Protective effects of combined β-caryophyllene and silymarin against ketoprofen-induced hepatotoxicity in rats

2016 ◽  
Vol 94 (7) ◽  
pp. 739-744 ◽  
Author(s):  
Mohamed Elsayed Kelany ◽  
Mohamed Abdelmohsen Abdallah
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Liver Toxicity ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Protective Effects ◽  
Total Antioxidant ◽  
Liver Changes

Ketoprofen (Ket), widely utilized in treatment of many inflammatory disorders, is found to induce liver toxicity especially with overdose. This study aimed to evaluate the possible protective effects of concomitant β-caryophyllene (Cary) and silymarin (Sily) against Ket-induced hepatotoxicity in rats. Forty adult male albino rats were divided into 5 groups (each n = 8): the control group received distilled water for 6 weeks; the Ket-treated group received distilled water for 5 weeks and Ket in a dose of 8 mg·kg−1·day−1 p.o. for the 6th week; the Cary + Ket treated group received Cary in a dose of 200 mg·kg−1·day−1 orally for 6 weeks and Ket for the 6th week; the Sily + Ket treated group received Sily in the dose of 150 mg·kg−1·day−1 for 6 weeks and Ket for the 6th week; and the Cary + Sily + Ket treated group received Sily and Cary for 6 weeks and Ket for the 6th week. At end of the experiment, serum ALT, AST, and albumin and liver total antioxidant capacity (t.TAC) and malondialdehyde (t.MDA) were measured in all rats. Ket increased serum ALT and AST and t.MDA and decreased t.TAC. Cary and Sily improved these changes. Combined Cary and Sily restored these liver changes to nearly normal. Combined Cary and Sily is hepatoprotective, with the ability to scavenge oxidants against Ket-induced hepatotoxicity in rats.

scholarly journals Radioprotective and anti-diabetic effects of Costus speciosus and carnosine

2020 ◽  
Vol 19 (1) ◽  
pp. 121-127
Author(s):  
Ebtisam A. Marzook ◽  
Fawzy A. Marzook ◽  
Ahmed E. Abd El Moneim
Keyword(s):  
Gamma Radiation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Natural Antioxidants ◽  
Serum Levels ◽  
Serum Glucose ◽  
Control Group ◽  
Albino Rats ◽  
Costus Speciosus ◽  
Total Antioxidant

Purpose: To evaluate the possible radioprotective effect of Costus speciosus and carnosine as natural antioxidants in order to control the  hyperglycemia developed in male albino rats exposed to acute oxidative stress induced by gamma radiation. Methods: Twenty-eight adult male albino rats were divided into four groups. The first group was taken as a control group, while the three other groups were exposed to Ɣ irradiation at a single 7.5 Gy dose. Furthermore, the rats in the second and third groups were i.p. injected with Costus speciosus root powder and carnosine, respectively. On the 3rd day, after irradiation, the serum levels of glucose, insulin, C peptide, copper, iron, calcium, total antioxidant capacity (TAC) and malondialdehyde (MDA) were measureded. Results: The results revealed that exposure to Ɣ irradiation induced significant increases in serum glucose, iron, and malondialdehyde. However, the levels of serum calcium, copper, total antioxidant capacity and insulin significantly decreased (p < 0.05). A significant decrease was observed in Cpeptide in the exposed group, compared to control group. All the test parameters indicate improvement after treatment with Costus speciosus and carnosine (p < 0.05). Conclusion: Costus speciosus and carnosine ameliorate the effect of gamma radiation, indicating their role as antidiabetic agents and  radioprotectors; however, Costus speciosus was critically more efficient than carnosine. Keywords: Costus speciosus, Carnosine, Diabetes, Insulin, Gamma radiation protection

Protective effects of thymoquinone against methotrexate-induced testicular injury

2010 ◽  
Vol 30 (8) ◽  
pp. 897-903 ◽  
Author(s):  
Ahmet Gökçe ◽  
Suleyman Oktar ◽  
Ahmet Koc ◽  
Zafer Yonden
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Nigella Sativa ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Protective Effects ◽  
Left Testis ◽  
Total Antioxidant

Thymoquinone is the major active component derived from Nigella sativa. Methotrexate is a folic acid antagonist widely used in clinic. Aim of this study was to investigate the possible protective role of thymoquinone on testicular toxicity of methotrexate. Experiments were performed on male C57BL/6 mice (6 weeks old, 20 ± 2 g). The animals were divided into four groups with six mice in each group. Equivalent volumes of saline were injected intraperitoneally (i.p.) in the control group. In the thymoquinone group, mice received thymoquinone i.p. with a dose of 10 mg/kg/day for 4 days. Mice in the methotrexate group received single dose of methotrexate i.p., with a dose of 20 mg/kg. Finally, in the methotrexate plus thymoquinone group, in the first and the following 3 days after methotrexate administration, thymoquinone was injected with a dose of 10 mg/kg/day, i.p. At the end of the experiment, the left testis was quickly removed and divided into two parts for histological examination and biochemical analysis. Methotrexate alone increased total antioxidant capacity and myeloperoxidase activity compared to the controls. Thymoquinone treatment decreased total antioxidant capacity and prevented the increase in the myeloperoxidase activity. Light microscopy showed in mice that receiving methotrexate resulted in interstitial space dilatation, edema, severe disruption of the seminiferous epithelium and reduced diameter of the seminiferous tubules. Administration of thymoquinone reversed histological changes of methotrexate significantly. We suggest that thymoquinone use may decrease the destructive effects of methotrexate on testicular tissue of patients using this agent.

scholarly journals Ameliorating Effect of L-arginine and Insulin on Streptozotocin-induced Adrenal Gland Injury in Albino Rats

1969 ◽  
Vol 11 (3) ◽  
pp. 162-168
Author(s):  
Yasmeen Mahar ◽  
Alisha Qamar ◽  
lnayatullah ◽  
Sarwath Fatimee ◽  
Mohammad Fawad Saeeduddin ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Treated Group ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Endocrine Gland ◽  
Protective Effects ◽  
Frozen Sections ◽  
Group B

Background:Use of dietary supplements to treat illnesses has increasedtremendously in recentyears.Adrenal gland is one ofthemost commonly damaged endocrine gland in the body, not only by chemical or radiation injuries, but also as a result of differenttypes of stress.Search is underway for use ofnatural foods for protection of adrenal gland from different types ofinsults.Objective: To determine the protective effects of L-arginine on streptozotocin (STZ)-induced adrenal gland injury in albino rats,andto compare its efficacy to insulin.Material and Methods: This prospective experimental study was done at BMSI, JPMC, Karachi. Forty male, healthy albino rats,90-120 days old were segregated into 4 groups. Group A was marked as control, group B was administered STZ, group C and Dwere treated with STZ along with insulin and L-arginine respectively. At the end of study period, i.e., 6 weeks, animals weresacrificed under ether anaesthesia. Tissue from the left adrenal gland was processed for frozen sectioning to observe fat content ofthe adrenal cortex by applying OilRed O stain.Results: Oil Red-0 stained frozen sections revealed closely aggregated fat globules in adrenal cortex of STZ treated group B ascompared to control. Moderate betterment was seen in group C and in group D Oil Red O stained frozen sections as compared toSTZ treated group B.Conclusion: The results ofthe study demonstrated adrenal cortex injury by STZ which ameliorated with concomitant use of insulinandL-arginine. The protection was more pronounced with L-arginine as comparedto insulin.Keywords:STZ, adrenal gland,insulin,L-arginine

scholarly journals Antioxidant Effects of Oleuropein on Hydrogen Peroxide-Induced Neuronal Stress- An In Vitro Study

2020 ◽  
Vol 19 (1) ◽  
pp. 74-84 ◽  
Author(s):  
Altug Kucukgul ◽  
Mehmet M. Isgor ◽  
Vesile Duzguner ◽  
Meryem N. Atabay ◽  
Azime Kucukgul
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Protective Effects ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Antioxidative Properties ◽  
Total Antioxidant ◽  
Human Glioblastoma Cells

Background: Persistent oxidative stress can lead to chronic inflammation and mediate most chronic diseases including neurological disorders. Oleuropein has been shown to be a potent antioxidant molecule in olive oil leaf having antioxidative properties. Objective: The aim of this study was to investigate the protective effects of oleuropein against oxidative stress in human glioblastoma cells. Methods: Human glioblastoma cells (U87) were pretreated with oleuropein (OP) essential oil 10 µM. After 30 minutes, 100 µM H2O2 was added to the cells for three hours. Cell survival was quantified by colorimetric MTT assay. Glutathione level, total oxidant capacity, total antioxidant capacity and nitric oxide levels were determined by using specific spectrophotometric methods. The relative gene expression level of iNOS was performed by qRT-PCR method. Results: According to viability results, the effective concentration of H2O2 (100µM) significantly decreased cell viability and oleuropein pretreatment significantly prevented the cell losses. Oleuropein regenerated total antioxidant capacity and glutathione levels decreased by H2O2 exposure. In addition, nitric oxide and total oxidant capacity levels were also decreased after administration of oleuropein in treated cells. Conclusion: Oleuropein was found to have potent antioxidative properties in human glioblastoma cells. However, further studies and validations are needed in order to understand the exact neuroprotective mechanism of oleuropein.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected withToxoplasma gondii

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Somayeh Bahrami ◽  
Ali Shahriari ◽  
Mehdi Tavalla ◽  
Somayeh Azadmanesh ◽  
Hossein Hamidinejat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Parasitic Infection ◽  
Chronic Phase ◽  
Antioxidant Defense System ◽  
Female Rats ◽  
Control Group ◽  
Blood Levels ◽  
Total Antioxidant

Toxoplasmosis is a common parasitic infection in the world. Since increased free radicals and oxidative stress are reported in many parasitic diseases the purpose of the present study was to evaluate the oxidative stress in acute and chronic toxoplasmosis. RH strains ofToxoplasmatachyzoites were used in the present study. Twenty-five female rats were infected with the parasite while 25 other rats were as the control group that received normal saline. Zero-, 5-, 7-, 10-, and 45-day postinfection (DPI) blood samples were taken. Some parameters related to oxidant and antioxidants such as antioxidant enzymes, malondialdehyde, and total antioxidant capacity were measured. On day 7 after infection, GPX activity and GSH level were significantly increased and in the mentioned day the amount of total antioxidant capacity was significantly reduced. In other cases, there were no significant differences between the groups in different days. Overall, based on the results it seems that, on day 7 after infection, in infected rats responses to oxidative stress were triggered and led to decrease of total antioxidant capacity. Furthermore, glutathione was increased to cope with stress. It seems that probably antioxidant defense system entered the infection to the chronic phase and changed the parasites stage.

Protective role of silymarin and D-penicillamine against lead-induced liver toxicity and oxidative stress

2017 ◽  
Vol 33 (6) ◽  
pp. 512-518 ◽  
Author(s):  
Seyedeh Missagh Jalali ◽  
Hossein Najafzadeh ◽  
Sadegh Bahmei
Keyword(s):  
Liver Toxicity ◽  
Serum Alkaline Phosphatase ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Antioxidant Defence ◽  
Antioxidant Defence System ◽  
Pb Exposure ◽  
Group 2

This study was performed to assess hepatotoxicity and alterations in liver antioxidant defence in acute lead (Pb) exposure and the protective effects of silymarin in comparison to D-penicillamine in rats. Forty eight Albino rats were divided in eight groups and received the following treatments in a 10-day experiment – group 1: normal saline as control; group 2: 25-mg/kg Pb acetate, intraperitoneally (IP) for the last 5 days; group 3: 100-mg/kg D-penicillamine, IP for the last 5 days; group 4: 200-mg/kg silymarin, orally for 10 days; and groups 5, 6, 7 and 8: in addition to Pb, they received D-penicillamine, for the last 5 days, silymarin for 10 days, a combination of silymarin for 10 days and D-penicillamine for the last 5 days and silymarin for the last 5 days, respectively. Pb acetate exposure induced significant elevation in serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities in group 2 compared to control group. Significant reductions in serum total protein and albumin in all Pb-exposed groups and in serum glucose in groups 2, 6 and 8 were also observed. Liver tissue superoxide dismutase and glutathione peroxidase were significantly lower in groups 2 and 8 compared to control group. Silymarin pretreatment and D-penicillamine administration in groups 5, 7 and 8 could significantly lower ALP, ALT and AST and improve liver antioxidant enzymes. Thus, acute Pb exposure induced hepatotoxicity with suppression of liver antioxidant defence system and silymarin, as an antioxidant could alleviate this effect.

scholarly journals Pengaruh pemberian buah naga merah, jambu biji merah, dan kombinasinya terhadap kapasitas antioksidan total dan kadar malondealdehid pada remaja perokok

2020 ◽  
Vol 9 (1) ◽  
pp. 53-60
Author(s):  
Mohammad Zainul Ma'arif ◽  
Suradi Suradi ◽  
Sugiarto Sugiarto
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Smoking Habits ◽  
Control Group ◽  
Male Adolescent ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Total Antioxidant ◽  
The Mean

Background: Smoking habits enhance free radical in the body, which oxidize lipids to form malondialdehyde. One group that is vulnerable to smoking is adolescents. Red dragon fruit and red guava have the possibility as functional foods due to their vitamin and phytochemical content, which have antioxidant effects. This is an opportunity to prevent oxidative stress among adolescent smokers.Objectives: This study aimed to analyze the effect of red dragon fruit, red guava, and its combination to total antioxidant capacity (TAC) and malondialdehyde (MDA) levels among adolescent mild smokers.Methods: This research was an experimental study with a randomized pre-test and post-test control group design. Forty-eight subjects of male adolescent aged 16-17 years with mild smoking habits were divided into four groups: the control group (K), the intervention group with 200 grams of red dragon fruit (P1), 200 grams of red guava (P2), and the combination of both (100 grams red dragon fruit + 100 grams red guava) (P3), for 14 days. TAC examination used the DPPH method and MDA levels using the TBARS method were measured before and after the intervention.Results: After 14 days of intervention, the mean TAC was changed by -0,10±0,76 in the K group, 0,97±1,62 in the P1 group, 0,74±1,39 in the P2 group, and 0,74±1,29 in the P3 group. But the changes of TAC in all intervention groups were not significantly different from the control group (p>0,05). The mean MDA level changed by 0,229±0,371 in the K group, decreased by -0,654±0,922 in the P1 group, -0,592±0,818 in the P2 group, and -1,166±0,670 in the P3 group. The mean difference of the MDA level in all intervention groups was significantly different from the control group (p<0,05).Conclusion: Consumption of red dragon fruit, red guava, or a combination of red dragon fruit and red guava can reduce malondialdehyde levels significantly among male adolescent mild smokers. Whereas the total antioxidant capacity was changed but not significant.

scholarly journals PO-198 Effect of aerobic exercise on antioxidation ability of mice kidneys

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Sisi He ◽  
Haitao Wang
Keyword(s):  
Aerobic Exercise ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Kidney Tissue ◽  
Exercise Group ◽  
Control Group ◽  
Adult Group ◽  
Total Antioxidant ◽  
The Difference ◽  
Control Group Group

Objective To study the total antioxidant capacity of the kidney in the Aged mice Total Antioxidant Capacity(T-AOC), Malondialdehyde (MDA) and superoxide dismutase (SOD) expression level by aerobic exercise. Objective to explore the effect of aerobic exercise on antioxidation ability of mice. Methods 30 Male C57BL/6 strain mice were randomly divided into adult group ( group CC) in the 13 months old, in the control group (Group C), 5 months of quiet feeding, the aerobic exercise group (Group E) ran for 5 months, 10 per group, and the adult group (group CC) compared with the control group (Group C), The Analysis control Group (Group C) was compared with the aerobic exercise group (Group E). After 5 months of Treadmill running in Group E, Determination of antioxidant index of each group. The statistical method is processed by SPSS20.0 statistic software, the experimental data is expressed by the mean ± standard deviation, the two mean numbers are analyzed by independent sample t test, and the difference of (p<0.05) is statistically significant. Results T-AOC of adult Group was (0.99±0.13) U/mg Prot, control group was (0.27±0.07) u/mg prot, aerobic exercise group (0.39±0.10) U/mg prot, adult group SOD as (46.32± 1.84) U/mg, control Group (29.71±7.64) U/mg, aerobic exercise group (39.85±5.83) U/mg, adult group MDA (8.15±0.16) nmol/mg, control Group (8.83±0.26) nmol/mg, The aerobic exercise group was (6.31±1.23) nmol/mg, the adult group was SOD/MDA (5.69±0.32), the control group was (3.38±0.9) and the aerobic exercise group was (6.55±1.08). Compared with the adult group, the control group T-AOC and SOD decreased significantly, MDA significantly increased, the difference was very significant (p<0.01), sod/mda significantly decreased, SOD/MDA difference was significant (p<0.01). Compared with the control group, the aerobic exercise group T-AOC and SOD obviously increased, MDA significantly decreased, the difference was significant (p<0.01), SOD/MDA increased significantly, the difference was very significant (p<0.01). Conclusions To sum up, with the increase of the age of mice MDA expression is increased, the 18 months old SOD expression is lower than 13 months old, indicating that the antioxidant capacity with the increase in age,  The results of this study are consistent with the results of previous scholars. The results show that aerobic exercise has some antioxidant effect, and aerobic exercise can decrease expression of kidney tissue SOD, raise the expression of kidney tissue T-AOC, increase the expressionof kidney tissue, increase the expression  of SOD/MDA, and reduce the oxidative stress of kidney to some extent,  So as to protect the role of the kidney, aerobic exercise and kidney-related mechanisms also need our further study.

scholarly journals Pengaruh Pemberian Jus Bit (Beta Vulgaris L) Selama Latihan Terhadap Kadar Malondialdehide Dan Status Antioksidan Atlet

2019 ◽  
Vol 3 (2) ◽  
pp. 119
Author(s):  
Fajar Apollo Sinaga ◽  
Rosmaini Hasibuan ◽  
Marsal Risfandi ◽  
Deni Rahman Marpaung ◽  
Jumadin IP ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Beta Vulgaris ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Antioxidant ◽  
Randomized Control

Aktifitas fisik berat dapat menyebabkan peroksidasi lipid ditandai peningkatan kadar malondialdehyde (MDA) dan penurunan kadar antioksidan yang dapat menurunkan performance dan kesehatan atlet. Peroksidasi lipid dapat dicegah atau dikurangi dengan pemberian antioksidan. Bit (Beta Vulgaris L) mengandung berbagai jenis antioksidan alami tetapi belum pernah diteliti khasiatnya untuk mengurangi peroksidasi lipid yang dipicu oleh aktifitas fisik. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian jus bit selama latihan terhadap peroksidasi lipid dan status antioksidan pada saat melakukan aktifitas fisik maksimal. Jenis penelitian yang dilakukan adalah penelitian eksperimental dengan rancangan penelitian Randomized Control Group Pretest-Postest Design. Penelitian dilaksanakan di Stadion  Unimed dan Laboratorium Terpadu Fakultas Kedokteran USU. Sampel penelitian adalah mahasiswa Ilmu Keolahragaan sebanyak 30 orang  yang memenuhi kriteria. Pretest dilakukan dengan memeriksa kadar MDA, Total Antioxidant Capacity (TAC) dan VO2max. Selanjutnya sampel dibagi atas 2 kelompok  (P1=15, P2=15). Selama menjalani program latihan  kelompok P1 diberi Jus Bit 300 ml 1x1 hari, sedangkan P2 diberi sebagai kontrol. Kemudian semua sampel melakukan aktifitas fisik maksimal dengan menggunakan bleep test, dan kembali dilakukan pemeriksaan kadar MDA, TAC dan VO2max. Hasil penelitian menunjukkan terdapat penurunan kadar MDA serta peningkatan kadar TAC dan VO2max pada kelompok pelatihan yang diberi jus bit 300ml dibandingkan dengan kelompok kontrol (p<0,05). Kesimpulan penelitian adalah pemberian jus bit selama latihan dapat menurunkan kadar MDA serta meningkatkan kadar TAC dan VO2max atlet. Kata kunci: Jus Bit, Malondialdehyde, Antioksidan, Aktivitas Fisik Maksimal, Latihan

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