daneshpazhooh ◽  
M. Zarei ◽  
M. R. Eshraghian ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Pemphigus Vulgaris ◽  
Antioxidant Enzyme Activity ◽  
Newly Diagnosed

scholarly journals Comparison of fatty acids profile and antioxidant enzyme level of cladoceran Moina brachiata (jurine, 1820) from freshwater bodies of Chennai

2017 ◽  
Vol 5 (1) ◽  
pp. 87
Author(s):  
Gomathi Jeyam. M ◽  
Ramanibai Ravichandran
Keyword(s):  
Fatty Acids ◽  
Dna Damage ◽  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Enzyme Level ◽  
Antioxidant Enzyme Activity ◽  
Omega 3 ◽  
Pufa Content ◽  
Algal Diet

Omega-3 family (ω-3) of polyunsaturated fatty acids (PUFA) was considered as an important biochemical for the physiological function of all trophic level animals. In this study, we demonstrated the effect of algal diet on fatty acids composition (FA), antioxidant enzymes and DNA damage of Moina brachiata from Adyar River and Kolavoi Lake. 8 different fatty acids were identified in M. brachiata through GC-MS analysis and we noticed two PUFA (Eicosapentaenoic acid, EPA 20:5 (ω-3); Linoleic acid 18:2 (ω-6)). The dietary fatty acid accumulation and bioconversion capacity of M. brachiata have differed in two lakes fed with algal diet. The high amount of ω-3 PUFA was observed in M. brachiata fed with Scenedesmus sp. in Kolavoi Lake (35.84%) followed by Adyar River (33.78%). PUFA content was significantly declined in wild M. brachiata of Adyar River (17.44%) followed by Kolavoi lake (25.78%). On the other hand, high level of Malondialdehyde (MDA) and decreasing level of key antioxidant enzymes likes Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GSH) and DNA damage were observed in wild M. brachiata of Adyar River. Hence, the algal diet could enhance the level of antioxidant enzyme activity by decreasing the level of MDA and it does not show DNA damage on M. brachiata. Overall, the results obtained in this study explored that Scenedesmus sp., has the ability to enhance the PUFA content, antioxidant enzyme activity and prevent the DNA damage in M. brachiata which was declined in the wild animal due to the environmental stress conditions.

scholarly journals Chilling injury in pineapple fruits: physical quality attributes and antioxidant enzyme activity

Food Research ◽  
2020 ◽  
Vol 4 (S5) ◽  
pp. 86-95
Author(s):  
N.H. Dolhaji ◽  
I.D. Muhammad ◽  
H. Yaakob ◽  
A. Mohd Marsin
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Cold Storage ◽  
Nutritional Value ◽  
Chilling Injury ◽  
Quality Attributes ◽  
Antioxidant Enzyme Activity ◽  
Cold Chain ◽  
Physical Quality ◽  
Related Enzyme

Harvested fruit have high metabolic and moisture content which leads to an active biochemical reaction that contributes to decrement of nutritional value such as vitamin, proteins and lipids. The application of low temperature as a single-effective management to prolong shelf-life of fruits is a common practice which applied to keep agriculture commodities at high quality. A matured stage pineapple is very perishable and cold storage chain is crucial in maintaining the chemical and physical quality attributes in order to assure its commercial value for market. The main objective of this study is to evaluate the effect of sub-optimum cold storage scenario on changes of pineapple fruits physical quality attributes, the activity of browning enzyme and antioxidant related enzyme in 2 pineapple cultivars (cv.), Morris (Queen-type) and Josapine (hybrid of Spanish and Smooth Cayeen). Malaysian pineapple fruit cv. with different sensitivity toward CI, Morris (Queen-type) and Josapine (Smooth-Cayenne-type) were stored at sub-optimal storage temperatures (4±2°C) for 28 days to investigate the effects of CI towards physical quality attributes and antioxidant enzyme activity. The result indicated both cv. was affected with CI towards the 28 days of sub-optimum cold storage. Overall physical quality attributes indicated CI was found positively correlated with the increase of EL and TTA and on the contrary decrease its firmness, weight (% w/w), brightness (L*) and redness (A*). Similarly, a positive correlation was also deduced between CI symptoms and the activities of PPO and APX which reflect the incident of oxidative stress. The results derived from this study may serve as a basis for evaluation of better postharvest strategies to control CI during cold chain storage of pineapple fruits and thus assure the quality and nutritional value till it reaches to consumer.

scholarly journals Antioxidant enzyme activity in the muscles of calves depleted of vitamin E or selenium or both

1993 ◽  
Vol 70 (2) ◽  
pp. 621-630 ◽  
Author(s):  
Dominic M. Walsh ◽  
D. Glenn Kennedy ◽  
Edward A. Goodall ◽  
Seamus Kennedy
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Vitamin E ◽  
Antioxidant Enzyme ◽  
Glutathione Transferase ◽  
Basal Diet ◽  
Antioxidant Enzyme Activity ◽  
Set Up ◽  
Feeding Diets ◽  
Glucose 6 Phosphate Dehydrogenase

Feeding diets depleted of vitamin E and Se to cattle can induce a disease known as nutritional degenerative myopathy. It is believed that an increased peroxidative challenge in muscle is involved in the pathogenesis of this disease. A number of species can up-regulate the activity of some antioxidant enzymes, including glutathione reductase (EC 1.6.4.2), glutathione transferase (EC 2.5.1.18), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), catalase (EC 1.11.1.6), and superoxide dismutase (EC 1.15.1.1), in an attempt to mitigate the effects of a peroxidative challenge. A 2 × 2 factorial study was set up to examine possible changes in the activities of these antioxidant enzymes in muscles of ruminant calves fed on diets low in either vitamin E or Se. Four groups of four calves each were fed on a basal diet of NaOH-treated barley which was supplemented with α-tocopherol or Se or both for a total of 50 weeks. Calves fed on diets depleted of vitamin E, but not those ted on diets low in Se, developed subclinical myopathy, as judged by increases in the activity of plasma creatine kinase (EC 2.7.3.2), and had increased muscle concentrations of two indices of lipid peroxidation, namely thiobarbituric acidreactive substances, with and without ascorbate activation. Feeding diets depleted of vitamin E and diets low in Se both increased muscle activities of glucose-6-phosphate dehydregenase in heart, biceps and supraspinatus. This change may have occurred in an attempt to maintain intracellular pools of reduced glutathione. No other changes in antioxidant enzyme activity were observed.

Carnitine-mediated antioxidant enzyme activity and Bcl2 expression involves peroxisome proliferator-activated receptor-γ coactivator-1α in mouse testis

2017 ◽  
Vol 29 (6) ◽  
pp. 1057 ◽  
Author(s):  
Vikas Kumar Roy ◽  
Rachna Verma ◽  
Amitabh Krishna
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Serum Testosterone ◽  
Antioxidant Enzyme Activity ◽  
Mouse Testis ◽  
Peroxisome Proliferator ◽  
Dependent Manner ◽  
Peroxisome Proliferator Activated Receptor ◽  
Bcl2 Expression

The protective effects of carnitine have been attributed to inhibition of apoptosis, alleviating oxidative stress and DNA repair mechanism by decreasing oxidative radicles. Carnitine also increases mitochondrial biogenesis via peroxisome proliferator-activated receptor-γ coactivator-1α (PGC1α). The role of carnitine in testicular PGC1α expression has not been documented. We hypothesised that the effects of carnitine as an antioxidant, inhibitor of apoptosis and controller of steroidogenesis in mouse testis may involve PGC1α as a regulator. The present study was designed to evaluate the localisation of PGC1α and the effects of carnitine treatment on the expression of PGC1α, Bcl2 and antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) in mouse testis and serum testosterone concentrations. PGC1α was primarily immunolocalised to the Leydig cells and primary spermatocytes. Western blot analysis showed that carnitine (50 mg kg–1 and 100 mg kg–1 for 7 days) significantly increased PGC1α and Bcl2 expression in the testis in a dose-dependent manner. In addition, carnitine treatment significantly increased antioxidant enzyme (CAT, SOD and GPx) levels. The carnitine-induced changes in PGC1α in the testis were significantly correlated with changes in serum testosterone concentrations, as well as with changes in Bcl2 expression and antioxidant enzyme activity in the testis, as evaluated by electrophoresis. Therefore, the results of the present study suggest that carnitine treatment of mice increases PGC1α levels in the testis, which may, in turn, regulate steroidogenesis by increasing expression of Bcl2 and antioxidant enzymes.

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