A pilot study to determine whether differences exist in histochemical properties between the trapezius and extensor carpi radialis brevis muscles in women with work-related myalgia

2014 ◽  
Vol 92 (4) ◽  
pp. 315-323 ◽  
Author(s):  
Howard J. Green ◽  
Don Ranney ◽  
Margaret Burnett ◽  
Patti Galvin ◽  
Natasha Kyle ◽  
...  
Keyword(s):  
Fibre Type ◽  
Succinic Dehydrogenase ◽  
Type I ◽  
Succinic Dehydrogenase Activity ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Work Related ◽  
Fibre Area ◽  
Extensor Carpi Radialis

To investigate fibre-type abnormalities in women with work-related myalgia (WRM), tissue samples were extracted from their trapezius (TRAP) and the extensor carpi radialis brevis (ECRB) muscles and compared with healthy controls (CON). For the ECRB samples (CON, n = 6; WRM, n = 11), no differences (P > 0.05) were found between groups for any of the properties examined, namely fibre-type (I, IIA, IIX, IIAX) distribution, cross-sectional fibre area, capillary counts (CC), capillary to fibre area ratio, and succinic dehydrogenase activity. For the TRAP samples (CON, n = 6; WRM, n = 8), the only difference (P < 0.05) observed between groups was for CC (CON > WRM), which was not statistically significant (P > 0.05) when age was used a covariant. A comparison of the properties of these 2 muscles in the CON group indicated a higher (P < 0.05) and lower (P < 0.05) percentage of type I and type IIA fibres, respectively, in the TRAP as well as higher (P < 0.05) CC, which was not specific to fibre type. These preliminary results suggest that the properties employed to characterize fibre types do not differentiate CON from WRM for either the TRAP or ECRB. As a consequence, the role of inherent fibre-type differences between these muscles in the pathogenesis of WRM remains uncertain.

scholarly journals Nurses Burnout, Resilience, and Its Association With Socio-Demographic Factors During COVID-19 Pandemic

2022 ◽  
Vol 12 ◽  
Author(s):  
Majid Heidari Jamebozorgi ◽  
Ali Karamoozian ◽  
Tayebe Ilaghinezhad Bardsiri ◽  
Hojjat Sheikhbardsiri
Keyword(s):  
Negative Correlation ◽  
Maslach Burnout Inventory ◽  
Significant Negative Correlation ◽  
Cross Sectional Study ◽  
Stressful Events ◽  
Personal Accomplishment ◽  
Cross Sectional ◽  
Work Related ◽  
Socio Demographic Factors

BackgroundIn the recent pandemic, nurses have faced workload and being exposed to burnout. Resilience helps address work-related psychological problems such as stressful events and burnout. According to the roles of nurses in the healthcare system, we investigated the relationship between resiliency and burnout in nurses.Material and MethodsIn this descriptive analytical cross-sectional study, 364 nurses participated from April to June 2021. Census sampling was used to recruit participants. Maslach burnout inventory (MBI), Connor-Davidson Resiliency Scale (CDRISC), and a demographic check-list were utilized to collect data. Data analysis was done using SPSS version 22. Shapiro-Wilk, Kruskal–Wallis test, Mann–Whitney U-test, correlation analysis, and generalized linear model were applied accordingly.ResultsOverall, the findings showed that nurses had severe symptoms of burnout and a moderate level of resilience. The two domains of burnout, emotional exhaustion and personal accomplishment had a significantly negative correlation with resilience (r = −0.442, p &lt; 0.001 and r = −0.351, p = 0.03, respectively). Linear regression showed that demographic characteristics (Hospital type, ward type, gender, and overtime) were the major predictors of the 3 sub-categories of burnout. A significant negative correlation was observed between burnout and resilience highlighting the role of resilience in reducing burnout (P &lt; 0.05).ConclusionIn order to help nurses to tackle and endure burnout in pandemic times, there is a need to implement national and local policies to help them accordingly.

Personality and boredom at work: the mediating role of job crafting

2019 ◽  
Vol 24 (4) ◽  
pp. 315-330
Author(s):  
Bogdan Oprea ◽  
Dragos Iliescu ◽  
Vlad Burtăverde ◽  
Miruna Dumitrache
Keyword(s):  
Job Crafting ◽  
Negative Consequences ◽  
Cross Sectional ◽  
Content Type ◽  
Work Related ◽  
Mediating Role ◽  
Cross Sectional Design ◽  
The Individual ◽  
The Relationship

Purpose Boredom at work is associated with negative consequences, therefore it is important to investigate whether employees engage in job crafting behaviors that reduce boredom and what are the individual differences associated with these behaviors. The paper aims to discuss these issues. Design/methodology/approach A questionnaire study was designed to examine the mediating role of job crafting in the relationship between conscientiousness and emotional stability and boredom among 252 employees (Study 1) and in the relationship between Machiavellianism and psychopathy and boredom among 216 employees (Study 2). Findings The results showed that conscientiousness is negatively related to work-related boredom. This relationship is mediated by job crafting. Neuroticism and psychopathy are positively associated with boredom at work, but these relationships are not mediated by job crafting behaviors. Research limitations/implications The study was based on self-reported measures, which might raise questions of common-method bias, and the research samples contained mostly women and young employees, which raises questions about generalizability of our findings. At the same time, the cross-sectional design does not allow causal inferences. Practical implications Organizations can select employees based on their personality for jobs that predispose to boredom and give them enough autonomy to be able to craft them. Moreover, they can identify employees who need support to manage their boredom and include them in job crafting interventions. Originality/value Traditionally, boredom at work has been considered as resulting from characteristics of tasks and jobs. The findings indicate that some employees can make self-initiated changes to their work in order to reduce their boredom and possibly its negative consequences.

Rat diaphragm during postnatal development. I. Changes in distribution of muscle fibre type and in oxidative potential

1996 ◽  
Vol 8 (3) ◽  
pp. 391 ◽  
Author(s):  
MD Fratacci ◽  
M Levame ◽  
A Rauss ◽  
H Bousbaa ◽  
G Atlan
Keyword(s):  
Muscle Fibre ◽  
Fibre Type ◽  
Oxidative Capacity ◽  
Cross Sectional Area ◽  
Fibre Types ◽  
Type I ◽  
Sectional Area ◽  
Rat Diaphragm ◽  
Oxidative Potential ◽  
Cross Sectional

The changes occurring in the histochemical characteristics of the rat diaphragm during the postnatal period were examined. Fibre-type distribution, fibre oxidative capacity, i.e. succinate-dehydrogenase (SDH) activity, and cross-sectional area were compared in the costal (COS) and crural (CRU) regions, and across their abdominal and thoracic surfaces. The proportions of type I and IIb fibres in both COS and CRU increased with age, while the proportion of type IIa fibres progressively decreased. For COS, fibre distribution was homogeneous over the entire muscle and did not change after 4 weeks. For CRU, it was heterogeneous with a higher proportion of type I fibres on the thoracic surface as from the first week. All fibre types significantly increased in cross-sectional area between 1 and 8 weeks, with no significant differences in COS and CRU. Mean SDH activity did not differ between COS and CRU or across the muscles. Mean SDH activities-were low and identical in all fibre types at birth, and then increased, peaking at the 6th week in type I and IIa fibres. When total muscle fibre oxidative capacity was calculated from an index including fibre-type proportion, cross-sectional area and mean SDH activity, it was significantly higher at 1 than at 8 weeks after birth; this might have functional implications for the newborn.

scholarly journals Coxiella burnetii seroprevalence in primiparous and multiparous ewes from southern Australia: a cross-sectional study

2021 ◽  
Author(s):  
Thomas Clune ◽  
Amy Lockwood ◽  
Serina Hancock ◽  
Andrew N. Thompson ◽  
Mieghan Bruce ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Reproductive Performance ◽  
Q Fever ◽  
Cross Sectional Study ◽  
Molecular Diagnostic ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Natural Exposure ◽  
Reproductive Disease

Abstract Coxiella burnetii can cause reproductive disease in sheep and zoonotic Q-fever infections in humans. The role of infectious diseases including coxiellosis in causing poorer reproductive performance of primiparous ewes is not well studied. The aim of this study was to determine if natural exposure to C. burnetii is associated with poor reproductive performance of primiparous ewes and compare seroprevalence of primiparous and multiparous ewes. Coxiella burnetii seroprevalence was 0.08% (95% confidence interval 0.01, 0.36) in primiparous ewes and 0.36% (0.07, 1.14) in mature ewes. Coxiella burnetii was not detected in tissue samples from aborted or stillborn lambs using molecular diagnostic tests (qPCR). These findings suggest that C. burnetii infection was unlikely to be an important contributor to abortion and perinatal mortalities observed for primiparous ewes, and exposure to C. burnetii was not widespread in ewes on farms located over wide geographical region of southern Australia.

Capillarity and fiber types in the cremaster muscle of rat and hamster

1983 ◽  
Vol 245 (2) ◽  
pp. H368-H374 ◽  
Author(s):  
I. H. Sarelius ◽  
L. C. Maxwell ◽  
S. D. Gray ◽  
B. R. Duling
Keyword(s):  
Muscle Fiber ◽  
Fiber Type ◽  
Succinic Dehydrogenase ◽  
Capillary Density ◽  
Type I ◽  
Fiber Types ◽  
Cremaster Muscle ◽  
Muscle Area ◽  
Cross Sectional

We determined muscle fiber type and capillarity in cremaster muscle samples from rats and hamsters of different ages. Histochemical estimation of oxidative capacity was made from the activity of either nicotinamide dinucleotide tetrazolium reductase (NADH-TR) or succinic dehydrogenase (SDH), and fibers were termed fast or slow from myofibrillar ATPase activity. Fibers were classified as type I (low ATPase, high NADH-TR/SDH), type IIa (high ATPase, high SDH/NADH-TR), type IIb (high ATPase, low SDH/NADH-TR), or type IIc (no acid reversal of ATPase, high NADH-TR). Type IIb fibers accounted for 60-80% of the muscle area in both species at all ages. The principal change with maturation was muscle fiber hypertrophy. Mean cross-sectional fiber area increased from 488 +/- 70 (SE) and 453 +/- 19 micron2 in young hamsters and rats, respectively, to 1,255 +/- 99 and 1,540 +/- 101 micron2 in adults. Capillary density (no. of capillaries/mm2 tissue) paralleled fiber hypertrophy; it decreased significantly with maturation from 684 +/- 60 (SE) to 228 +/- 26/mm2 in hamsters and from 341 +/- 15 to 213 +/- 15/mm2 in rats. In vitro estimates of capillary density are compared with previously obtained in vivo data (31), and sources of error are identified. We conclude that reported differences in microvascular function in the cremaster muscle in vivo during maturation or between species cannot be ascribed to changes in muscle composition.

Strength, skeletal muscle composition, and enzyme activity in multiple sclerosis

1997 ◽  
Vol 83 (6) ◽  
pp. 1998-2004 ◽  
Author(s):  
J. A. Kent-Braun ◽  
A. V. Ng ◽  
M. Castro ◽  
M. W. Weiner ◽  
D. Gelinas ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Skeletal Muscle ◽  
Enzyme Activity ◽  
Succinic Dehydrogenase ◽  
Morphological Characteristics ◽  
Cross Sectional Area ◽  
Type I ◽  
Sectional Area ◽  
Cross Sectional ◽  
Muscle Composition

Kent-Braun, J. A., A. V. Ng, M. Castro, M. W. Weiner, D. Gelinas, G. A. Dudley, and R. G. Miller. Strength, skeletal muscle composition and enzyme activity in multiple sclerosis. J. Appl. Physiol. 83(6): 1998–2004, 1997.—This study examined functional, biochemical, and morphological characteristics of skeletal muscle in nine multiple sclerosis (MS) patients and eight healthy controls in an effort to ascertain whether intramuscular adaptations could account for excessive fatigue in this disease. Analyses of biopsies of the tibialis anterior muscle showed that there were fewer type I fibers (66 ± 6 vs. 76 ± 6%), and that fibers of all types were smaller (average ↓26%) and had lower succinic dehydrogenase (SDH; average ↓40%) and SDH/α-glycerol-phosphate dehydrogenase (GPDH) but not GPDH activities in MS vs. control subjects, suggesting that muscle in this disease is smaller and relies more on anaerobic than aerobic-oxidative energy supply than does muscle of healthy individuals. Maximal voluntary isometric force for dorsiflexion was associated with both average fiber cross-sectional area ( r = 0.71, P = 0.005) and muscle fat-free cross-sectional area by magnetic resonance imaging ( r = 0.80, P < 0.001). Physical activity, assessed by accelerometer, was associated with average fiber SDH/GPDH ( r = 0.78, P = 0.008). There was a tendency for symptomatic fatigue to be inversely associated with average fiber SDH activity ( r = −0.57, P = 0.068). The results of this study suggest that the inherent characteristics of skeletal muscle fibers per se and of skeletal muscle as a whole are altered in the direction of disuse in MS. They also suggest that changes in skeletal muscle in MS may significantly affect function.

Time-dependent effects of short-term training on muscle metabolism during the early phase of exercise

2009 ◽  
Vol 297 (5) ◽  
pp. R1383-R1391 ◽  
Author(s):  
H. J. Green ◽  
E. Bombardier ◽  
M. E. Burnett ◽  
I. C. Smith ◽  
S. M. Tupling ◽  
...  
Keyword(s):  
Citrate Synthase ◽  
Vastus Lateralis ◽  
Succinic Dehydrogenase ◽  
Muscle Metabolism ◽  
Short Term ◽  
Oxidative Potential ◽  
Tissue Samples ◽  
Nonsteady State ◽  
Pyruvate Ratio

In this study, we investigated the hypothesis that the metabolic adaptations observed during steady-state exercise soon after the onset of training would be displayed during the nonsteady period of moderate exercise and would occur in the absence of increases in peak aerobic power (V̇o2peak) and in muscle oxidative potential. Nine untrained males [age = 20.8 ± 0.70 (SE) yr] performed a cycle task at 62% V̇o2peak before (Pre-T) and after (Post-T) training for 2 h/day for 5 days at task intensity. Tissue samples extracted from the vastus lateralis at 0 min (before exercise) and at 10, 60, and 180 s of exercise, indicated that at Pre-T, reductions ( P < 0.05) in phosphocreatine and increases ( P < 0.05) in creatine, inorganic phosphate, calculated free ADP, and free AMP occurred at 60 and 180 s but not at 10 s. At Post-T, the concentrations of all metabolites were blunted ( P < 0.05) at 60 s. Training also reduced ( P < 0.05) the increase in lactate and the lactate-to-pyruvate ratio observed during exercise at Pre-T. These adaptations occurred in the absence of change in V̇o2peak (47.8 ± 1.7 vs. 49.2 ± 1.7 ml·kg−1·min−1) and in the activities (mol·kg protein−1·h−1) of succinic dehydrogenase (3.48 ± 0.21 vs. 3.77 ± 0.35) and citrate synthase (7.48 ± 0.61 vs. 8.52 ± 0.65) but not cytochrome oxidase (70.8 ± 5.1 vs. 79.6 ± 6.6 U/g protein; P < 0.05). It is concluded that the tighter metabolic control observed following short-term training is initially expressed during the nonsteady state, probably as a result of increases in oxidative phosphorylation that is not dependent on changes in V̇o2peak while the role of oxidative potential remains uncertain.

Excitation–contraction coupling properties in women with work-related myalgia: a preliminary study

2014 ◽  
Vol 92 (6) ◽  
pp. 498-506 ◽  
Author(s):  
Howard J. Green ◽  
Don Ranney ◽  
Margaret Burnett ◽  
Patti Galvin ◽  
Natasha Kyle ◽  
...  
Keyword(s):  
Potential Role ◽  
Healthy Controls ◽  
Β Subunit ◽  
Excitation Contraction Coupling ◽  
Contraction Coupling ◽  
Work Related ◽  
Preliminary Study ◽  
Coupling Processes ◽  
Extensor Carpi Radialis

We investigated the potential role of selected excitation–contraction coupling processes in females with work-related myalgia (WRM) by comparing WRM with healthy controls (CON) using tissue from extensor carpi radialis brevis (ECRB) and trapezius (TRAP) muscles. For the ECRB, age (mean ± SE) was 29.6 ± 3.5 years for CON (n = 9) and 39.2 ± 2.8 years for WRM (n = 13), while for the TRAP, the values were 26.0 ± 2.1 years for CON (n = 7) and 44.6 ± 2.9 years for WRM (n = 11). For the sarcoplasmic reticulum (SR) of the ECRB, WRM displayed concentrations (nmol·(mg protein)−1·min−1) that were lower (P < 0.05) for Total (202 ± 4.4 vs 178 ± 7.1), Basal (34 ± 1.6 vs 30.1 ± 1.3), and maximal Ca2+-ATPase activity (Vmax, 168 ± 4.9 vs 149 ± 6.3), and Ca2+-uptake (5.06 ± 0.31 vs 4.13 ± 0.29), but not SERCA1a and SERCA2a isoforms, by comparison with CON. When age was incorporated as a co-variant, Total, Basal, and Ca2+-uptake remained different from CON (P < 0.05), but not Vmax (P = 0.13). For TRAP, none of the ATPase properties differed between groups (P > 0.05) either before or following adjustment for age. No differences (P > 0.05) were observed between the groups for Ca2+-release in the SR for either TRAP or ECRB. Similarly, no deficiencies, regardless of muscle, were noted for either the Na+–K+-ATPase content or the α and β subunit isoform distribution in WRM. This preliminary study provides a basis for further research, with expanded numbers, investigating the hypothesis that abnormalities in SR Ca2+-regulation are involved in the cellular etiology of WRM.

Can increases in capillarization explain the early adaptations in metabolic regulation in human muscle to short-term training?

2012 ◽  
Vol 90 (5) ◽  
pp. 557-566 ◽  
Author(s):  
Howard J. Green ◽  
Margaret Burnett ◽  
Helen Kollias ◽  
Jing Ouyang ◽  
Ian Smith ◽  
...  
Keyword(s):  
Fibre Type ◽  
Metabolic Regulation ◽  
Vastus Lateralis ◽  
Succinic Dehydrogenase ◽  
Capillary Density ◽  
Peak Oxygen Consumption ◽  
Short Term ◽  
Oxidative Potential ◽  
Fibre Area ◽  
Fibre Type Distribution

To investigate the hypothesis that increases in fibre capillary density would precede increases in oxidative potential following training onset, tissue was extracted from the vastus lateralis prior to (0 days) and following 3 and 6 consecutive days of submaximal cycle exercise (2 h·day–1). Participants were untrained males (age = 21.4 ± 0.58 years; peak oxygen consumption = 46.2 ± 1.6 mL·kg–1·min–1; mean ± standard error (SE)). Tissue was assessed for succinic dehydrogenase activity (SDH) by microphotometry and indices of capillarization based on histochemically assessed area and capillary counts (CC) in specific fibre types. Three days of training (n = 13) resulted in a generalized decrease (p < 0.05) in fibre area (–14.2% ± 3.0%; mean ± SE) and increase (p < 0.05) in CC/Area (20.4% ± 2.7%) and no change in either CC or SDH activity. Following 6 days of treatment (n = 6), increases (p < 0.05) in CC (18.2% ± 4.2%), CC/Area (28.9% ± 3.2%), and SDH activity (22.9% ± 6.0%) occurred that was not specific to major fibre type. No changes in either fibre area or fibre-type distribution were observed with additional training. We conclude that increases in angiogenic-based capillary density and oxidative potential occur coincidentally following training onset, while increases in capillary density, mediated by reductions in fibre area, represent an initial isolated response, the significance of which may be linked to the metabolic alterations that also result.

scholarly journals Work-Related Satisfaction among Clinicians Working at Inpatient Treatment Facilities for Substance Use Disorder: The Role of Recovery Orientation

2021 ◽  
Vol 18 (14) ◽  
pp. 7423
Author(s):  
Dagny Adriaenssen Johannessen ◽  
Trond Nordfjærn ◽  
Amy Østertun Geirdal
Keyword(s):  
Substance Use ◽  
Psychosocial Factors ◽  
Substance Use Disorder ◽  
Survey Study ◽  
Cross Sectional ◽  
Staff Members ◽  
Work Related ◽  
Recovery Orientation ◽  
Treatment Facilities

Several psychosocial factors have been suggested as facilitators of change among inpatients treated for substance use disorder (SUD). Research suggests that staff members are also influenced by the practice in which they are involved, and by contextual psychosocial factors at their treatment facilities. This cross-sectional questionnaire survey study was conducted to investigate the role of recovery-orientated interventions in describing work-related satisfaction among clinicians at inpatient SUD treatment facilities. The respondents (n = 407) rated items indicating work-related satisfaction and the degree of recovery orientation at their treatment facilities. The main findings of two block regression analyses indicated that clinicians’ work-related satisfaction was positively influenced by inpatients’ opportunities to pursue their goals and choices, and negatively influenced by inpatient involvement. The change in clinicians’ work-related satisfaction could not be described by the degree of individually tailored and varied interventions at the treatment facility. Clinicians should be supported and involved in the process of implementing measures to increase inpatient involvement in the treatment programmes, and treatment measures that enable inpatients to pursue their goals and choices should be enhanced. The findings of this and previous studies indicate that a recovery-oriented framework promotes clinicians’ work-related satisfaction and has an enabling influence on both inpatients and clinicians.

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