Effect of pyrene-induced changes in root activity on growth of Chinese cabbage (Brassica campestris L.), and the health risks caused by pyrene in Chinese cabbage at different growth stages

Background Polycyclic aromatic hydrocarbons (PAHs) pose a potential risk to ecological safety and human health. They have a range of effects on plant growth and there have been few reports on the health risks associated with ingestion of vegetable crops at different growth stages. Methodology In this study, a pot experiment in which Chinese cabbage (Brassica campestris L.) were grown in a greenhouse for 75 days was used to investigate the dose–effect relationship of pyrene with plant growth and also the exposure risk for adults of ingestion of Chinese cabbage at different growth stages. Results The results showed that low doses of pyrene (5–45 mg kg−1) promoted plant growth (20–220% and 55–97% higher than control treatment for the root biomass and shoot biomass, respectively), but significant inhibition was observed at a high dose (405 mg kg−1) (41–66% and 43–91% lower than control treatment for the root biomass and shoot biomass, respectively). High doses of pyrene reduced soil bacterial abundance and diversity during the growth of Chinese cabbage, and increased malondialdehyde (MDA) levels in the plant. The effects of pyrene on plant biomass were mainly attributed to changes in root activity induced by pyrene, as the relationship between soil pyrene concentration and biomass was similar to that between soil pyrene concentration and root activity. Furthermore, structural equation modeling analysis showed that pyrene altered growth of the vegetable by directly affecting root activity. The incremental lifetime cancer risk for adults is highest for ingestion of Chinese cabbage at the seedling stage, followed in decreasing order by the rosette stages and heading stages. Conclusions The health risk of consumers who have the possibility to ingest the Chinese cabbage planted in pyrene-contaminated soil would be decreased with the increasing growth periods. However, further studies are required to confirm the dose–effect relationship between pyrene concentration and Chinese cabbage growth on a field scale. Graphical Abstract

Calcium Affects Growth and Physiological Indices of Panax quinquefolium L.

Calcium (Ca) is necessary for plant growth and stress resistance, which are essential for the successful cultivation of Panax quinquefolium L. (American ginseng). However, information about the physiology of Ca nutrition in this species is limited. Therefore, the objective of this study was to determine the effect of Ca on the growth and physiological performance of American ginseng. Two-year-old American ginseng plants were supplemented with the following Ca concentrations [Ca2+] in a hydroponic system: 0, 160.17, 320.34, 640.68, and 961.02 mg⋅L−1. Measurements included growth biomass accumulation, chlorophyll (Chl) content and fluorescence, photosynthetic parameters, antioxidant enzyme activity, root activity, and malondialdehyde content. Biomass, stem height, leaf area, maximum photochemical efficiency, and superoxide dismutase activity peaked at [Ca2+] of 640.68 mg⋅L−1. Actual photochemical efficiency, minimum saturating irradiance, photosynthetic rate, catalase and peroxidase activities, and root activity reached their maximum at [Ca2+] of 320.34 mg⋅L−1. Stem diameter and regulated thermal energy dissipation increased with [Ca2+]. The sum of nonregulated heat dissipation and fluorescence emission and malondialdehyde content decreased to a minimum at [Ca2+] of 320.34 mg⋅L−1. The Chl content reached a maximum at [Ca2+] of 160.17 mg⋅L−1, but the Chl a/b ratio increased with [Ca2+]; the actual photochemical efficiency and photosynthetic rate reached their maximum level at Chl a/b ratios of 2.04 and [Ca2+] of 320.34 mg⋅L−1. Therefore, the optimal [Ca2+] for American ginseng growth was 320.34 mg⋅L−1. Furthermore, an appropriate increase [Ca2+] in the growth medium may improve biomass accumulation, light energy utilization efficiency, and stress resistance in American ginseng.

Influence of Planting Systems and Feeding Regimes on Dry Mass Accumulation of Cotton

Annotation: In order to increase the organic biomass in a bush, at least 15 t / ha of semi-rotted cattle manure before plowing, if possible plowed to 30 t / ha in autumn and then sowing in spring, retains the ability to absorb root activity longer, which has a positive effect on yield. reaches

Increased Carbon Dioxide by Occupants Promotes Growth of Leafy Vegetables Grown in Indoor Cultivation System

The development of various types of plant factories is central to improving agriculture. In one form, it is expanding from the existing commercial plant factories to home cultivation systems or cultivators. The plant cultivation system grafted into the living space for people produces differences in the growth of the plant depending on the lifestyle (cooling and heating, residence time, number of residents, etc.) of the resident. In this study, identical home cultivation systems that automatically adjust environmental conditions (temperature, photoperiod, light, and nutrient solution supply) other than the carbon dioxide level were set in an office and warehouse. The study confirmed how plant growth can differ depending on the amount of carbon dioxide generated by humans occupying the space. In addition, it was confirmed whether the growth of plants can be further promoted depending on the external air exchange speed by a ventilation fan even if the indoor carbon dioxide concentration is the same. Due to the nature of the cultivation system that controls the temperature, the type and speed of the fan were set to minimize heat loss in the cultivator. The airspeed from ventilation fans attached to the indoor cultivation systems of an office and warehouse was adjusted to one of three levels (0.7, 1.0, or 1.3 m·s−1). In this study with two species, Ssamchoo and Romaine, it was confirmed that the office space was significantly advantageous for the growth of Ssamchoo, especially in terms of the fresh weight, root activity, and chlorophyll content. Romaine also had a significantly higher fresh weight when grown in the office. Shoot length, leaf length, and leaf width were longer, and there were more leaves. When comparing the relative yield based on an airspeed of 1.0 m·s−1, the yield increased up to 156.9% more in the office than in the warehouse. The fan airspeed had an important influence on Ssamchoo. The higher the fan airspeed, the greater the yield, root activity, and chlorophyll. However, fan airspeed had no consistent effect on the growth tendencies of Romaine. In conclusion, carbon dioxide produced by humans occupying the space is a significant source of carbon dioxide for plants grown in the home cultivation system, although both the speed of the ventilation fan that can promote growth without heat loss and delayed growth caused by the photorespiration in a carbon dioxide-limited situation require additional experiments.

Bismuth Vanadium Oxide Can Promote Growth and Activity in Arabidopsis thaliana

The excellent properties of nanomaterials have been confirmed in many fields, but their effects on plants are still unclear. In this study, different concentrations of bismuth vanadate (BV) were added to the growth medium to analyze the growth of seedlings, including taproots, lateral roots, leaf stomata, root activity, and superoxide anion O2.- generation. Gene expression levels related to root growth were determined by quantitative PCR in Arabidopsis thaliana. The results showed that BV promoted the growth of taproots and the development of lateral roots, enhanced the length of the extension zone in roots, increased the number and size of leaf stomata and root activity, reduced the accumulation of ROS in seedlings, and changed the expression levels of genes related to polyamines or hormones. At the same time, we investigated the antibacterial activity of BV against a variety of common pathogens causing crop diseases. The results showed that BV could effectively inhibit the growth of Fusarium wilt of cotton and rice sheath blight. These results provide a new prospect for the development of nanomaterial-assisted plants, which is expected to become one of the ways to solve the problem of controlling and promoting the development of plants. At the same time, it also provides a reference for the study of the effect of BV on plants.

Effects of Long-Term Nitrogen Fertilization and Ground Water Level Changes on Soil CO2 Fluxes from Oil Palm Plantation on Tropical Peatland

A long-term study on the effect of nitrogen (N) fertilization on soil carbon dioxide (CO2) fluxes in tropical peatland was conducted to (1) quantify the annual CO2 emissions from an oil palm plantation under different N application rates and (2) evaluate the temporal effects of groundwater level (GWL) and water-filled pore space (WFPS) on soil organic carbon (SOC) and CO2 fluxes. Monthly measurement of soil CO2 fluxes using a closed chamber method was carried out from January 2010 until December 2013 and from January 2016 to December 2017 in an oil palm plantation on tropical peat in Sarawak, Malaysia. Besides the control (T1, without N fertilization), there were three N treatments: low N (T2, 31.1 kg N ha−1 year−1), moderate N (T3, 62.2 kg N ha−1 year−1), and high N (T4, 124.3 kg N ha−1 year−1). The annual CO2 emissions ranged from 7.7 ± 1.2 (mean ± SE) to 16.6 ± 1.0 t C ha−1 year−1, 9.8 ± 0.5 to 14.8 ± 1.4 t C ha−1 year−1, 10.5 ± 1.8 to 16.8 ± 0.6 t C ha−1 year−1, and 10.4 ± 1.8 to 17.1 ± 3.9 t C ha−1 year−1 for T1, T2, T3, and T4, respectively. Application of N fertilizer had no significant effect on annual cumulative CO2 emissions in each year (p = 0.448), which was probably due to the formation of large quantities of inorganic N when GWL was temporarily lowered from January 2010 to June 2010 (−80.9 to −103.4 cm below the peat surface), and partly due to low soil organic matter (SOM) quality. A negative relationship between GWL and CO2 fluxes (p < 0.05) and a positive relationship between GWL and WFPS (p < 0.001) were found only when the oil palm was young (2010 and 2011) (p < 0.05), indicating that lowering of GWL increased CO2 fluxes and decreased WFPS when the oil palm was young. This was possibly due to the fact that parameters such as root activity might be more predominant than GWL in governing soil respiration in older oil palm plantations when GWL was maintained near or within the rooting zone (0–50 cm). This study highlights the importance of roots and WFPS over GWL in governing soil respiration in older oil palm plantations. A proper understanding of the interaction between the direct or indirect effect of root activity on CO2 fluxes and balancing its roles in nutrient and water management strategies is critical for sustainable use of tropical peatland.

Foliar Arginine Application Improves Tomato Plant Growth, Yield, and Fruit Quality

Tomatoes are important for human health, and there is an urgent need to increase tomato yield and quality worldwide. Arginine is proven to be beneficial for crop storage quality and stress resistance in plants; however, its effects on tomato production and quality have not been fully investigated. This study demonstrated the effects of spray application of arginine on tomato growth, yield, and quality. Arginine treatment significantly increased the nitrogen concentration in the aboveground tomato plant parts and fruit, net photosynthetic rate, stem diameter, dry mass weight, and root activity. It is suggested that increased nitrogen accumulation following arginine application is mainly due to arginine uptake as organic nitrogen, higher expression of the LeNRT1.1 gene, and increased root activity. The increased nitrogen levels improved photosynthesis and promoted tomato plant growth. Moreover, foliar arginine application enhanced fruit size, weight, and yield. Arginine treatment had a positive effect on tomato quality as indicated by the concentrations of lycopene, vitamin C, soluble solid, soluble sugar, and titratable acids, and the sugar-acid ratio. Arginine application has potential to improve tomato production by regulating plant development and enhancing fruit yield and quality.

Competing Processes Drive the Resistance of Soil Carbon to Alterations in Organic Inputs

Protecting existing soil carbon (C) and harnessing the C sequestration potential of soils require an improved understanding of the processes through which soil organic matter accumulates in natural systems. Currently, competing hypotheses exist regarding the dominant mechanisms for soil C stabilization. Many long-standing hypotheses revolve around an assumed positive relationship between the quantity of organic inputs and soil C accumulation, while more recent hypotheses have shifted attention toward the complex controls of microbial processing and organo-mineral complexation. Here, we present the observed findings of soil response to 20 years of detrital manipulations in the wet, temperate forest of the H.J. Andrews Experimental Station. Annual additions of low-quality (high C:N content) wood litter to the soil surface led to a greater positive effect on observed mean soil C concentration relative to additions of higher-quality (low C:N content) needle litter over the 20-year study period. However, high variability in measurements of soil C led to a statistically non-significant difference in C concentration between the two treatments and the control soil. The observed soil C responses to these two addition treatments demonstrates the long timescale and potential magnitude of soil C responses to management or disturbance led changes in forest litter input composition. Detrital input reduction treatments, including cutting off live root activity and the aboveground removal of surface litter, led to relatively small, non-significant effects on soil C concentrations over the 20-year study period. Far greater negative effects on mean soil C concentrations were observed for the combined removal of both aboveground litter and belowground root activity, which led to an observed, yet also non-significant, 20% decline in soil C stocks. The substantial proportion of remaining soil C following these dramatic, long-term reductions in above- and belowground detrital inputs suggests that losses of C in these forest soils are not readily achieved over a few decades of reductions in detrital input and may require far greater periods of time or further perturbations to the environment. Further, the observed soil C responses to detrital manipulations support recent hypotheses regarding soil C stabilization, which emphasize litter quality and mineral stabilization as relevant controls over forest soil C.