Pseudomonas species isolated from tobacco seed promote root growth and reduce lead contents in Nicotiana tobacum K326

2019 ◽  
Vol 65 (3) ◽  
pp. 214-223 ◽  
Author(s):  
Juan Li ◽  
Bufan Zheng ◽  
Ruiwen Hu ◽  
Yongjun Liu ◽  
Yongfeng Jing ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Root Growth ◽  
Endophytic Bacteria ◽  
Tobacco Seed ◽  
Nicotiana Tobacum ◽  
Tobacco Seeds ◽  
Pseudomonas Species ◽  
Phosphorus And Potassium ◽  
Indole 3 Acetic Acid ◽  
Tobacco Growth

Endophytic bacteria are generally helpful for plant growth and protection. We isolated from tobacco seeds three Pseudomonas strains (K03, Y04, and N05) that could produce siderophores, indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase, fix nitrogen, dissolve phosphorus and potassium, and tolerate heavy metals. In pot experiments, the three isolated strains significantly promoted root growth and increased the root enzyme activity in Nicotiana tobacum K326. Furthermore, bacterial inoculations increased the proportion of residual lead (Pb) by 8.36%–51.63% and decreased the total Pb content by 3.28%–6.38% in the contaminated soil during tobacco planting, compared with uninoculated soils. An effective decrease in Pb content was also found in tobacco leaves with bacterial inoculations. K03 inoculation decreased the Pb content in the upper leaves by 49.80%, and Y04 inoculation had the best effect, decreasing the Pb content in the middle leaves by 70.12%. Additionally, soil pH and root activity had significant effects on transformation and translocation of Pb. The study suggested that in response to Pb pollution in soil, a reasonable application of endophytes (e.g., Pseudomonas) might be a promising approach in promoting tobacco growth and reducing Pb content in tobacco, while simultaneously enhancing Pb stabilization in soils.

Biolixiviation des métaux lourds et stabilisation des boues d'épuration : essai en bioréacteur opéré en mode cuvée

1993 ◽  
Vol 20 (1) ◽  
pp. 57-64 ◽  
Author(s):  
R. D. Tyagi ◽  
J. F. Blais ◽  
N. Meunier ◽  
D. Kluepfel
Keyword(s):  
Heavy Metals ◽  
Sewage Sludge ◽  
Suspended Solids ◽  
Elemental Sulfur ◽  
Batch Reactor ◽  
Fecal Coliforms ◽  
Sludge Stabilization ◽  
Sludge Digestion ◽  
Batch Time ◽  
Phosphorus And Potassium

A biological process of heavy metals solubilization and sewage sludge stabilization was studied in a batch reactor of 30-L capacity. The acclimatized leaching microflora was composed of two major groups of thiobacilli: less acidophilic and acidophilic. A batch time of 10 days allows a substantial metal solubilization: cadmium (100%), copper (80%), manganese (80%), nickel (46%), and zinc (100%). The bioleaching process also causes a significative decrease in sludge total suspended solids (25%) and volatile suspended solids (32%), and a considerable reduction (under the detection limit of 10 cfu∙mL−1) of indicator bacteria (total coliforms, fecal coliforms, fecal streptococci). After filtration or centrifugation of the leached sludge, the solubilized metals were precipitated by lime neutralization. The phosphorus and potassium sludge contents were not affected by bioleaching process. These results indicate that the process of sludge digestion and metal leaching can be conducted in parallel in the same reactor. Key words: sewage sludge, heavy metals, bioleaching, stabilization, thiobacilli, elemental sulfur.

scholarly journals Restoration of soil fertility of the southern chernozem in conditions of the reserve regime of the Orenburg State Nature Reserve

2018 ◽  
Vol 7 (2) ◽  
pp. 126-129
Author(s):  
Alexander Nikolaevich Tyurin
Keyword(s):  
Heavy Metals ◽  
Soil Profile ◽  
Nature Reserve ◽  
Specially Protected Natural Areas ◽  
Southern Chernozem ◽  
State Nature Reserve ◽  
Protected Natural Areas ◽  
Phosphorus And Potassium ◽  
State Nature

This paper presents a comparative-temporal ecological characterization of the southern chernozem under the regime of the Aituar steppe site command of the Orenburg State Nature Reserve. The degree of ecological rehabilitation of chernozems in specially protected natural areas is shown. Quantitative data are provided on the supply of soil with nitrogen, phosphorus and potassium, and the content of heavy metals in the soil. Determination of the content of heavy metals and trace elements in soil samples (according to the method of Krupsky and Aleksandrova in the modification of the Central Institute of Agrochemical Services for Agriculture) was conducted in accordance with GOST 50683-94. The determination of mobile compounds of phosphorus and potassium was carried out by the method of Machigin (GOST-26205-91), organic matter by the Tyurin method (GOST 26213-9), alkaline hydrolyzable nitrogen by Kornfield. The paper presents the results of a comparison of agrochemical and ecology-toxicological studies of southern chernozem in 1996-2016. The main morphogenetic characteristics of the soil profile of the chernozem of the southern carbonate low-humus low-mass medium loamy are given. Data of morphological, physical and agrochemical properties of soils were subjected to mathematical and statistical treatment. This allowed the authors to determine morphological parameters, qualitative and quantitative characteristics of the soil and their variation within the elementary soil areas, depending on the position in the landscape and the nature of use. The data obtained indicate a positive dynamics in the provision of soil with humus and elements of mineral nutrition of plants, as well as a significant reduction in the content of heavy metals throughout the soil profile. Thus, we can talk about the natural restoration of soil fertility in conditions of a protected regime in specially protected natural areas.

scholarly journals Kestabilan Inokulan Azotobacter selama Penyimpanan pada Dua Suhu

2012 ◽  
Vol 14 (1) ◽  
pp. 52
Author(s):  
Reginawanti Hindersah ◽  
Rija Sudirja
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Root Growth ◽  
Contaminated Soil ◽  
Metal Uptake ◽  
Room Temperature ◽  
Heavy Metal Uptake ◽  
Total N ◽  
The Stability ◽  
Temperature Storage

Azotobacter might be used as biological agents in bioremediation of heavy metal-contaminated soil since this rhizobacteria produceexopolysachharides (EPS) that mobilize soil heavy metals, and phytohormones that regulate root growth. So that heavy metal uptake bythe roots could be increased. The objective of this research was to verify the stability of EPS and phytohormones in Azotobacter liquidinoculants during four months in different temperature storage. Liquid inoculants has been produced in EPS-induced media and stored in200C and room temperature (24-270C) during four months. The results showed that the better temperature storage was room temperatureinstead of 20 0C since pH, total N, and EPS and phytohormones content was relatively stable during storage.

scholarly journals The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Sri Sudewi ◽  
AMBO ALA ◽  
BAHARUDDIN ◽  
MUH. FARID
Keyword(s):  
Endophytic Bacteria ◽  
Rice Plant ◽  
Physiological Characterization ◽  
Reaction Test ◽  
Phosphate Dissolution ◽  
Activity Screening ◽  
Phosphate Solubilizing ◽  
Cultivation Techniques ◽  
Central Sulawesi ◽  
Indole 3 Acetic Acid

Abstract. Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to survive even though it is cultivated with traditional cultivation techniques. The ability to survive is suspected because this local rice plant is associated with endophytic bacteria through a variety of mechanisms including being able to produce the IAA hormone and its ability to dissolve phosphate. This study aims to find endophytic bacteria that have the potential to produce IAA and phosphate solvents through morphological and physiological characterization (Gram reaction test with 3% KOH, catalase test, hypersensitivity reaction and hemolysis activity), screening of IAA-producing bacteria qualitative and quantitative, phosphate dissolution activity on Pikovskaya medium, and quantitative using a spectrophotometer. Endophytic bacteria isolates from Gintu give the highest yields in IAA production and phosphate solubility activity, namely RKGU11 and RKGU6, respectively at 4,905 mg L-1 and 10.984 mg L-1 so that it has the potential to be used as a candidate for biofertilizer agents in developing effective sustainable agriculture, respectively, efficient and environmentally friendly.

scholarly journals State of Heavy Metals Pollution of Flooded Agricultural Land in the North Part of Republic of Srpska

2017 ◽  
Vol 17 (1) ◽  
pp. 19
Author(s):  
Tihomir Predić ◽  
Petra Nikić Nauth ◽  
Bojana Radanović ◽  
Alen Predić
Keyword(s):  
Heavy Metals ◽  
Soil Contamination ◽  
Agricultural Land ◽  
Total Content ◽  
Soil Samples ◽  
Available Phosphorus ◽  
The North ◽  
North Part ◽  
Grid Points ◽  
Phosphorus And Potassium

This paper presents the results of the research of soil contamination in flooded area located at the confluence of Bosna and Sava rivers. The sampling was performed according to a network of points Etrs89 reference European Network based on grid points of 500 m x 500 m. The total number of soil samples was 31 and 13 samples of sediment-mud. In the soil samples and sediment-mud samples various parameters were analyzed: pH H2O and 1 M KCl, humus (method by Tjurin), and the readily available phosphorus and potassium (AL method). The interpretation of total content of heavy metals in the soil was conducted according to Dutch Soil Remediation Circular (2009). The samples were analyzed for the total content of heavy metals: Pb, Cd, Cr, Ni, Zn, Cu (aqua regia digestion, AAS) and the elevated concentrations of Ni. However, Ni content in mud was from 240.5 mg/kg to 294.5 mg/kg, in soil it was from 128.9 mg/kg to 452.1 mg/kg. All mud samples were alkaline, and 79% of soil samples were alkaline and of neutral reaction. The analysis indicated that the flood in May 2014 was not caused by soil contamination with heavy metals, which however contributed to the increase of the overall content of these elements in the soil.

scholarly journals Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 761
Author(s):  
Sang-Mo Kang ◽  
Saqib Bilal ◽  
Raheem Shahzad ◽  
Yu-Na Kim ◽  
Chang-Wook Park ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Klebsiella Pneumoniae ◽  
Culture Filtrate ◽  
Catalase Activity ◽  
Endophytic Bacteria ◽  
Germination Rate ◽  
Herbicidal Activity ◽  
Dependent Manner ◽  
Evening Primrose ◽  
Indole 3 Acetic Acid

Information on the use of endophytic bacteria as a bio-herbicide for the management of weed control in agricultural fields is limited. The current study aimed to isolate endophytic bacteria from evening primroses and to screen them for their bio-herbicidal activity. Two isolated endophytic bacteria (Pantoea dispersa YNA11 and Klebsiella pneumoniae YNA12) were initially screened for citrate utilization and for indole-3-acetic acid (IAA) and catalase production. The preliminary biochemical assessment showed YNA12 as a positive strain. Ammonia, catalase, and IAA in its culture filtrate were quantified. Gas Chromatography/Mass Spectroscopy- Selective Ion Monitoring (GC/MS-SIM) analysis revealed the production of IAA by YNA12 in a time-dependent manner. YNA12 also exhibited significant ammonia-producing potential and catalase activity against hydrogen peroxide. The YNA12 culture filtrate significantly inhibited the germination rate of evening primrose seeds, resulting in a marked reduction in seedling length and biomass compared with those of the control seeds. Moreover, the culture filtrate of YNA12 significantly accelerated the endogenous abscisic acid (ABA) production and catalase activity of evening primrose seedlings. Macronutrient regulation was adversely affected in the seedlings exposed to the culture filtrate of YNA12, leading to inhibition of seed germination. The current results suggest that endophytic YNA12 may be used as a potent bio-herbicidal agent for controlling weed growth and development.

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