Optimization of liquid media and biosafety assessment for algae-lysing bacterium NP23

2014 ◽  
Vol 60 (9) ◽  
pp. 593-597 ◽  
Author(s):  
Chunli Liao ◽  
Xiaobo Liu ◽  
Linna Shan
Keyword(s):  
Culture Medium ◽  
Cell Concentration ◽  
Chlorella Pyrenoidosa ◽  
Algal Culture ◽  
High Cell Concentration ◽  
Test Species ◽  
Lysis Rate ◽  
Burman Design ◽  
Microcystis Wesenbergii ◽  
Mutagenic Effects

To control algal bloom caused by nutrient pollution, a wild-type algae-lysing bacterium was isolated from the Baiguishan reservoir in Henan province of China and identified as Enterobacter sp. strain NP23. Algal culture medium was optimized by applying a Placket–Burman design to obtain a high cell concentration of NP23. Three minerals (i.e., 0.6% KNO3, 0.001% MnSO4·H2O, and 0.3% K2HPO4) were found to be independent factors critical for obtaining the highest cell concentration of 1013 CFU/mL, which was 104 times that of the control. In the algae-lysing experiment, the strain exhibited a high lysis rate for the 4 algae test species, namely, Chlorella vulgari, Scenedesmus, Microcystis wesenbergii, and Chlorella pyrenoidosa. Acute toxicity and mutagenicity tests showed that the bacterium NP23 had no toxic and mutagenic effects on fish, even in large doses such as 107 or 109 CFU/mL. Thus, Enterobacter sp. strain NP23 has strong potential application in the microbial algae-lysing project.

scholarly journals Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 627-632 ◽  
Author(s):  
O.G. Nagel ◽  
M.P. Molina ◽  
R.L. Althaus
Keyword(s):  
Experimental Design ◽  
Logistic Model ◽  
Logistic Regression Model ◽  
Culture Medium ◽  
Spore Concentration ◽  
Microbiological Method ◽  
Burman Design ◽  
Detection Capabilities ◽  
Sulfonamide Residues ◽  
Plackett Burman Design

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2<sup>4-1</sup>). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

scholarly journals Nanobubbles activate anaerobic growth and metabolism of Pseudomonas aeruginosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miu Ito ◽  
Yuichi Sugai
Keyword(s):  
Carbon Dioxide ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Anaerobic Condition ◽  
Culture Medium ◽  
Cell Concentration ◽  
Essential Elements ◽  
Anaerobic Growth ◽  
Ferrous Ions ◽  
Positively Charged

AbstractThe effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. P. aeruginosa grew earlier in the culture medium containing nanobubbles and the bacterial cell concentration in that culture medium was increased a few times higher compared to the medium without nanobubbles under anaerobic condition. Both gas and protein, which are the metabolites of P. aeruginosa, were remarkably produced in the culture medium containing nanobubbles whereas those metabolites were little detected in the medium without nanobubbles, indicating nanobubbles activated anaerobic growth and metabolism of P. aeruginosa. The carbon dioxide nanobubbles came to be positively charged by adsorbing cations and delivered ferrous ions, one of the trace essential elements for bacterial growth, to the microbial cells, which activated the growth and metabolism of P. aeruginosa. The oxygen nanobubbles activated the activities of P. aeruginosa as an oxygen source.

scholarly journals Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus

2005 ◽  
Vol 48 (spe) ◽  
pp. 97-104 ◽  
Author(s):  
Álvaro Baptista-Neto ◽  
Juliana Conceição Teodoro ◽  
Luiz Claudio Macedo Cassiano Filho ◽  
Alberto Colli Badino ◽  
Carlos Osamu Hokka
Keyword(s):  
Continuous Culture ◽  
Hollow Fiber ◽  
Cell Concentration ◽  
Continuous Cultivation ◽  
Batch Process ◽  
Streptomyces Clavuligerus ◽  
High Cell ◽  
Continuous Cultures ◽  
High Cell Concentration ◽  
Cell Recycling

The aim of the present work was to compare CA production in continuous culture with and without cell recycling and in batch process by Streptomyces clavuligerus. Continuous cultivations with high cell concentration using cell recycling were performed utilizing a hollow fiber ultrafiltration module to separate cells from the filtrate broth. The continuous cultures without cell recycling and the batch cultivations were performed conventionally. The highest productivity was attained in the continuous cultivation with cell recycling (22.2 mg.L-1.h-1). The highest CA concentration was obtained in the batch process (470 mg.L-1.h-1).

scholarly journals Preparation of an experimental low-fluoride diet from single-cell organisms for rats and mice

1980 ◽  
Vol 44 (3) ◽  
pp. 371-380 ◽  
Author(s):  
S. A. Khalawan ◽  
J. C. Elliott ◽  
R. W. Fearnhead
Keyword(s):  
Single Cell ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Chlorella Pyrenoidosa ◽  
Distilled Water ◽  
Yeast Biomass ◽  
Two Generations ◽  
Rats And Mice ◽  
Maize Oil

1. A method for producing a standard low-fluoride diet from a green alga and yeast is described. Chlorella pyrenoidosa was grown in a culture medium prepared with distilled water and analytical grade chemical salts. The spent culture medium from the alga culture was reclaimed and replenished with salts and sucrose for the production of yeast, Saccharomyces cerevisiae.2. The single-cell organisms were separated by centrifugation from their culture media and the dried cells were blended with sucrose, maize oil, cellulose and a salt mix to produce diet pellets for rats and mice.3. The diet was readily accepted as food by rats and mice and it was found to contain 100–300 μg fluoride/kg dry weight. Two generations of rats and four generations of mice were bred on this diet.4. The use of hydroxyapatite to reduce the fluoride content of the chemicals used in the production of the alga and yeast biomass was investigated. Diet pellets prepared with this biomass contained 45–60 μg fluoride/kg dry weight.

Satisfactory recovery and viability of stem cells cryopreserved at high cell concentration

1997 ◽  
Vol 18 (3) ◽  
pp. 399-403 ◽  
Author(s):  
P Perseghin ◽  
R Epis ◽  
M Vigano ◽  
A Malacrida ◽  
A Pastorini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Concentration ◽  
High Cell ◽  
High Cell Concentration ◽  
Satisfactory Recovery

scholarly journals Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

2005 ◽  
Vol 48 (spe) ◽  
pp. 85-95 ◽  
Author(s):  
Tássia Raffoul ◽  
Kamilla Swiech ◽  
Mabel Karina Arantes ◽  
Álvaro Paiva Braga de Sousa ◽  
Ronaldo Zucatelli Mendonça ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Culture Medium ◽  
Cell Concentration ◽  
Animal Cell ◽  
Positive Influence ◽  
Medium Supplement ◽  
Co2 Environment

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.

scholarly journals Seeking for optimum culture medium for chlorella pyrenoidosa and analysis of the amino acid component

2019 ◽  
Vol 1423 ◽  
pp. 012006
Author(s):  
Wei Li ◽  
Xiaojiang Chen ◽  
Mengmeng Zheng ◽  
Zhicheng Li ◽  
Haiyang Li
Keyword(s):  
Amino Acid ◽  
Culture Medium ◽  
Chlorella Pyrenoidosa ◽  
Acid Component ◽  
Amino Acid Component

Physiological and Ecological Significance of the Chemical Instability of Uric Acid and Related Purines in Sea Water and Marine Algal Culture Medium

1974 ◽  
Vol 31 (8) ◽  
pp. 1327-1335 ◽  
Author(s):  
Naval J. Antia ◽  
Arthur F. Landymore
Keyword(s):  
Uric Acid ◽  
Culture Medium ◽  
Sea Water ◽  
Major Effect ◽  
Small Degree ◽  
Algal Culture ◽  
Minor Effect ◽  
Chemical Instability ◽  
Concentration Changes ◽  
Marine Algal

The chemical instability of uric acid and related purine bases in a seawater phytoplankton culture medium was evaluated spectrophotometrically from concentration changes incurred on aseptic incubation at about 20 C under cool-white light or complete darkness. Uric acid showed slow degradation in darkness, which was increased severalfold by illumination. Xanthine showed a small degree of degradation only from illumination, but adenine, guanine, and hypoxanthine appeared to be stable under both test conditions. The degradation of uric acid was traced to three factors differing in intensity of effect: 1) pH of sea water (minor effect), 2) photolysis (additional minor effect), 3) trace-metal cation content of sea water (major effect), whereas that of xanthine could be traced only to photolysis.These results cast doubt on previous reports of utilization of uric acid as N-source for phototrophic growth of marine planktonic algae in that the compound actually utilized may be degradation product(s) rather than the intact purine. Ecologically, the results signify that uric acid could undergo rapid chemical turnover, without biological intervention, in the marine environment.

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