STUDIES ON ANTIOXIDANT ACTIVITY: I. ESTIMATION OF ANTIOXIDANT ACTIVITY IN STABILIZING VITAMIN A IN OILS

1941 ◽  
Vol 19b (1) ◽  
pp. 17-23 ◽  
Author(s):  
W. E. Parker ◽  
A. C. Neish ◽  
W. D. McFarlane

The efficiency of wheat-germ oil in stabilizing vitamin A against the destructive action of ultra-violet radiations was investigated. A rapid and convenient method of estimating antioxidant potency is described which is based on ultraviolet irradiation of mixtures of halibut liver oil and antioxidant in chloroform solution and estimation of vitamin A by the direct application of the antimony trichloride reaction employing photoelectric colorimetry.

1950 ◽  
Vol 28f (6) ◽  
pp. 157-165 ◽  
Author(s):  
H. J. Lips ◽  
W. D. McFarlane

Accelerated tests for antioxidant activity are described which depend on the protection of vitamin A concentrates (in ethylene dichloride solution) against ultraviolet irradiation, and on the protection of carotene (in mineral oil solution) against heat and aeration. The protection of vitamin A was influenced by light-filtering effects; the carotene method appeared to be more generally useful. The stabilizing power of wheat-germ oil for both solutions of Vitamin A and carotene against light is partly due to a screening effect by pigments. Some of the protection is also due to other components of the wheat-germ oil, but it is not known whether these function as stabilizers against light according to the usual theories of antioxidant action. Light-accelerated decomposition of vitamin A and carotene appeared more suitable for special tests than for general evaluation of antioxidants; stability experiments in the absence of light gave a more generally applicable picture of antioxidant activity.


Author(s):  
Hadeer Zakaria ◽  
Tarek M. Mostafa ◽  
Gamal A. El-Azab ◽  
Nagy AH Sayed-Ahmed

Abstract. Background: Elevated homocysteine levels and malnutrition are frequently detected in hemodialysis patients and are believed to exacerbate cardiovascular comorbidities. Omega-3 fatty acids have been postulated to lower homocysteine levels by up-regulating metabolic enzymes and improving substrate availability for homocysteine degradation. Additionally, it has been suggested that prevention of folate depletion by vitamin E consumption decreases homocysteine levels. However, data on the effect of omega-3 fatty acids and/or vitamin E on homocysteine levels and nutritional status have been inconclusive. Therefore, this study was planned to examine the effect of combined supplementation of fish oil, as a source of omega-3 fatty acids, with wheat germ oil, as a source of vitamin E, on homocysteine and nutritional indices in hemodialysis patients. Methods: This study was a randomized, double-blind, placebo-controlled trial. Forty-six hemodialysis patients were randomly assigned to two equally-sized groups; a supplemented group who received 3000 mg/day of fish oil [1053 mg omega-3 fatty acids] plus 300 mg/day of wheat germ oil [0.765 mg vitamin E], and a matched placebo group who received placebo capsules for 4 months. Serum homocysteine and different nutritional indices were measured before and after the intervention. Results: Twenty patients in each group completed the study. At the end of the study, there were no significant changes in homocysteine levels and in the nutritional indices neither in the supplemented nor in the placebo-control groups (p > 0.05). Conclusions: Fish oil and wheat germ oil combination did not produce significant effects on serum homocysteine levels and nutritional indices of hemodialysis patients.


2021 ◽  
Author(s):  
Anam Khalid ◽  
Muhammad Sohaib ◽  
Muhammad Tahir Nadeem ◽  
Farhan Saeed ◽  
Ali Imran ◽  
...  

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 662
Author(s):  
Sabrin Abdelrahman Morshedy ◽  
Ahmed M. Abdelmodather ◽  
Mohamed M. Basyony ◽  
Soliman A. Zahran ◽  
Mohamed A. Hassan

Vegetable oils are a source of natural antioxidants, including tocopherols, sterols, phenolic compounds, coenzymes, and polyunsaturated fatty acids that provide nutritional value, organoleptic properties, and significantly delay or prevent lipid oxidation. Eighty-four V-line rabbits at 5 weeks of age with an initial body weight (BW) of 535.60 ± 13.48 g were assigned randomly to four experimental groups (seven replicates in each group with three rabbits each). The first group served as a control and received 0.3 mL/kg BW of distilled water (CON), while the second and third groups received 0.3 mL/kg BW of rocket seed oil (RSO) and wheat germ oil (WGO), respectively. The fourth group received a mixture of oils consisting of 0.15 mL of RSO and 0.15 mL of WGO/kg BW (MOs). The experiment lasted 7 weeks. The study investigated the effects of RSO, WGO, and their mixture on growth performance, feed utilization, antioxidant status, and immune response of growing rabbits. The results indicated that the rabbits that were administered orally with RSO and WGO or their mixture had higher (p ≤ 0.05) final BW, weight gain, and average daily gain when compared to the control group. In addition, the feed conversion ratio improved significantly with RSO, WGO, and MOs treatments. Different oil treatments improved nutrient digestibility, nutritive value, and nitrogen balance. Moreover, the rabbits that received RSO, WGO, and their mixture had an improvement the meat fatty acid composition compared to the control rabbits. Oral administration of RSO, WGO, and their mixture significantly improved serum protein fractions, decreased blood urea nitrogen, and had a positive effect on serum total lipids, HDL-c, and LDL-c. Furthermore, the treatments of RSO, WGO, and MOs had a significant improvement in the antioxidative status and immune response.


Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1106
Author(s):  
Marko R. Antonijević ◽  
Dušica M. Simijonović ◽  
Edina H. Avdović ◽  
Andrija Ćirić ◽  
Zorica D. Petrović ◽  
...  

Compounds from the plant world that possess antioxidant abilities are of special importance for the food and pharmaceutical industry. Coumarins are a large, widely distributed group of natural compounds, usually found in plants, often with good antioxidant capacity. The coumarin-hydroxybenzohydrazide derivatives were synthesized using a green, one-pot protocol. This procedure includes the use of an environmentally benign mixture (vinegar and ethanol) as a catalyst and solvent, as well as very easy isolation of the desired products. The obtained compounds were structurally characterized by IR and NMR spectroscopy. The purity of all compounds was determined by HPLC and by elemental microanalysis. In addition, these compounds were evaluated for their in vitro antioxidant activity. Mechanisms of antioxidative activity were theoretically investigated by the density functional theory approach and the calculated values of various thermodynamic parameters, such as bond dissociation enthalpy, proton affinity, frontier molecular orbitals, and ionization potential. In silico calculations indicated that hydrogen atom transfer and sequential proton loss–electron transfer reaction mechanisms are probable, in non-polar and polar solvents respectively. Additionally, it was found that the single-electron transfer followed by proton transfer was not an operative mechanism in either solvent. The conducted tests indicate the excellent antioxidant activity, as well as the low potential toxicity, of the investigated compounds, which makes them good candidates for potential use in food chemistry.


1940 ◽  
Vol 18b (12) ◽  
pp. 405-409 ◽  
Author(s):  
W. E. Parker ◽  
W. D. McFarlane

By treating a petrol ether solution of wheat-germ oil with 85% sulphuric acid, centrifuging, and washing the supernatant petrol ether with dilute alkali, carotenoids and other substances that interfere with the iron-dipyridyl method for determining tocopherol are removed. Tocopherol is not affected by the treatment. The tocopherol content of the solution is finally determined by a modification of Emmerie's method.


1962 ◽  
Vol 40 (3) ◽  
pp. 371-374 ◽  
Author(s):  
Claude Ritchot ◽  
J. E. McFarlane

Lipid other than sterol improves the growth and development of nymphs of Acheta domesticus (L.), as shown by the effect of adding wheat germ oil and linoleic acid to the diet. Such lipid is also necessary for reproduction: the effect appears to be on the male, but may also be on the female.


Science ◽  
1936 ◽  
Vol 83 (2157) ◽  
pp. 421-421
Author(s):  
Oliver H. Emerson ◽  
Gladys A. Emerson ◽  
Herbert M. Evans

2021 ◽  
Vol 24 (6) ◽  
pp. 706-715
Author(s):  
Raghda Zakaria ◽  
Raad J. Musa ◽  
Jabar Faraj ◽  
Zead H. Mahmoud ◽  
Hamdoon A. Mohamme

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