Possible cardioprotective role of NaHS on ECG and oxidative stress markers in unpredictable chronic mild stress model in rats
The protective effect of H2S against various body organ injuries has been described. The aim of this work is to investigate the potential role of sodium hydrosulfide; NaHS as an H2S donor, in chronic mild stress-induced changes in the heart of rat. Forty adult male Sprague Dawley rats were assigned into four groups: control, stressed group, stressed rats treated with Amino-oxyacetic acid (AOAA), and stressed rats treated with NaHS. Arterial blood pressure (ABP) was recorded. Serum adrenaline, MDA and GSH levels were measured. Chronic stress significantly increased HR and ABP. AOAA produced similar changes, while NaHS mitigated the rise in HR and ABP. Both stressed and AOAA groups showed a significant decrease in QRS amplitude, shortening of R-R, Q-T interval and Q-Tc with an elevation of S-T segment. NaHS produced a significant improvement in ECG recordings. Chronic stress produced a significant rise of adrenaline and MDA levels with a significant decline in GSH level. AOAA group showed similar elevations. NaHS caused significant reduction in adrenaline and MDA levels but significantly improved GSH level. In conclusion, H2S donor has a cardio-protective effect against stress-induced cardiovascular diseases through amelioration of the oxidative stress and raised adrenaline level induced by chronic stress exposure.