scholarly journals Coxiella burnetii seroprevalence in primiparous and multiparous ewes from southern Australia: a cross-sectional study

2021 ◽  
Author(s):  
Thomas Clune ◽  
Amy Lockwood ◽  
Serina Hancock ◽  
Andrew N. Thompson ◽  
Mieghan Bruce ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Reproductive Performance ◽  
Q Fever ◽  
Cross Sectional Study ◽  
Molecular Diagnostic ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Natural Exposure ◽  
Reproductive Disease

Abstract Coxiella burnetii can cause reproductive disease in sheep and zoonotic Q-fever infections in humans. The role of infectious diseases including coxiellosis in causing poorer reproductive performance of primiparous ewes is not well studied. The aim of this study was to determine if natural exposure to C. burnetii is associated with poor reproductive performance of primiparous ewes and compare seroprevalence of primiparous and multiparous ewes. Coxiella burnetii seroprevalence was 0.08% (95% confidence interval 0.01, 0.36) in primiparous ewes and 0.36% (0.07, 1.14) in mature ewes. Coxiella burnetii was not detected in tissue samples from aborted or stillborn lambs using molecular diagnostic tests (qPCR). These findings suggest that C. burnetii infection was unlikely to be an important contributor to abortion and perinatal mortalities observed for primiparous ewes, and exposure to C. burnetii was not widespread in ewes on farms located over wide geographical region of southern Australia.

Seroprevalence of Coxiella burnetii in sheep flocks in Kaduna State, Northwestern Nigeria

2021 ◽  
Author(s):  
Shuaibu Gidado Adamu ◽  
Junaidu Kabir ◽  
Jarlath Udo Umoh ◽  
Mashood Abiola Raji
Keyword(s):  
Risk Factors ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Jugular Vein ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sectional Study ◽  
High Seroprevalence ◽  
Cross Sectional ◽  
Significant Difference

Abstract A cross-sectional study was carried out to determine the seroprevalence and risk factors of Q fever in sheep in the northern part of Kaduna State, Nigeria. This study aimed to determine Coxiella burnetii infection and its risk factors in sheep in Kaduna State. A total of 400 blood samples consisting of 259 samples from females and 141 from males were aseptically collected from the jugular vein of sheep from flocks in Kaduna State. The sera obtained were screened for Q fever using an indirect enzyme-linked immunosorbent assay (iELISA). The obtained data were analysed to determine whether there is a relationship between sex, age, and the animals tested. The analysis revealed that 8.0% of the sera was seropositive by iELISA. There was no significant difference in Q fever seropositivity in the study area according to the sex of sheep (P > 0.05). There was a statistically significant difference (P < 0.05) in Q fever seropositivity according to the age of sheep. This study indicated a high seroprevalence of Q fever mainly among female animals and older sheep. Further studies are required to determine the epizootiology of Q fever in the study area more precisely.

scholarly journals Seroreactivity and Risk Factors Associated with Coxiella burnetii Infection among Cattle Slaughterhouse Workers in South Korea

2018 ◽  
Vol 15 (10) ◽  
pp. 2264 ◽  
Author(s):  
Ji-Hyuk Park ◽  
Seon Hwang ◽  
Dilaram Acharya ◽  
Seung Lee ◽  
Kyu Hwang ◽  
...  
Keyword(s):  
Risk Factors ◽  
South Korea ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Close Contact ◽  
Cross Sectional Study ◽  
Immunofluorescence Assay ◽  
Cross Sectional ◽  
Factors Associated ◽  
Related Risk

Q fever, caused by Coxiella burnetii, is a zoonotic disease that is an occupational hazard to people who work in close contact with animals or their carcasses. A nationwide serologic study among cattle slaughterhouse workers who were presumed to be at risk of having C. burnetii infection in South Korea was performed to investigate the seroreactivity of C. burnetii infection and identify related risk factors. Out of 1017 cattle slaughterhouse workers in South Korea, 923 (90.8%) participated in this cross-sectional study. Samples were tested for immunoglobulin G (IgG) and M (IgM) antibodies against phase II C. burnetii via indirect immunofluorescence assay. The overall seroreactivity, defined as IgG or IgM antibody titer cutoffs ≥1:16, was 9.1% (84/923). Additionally, a significant association was found between the seroreactivity of C. burnetii infection and performing carcass evisceration work (odds ratio, 2.36; 95% confidence interval, 1.39–4.03) in multivariate analysis. To diminish C. burnetii infection, cattle slaughterhouse workers need to take precautions during the evisceration process.

Detection of Human Papillomavirus and Adenovirus in Benign and Malignant Lesions of the Larynx

2009 ◽  
Vol 141 (2) ◽  
pp. 276-281 ◽  
Author(s):  
Andrea Gallo ◽  
Anna Marta Degener ◽  
Giulio Pagliuca ◽  
Alessandra Pierangeli ◽  
Federica Bizzoni ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cross Sectional Study ◽  
Experimental Medicine ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Viral Genomes ◽  
Pathology Section ◽  
Malignant Lesions ◽  
University Of Rome

OBJECTIVE: To investigate the role of human papillomavirus (HPV) and adenovirus (AdV) infections in the oncogenesis of squamous cell carcinomas of the larynx and of laryngeal dysplasia. STUDY DESIGN: Cross-sectional study with planned data collection. SETTING: Department of Otorhinolaryngology G. Ferreri and Department of Experimental Medicine and Pathology-Section of Virology, Sapienza University of Rome. SUBJECTS AND METHODS: Biopsy samples were taken from 68 patients with benign and malignant lesions of the larynx. All tissue samples were analyzed by means of polymerase chain reaction with two groups of primers for HPV and with a pair of primers for AdV. RESULTS: All cases of carcinomas and dysplasia as well as all control cases were negative for both viruses. Four of the five cases of laryngeal papillomas were positive for only HPV, confirming the role of these viral types in the origin of papillomas. CONCLUSION: The absence of viral genomes in laryngeal carcinomas as in the other cases studied suggests the existence of other factors that play a more important role than viral infection in the carcinogenesis of these lesions.

scholarly journals Q Fever: Seroprevalence, Risk Factors in Slaughter Livestock and Genotypes of Coxiella burnetii in South Africa

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 258
Author(s):  
Maruping Mangena ◽  
Nomakorinte Gcebe ◽  
Rian Pierneef ◽  
Peter N. Thompson ◽  
Abiodun A. Adesiyun
Keyword(s):  
Risk Factors ◽  
South Africa ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Farm Workers ◽  
Reproductive Disorders ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Associated Risk Factors ◽  
Game Animals

Q fever is a neglected zoonosis in South Africa, causing significant losses in livestock and game animals through reproductive disorders. However, there are limited studies on the extent of Coxiella burnetii infections in livestock in South Africa. Further, there is also lack of knowledge about the types of C. burnetii strains that are currently circulating in the country. Therefore, a cross-sectional, abattoir-based study was conducted to determine the seroprevalence of C. burnetii and associated risk factors, and to characterize C. burnetii strains from slaughter livestock at red meat abattoirs in Gauteng, South Africa. Of the 507 animals tested, 6.9% (95% CI: 4.9–9.5%) were positive for antibodies against C. burnetii. The seroprevalence was 9.4% (31/331) in cattle, 4.3% (3/69) in sheep, and 0.9% (1/107) in pigs. Out of the 63 tissue samples from 35 seropositive animals including material from two sheep aborted fetuses from Mangaung district (Free State province), 12.7% (8/63) tested positive by IS1111 PCR. Genotyping of the eight PCR-positive tissues from eight animals by MLVA revealed two novel genotypes, not available in Coxiella MLVA databases. It is concluded that slaughter animals pose a risk of exposing abattoir and farm workers to C. burnetii in South Africa.

scholarly journals Coxiella burnetii infection in humans: to what extent do cattle in infected areas free from small ruminants play a role?

2020 ◽  
Vol 148 ◽  
Author(s):  
M. Pouquet ◽  
N. Bareille ◽  
R. Guatteo ◽  
L. Moret ◽  
F. Beaudeau
Keyword(s):  
Coxiella Burnetii ◽  
Blood Donors ◽  
Q Fever ◽  
Adult Population ◽  
Small Ruminants ◽  
Risk Groups ◽  
Cross Sectional Study ◽  
Immunofluorescence Assay ◽  
Cross Sectional ◽  
General Adult Population

Abstract Domestic ruminants (cattle, goats and sheep) are considered to be the main reservoirs for human Coxiella burnetii infection. However, there is still a need to assess the specific contribution of cattle. Indeed, most seroprevalence studies in humans were carried out in areas comprising both cattle and small ruminants, the latter being systematically implicated in human Q fever outbreaks. Therefore, we conducted a cross-sectional study in areas where C. burnetii infection in cattle was endemic, where the density of cattle and small ruminant farms were respectively high and very low. The aim was to estimate the seroprevalence rates among two occupational (cattle farmers and livestock veterinarians), and one non-occupational (general adult population) risk groups. Sera were collected in 176 cattle farmers, 45 veterinarians and 347 blood donors, and tested for phase I and II antibodies using immunofluorescence assay. Seroprevalence rates were 56.3% among cattle farmers, 88.9% among veterinarians and 12.7% among blood donors. This suggests that a specific risk for acquiring C. burnetii infection from cattle in endemically infected areas exists, mainly for occupational risk groups, but also for the general population. Further research is needed to identify risk factors for C. burnetii infection in humans in such areas.

scholarly journals Seroprevalence of Q Fever in Lorestan Province Veterinary Staff Using IFA Method

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Zahra Soleimani ◽  
Amin Jaydari
Keyword(s):  
Coxiella Burnetii ◽  
Work Experience ◽  
Q Fever ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
High Seroprevalence ◽  
Cross Sectional ◽  
Treatment And Prevention ◽  
Significant Relationships ◽  
History Of

Background: Q fever is caused by the bacterium Coxiella burnetii. To date, no studies have been conducted on the rate of Q fever infection among the veterinary staff in Lorestan province. Objectives: The present study aimed to investigate the seroprevalence of infection in the veterinary staff. Methods: This cross-sectional study was conducted from April to July 2019 on 92 samples collected randomly from Lorestan province on veterinary staff who had a history of contact with livestock and were tested via indirect immunofluorescence kit. Results: In this study, 77 (83/69%) samples were positive, and 15 (16/3%) samples were negative. There were no statistically significant relationships between residence, contact with livestock, and work experience. Conclusions: According to the results of this study, the high seroprevalence of a Q fever was observed among Veterinary staff, which further reveals the necessitates of diagnosis, treatment, and prevention of the disease in order to prevent unwanted complications in people in contact with livestock.

Role of Sildenafil in the Treatment of Persistent Pulmonary Hypertension of Newborn (PPHN) - Should it Be Withdrawn from Market of Bangladesh due to Threat of being Misuses

2020 ◽  
Vol 35 (2) ◽  
pp. 100-104
Author(s):  
Maksudur Rahman ◽  
Mohammad Abdullah Al Mamun ◽  
MAK Azad Chowdhury ◽  
Abu Sayeed Munsi
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cardiac Intensive Care Unit ◽  
Cross Sectional ◽  
Cardiac Intensive Care ◽  
Hospital Protocol

Background: Recently it has been apprehended that sildenafil, a drug which has been successfully using in the treatment of PPHN and erectile dysfunction in adult, is going to be withdrawn from the market of Bangladesh due to threat of its misuses. Objective: The aim of this study was to see the extent of uses of sildenafil in the treatment of PPHN and importance of availability of this drugs in the market inspite of its probable misuses. Methods: This cross sectional study was conducted in neonatal intensive care unit (NICU), special baby care unit (SCABU) and cardiac intensive care unit (CICU) of Dhaka Shishu (Children) Hospital from June, 2017 to May 2018. Neonates with PPHN were enrolled in the study. All cases were treated with oral sildenefil for PPHN along with others management according to hospital protocol. Data along with other parameters were collected and analyzed. Results: Total 320 patients with suspected PPHN were admitted during the study period. Among them 92 (29%) cases had PPHN. Male were 49(53 %) cases and female were 43(47%) cases. Mean age at hospital admission was 29.7±13.4 hours. Based on echocardiography,13(14%) cases had mild, 38 (41%) cases moderate and 41(45%) cases severe PPHN. Mean duration of sildenafil therapy was 11.9±7.1 days. Improved from PPHN were 83 (90%) cases. Mortality was 10% (9). Conclusion: In this study it was found that the incidence of PPHN is 29% among the suspected newborns. Sildenafil is successfull in improving the oxygenation of PPHN and to decrease the mortality of neonates. DS (Child) H J 2019; 35(2) : 100-104

Role of Fibrinolytic Therapy in Empyema Thoracis – A Hospital Based one year Cross Sectional Study

2012 ◽  
Vol 2 (2) ◽  
pp. 345-350
Author(s):  
Dr. Girish.L Dandagi ◽  
◽  
Venkat kalyana kumar. P ◽  
Dr. Dr.Isaac Mathew ◽  
Dr. Dr.G S Gaude Dr. Dr.G S Gaude
Keyword(s):  
Cross Sectional Study ◽  
Fibrinolytic Therapy ◽  
Empyema Thoracis ◽  
Sectional Study ◽  
Cross Sectional ◽  
One Year

Breastfeeding practices among mothers of infants in Peringara Panchayat in Pathanamthitta District

2018 ◽  
Vol 3 (01) ◽  
Author(s):  
Aswathy S. ◽  
Lakshmi M. K.
Keyword(s):  
Exclusive Breastfeeding ◽  
Cross Sectional Study ◽  
Community Based ◽  
Chi Square ◽  
Cross Sectional ◽  
Breastfeeding Practices ◽  
Chi Square Test ◽  
Type Of Delivery ◽  
Initiation Of Breastfeeding

The study was aimed to assess the breastfeeding practices among mothers of infants in Peringara Gramapanchayat in Kerala. Study was a community based cross-sectional study among mothers of infants in Peringara gramapanchayat using a pretested questionnaire. 142 breastfeeding mothers of infants in Peringara gramapanchayat were studied and mothers who were not present at home during the study were excluded from the study. Study period consisted of 18 days between December 2015 and January 2016. Study variables includes type of delivery, initiation of breastfeeding, breastfeeding practices and role of ASHAs in promoting good breastfeeding practices. Statistical analysis was done using Pearson’s Chi-square test and T test. The study found that exclusive breastfeeding has been done by 68.3% of mothers. There is no practice of giving pre-lacteal feed, 95.8% of mothers have given colostrum to the new born. Statistically significant association was found between the type of delivery and time of initiation of breastfeeding (p less than 0.05). Time of initiation of breastfeeding was prolonged in case of Caesarean section. 49.3% of mothers have breastfed the baby within one hour. 55.6% of mothers were informed about importance of breastfeeding by ASHAs and only 20.4% of mothers were informed about period of exclusive breastfeeding and period of complimentary feeding by ASHAs.

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